Rorug05G0434000

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004995
Physical Location & Seq
Forward (+)
60294497 .. 60297165
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0434000.1

Sequence Viewer

Length: 1797 bp
ATGCTAAGGAAGTCGCTGCGCACAGCTGCTTCTTCTTCTTATTGCAGCAGAGGAGGCATGTCTTCTCTTCACTCTCCTCTTGTTGTTTTCCTCTGCAATCACTTGGCCTCGTTTCATTCTCAACCTTCAAATCCAATCGAACCCAGAAACACCCAGATTGTGAGAGACCCACCCGTACCCAAACTAACTAACATTGAGGATGCCTTGAAGGTGTTCGATGAAATGCTTCAAAGGCGTCCTCTTCCTTCCATTATCCGTTTCAATCAAATCTTGTGTCAACTCATGAAATTGAAGTACTATTCCGAAGTCATTTCTTTGTATCATCAATTGGGCCTCCTGGGAATTGTTCCTAACGATTTTAGTCTCAACATTATCATTAACTGCCATTGTCGTTTTAACCAAATGGGGTTTGCTTTATCTGTCTTCGGAAAATTCTTCAAATTTGGTTTTGAACCGGATGTCACCACCCTCAACACTCTAATCAATGGCTTTCTTCTTGAAGATAGGGTGGCTGAGGCAGCAGGATTTTTTAGCAGAATGGTTGAGGGAAGTAACTGTAAGCCTGATGTGTTTACTTTCGGCGCACTAGTTAAGGGCTTGTGCATCAAGGGTAACAATACTGCAGCTATTCGGTTGCTTAGGAAGATGGATGAAGGAGCATGTAAGCCTAACCTTGTTGTGTATAACACGATCATCGACAGTCTGTGTAAGGATACACTCATTGTTGATGCACAGGAGCTCTTCTCGGAAATGATCAGTAGGGGTATTGCCCCGGACATTGTTACGTATAACTCTTTGATTCATGGCGTTTGCAGAGTAGGCAAGTGGAAAGAAGCTATAAGGTTGTTGAATGAAATGGTGAGTAACGATATCTTTCCAGATATACTCACCTTTAACATCTTGGTAGATACACTTTGTAAGGAGGGGTTGGTATTGCAAGCAGAAAGTGTGGTTCAAATGATGATTCAAAGAGGTATTGAACCTGATACGGTTACCTACAGTTCGCTTATGGATGGCTACTGTCTGCGAGGAGAAATGGATGAGGCAAAAAAAGTATTTGATAAAATGATTAGCAAGGGTTCCAGGGTTGATGTTTTTTGTTATTCCATAATGATAAATGGATATTGTAAGCGTAAAATGGTTGATGAGGCAGTGATGCTTTTTCAGGAAATATCTCAAATGGGGCTGGTTCCAAATACCATTACTTATAACGCTCTCATTGACGGTTTTTGCAAAATGGGCAGGATACAAGGCGCACGAAAGTTATTCTCGAAGATGCAAGCTTGCGGCCAGCTTCCAGATGTTCAAACTTATGCTATTCTACTGGATGGGTTATGTAAAAACCAACAACTTTCTTTGGCAATAGAATTGTTGAGAGAGATGGAAGACAGAAAGATGGATCTAAATATTGTAATTTACAGTATTCTTATTGAAGGTTTGTGCATAGTTGGAAATATTGCAGCTGCAATAGGTCTGTTCCGTTGTTTACCATCAAAACGACTACAACCTGATGTGAAGATGTACACCATAATGATCAGTGGATTTTGTAACGGGGGGTTAACAAGTGAAGCCGAAAAGTTGTTTAAGGAGATGGAAGATAAAGACTGTCCTCCTGATGCTTGCACCTATAACATAATTATCCGCGGGTTTATCAATAACAATGAGACATTAAGGGCAATGGGACTTATTCAACAGATGGTGGAGAGGGGCTTCGCGGCAGATGCATCCACTGTAGAACTGATTGTTGGTCTACTATGTAAAGATAACGTTGATCCCGCTTTATTGACTCTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

598

Amino Acids

66.84

Weight (kDa)

6.37

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 117 - 164 3.5e-08 PPR repeat family
PPR_1 PF12854 149 - 181 8.6e-06 PPR repeat
PPR_2 PF13041 152 - 201 8.9e-11 PPR repeat family
PPR_3 PF13812 182 - 233 3.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 185 - 216 1.6e-08 PPR repeat
PPR_2 PF13041 196 - 237 4.6e-08 PPR repeat family
PPR_long PF17177 206 - 337 4.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 221 - 251 1.5e-10 PPR repeat
PPR_2 PF13041 223 - 272 1e-16 PPR repeat family
PPR PF01535 226 - 256 3.7e-06 PPR repeat
PPR_3 PF13812 247 - 305 7.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 254 - 287 7.4e-14 PPR repeat
PPR_2 PF13041 258 - 307 9.5e-19 PPR repeat family
PPR PF01535 261 - 291 1.5e-08 PPR repeat
PPR_1 PF12854 290 - 321 1.7e-07 PPR repeat
PPR_2 PF13041 293 - 341 6.9e-14 PPR repeat family
PPR_3 PF13812 316 - 377 2.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 324 - 356 1.3e-13 PPR repeat
PPR_2 PF13041 328 - 377 1.4e-16 PPR repeat family
PPR PF01535 331 - 360 2.1e-09 PPR repeat
PPR_3 PF13812 351 - 410 1.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 359 - 390 2.9e-09 PPR repeat
PPR_2 PF13041 364 - 412 6.4e-17 PPR repeat family
PPR PF01535 367 - 396 1.9e-07 PPR repeat
TPR_24 PF23276 388 - 494 1.1e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 394 - 427 3.8e-13 PPR repeat
PPR_2 PF13041 398 - 447 2.5e-18 PPR repeat family
PPR PF01535 401 - 430 2.2e-08 PPR repeat
PPR_1 PF12854 430 - 462 1.6e-08 PPR repeat
PPR_2 PF13041 440 - 481 7.4e-09 PPR repeat family
PPR_2 PF13041 469 - 517 5.7e-10 PPR repeat family
PPR_3 PF13812 498 - 550 7.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 500 - 532 3.6e-10 PPR repeat
PPR_2 PF13041 503 - 551 7.9e-16 PPR repeat family
PPR PF01535 507 - 536 2.6e-08 PPR repeat
PPR_2 PF13041 538 - 587 7.3e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1209
Acc16I TGCGCA 1 cut(s) 20
AccI GTMKAC 1 cut(s) 1750
AccII CGCG 2 cut(s) 1644, 1715
AciI CCGC 5 cut(s) 1287, 1642, 1644, 1715, 1776
AclI AACGTT 1 cut(s) 1767
AclWI GGATC 2 cut(s) 1407, 1766
AcoI YGGCCR 1 cut(s) 1288
AcsI RAATTY 2 cut(s) 431, 440
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 3 cut(s) 177, 296, 1523
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 2 cut(s) 336, 1082
AjuI GAANNNNNNNTTGG 2 cut(s) 1728, 1760
AluBI AGCT 7 cut(s) 26, 626, 739, 836, 1283, 1294, 1463
AluI AGCT 7 cut(s) 26, 626, 739, 836, 1283, 1294, 1463
Alw21I GWGCWC 1 cut(s) 741
Alw26I GTCTC 3 cut(s) 159, 368, 1658
AlwI GGATC 2 cut(s) 1407, 1766
AoxI GGCC 3 cut(s) 105, 331, 1288
ApeKI GCWGC 7 cut(s) 16, 26, 45, 518, 623, 1460, 1463
ApoI RAATTY 2 cut(s) 431, 440
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
Asp700I GAANNNNTTC 2 cut(s) 212, 225
AspLEI GCGC 3 cut(s) 21, 584, 1256
AspS9I GGNCC 1 cut(s) 331
AsuC2I CCSGG 1 cut(s) 773
AsuHPI GGTGA 3 cut(s) 454, 871, 880
BanII GRGCYC 1 cut(s) 741
BbsI GAAGAC 3 cut(s) 54, 415, 1392
Bbv12I GWGCWC 1 cut(s) 741
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 7 cut(s) 3, 13, 57, 530, 635, 1450, 1472
BccI CCATC 8 cut(s) 640, 1007, 1322, 1375, 1390, 1498, 1585, 1690
BciT130I CCWGG 2 cut(s) 338, 1084
BciVI GTATCC 2 cut(s) 706, 1239
BclI TGATCA 2 cut(s) 753, 1533
BcnI CCSGG 1 cut(s) 773
BcoDI GTCTC 3 cut(s) 159, 368, 1658
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 3 cut(s) 621, 997, 1731
BfuI GTATCC 2 cut(s) 706, 1239
BisI GCNGC 9 cut(s) 17, 27, 46, 519, 624, 1288, 1461, 1464, 1716
BlsI GCNGC 9 cut(s) 18, 28, 47, 520, 625, 1289, 1462, 1465, 1717
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 3 cut(s) 338, 773, 1084
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 2 cut(s) 1081, 1191
BmrFI CCNGG 3 cut(s) 338, 773, 1084
BmsI GCATC 8 cut(s) 190, 612, 718, 1146, 1266, 1606, 1711, 1733
BpiI GAAGAC 3 cut(s) 54, 415, 1392
BplI GAGNNNNNCTC 2 cut(s) 728, 760
Bpu10I CCTNAGC 3 cut(s) 5, 513, 638
BpuMI CCSGG 1 cut(s) 773
BsaAI YACGTR 1 cut(s) 786
BsaHI GRCGYC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 4 cut(s) 337, 771, 1083, 1642
BsaWI WCCGGW 1 cut(s) 454
Bse1I ACTGG 1 cut(s) 1329
Bse3DI GCAATG 1 cut(s) 1683
BseBI CCWGG 2 cut(s) 338, 1084
BseDI CCNNGG 4 cut(s) 337, 771, 1083, 1642
BseGI GGATG 7 cut(s) 205, 463, 655, 1018, 1045, 1333, 1724
BseMI GCAATG 1 cut(s) 1683
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 1 cut(s) 1329
BseRI GAGGAG 3 cut(s) 66, 66, 1044
BseXI GCAGC 7 cut(s) 3, 13, 57, 530, 635, 1450, 1472
Bsh1236I CGCG 2 cut(s) 1644, 1715
BshFI GGCC 3 cut(s) 107, 333, 1290
BsiHKAI GWGCWC 1 cut(s) 741
BsiSI CCGG 2 cut(s) 455, 773
BslFI GGGAC 1 cut(s) 1695
BsmAI GTCTC 3 cut(s) 159, 368, 1658
BsmFI GGGAC 1 cut(s) 1695
BsnI GGCC 3 cut(s) 107, 333, 1290
Bso31I GGTCTC 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 741
Bsp1407I TGTACA 1 cut(s) 1521
Bsp143I GATC 5 cut(s) 690, 753, 1399, 1533, 1771
BspACI CCGC 5 cut(s) 1287, 1642, 1644, 1715, 1776
BspANI GGCC 3 cut(s) 107, 333, 1290
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 2 cut(s) 1644, 1715
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 1081, 1191
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 2 cut(s) 1407, 1766
BspQI GCTCTTC 1 cut(s) 746
BspTNI GGTCTC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 1683
BsrGI TGTACA 1 cut(s) 1521
BsrI ACTGG 1 cut(s) 1329
BssECI CCNNGG 4 cut(s) 337, 771, 1083, 1642
BssMI GATC 5 cut(s) 690, 753, 1399, 1533, 1771
BssNI GRCGYC 1 cut(s) 235
Bst2UI CCWGG 2 cut(s) 338, 1084
Bst4CI ACNGT 9 cut(s) 557, 701, 991, 1001, 1022, 1226, 1421, 1607, 1732
Bst6I CTCTTC 3 cut(s) 72, 246, 746
BstACI GRCGYC 1 cut(s) 235
BstAUI TGTACA 1 cut(s) 1521
BstBAI YACGTR 1 cut(s) 786
BstC8I GCNNGC 5 cut(s) 939, 1281, 1285, 1292, 1621
BstDEI CTNAG 3 cut(s) 5, 513, 638
BstDSI CCRYGG 1 cut(s) 1642
BstEII GGTNACC 1 cut(s) 991
BstF5I GGATG 7 cut(s) 205, 463, 655, 1018, 1045, 1333, 1724
BstFNI CGCG 2 cut(s) 1644, 1715
BstHHI GCGC 3 cut(s) 21, 584, 1256
BstKTI GATC 5 cut(s) 693, 756, 1402, 1536, 1774
BstMAI GTCTC 3 cut(s) 159, 368, 1658
BstMBI GATC 5 cut(s) 690, 753, 1399, 1533, 1771
BstMWI GCNNNNNNNGC 6 cut(s) 54, 232, 518, 819, 1239, 1721
BstNI CCWGG 2 cut(s) 338, 1084
BstNSI RCATGY 2 cut(s) 61, 663
BstPI GGTNACC 1 cut(s) 991
BstSCI CCNGG 3 cut(s) 336, 771, 1082
BstSFI CTRYAG 3 cut(s) 621, 997, 1731
BstSNI TACGTA 1 cut(s) 786
BstUI CGCG 2 cut(s) 1644, 1715
BstV1I GCAGC 7 cut(s) 3, 13, 57, 530, 635, 1450, 1472
BstV2I GAAGAC 3 cut(s) 54, 415, 1392
BstX2I RGATCY 1 cut(s) 1399
BstYI RGATCY 1 cut(s) 1399
BsuI GTATCC 2 cut(s) 706, 1239
BsuRI GGCC 3 cut(s) 107, 333, 1290
BtgI CCRYGG 1 cut(s) 1642
BtsCI GGATG 7 cut(s) 205, 463, 655, 1018, 1045, 1333, 1724
BtsI GCAGTG 1 cut(s) 1158
BtsIMutI CAGTG 3 cut(s) 1158, 1543, 1728
Cac8I GCNNGC 5 cut(s) 939, 1281, 1285, 1292, 1621
CciI TCATGA 1 cut(s) 282
CfoI GCGC 3 cut(s) 21, 584, 1256
Cfr13I GGNCC 1 cut(s) 331
Cfr42I CCGCGG 1 cut(s) 1645
CseI GACGC 1 cut(s) 224
Csp6I GTAC 3 cut(s) 176, 295, 1522
CviAII CATG 4 cut(s) 58, 283, 660, 803
CviQI GTAC 3 cut(s) 176, 295, 1522
DdeI CTNAG 3 cut(s) 5, 513, 638
DpnI GATC 5 cut(s) 692, 755, 1401, 1535, 1773
DpnII GATC 5 cut(s) 690, 753, 1399, 1533, 1771
EaeI YGGCCR 1 cut(s) 1288
Eam1104I CTCTTC 3 cut(s) 72, 246, 746
EarI CTCTTC 3 cut(s) 72, 246, 746
Ecl136II GAGCTC 1 cut(s) 739
Eco105I TACGTA 1 cut(s) 786
Eco24I GRGCYC 1 cut(s) 741
Eco31I GGTCTC 1 cut(s) 159
Eco32I GATATC 1 cut(s) 871
Eco53kI GAGCTC 1 cut(s) 739
Eco91I GGTNACC 1 cut(s) 991
EcoICRI GAGCTC 1 cut(s) 739
EcoO65I GGTNACC 1 cut(s) 991
EcoRII CCWGG 2 cut(s) 336, 1082
EcoRV GATATC 1 cut(s) 871
EcoT22I ATGCAT 1 cut(s) 1726
EcoT38I GRGCYC 1 cut(s) 741
FaeI CATG 4 cut(s) 61, 286, 663, 806
FaqI GGGAC 1 cut(s) 1695
FatI CATG 4 cut(s) 57, 282, 659, 802
FauI CCCGC 2 cut(s) 1637, 1783
FbaI TGATCA 2 cut(s) 753, 1533
FblI GTMKAC 1 cut(s) 1750
Fnu4HI GCNGC 9 cut(s) 17, 27, 46, 519, 624, 1288, 1461, 1464, 1716
FokI GGATG 7 cut(s) 212, 470, 662, 1025, 1052, 1340, 1711
FriOI GRGCYC 1 cut(s) 741
Fsp4HI GCNGC 9 cut(s) 17, 27, 46, 519, 624, 1288, 1461, 1464, 1716
FspBI CTAG 1 cut(s) 587
FspI TGCGCA 1 cut(s) 20
GlaI GCGC 3 cut(s) 20, 583, 1255
GluI GCNGC 9 cut(s) 17, 27, 46, 519, 624, 1288, 1461, 1464, 1716
HaeIII GGCC 3 cut(s) 107, 333, 1290
HapII CCGG 2 cut(s) 455, 773
HgaI GACGC 1 cut(s) 224
HhaI GCGC 3 cut(s) 21, 584, 1256
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 4 cut(s) 61, 286, 663, 806
Hin6I GCGC 3 cut(s) 19, 582, 1254
HinP1I GCGC 3 cut(s) 19, 582, 1254
HincII GTYRAC 2 cut(s) 278, 1560
HindII GTYRAC 2 cut(s) 278, 1560
HindIII AAGCTT 1 cut(s) 1281
HinfI GANTC 3 cut(s) 799, 964, 1786
HpaI GTTAAC 1 cut(s) 1560
HpaII CCGG 2 cut(s) 455, 773
HphI GGTGA 3 cut(s) 454, 871, 880
Hpy166II GTNNAC 6 cut(s) 278, 573, 1487, 1524, 1560, 1751
Hpy188I TCNGA 3 cut(s) 304, 428, 748
Hpy188III TCNNGA 7 cut(s) 283, 497, 878, 1166, 1270, 1298, 1613
Hpy8I GTNNAC 6 cut(s) 278, 573, 1487, 1524, 1560, 1751
HpyAV CCTTC 5 cut(s) 135, 202, 255, 647, 1427
HpyCH4III ACNGT 9 cut(s) 557, 701, 991, 1001, 1022, 1226, 1421, 1607, 1732
HpyCH4IV ACGT 2 cut(s) 785, 1767
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 232, 518, 819, 1239, 1721
HpyF3I CTNAG 3 cut(s) 5, 513, 638
HpySE526I ACGT 2 cut(s) 785, 1767
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 4 cut(s) 61, 286, 663, 806
HspAI GCGC 3 cut(s) 19, 582, 1254
Ksp22I TGATCA 2 cut(s) 753, 1533
KspAI GTTAAC 1 cut(s) 1560
KspI CCGCGG 1 cut(s) 1645
Kzo9I GATC 5 cut(s) 690, 753, 1399, 1533, 1771
LguI GCTCTTC 1 cut(s) 746
LmnI GCTCC 2 cut(s) 656, 736
Lsp1109I GCAGC 7 cut(s) 3, 13, 57, 530, 635, 1450, 1472
LweI GCATC 8 cut(s) 190, 612, 718, 1146, 1266, 1606, 1711, 1733
MaeI CTAG 1 cut(s) 587
MaeII ACGT 2 cut(s) 785, 1767
MaeIII GTNAC 7 cut(s) 460, 551, 611, 781, 863, 991, 1547
MalI GATC 5 cut(s) 692, 755, 1401, 1535, 1773
MboI GATC 5 cut(s) 690, 753, 1399, 1533, 1771
MfeI CAATTG 1 cut(s) 326
MflI RGATCY 1 cut(s) 1399
MhlI GDGCHC 1 cut(s) 741
MluCI AATT 8 cut(s) 287, 326, 342, 431, 440, 1367, 1413, 1635
MlyI GAGTC 1 cut(s) 1780
MmeI TCCRAC 1 cut(s) 1429
Mph1103I ATGCAT 1 cut(s) 1726
MroXI GAANNNNTTC 2 cut(s) 212, 225
MseI TTAA 7 cut(s) 378, 396, 591, 894, 1559, 1584, 1670
MslI CAYNNNNRTG 1 cut(s) 1529
MspA1I CMGCKG 3 cut(s) 26, 1463, 1644
MspI CCGG 2 cut(s) 455, 773
MspR9I CCNGG 3 cut(s) 338, 773, 1084
MunI CAATTG 1 cut(s) 326
MvaI CCWGG 2 cut(s) 338, 1084
MvnI CGCG 2 cut(s) 1644, 1715
MwoI GCNNNNNNNGC 6 cut(s) 54, 232, 518, 819, 1239, 1721
NciI CCSGG 1 cut(s) 773
NdeII GATC 5 cut(s) 690, 753, 1399, 1533, 1771
NlaIII CATG 4 cut(s) 61, 286, 663, 806
NlaIV GGNNCC 2 cut(s) 1081, 1191
NmuCI GTSAC 1 cut(s) 460
NsbI TGCGCA 1 cut(s) 20
NsiI ATGCAT 1 cut(s) 1726
NspI RCATGY 2 cut(s) 61, 663
PagI TCATGA 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 746
PdmI GAANNNNTTC 2 cut(s) 212, 225
PfeI GAWTC 2 cut(s) 799, 964
PkrI GCNGC 9 cut(s) 18, 28, 47, 520, 625, 1289, 1462, 1465, 1717
PleI GAGTC 1 cut(s) 1780
PpsI GAGTC 1 cut(s) 1780
Ppu21I YACGTR 1 cut(s) 786
PsiI TTATAA 1 cut(s) 1209
Psp124BI GAGCTC 1 cut(s) 741
Psp1406I AACGTT 1 cut(s) 1767
Psp6I CCWGG 2 cut(s) 336, 1082
PspEI GGTNACC 1 cut(s) 991
PspGI CCWGG 2 cut(s) 336, 1082
PspN4I GGNNCC 2 cut(s) 1081, 1191
PspPI GGNCC 1 cut(s) 331
PstI CTGCAG 1 cut(s) 625
PsuI RGATCY 1 cut(s) 1399
PvuII CAGCTG 2 cut(s) 26, 1463
RsaI GTAC 3 cut(s) 177, 296, 1523
RsaNI GTAC 3 cut(s) 176, 295, 1522
RseI CAYNNNNRTG 1 cut(s) 1529
SacI GAGCTC 1 cut(s) 741
SacII CCGCGG 1 cut(s) 1645
SapI GCTCTTC 1 cut(s) 746
SaqAI TTAA 7 cut(s) 378, 396, 591, 894, 1559, 1584, 1670
SatI GCNGC 9 cut(s) 17, 27, 46, 519, 624, 1288, 1461, 1464, 1716
Sau3AI GATC 5 cut(s) 690, 753, 1399, 1533, 1771
Sau96I GGNCC 1 cut(s) 331
ScaI AGTACT 1 cut(s) 296
SchI GAGTC 1 cut(s) 1780
ScrFI CCNGG 3 cut(s) 338, 773, 1084
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 8 cut(s) 190, 612, 718, 1146, 1266, 1606, 1711, 1733
SfcI CTRYAG 3 cut(s) 621, 997, 1731
Sfr303I CCGCGG 1 cut(s) 1645
SgrBI CCGCGG 1 cut(s) 1645
SmiMI CAYNNNNRTG 1 cut(s) 1529
SnaBI TACGTA 1 cut(s) 786
SpeI ACTAGT 1 cut(s) 586
Sse9I AATT 8 cut(s) 287, 326, 342, 431, 440, 1367, 1413, 1635
SsiI CCGC 5 cut(s) 1287, 1642, 1644, 1715, 1776
SspI AATATT 2 cut(s) 1408, 1456
SspMI CTAG 1 cut(s) 587
SstI GAGCTC 1 cut(s) 741
StyD4I CCNGG 3 cut(s) 336, 771, 1082
TaaI ACNGT 9 cut(s) 557, 701, 991, 1001, 1022, 1226, 1421, 1607, 1732
TaiI ACGT 2 cut(s) 788, 1770
TaqI TCGA 4 cut(s) 138, 216, 696, 1271
TasI AATT 8 cut(s) 287, 326, 342, 431, 440, 1367, 1413, 1635
TatI WGTACW 2 cut(s) 294, 1521
TauI GCSGC 2 cut(s) 1290, 1718
TfiI GAWTC 2 cut(s) 799, 964
Tru1I TTAA 7 cut(s) 378, 396, 591, 894, 1559, 1584, 1670
Tru9I TTAA 7 cut(s) 378, 396, 591, 894, 1559, 1584, 1670
TscAI CASTG 3 cut(s) 1158, 1543, 1735
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 7 cut(s) 16, 26, 45, 518, 623, 1460, 1463
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 6 cut(s) 104, 234, 299, 666, 791, 867
TspGWI ACGGA 2 cut(s) 245, 1469
TspRI CASTG 3 cut(s) 1158, 1543, 1735
XapI RAATTY 2 cut(s) 431, 440
XceI RCATGY 2 cut(s) 61, 663
XmiI GTMKAC 1 cut(s) 1750
XmnI GAANNNNTTC 2 cut(s) 212, 225
XspI CTAG 1 cut(s) 587
ZrmI AGTACT 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 1726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.