Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0004995

Physical Location & Seq

Genomic Coordinates Reverse (-)

2541680 .. 2542848

Gene Structure 1169 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g03220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 684 bp

ATGGGGGGCGTAGTGGGAAAGAGCGAAAGTTCTAGGAAGTTTTCGATACATGAAACGAAGCTAGAGGCGAAGATGGTTGAAGCTATGCAGCGCAGAGCAGCGAAAGGAAGTGTTATGAAATCATTTAACAGCCTCATCTTGAAGTTCCCAAAGATCGACGAGAGCTTAAGAAATTGCAAGGCTATTTTCGAGCAATTTGATGAGGATTCGAATGGTATGATCGATCAAGAAGAGATGAAAAAATGTTTCTCTAAACTGGAAGTCTCTTTTACAGACGAGGAGATCATTGATCTCTTTGAGGCATGCGATATTAACGAGGATATGGGAATCAAGTTTAACGAGTTCATCGTTCTTATTTGCCTCGTTCATCTTCTTAAGGATGATAACAAAGCCATTCATACAAAATTGCAACTGGGGATGCCGGAACTGGAGGCCACGTTTGAAACACTGGTAGACGCGTTTGTGTTCTTGGACAAGAACAAGGATGGTTATGTCAGCAAGAGTGAAATGGTCGAGGCCATCAACGAAACTACAACAGGAGAACGTTCCTCCGGCCGCATAGCCATGAAAAGATTTGAAGAGATGGACTGGGATAAAAACGGAATGGTGAACTTCAAGGAGTTTCTGTTTGCGTTTACTCGATGGATTGGAATCGACGATCTTGAGGACGAGGATGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

228

Amino Acids

26.11

Weight (kDa)

4.66

Isoelectric Point (pI)

42.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	59 - 116	3.1e-09	EF-hand domain pair
EF-hand_7	PF13499	148 - 212	1.6e-08	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004995)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26470 AT4G26470 AT4G26470
fragaria_vesca	FvH4_3g41720 FvH4_3g41720 FvH4_3g41720 FvH4_3g41720
malus_domestica	MD03G1041500.v1.1 MD11G1042400.v1.1
prunus_persica	Prupe.6G033600_v2.0.a1 Prupe.6G033600_v2.0.a1
pyrus_communis	pycom03g03220 pycom11g03650
rosa_chinensis	RchiOBHm_Chr5g0074811
rosa_laevigata	RLG00000036507
rosa_multiflora	Rmu_co8378831.1_g000001 Rmu_sc0000909.1_g000003 Rmu_sc0005365.1_g000009
rosa_roxburghii	Rroxscaffold_1G00006760
rosa_rugosa	Rorug05G0434000 Rorug05G0434100 Rorug05G0434100 Rorug05G0434200 Rorug05G0434300 Rorug05G0434400
rosa_samantha	Rh5AG491900 Rh5BG513300 Rh5CG537000 Rh5DG525400
rosa_wichuraiana	Rw5G045570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	453
AccII	CGCG	1 cut(s)	458
AciI	CCGC	1 cut(s)	556
AclI	AACGTT	1 cut(s)	544
AcoI	YGGCCR	1 cut(s)	553
AflII	CTTAAG	2 cut(s)	166, 374
AflIII	ACRYGT	1 cut(s)	456
AgsI	TTSAA	5 cut(s)	80, 142, 443, 578, 616
AluBI	AGCT	3 cut(s)	61, 83, 165
AluI	AGCT	3 cut(s)	61, 83, 165
Alw26I	GTCTC	1 cut(s)	268
AoxI	GGCC	3 cut(s)	432, 516, 553
ApeKI	GCWGC	2 cut(s)	88, 98
AspLEI	GCGC	1 cut(s)	93
AsuHPI	GGTGA	1 cut(s)	619
AsuII	TTCGAA	1 cut(s)	209
BbvI	GCAGC	2 cut(s)	100, 110
BccI	CCATC	5 cut(s)	67, 479, 527, 577, 636
BcoDI	GTCTC	1 cut(s)	268
BfaI	CTAG	2 cut(s)	33, 62
BfrI	CTTAAG	2 cut(s)	166, 374
BisI	GCNGC	3 cut(s)	89, 99, 556
BlsI	GCNGC	3 cut(s)	90, 100, 557
BmrI	ACTGGG	2 cut(s)	422, 598
BmsI	GCATC	1 cut(s)	408
BmuI	ACTGGG	2 cut(s)	422, 598
BpmI	CTGGAG	1 cut(s)	449
Bpu14I	TTCGAA	1 cut(s)	209
BpuEI	CTTGAG	1 cut(s)	683
Bsa29I	ATCGAT	1 cut(s)	222
BsaBI	GATNNNNATC	1 cut(s)	650
BsaXI	ACNNNNNCTCC	4 cut(s)	422, 452, 611, 641
Bse1I	ACTGG	5 cut(s)	261, 417, 432, 453, 593
Bse8I	GATNNNNATC	1 cut(s)	650
BseCI	ATCGAT	1 cut(s)	222
BseGI	GGATG	4 cut(s)	385, 423, 490, 679
BseJI	GATNNNNATC	1 cut(s)	650
BseNI	ACTGG	5 cut(s)	261, 417, 432, 453, 593
BseRI	GAGGAG	1 cut(s)	293
BseX3I	CGGCCG	1 cut(s)	553
BseXI	GCAGC	2 cut(s)	100, 110
Bsh1236I	CGCG	1 cut(s)	458
Bsh1285I	CGRYCG	1 cut(s)	556
BshFI	GGCC	3 cut(s)	434, 518, 555
BshVI	ATCGAT	1 cut(s)	222
BsiEI	CGRYCG	1 cut(s)	556
BsiSI	CCGG	2 cut(s)	422, 552
BsmAI	GTCTC	1 cut(s)	268
BsnI	GGCC	3 cut(s)	434, 518, 555
Bsp119I	TTCGAA	1 cut(s)	209
Bsp143I	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
BspACI	CCGC	1 cut(s)	556
BspANI	GGCC	3 cut(s)	434, 518, 555
BspDI	ATCGAT	1 cut(s)	222
BspFNI	CGCG	1 cut(s)	458
BspT104I	TTCGAA	1 cut(s)	209
BspTI	CTTAAG	2 cut(s)	166, 374
BsrI	ACTGG	5 cut(s)	261, 417, 432, 453, 593
BssMI	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
Bst6I	CTCTTC	2 cut(s)	225, 573
BstAFI	CTTAAG	2 cut(s)	166, 374
BstBI	TTCGAA	1 cut(s)	209
BstC8I	GCNNGC	1 cut(s)	304
BstF5I	GGATG	4 cut(s)	385, 423, 490, 679
BstFNI	CGCG	1 cut(s)	458
BstHHI	GCGC	1 cut(s)	93
BstKTI	GATC	6 cut(s)	156, 222, 226, 285, 292, 661
BstMAI	GTCTC	1 cut(s)	268
BstMBI	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
BstMCI	CGRYCG	1 cut(s)	556
BstNSI	RCATGY	1 cut(s)	306
BstUI	CGCG	1 cut(s)	458
BstV1I	GCAGC	2 cut(s)	100, 110
BstZI	CGGCCG	1 cut(s)	553
Bsu15I	ATCGAT	1 cut(s)	222
BsuRI	GGCC	3 cut(s)	434, 518, 555
BsuTUI	ATCGAT	1 cut(s)	222
BtsCI	GGATG	4 cut(s)	385, 423, 490, 679
BtsIMutI	CAGTG	1 cut(s)	446
Cac8I	GCNNGC	1 cut(s)	304
CfoI	GCGC	1 cut(s)	93
ClaI	ATCGAT	1 cut(s)	222
CseI	GACGC	1 cut(s)	464
CviAII	CATG	3 cut(s)	50, 303, 565
DpnI	GATC	6 cut(s)	155, 221, 225, 284, 291, 660
DpnII	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
EaeI	YGGCCR	1 cut(s)	553
EagI	CGGCCG	1 cut(s)	553
Eam1104I	CTCTTC	2 cut(s)	225, 573
EarI	CTCTTC	2 cut(s)	225, 573
EclXI	CGGCCG	1 cut(s)	553
Eco52I	CGGCCG	1 cut(s)	553
FaeI	CATG	3 cut(s)	53, 306, 568
FatI	CATG	3 cut(s)	49, 302, 564
FblI	GTMKAC	1 cut(s)	453
Fnu4HI	GCNGC	3 cut(s)	89, 99, 556
FokI	GGATG	3 cut(s)	392, 430, 497
Fsp4HI	GCNGC	3 cut(s)	89, 99, 556
FspBI	CTAG	2 cut(s)	33, 62
GlaI	GCGC	1 cut(s)	92
GluI	GCNGC	3 cut(s)	89, 99, 556
GsuI	CTGGAG	1 cut(s)	449
HaeIII	GGCC	3 cut(s)	434, 518, 555
HapII	CCGG	2 cut(s)	422, 552
HgaI	GACGC	1 cut(s)	464
HhaI	GCGC	1 cut(s)	93
Hin1II	CATG	3 cut(s)	53, 306, 568
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HinfI	GANTC	3 cut(s)	206, 327, 651
HpaII	CCGG	2 cut(s)	422, 552
HphI	GGTGA	1 cut(s)	619
Hpy166II	GTNNAC	3 cut(s)	454, 610, 636
Hpy188III	TCNNGA	3 cut(s)	139, 227, 662
Hpy8I	GTNNAC	3 cut(s)	454, 610, 636
Hpy99I	CGWCG	2 cut(s)	161, 659
HpyCH4IV	ACGT	2 cut(s)	437, 544
HpyCH4V	TGCA	3 cut(s)	88, 177, 409
HpySE526I	ACGT	2 cut(s)	437, 544
Hsp92II	CATG	3 cut(s)	53, 306, 568
HspAI	GCGC	1 cut(s)	91
Kzo9I	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
LpnPI	CCDG	8 cut(s)	242, 398, 413, 434, 435, 522, 565, 574
Lsp1109I	GCAGC	2 cut(s)	100, 110
LweI	GCATC	1 cut(s)	408
MaeI	CTAG	2 cut(s)	33, 62
MaeII	ACGT	2 cut(s)	437, 544
MalI	GATC	6 cut(s)	155, 221, 225, 284, 291, 660
MboI	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
MboII	GAAGA	4 cut(s)	82, 242, 362, 590
MluCI	AATT	3 cut(s)	172, 194, 404
MluI	ACGCGT	1 cut(s)	456
MseI	TTAA	5 cut(s)	126, 167, 312, 336, 375
MslI	CAYNNNNRTG	1 cut(s)	563
MspCI	CTTAAG	2 cut(s)	166, 374
MspI	CCGG	2 cut(s)	422, 552
MvnI	CGCG	1 cut(s)	458
NdeII	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
NlaIII	CATG	3 cut(s)	53, 306, 568
NspI	RCATGY	1 cut(s)	306
NspV	TTCGAA	1 cut(s)	209
PaeI	GCATGC	1 cut(s)	306
PcsI	WCGNNNNNNNCGW	1 cut(s)	345
PfeI	GAWTC	3 cut(s)	206, 327, 651
PkrI	GCNGC	3 cut(s)	90, 100, 557
Psp1406I	AACGTT	1 cut(s)	544
RseI	CAYNNNNRTG	1 cut(s)	563
SaqAI	TTAA	5 cut(s)	126, 167, 312, 336, 375
SatI	GCNGC	3 cut(s)	89, 99, 556
Sau3AI	GATC	6 cut(s)	153, 219, 223, 282, 289, 658
SetI	ASST	5 cut(s)	63, 85, 167, 440, 547
SfaNI	GCATC	1 cut(s)	408
SfuI	TTCGAA	1 cut(s)	209
SmiMI	CAYNNNNRTG	1 cut(s)	563
SmlI	CTYRAG	3 cut(s)	166, 374, 662
SmoI	CTYRAG	3 cut(s)	166, 374, 662
SphI	GCATGC	1 cut(s)	306
Sse9I	AATT	3 cut(s)	172, 194, 404
SsiI	CCGC	1 cut(s)	556
SspMI	CTAG	2 cut(s)	33, 62
TaiI	ACGT	2 cut(s)	440, 547
TaqI	TCGA	8 cut(s)	44, 156, 189, 209, 222, 513, 640, 654
TasI	AATT	3 cut(s)	172, 194, 404
TauI	GCSGC	1 cut(s)	558
TfiI	GAWTC	3 cut(s)	206, 327, 651
Tru1I	TTAA	5 cut(s)	126, 167, 312, 336, 375
Tru9I	TTAA	5 cut(s)	126, 167, 312, 336, 375
TscAI	CASTG	1 cut(s)	453
TseI	GCWGC	2 cut(s)	88, 98
TspDTI	ATGAA	7 cut(s)	66, 131, 251, 334, 356, 386, 581
TspGWI	ACGGA	1 cut(s)	615
TspRI	CASTG	1 cut(s)	453
Vha464I	CTTAAG	2 cut(s)	166, 374
XceI	RCATGY	1 cut(s)	306
XmiI	GTMKAC	1 cut(s)	453
XspI	CTAG	2 cut(s)	33, 62

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.