Rorug05G0434300

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004995
Physical Location & Seq
Forward (+)
60317246 .. 60318367
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0434300.1

Sequence Viewer

Length: 1122 bp
ATGGCTTCGTCGTCCTGTACACCCCATGATATACCTTACCATGATGAGGGTATCATAGAGCCTATGTCAATTTGCTTTGAAGATACTGCTGACTTCCTGGACTTGGAATCTGGTATAAGCCTACTCGGAATTCTTATTGCCGATGAGGAACCCGGTCCGGGAGGAATCAAAGCCAATTTGATGGGCATGTGGAAAACTTTGGGCCAGATCCGGATAATCCGAGTCAAGAAAAACACTTACTCTCTAACTATGGGTTCAGAGAAACTTGCAAATAAATTGATCAATGATTGCCCCTGGAATGTAAGAGGGTACTGCTTTTCTGTTCGTCATTGGCCCCATTACCACTCCATTGATGACATCGATACAACTCGTGTCACTTTCTGGATCCAAGCACACGGTATCCCCCTCGACCAGATGACTATGGTAAATGGAAGAAAGCTGGGAGAAAAGCTGGGTGCTATCCTTGAAGTCGAAGACCCAATAGCTGTTGGCAACAGAGGCTACCTCAGACTGCGAGTAGATTTTGATGCCAGCAGACCCCTAGCTACTTTCATCCAACTCCCACGAAGAACAGCTAATGTGACAAAGATTTGGTTCCGGTATGAGGGTCTCAAAAATTTCTGCTACCACTGTGGGAGATTAGGTCATTCCAACACCTCCTGCAGATACGAGGTGAACCCAATACTCTTAAGTCTAGGAGTGGTGTATGATAGATCCCTAATTGCAGAACCTCCACAAAAACAGATGAACACCCAAGCCTCATTCCCACTGGATTTTCCATATGCTCCGACTTCCGGCATCTTTCGCCGGAGGACTGGTGATACCGAGGTAATTTCAAACTTGAATTCTGGTAGAAAGGGACTGATCGGTGATAATAGTAGTGAGCAGGCACACCTTTCAACTTTTGATGGCGACAGCTCCTCCCATGCCAGTAAGAGAAAAAGGGATAGCACACTAACAACTGTTGTAGTACCTAAACCAAGGTATCAGTGTGGGGGTCCTAGCATGTTCGACTCTGATGCACATGGGGCAATGTACAGGAACGGTAGCGTTACAAGGGCATTTAATGGGCTAAATTTACACCAGAGACCTTGGGCTGACCCAGAGGTAATACCCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

373

Amino Acids

41.67

Weight (kDa)

7.64

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 175 - 223 4.3e-10 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 210
AclWI GGATC 4 cut(s) 202, 379, 392, 708
AcsI RAATTY 4 cut(s) 129, 616, 844, 1075
AfaI GTAC 4 cut(s) 19, 311, 972, 1037
AfiI CCNNNNNNNGG 5 cut(s) 46, 103, 158, 604, 794
AflII CTTAAG 1 cut(s) 688
AgsI TTSAA 5 cut(s) 80, 467, 837, 844, 900
AjnI CCWGG 2 cut(s) 96, 293
AluBI AGCT 6 cut(s) 439, 451, 485, 545, 575, 918
AluI AGCT 6 cut(s) 439, 451, 485, 545, 575, 918
Alw26I GTCTC 2 cut(s) 614, 1081
AlwI GGATC 4 cut(s) 202, 379, 392, 708
Aor13HI TCCGGA 1 cut(s) 210
AoxI GGCC 2 cut(s) 202, 332
ApoI RAATTY 4 cut(s) 129, 616, 844, 1075
AspS9I GGNCC 4 cut(s) 155, 202, 333, 998
AsuC2I CCSGG 2 cut(s) 153, 159
AsuHPI GGTGA 3 cut(s) 685, 830, 881
AvaII GGWCC 2 cut(s) 155, 998
BaeI ACNNNNGTAYC 4 cut(s) 813, 846, 968, 1001
BamHI GGATCC 1 cut(s) 384
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 480
BccI CCATC 2 cut(s) 175, 902
BcgI CGANNNNNNTGC 2 cut(s) 1001, 1035
BciT130I CCWGG 2 cut(s) 98, 295
BciVI GTATCC 1 cut(s) 410
BclI TGATCA 1 cut(s) 279
BcnI CCSGG 2 cut(s) 153, 159
BcoDI GTCTC 2 cut(s) 614, 1081
BfaI CTAG 4 cut(s) 542, 695, 1002, 1120
BfmI CTRYAG 1 cut(s) 661
BfrI CTTAAG 1 cut(s) 688
BfuI GTATCC 1 cut(s) 410
Bme1390I CCNGG 4 cut(s) 98, 153, 159, 295
Bme18I GGWCC 2 cut(s) 155, 998
BmgT120I GGNCC 4 cut(s) 155, 202, 333, 998
BmiI GGNNCC 5 cut(s) 150, 335, 386, 596, 999
BmrFI CCNGG 4 cut(s) 98, 153, 159, 295
BmsI GCATC 3 cut(s) 517, 807, 1009
BpiI GAAGAC 1 cut(s) 480
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpuMI CCSGG 2 cut(s) 153, 159
Bsa29I ATCGAT 1 cut(s) 360
BsaI GGTCTC 2 cut(s) 614, 1081
BsaJI CCNNGG 4 cut(s) 293, 825, 980, 1091
BsaWI WCCGGW 2 cut(s) 210, 597
BsaXI ACNNNNNCTCC 6 cut(s) 628, 658, 802, 832, 905, 935
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 103, 158, 604, 794
Bse1I ACTGG 3 cut(s) 774, 820, 930
Bse3DI GCAATG 1 cut(s) 1038
BseAI TCCGGA 1 cut(s) 210
BseBI CCWGG 2 cut(s) 98, 295
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 4 cut(s) 293, 825, 980, 1091
BseGI GGATG 1 cut(s) 552
BseLI CCNNNNNNNGG 5 cut(s) 46, 103, 158, 604, 794
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 1 cut(s) 520
BseNI ACTGG 3 cut(s) 774, 820, 930
BseRI GAGGAG 1 cut(s) 910
BseYI CCCAGC 2 cut(s) 439, 451
BshFI GGCC 2 cut(s) 204, 334
BshVI ATCGAT 1 cut(s) 360
BsiSI CCGG 6 cut(s) 153, 158, 211, 598, 795, 808
BslFI GGGAC 1 cut(s) 873
BslI CCNNNNNNNGG 5 cut(s) 46, 103, 158, 604, 794
BsmAI GTCTC 2 cut(s) 614, 1081
BsmFI GGGAC 1 cut(s) 873
BsnI GGCC 2 cut(s) 204, 334
Bso31I GGTCTC 2 cut(s) 614, 1081
Bsp13I TCCGGA 1 cut(s) 210
Bsp1407I TGTACA 2 cut(s) 17, 1035
Bsp143I GATC 5 cut(s) 207, 279, 384, 713, 864
BspANI GGCC 2 cut(s) 204, 334
BspCNI CTCAG 1 cut(s) 519
BspDI ATCGAT 1 cut(s) 360
BspEI TCCGGA 1 cut(s) 210
BspLI GGNNCC 5 cut(s) 150, 335, 386, 596, 999
BspMAI CTGCAG 1 cut(s) 665
BspPI GGATC 4 cut(s) 202, 379, 392, 708
BspTI CTTAAG 1 cut(s) 688
BspTNI GGTCTC 2 cut(s) 614, 1081
BsrDI GCAATG 1 cut(s) 1038
BsrGI TGTACA 2 cut(s) 17, 1035
BsrI ACTGG 3 cut(s) 774, 820, 930
BssECI CCNNGG 4 cut(s) 293, 825, 980, 1091
BssMI GATC 5 cut(s) 207, 279, 384, 713, 864
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 2 cut(s) 980, 1091
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 98, 295
Bst4CI ACNGT 4 cut(s) 398, 632, 964, 1046
BstAFI CTTAAG 1 cut(s) 688
BstAUI TGTACA 2 cut(s) 17, 1035
BstC8I GCNNGC 2 cut(s) 532, 888
BstDEI CTNAG 1 cut(s) 506
BstF5I GGATG 1 cut(s) 552
BstKTI GATC 5 cut(s) 210, 282, 387, 716, 867
BstMAI GTCTC 2 cut(s) 614, 1081
BstMBI GATC 5 cut(s) 207, 279, 384, 713, 864
BstMWI GCNNNNNNNGC 3 cut(s) 498, 804, 1028
BstNI CCWGG 2 cut(s) 98, 295
BstNSI RCATGY 2 cut(s) 190, 1009
BstSCI CCNGG 4 cut(s) 96, 151, 157, 293
BstSFI CTRYAG 1 cut(s) 661
BstV2I GAAGAC 1 cut(s) 480
BstX2I RGATCY 3 cut(s) 207, 384, 713
BstXI CCANNNNNNTGG 1 cut(s) 181
BstYI RGATCY 3 cut(s) 207, 384, 713
Bsu15I ATCGAT 1 cut(s) 360
BsuI GTATCC 1 cut(s) 410
BsuRI GGCC 2 cut(s) 204, 334
BsuTUI ATCGAT 1 cut(s) 360
BtsCI GGATG 1 cut(s) 552
BtsIMutI CAGTG 3 cut(s) 628, 767, 995
Cac8I GCNNGC 2 cut(s) 532, 888
Cfr13I GGNCC 4 cut(s) 155, 202, 333, 998
ClaI ATCGAT 1 cut(s) 360
CpoI CGGWCCG 1 cut(s) 155
Csp6I GTAC 4 cut(s) 18, 310, 971, 1036
CspI CGGWCCG 1 cut(s) 155
CviAII CATG 6 cut(s) 26, 41, 187, 926, 1006, 1025
CviQI GTAC 4 cut(s) 18, 310, 971, 1036
DdeI CTNAG 1 cut(s) 506
DpnI GATC 5 cut(s) 209, 281, 386, 715, 866
DpnII GATC 5 cut(s) 207, 279, 384, 713, 864
Eco130I CCWWGG 2 cut(s) 980, 1091
Eco31I GGTCTC 2 cut(s) 614, 1081
Eco47I GGWCC 2 cut(s) 155, 998
EcoO109I RGGNCCY 1 cut(s) 998
EcoRI GAATTC 2 cut(s) 129, 844
EcoRII CCWGG 2 cut(s) 96, 293
EcoT14I CCWWGG 2 cut(s) 980, 1091
ErhI CCWWGG 2 cut(s) 980, 1091
FaeI CATG 6 cut(s) 29, 44, 190, 929, 1009, 1028
FaqI GGGAC 1 cut(s) 873
FatI CATG 6 cut(s) 25, 40, 186, 925, 1005, 1024
FauNDI CATATG 1 cut(s) 781
FbaI TGATCA 1 cut(s) 279
FokI GGATG 1 cut(s) 539
FspBI CTAG 4 cut(s) 542, 695, 1002, 1120
GsaI CCCAGC 2 cut(s) 443, 455
HaeIII GGCC 2 cut(s) 204, 334
HapII CCGG 6 cut(s) 153, 158, 211, 598, 795, 808
Hin1II CATG 6 cut(s) 29, 44, 190, 929, 1009, 1028
HinfI GANTC 4 cut(s) 107, 165, 222, 1013
HpaII CCGG 6 cut(s) 153, 158, 211, 598, 795, 808
HphI GGTGA 3 cut(s) 685, 830, 881
Hpy166II GTNNAC 2 cut(s) 20, 676
Hpy188I TCNGA 6 cut(s) 128, 221, 259, 509, 789, 1018
Hpy188III TCNNGA 3 cut(s) 211, 226, 382
Hpy8I GTNNAC 2 cut(s) 20, 676
Hpy99I CGWCG 1 cut(s) 13
HpyCH4III ACNGT 4 cut(s) 398, 632, 964, 1046
HpyCH4V TGCA 4 cut(s) 269, 663, 725, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 498, 804, 1028
HpyF3I CTNAG 1 cut(s) 506
Hsp92II CATG 6 cut(s) 29, 44, 190, 929, 1009, 1028
Kpn2I TCCGGA 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 5 cut(s) 207, 279, 384, 713, 864
LmnI GCTCC 2 cut(s) 790, 923
LweI GCATC 3 cut(s) 517, 807, 1009
MaeI CTAG 4 cut(s) 542, 695, 1002, 1120
MaeIII GTNAC 3 cut(s) 373, 580, 1051
MalI GATC 5 cut(s) 209, 281, 386, 715, 866
MboI GATC 5 cut(s) 207, 279, 384, 713, 864
MboII GAAGA 4 cut(s) 92, 444, 485, 579
MflI RGATCY 3 cut(s) 207, 384, 713
MluCI AATT 9 cut(s) 69, 129, 175, 275, 616, 720, 831, 844, 1075
MlyI GAGTC 2 cut(s) 231, 1007
MmeI TCCRAC 3 cut(s) 580, 675, 812
MroI TCCGGA 1 cut(s) 210
MseI TTAA 2 cut(s) 689, 1065
MspCI CTTAAG 1 cut(s) 688
MspI CCGG 6 cut(s) 153, 158, 211, 598, 795, 808
MspR9I CCNGG 4 cut(s) 98, 153, 159, 295
MvaI CCWGG 2 cut(s) 98, 295
MwoI GCNNNNNNNGC 3 cut(s) 498, 804, 1028
NciI CCSGG 2 cut(s) 153, 159
NdeI CATATG 1 cut(s) 781
NdeII GATC 5 cut(s) 207, 279, 384, 713, 864
NlaIII CATG 6 cut(s) 29, 44, 190, 929, 1009, 1028
NlaIV GGNNCC 5 cut(s) 150, 335, 386, 596, 999
NmuCI GTSAC 2 cut(s) 373, 580
NspI RCATGY 2 cut(s) 190, 1009
PfeI GAWTC 2 cut(s) 107, 165
PfoI TCCNGGA 2 cut(s) 96, 157
PleI GAGTC 2 cut(s) 230, 1007
PpsI GAGTC 2 cut(s) 230, 1007
PpuMI RGGWCCY 1 cut(s) 998
Psp5II RGGWCCY 1 cut(s) 998
Psp6I CCWGG 2 cut(s) 96, 293
PspFI CCCAGC 2 cut(s) 439, 451
PspGI CCWGG 2 cut(s) 96, 293
PspN4I GGNNCC 5 cut(s) 150, 335, 386, 596, 999
PspPI GGNCC 4 cut(s) 155, 202, 333, 998
PspPPI RGGWCCY 1 cut(s) 998
PstI CTGCAG 1 cut(s) 665
PsuI RGATCY 3 cut(s) 207, 384, 713
RsaI GTAC 4 cut(s) 19, 311, 972, 1037
RsaNI GTAC 4 cut(s) 18, 310, 971, 1036
Rsr2I CGGWCCG 1 cut(s) 155
RsrII CGGWCCG 1 cut(s) 155
SaqAI TTAA 2 cut(s) 689, 1065
Sau3AI GATC 5 cut(s) 207, 279, 384, 713, 864
Sau96I GGNCC 4 cut(s) 155, 202, 333, 998
SchI GAGTC 2 cut(s) 231, 1007
ScrFI CCNGG 4 cut(s) 98, 153, 159, 295
SfaNI GCATC 3 cut(s) 517, 807, 1009
SfcI CTRYAG 1 cut(s) 661
SinI GGWCC 2 cut(s) 155, 998
SmlI CTYRAG 1 cut(s) 688
SmoI CTYRAG 1 cut(s) 688
Sse9I AATT 9 cut(s) 69, 129, 175, 275, 616, 720, 831, 844, 1075
SspMI CTAG 4 cut(s) 542, 695, 1002, 1120
StyD4I CCNGG 4 cut(s) 96, 151, 157, 293
StyI CCWWGG 2 cut(s) 980, 1091
TaaI ACNGT 4 cut(s) 398, 632, 964, 1046
TaqI TCGA 4 cut(s) 360, 408, 471, 1011
TasI AATT 9 cut(s) 69, 129, 175, 275, 616, 720, 831, 844, 1075
TatI WGTACW 2 cut(s) 17, 1035
TfiI GAWTC 2 cut(s) 107, 165
Tru1I TTAA 2 cut(s) 689, 1065
Tru9I TTAA 2 cut(s) 689, 1065
TscAI CASTG 3 cut(s) 635, 774, 995
TseFI GTSAC 2 cut(s) 373, 580
Tsp45I GTSAC 2 cut(s) 373, 580
TspDTI ATGAA 2 cut(s) 541, 761
TspRI CASTG 3 cut(s) 635, 774, 995
Vha464I CTTAAG 1 cut(s) 688
VpaK11BI GGWCC 2 cut(s) 155, 998
XapI RAATTY 4 cut(s) 129, 616, 844, 1075
XceI RCATGY 2 cut(s) 190, 1009
XspI CTAG 4 cut(s) 542, 695, 1002, 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.