Rh5BG513300

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004995
Physical Location & Seq
Forward (+)
81428669 .. 81434944
6276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG513300.1

Sequence Viewer

Length: 681 bp
ATGGGGGCTGTAGTGGGAAAGGCCAATAGTGGGAGTCTTTCGATATCTGAAACAAAGCTGGAAGCCAAAATGGTTGAAGCTATGCAGCGAAGAGCAGCTAAAGGAAGTGTCATGAAATCATTCAACAGCATAATCTTGAAGTTCCCAAAGATTGATGAGAGCTTAAGAAATTGCAAAGATATTTTTCAGCAGTTTGATGAGGATTCAAATGGCATCATTGATCATGATGAATTGAAAAGAGCTTTCTCAAAACTGGAAGTCTCGTTTACAGATGAGGAAATCGATGATCTCTTTGCAGCATGCGATATTAATGAGGATATGGGAATCAATTTTAATGAGTTCATCGTTCTTATTTGCGTCGTCTTTCTTCTGAAGGATGATCAGAATGCTGGGCAAAAAAAAGTGGATATGGGAATGCCAAGGCTGGAAGCCACCTTCGAAACATTGGTGGATGCATTTGTGTTCTTGGATAAGAACAAGGATGGTTATGTCAGCAAGAGTGAAATGGTTCAGTCCATTAATGAAACTACGACAGGAGAACGCTCCTCTGGGCGAATAGCCATGAAAAGATTTGAAGAGATGGACTGGGATAAAAATGGAATGGTGAACTTCAAGGAGTTCCTTTTTGCATTCACTCGATGGATTGGAATTGACGATCTTGAGGATGAAGATGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

226

Amino Acids

25.7

Weight (kDa)

4.51

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 58 - 115 1.7e-10 EF-hand domain pair
EF-hand_1 PF00036 58 - 85 3.7e-06 EF hand domain
EF-hand_6 PF13405 59 - 84 8.7e-06 EF-hand domain
EF-hand_8 PF13833 69 - 117 1.1e-06 EF-hand domain pair
EF-hand_7 PF13499 147 - 211 5e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 30
AflII CTTAAG 1 cut(s) 163
AgsI TTSAA 7 cut(s) 77, 124, 139, 207, 235, 575, 613
AluBI AGCT 5 cut(s) 58, 80, 98, 162, 242
AluI AGCT 5 cut(s) 58, 80, 98, 162, 242
Alw26I GTCTC 1 cut(s) 265
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 3 cut(s) 85, 95, 296
AseI ATTAAT 2 cut(s) 309, 519
Asp700I GAANNNNTTC 2 cut(s) 119, 507
AsuHPI GGTGA 1 cut(s) 616
AsuII TTCGAA 1 cut(s) 438
BbvI GCAGC 3 cut(s) 97, 107, 308
BccI CCATC 3 cut(s) 476, 574, 633
BclI TGATCA 2 cut(s) 220, 379
BcoDI GTCTC 1 cut(s) 265
BfmI CTRYAG 1 cut(s) 9
BfrI CTTAAG 1 cut(s) 163
BisI GCNGC 3 cut(s) 86, 96, 297
BlsI GCNGC 3 cut(s) 87, 97, 298
BmrI ACTGGG 1 cut(s) 595
BmsI GCATC 2 cut(s) 222, 442
BmuI ACTGGG 1 cut(s) 595
Bpu14I TTCGAA 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 680
Bsa29I ATCGAT 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 419
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 258, 590
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 4 cut(s) 382, 457, 487, 670
BseLI CCNNNNNNNGG 1 cut(s) 30
BseNI ACTGG 2 cut(s) 258, 590
BseRI GAGGAG 1 cut(s) 535
BseXI GCAGC 3 cut(s) 97, 107, 308
BseYI CCCAGC 1 cut(s) 389
BshFI GGCC 1 cut(s) 23
BshVI ATCGAT 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 1 cut(s) 265
BsmI GAATGC 3 cut(s) 391, 420, 629
BsnI GGCC 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 438
Bsp143I GATC 4 cut(s) 220, 286, 379, 655
BspANI GGCC 1 cut(s) 23
BspDI ATCGAT 1 cut(s) 282
BspHI TCATGA 2 cut(s) 111, 223
BspQI GCTCTTC 1 cut(s) 85
BspT104I TTCGAA 1 cut(s) 438
BspTI CTTAAG 1 cut(s) 163
BsrI ACTGG 2 cut(s) 258, 590
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 4 cut(s) 220, 286, 379, 655
BssT1I CCWWGG 1 cut(s) 419
Bst6I CTCTTC 2 cut(s) 85, 570
BstAFI CTTAAG 1 cut(s) 163
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 301
BstF5I GGATG 4 cut(s) 382, 457, 487, 670
BstKTI GATC 4 cut(s) 223, 289, 382, 658
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 4 cut(s) 220, 286, 379, 655
BstNSI RCATGY 1 cut(s) 303
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 3 cut(s) 97, 107, 308
Bsu15I ATCGAT 1 cut(s) 282
BsuRI GGCC 1 cut(s) 23
BsuTUI ATCGAT 1 cut(s) 282
BtsCI GGATG 4 cut(s) 382, 457, 487, 670
Cac8I GCNNGC 1 cut(s) 301
CciI TCATGA 2 cut(s) 111, 223
ClaI ATCGAT 1 cut(s) 282
CseI GACGC 1 cut(s) 346
CviAII CATG 4 cut(s) 112, 224, 300, 562
DpnI GATC 4 cut(s) 222, 288, 381, 657
DpnII GATC 4 cut(s) 220, 286, 379, 655
Eam1104I CTCTTC 2 cut(s) 85, 570
EarI CTCTTC 2 cut(s) 85, 570
Eco130I CCWWGG 1 cut(s) 419
Eco32I GATATC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 392
EcoRV GATATC 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 419
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 4 cut(s) 115, 227, 303, 565
FaiI YATR 9 cut(s) 83, 113, 131, 225, 301, 320, 410, 489, 563
FatI CATG 4 cut(s) 111, 223, 299, 561
FbaI TGATCA 2 cut(s) 220, 379
Fnu4HI GCNGC 3 cut(s) 86, 96, 297
FokI GGATG 4 cut(s) 389, 464, 494, 677
Fsp4HI GCNGC 3 cut(s) 86, 96, 297
GluI GCNGC 3 cut(s) 86, 96, 297
GsaI CCCAGC 1 cut(s) 393
HaeIII GGCC 1 cut(s) 23
HgaI GACGC 1 cut(s) 346
Hin1II CATG 4 cut(s) 115, 227, 303, 565
HinfI GANTC 3 cut(s) 34, 203, 324
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 2 cut(s) 267, 607
Hpy188I TCNGA 3 cut(s) 49, 372, 384
Hpy188III TCNNGA 4 cut(s) 112, 136, 224, 659
Hpy8I GTNNAC 2 cut(s) 267, 607
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 2 cut(s) 367, 445
HpyCH4V TGCA 5 cut(s) 85, 174, 296, 455, 629
Hsp92II CATG 4 cut(s) 115, 227, 303, 565
Ksp22I TGATCA 2 cut(s) 220, 379
Kzo9I GATC 4 cut(s) 220, 286, 379, 655
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 1 cut(s) 548
LpnPI CCDG 7 cut(s) 44, 239, 375, 410, 519, 534, 571
Lsp1109I GCAGC 3 cut(s) 97, 107, 308
LweI GCATC 2 cut(s) 222, 442
MalI GATC 4 cut(s) 222, 288, 381, 657
MboI GATC 4 cut(s) 220, 286, 379, 655
MboII GAAGA 4 cut(s) 102, 359, 587, 680
MluCI AATT 4 cut(s) 169, 230, 328, 648
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 5 cut(s) 193, 268, 307, 556, 655
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 2 cut(s) 119, 507
MseI TTAA 4 cut(s) 164, 309, 333, 519
MspCI CTTAAG 1 cut(s) 163
Mva1269I GAATGC 3 cut(s) 391, 420, 629
NdeII GATC 4 cut(s) 220, 286, 379, 655
NlaIII CATG 4 cut(s) 115, 227, 303, 565
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 303
NspV TTCGAA 1 cut(s) 438
PaeI GCATGC 1 cut(s) 303
PagI TCATGA 2 cut(s) 111, 223
PciSI GCTCTTC 1 cut(s) 85
PctI GAATGC 3 cut(s) 391, 420, 629
PdmI GAANNNNTTC 2 cut(s) 119, 507
PfeI GAWTC 2 cut(s) 203, 324
PkrI GCNGC 3 cut(s) 87, 97, 298
PleI GAGTC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 42
PshBI ATTAAT 2 cut(s) 309, 519
PspFI CCCAGC 1 cut(s) 389
SapI GCTCTTC 1 cut(s) 85
SaqAI TTAA 4 cut(s) 164, 309, 333, 519
SatI GCNGC 3 cut(s) 86, 96, 297
Sau3AI GATC 4 cut(s) 220, 286, 379, 655
SchI GAGTC 1 cut(s) 43
SetI ASST 6 cut(s) 60, 82, 100, 164, 244, 437
SfaNI GCATC 2 cut(s) 222, 442
SfcI CTRYAG 1 cut(s) 9
SfuI TTCGAA 1 cut(s) 438
SmlI CTYRAG 2 cut(s) 163, 659
SmoI CTYRAG 2 cut(s) 163, 659
SphI GCATGC 1 cut(s) 303
Sse9I AATT 4 cut(s) 169, 230, 328, 648
StyI CCWWGG 1 cut(s) 419
TaqI TCGA 4 cut(s) 41, 282, 438, 637
TasI AATT 4 cut(s) 169, 230, 328, 648
TfiI GAWTC 2 cut(s) 203, 324
Tru1I TTAA 4 cut(s) 164, 309, 333, 519
Tru9I TTAA 4 cut(s) 164, 309, 333, 519
TseI GCWGC 3 cut(s) 85, 95, 296
TspDTI ATGAA 6 cut(s) 128, 243, 331, 537, 578, 681
Vha464I CTTAAG 1 cut(s) 163
VspI ATTAAT 2 cut(s) 309, 519
XceI RCATGY 1 cut(s) 303
XmnI GAANNNNTTC 2 cut(s) 119, 507
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.