Rorug05G0434100

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004995
Physical Location & Seq
Forward (+)
60299068 .. 60304435
5368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0434100.1

Sequence Viewer

Length: 945 bp
ATGTCAAGAAAGAAATTGGAATCTGAAGCAAAGTTTGACACTTTCCCTGAGGAAATCCGTCAGGATATCTTTCTTAGACTACCCATTAAGTCTTTGATCAGGTGCACAGCTGTGCGCAAGGCATGGAGATCAGTCATAAGAAACCCAAGCTTTATTCGCACCCACCTCCGCCGGACACACAATTCCAATGAACAGAATGACACTCAACTCTTATTGATCCATGGTTTTGCCAAAAAGGCTTTTAGCTTTGATTACAGTGATAGTGAGGATGACATACAGGAGCTTTATTCTTTGCATTATGATAACCCTGCGTTTGACGAGCACTGCAAGGTTGAAGTTCCAACTATTGAGTTGGCGAATGCATGTTTCCGTGTTGTTGGCACTTGTAATGGGCTGGTTTGCCTTGCAGATGATTTAGGGTTATATATGAACACCTACATATTATCAAATCCATCTATAAGAAAGTCTGTGGCCCTTCCCGAGCCTAATGTTACATATTGCACTCATGGTGGGCATGTAGCTTCTATTGGGTTTGGTTTTGATGCTATGACCAACGACTACAAGGTTGTGAGGCTTGTCACTCTTTTGGATGAAGATTCACAAGATGCCCTTTGTCGAACTGTAGTTGAAGTTTTTTCACTAGCCACAGGCTCCTGGAGCATGCTTCCTGATGTTGCTCCTCGGTTTGAAATGGATGCATGCACTCCCCAGGCTTTAGTTAATGGGGCTCTTCATTGGCACGCAAAGCGGCGGAAAGAAGATGATTTTTACTATTTCATTTTGATATTTGATGTCAGCAGGGGTTTATTTCGTGAGACCATGATGCCAGAGAGTTTGAAATGCGATCCGGAATTAACATTGCAGTTGTCTGTTTCTGGAGATGGGAAGTCCATTGCTCTGTCCAGCATGTATTCTAGTCATTTTTGTGCTGATCCTTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

314

Amino Acids

35.68

Weight (kDa)

5.59

Isoelectric Point (pI)

55.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 15 - 55 3.7e-06 F-box-like
FBA_3 PF08268 105 - 279 1.1e-12 F-box associated beta propeller domain
FBA_1 PF07734 120 - 301 7.3e-19 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 124 - 265 3.1e-09 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 116
AccIII TCCGGA 1 cut(s) 847
AciI CCGC 3 cut(s) 169, 748, 751
AclWI GGATC 3 cut(s) 211, 839, 926
AcuI CTGAAG 1 cut(s) 45
AgsI TTSAA 4 cut(s) 335, 629, 689, 838
AjnI CCWGG 2 cut(s) 653, 708
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 5 cut(s) 110, 150, 246, 283, 521
AluI AGCT 5 cut(s) 110, 150, 246, 283, 521
Alw21I GWGCWC 2 cut(s) 107, 324
Alw26I GTCTC 1 cut(s) 809
Alw44I GTGCAC 1 cut(s) 103
AlwI GGATC 3 cut(s) 211, 839, 926
Ama87I CYCGRG 1 cut(s) 479
Aor13HI TCCGGA 1 cut(s) 847
AoxI GGCC 1 cut(s) 471
ApaLI GTGCAC 1 cut(s) 103
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 472
AvaI CYCGRG 1 cut(s) 479
AxyI CCTNAGG 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 730
Bbv12I GWGCWC 2 cut(s) 107, 324
BccI CCATC 2 cut(s) 460, 875
BciT130I CCWGG 2 cut(s) 655, 710
BclI TGATCA 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 809
BfaI CTAG 2 cut(s) 641, 915
BfmI CTRYAG 1 cut(s) 621
BglI GCCNNNNNGGC 1 cut(s) 236
BisI GCNGC 1 cut(s) 749
BlsI GCNGC 1 cut(s) 750
Bme1390I CCNGG 2 cut(s) 655, 710
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 472
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 2 cut(s) 655, 710
BmsI GCATC 4 cut(s) 532, 595, 685, 813
BpmI CTGGAG 2 cut(s) 676, 897
BsaI GGTCTC 1 cut(s) 809
BsaJI CCNNGG 3 cut(s) 220, 680, 708
BsaWI WCCGGW 1 cut(s) 847
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 2 cut(s) 857, 891
BseAI TCCGGA 1 cut(s) 847
BseBI CCWGG 2 cut(s) 655, 710
BseDI CCNNGG 3 cut(s) 220, 680, 708
BseGI GGATG 3 cut(s) 274, 595, 700
BseMI GCAATG 2 cut(s) 857, 891
BseMII CTCAG 1 cut(s) 39
BseRI GAGGAG 1 cut(s) 669
BseSI GKGCMC 1 cut(s) 107
BshFI GGCC 1 cut(s) 473
BsiHKAI GWGCWC 2 cut(s) 107, 324
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 2 cut(s) 172, 848
BsmAI GTCTC 1 cut(s) 809
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 473
Bso31I GGTCTC 1 cut(s) 809
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 107, 324, 730
Bsp13I TCCGGA 1 cut(s) 847
Bsp143I GATC 5 cut(s) 96, 128, 216, 844, 931
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 3 cut(s) 169, 748, 751
BspANI GGCC 1 cut(s) 473
BspCNI CTCAG 1 cut(s) 40
BspEI TCCGGA 1 cut(s) 847
BspLI GGNNCC 1 cut(s) 652
BspPI GGATC 3 cut(s) 211, 839, 926
BspQI GCTCTTC 1 cut(s) 735
BspTNI GGTCTC 1 cut(s) 809
BsrDI GCAATG 2 cut(s) 857, 891
BssECI CCNNGG 3 cut(s) 220, 680, 708
BssMI GATC 5 cut(s) 96, 128, 216, 844, 931
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 2 cut(s) 655, 710
Bst4CI ACNGT 2 cut(s) 257, 622
Bst6I CTCTTC 1 cut(s) 735
BstC8I GCNNGC 3 cut(s) 662, 700, 741
BstDEI CTNAG 2 cut(s) 48, 74
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 3 cut(s) 274, 595, 700
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 5 cut(s) 99, 131, 219, 847, 934
BstMAI GTCTC 1 cut(s) 809
BstMBI GATC 5 cut(s) 96, 128, 216, 844, 931
BstMWI GCNNNNNNNGC 4 cut(s) 156, 236, 657, 745
BstNI CCWGG 2 cut(s) 655, 710
BstNSI RCATGY 5 cut(s) 366, 518, 664, 702, 910
BstSCI CCNGG 2 cut(s) 653, 708
BstSFI CTRYAG 1 cut(s) 621
BstSLI GKGCMC 1 cut(s) 107
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 1 cut(s) 473
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 3 cut(s) 274, 595, 700
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 2 cut(s) 262, 322
Cac8I GCNNGC 3 cut(s) 662, 700, 741
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 472
CviAII CATG 9 cut(s) 123, 221, 363, 506, 515, 661, 699, 820, 907
DdeI CTNAG 2 cut(s) 48, 74
DpnI GATC 5 cut(s) 98, 130, 218, 846, 933
DpnII GATC 5 cut(s) 96, 128, 216, 844, 931
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
EciI GGCGGA 2 cut(s) 158, 766
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 730
Eco31I GGTCTC 1 cut(s) 809
Eco32I GATATC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 45
Eco81I CCTNAGG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 479
EcoRII CCWGG 2 cut(s) 653, 708
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 220
EcoT22I ATGCAT 2 cut(s) 364, 700
EcoT38I GRGCYC 1 cut(s) 730
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 9 cut(s) 126, 224, 366, 509, 518, 664, 702, 823, 910
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FatI CATG 9 cut(s) 122, 220, 362, 505, 514, 660, 698, 819, 906
FbaI TGATCA 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 749
FokI GGATG 3 cut(s) 281, 602, 707
FriOI GRGCYC 1 cut(s) 730
Fsp4HI GCNGC 1 cut(s) 749
FspBI CTAG 2 cut(s) 641, 915
FspI TGCGCA 1 cut(s) 116
GlaI GCGC 1 cut(s) 116
GluI GCNGC 1 cut(s) 749
GsuI CTGGAG 2 cut(s) 676, 897
HaeIII GGCC 1 cut(s) 473
HapII CCGG 2 cut(s) 172, 848
HhaI GCGC 1 cut(s) 117
Hin1II CATG 9 cut(s) 126, 224, 366, 509, 518, 664, 702, 823, 910
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 2 cut(s) 20, 596
HpaII CCGG 2 cut(s) 172, 848
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 7 cut(s) 6, 62, 479, 668, 812, 848, 876
Hpy8I GTNNAC 1 cut(s) 105
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 2 cut(s) 257, 622
HpyCH4V TGCA 9 cut(s) 105, 295, 327, 362, 407, 501, 698, 702, 862
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 236, 657, 745
HpyF3I CTNAG 2 cut(s) 48, 74
Hsp92II CATG 9 cut(s) 126, 224, 366, 509, 518, 664, 702, 823, 910
HspAI GCGC 1 cut(s) 115
Kpn2I TCCGGA 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 5 cut(s) 96, 128, 216, 844, 931
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 4 cut(s) 280, 656, 657, 682
LweI GCATC 4 cut(s) 532, 595, 685, 813
MaeI CTAG 2 cut(s) 641, 915
MaeIII GTNAC 2 cut(s) 490, 577
MalI GATC 5 cut(s) 98, 130, 218, 846, 933
MboI GATC 5 cut(s) 96, 128, 216, 844, 931
MboII GAAGA 3 cut(s) 605, 722, 770
MhlI GDGCHC 3 cut(s) 107, 324, 730
MluCI AATT 3 cut(s) 14, 181, 851
MmeI TCCRAC 1 cut(s) 365
MnlI CCTC 5 cut(s) 43, 176, 259, 564, 690
Mph1103I ATGCAT 2 cut(s) 364, 700
MroI TCCGGA 1 cut(s) 847
MseI TTAA 3 cut(s) 87, 720, 854
MslI CAYNNNNRTG 2 cut(s) 110, 924
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 2 cut(s) 172, 848
MspR9I CCNGG 2 cut(s) 655, 710
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 2 cut(s) 655, 710
MwoI GCNNNNNNNGC 4 cut(s) 156, 236, 657, 745
NcoI CCATGG 1 cut(s) 220
NdeII GATC 5 cut(s) 96, 128, 216, 844, 931
NlaIII CATG 9 cut(s) 126, 224, 366, 509, 518, 664, 702, 823, 910
NlaIV GGNNCC 1 cut(s) 652
NmuCI GTSAC 1 cut(s) 577
NsbI TGCGCA 1 cut(s) 116
NsiI ATGCAT 2 cut(s) 364, 700
NspI RCATGY 5 cut(s) 366, 518, 664, 702, 910
OliI CACNNNNGTG 1 cut(s) 110
PaeI GCATGC 2 cut(s) 664, 702
PciSI GCTCTTC 1 cut(s) 735
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 20, 596
PfoI TCCNGGA 1 cut(s) 653
PkrI GCNGC 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 653, 708
PspGI CCWGG 2 cut(s) 653, 708
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 110
RseI CAYNNNNRTG 2 cut(s) 110, 924
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 3 cut(s) 87, 720, 854
SatI GCNGC 1 cut(s) 749
Sau3AI GATC 5 cut(s) 96, 128, 216, 844, 931
Sau96I GGNCC 1 cut(s) 472
ScrFI CCNGG 2 cut(s) 655, 710
SduI GDGCHC 3 cut(s) 107, 324, 730
SfaNI GCATC 4 cut(s) 532, 595, 685, 813
SfcI CTRYAG 1 cut(s) 621
SmiMI CAYNNNNRTG 2 cut(s) 110, 924
SphI GCATGC 2 cut(s) 664, 702
Sse9I AATT 3 cut(s) 14, 181, 851
SsiI CCGC 3 cut(s) 169, 748, 751
SspMI CTAG 2 cut(s) 641, 915
StyD4I CCNGG 2 cut(s) 653, 708
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 2 cut(s) 257, 622
TaqI TCGA 1 cut(s) 616
TasI AATT 3 cut(s) 14, 181, 851
TauI GCSGC 1 cut(s) 751
TfiI GAWTC 2 cut(s) 20, 596
Tru1I TTAA 3 cut(s) 87, 720, 854
Tru9I TTAA 3 cut(s) 87, 720, 854
TscAI CASTG 2 cut(s) 262, 329
TseFI GTSAC 1 cut(s) 577
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 5 cut(s) 204, 443, 606, 722, 766
TspGWI ACGGA 2 cut(s) 47, 359
TspRI CASTG 2 cut(s) 262, 329
VneI GTGCAC 1 cut(s) 103
XceI RCATGY 5 cut(s) 366, 518, 664, 702, 910
XspI CTAG 2 cut(s) 641, 915
Zsp2I ATGCAT 2 cut(s) 364, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.