FvH4_3g41750

Protein RFT1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005423
Physical Location & Seq
Reverse (-)
35001968 .. 35007931
5964 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41750.t2

Sequence Viewer

Length: 1362 bp
ATGTCAAATTCTTCGTCATCGTCTGACACCAAATCGTCAAGTGACGCCAGCTCGTCGGATTTCTCACGCACTTTCAAGTACCTATTGGCTACCCAGTTGTGTTCAAGAGGAATTCCATTCATAGTCAATGCTGTGATCCTCAGAAATTTCCTTAAAGTTGAAGACTTTGCGCTTTATGGAGTACAGTTTCCGTTGTTTGTGAATTGCGTTTTGTTTCTCAGCCGGGAGGGGTTTCGGCGAGCATGTCTGCGACAGGACATGAAAAGCGATGGTAGTTCAACAGAAGAAAATATAGCAAAGATCATGAAATTTGCATGGTTGGTTCCGCCGTGTGGAATCATTATTACTACTGCAGCGTCCGGTTTTGTCTTTTGGTGGAAAGGCTTGAGCTTTTCTGATCCATATGGGAAAGGGATCTTGATCACAGGCAGTGCGTGTATATTAGAACTATTGGCAGAGCCCTTGTATATCCTTTCTCAAAACTTGCTTCTGCTTAAACTAAGGCTAGCAGTTGAAACAGCTGCTACCTTTTTTCGCTGCATAACAACAGCCATTCTCTTTGGCATGCGAGTTGACATGGAAAAAGAGATTATATTTGCATTGTCACAAACGGCTTATGGAATGTGCTTGTTCCTAGGGTATTGGGTCTACTTTCTTCGTTTTCGTCCATATAGAAGTTCTGCTCTTTTCCCCTTCAGAGCAAAGGATAAGAAGAACTATGATGGAGAGCTATTAAAGATGTGTGAGTTGTTTGCTTTGCAATCCTGTGTGAAATTGATCCTTCAGGAAGGACAAAATTTTGTGCTTTTGCAGTGGGCAACATATTATAACCAAGCTGTATATGGATTTGTGGACAATTTAGGGAGCCTGGTGGTGAGGATGGTGTTTCTTCCTTTTGAAGAAAGTACCTATGCTACATTTGCAAGGTCTGCATCAGGAAAAGACCCAGATAAAGACAAGAGGTTGGGAAGTAGCCTTACAGAGGCCCTTAAGCTGGTTATGTTAATAGGTCTTATATTTATGGCGTTTGGCCCAAGTTATTCTTATTCTCTCATCAGATTGGTGTATGGTCAAAAATGGAGTGATGGGGAAGCATCAACAGCCCTTGGATACTATTGCTTTTATATTATTGTTTTGGCGATGAATGGAACTTCTGAAGCATTTGTACACGCAGTTGCCAAGAAAAACCAACTCCTATGGTCAAATCTGCCATCTGTGCTGATAACGATCATATATGTAGTACTAAATATCCTGCTGGTAAACTCCGCCGGTGCAGTGGGCTTGATTTTGGCAAATTCATTGAATATGATTTTGAGAATAATATACTCTGGGGCATTCATCAAGCATTACTTCCAGGAATAA

Protein Analysis

454

Amino Acids

50.81

Weight (kDa)

8.92

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rft-1 PF04506 25 - 524 1e-132 Rft protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AccI GTMKAC 1 cut(s) 648
AciI CCGC 2 cut(s) 326, 1266
AclWI GGATC 4 cut(s) 130, 392, 422, 772
AcsI RAATTY 6 cut(s) 7, 111, 145, 308, 796, 1294
AcuI CTGAAG 3 cut(s) 679, 767, 1176
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 5 cut(s) 80, 183, 907, 1167, 1242
AfiI CCNNNNNNNGG 3 cut(s) 332, 982, 994
AflII CTTAAG 1 cut(s) 989
AgsI TTSAA 7 cut(s) 76, 105, 161, 279, 515, 899, 1303
AjnI CCWGG 2 cut(s) 867, 1353
AluBI AGCT 6 cut(s) 51, 390, 521, 730, 836, 994
AluI AGCT 6 cut(s) 51, 390, 521, 730, 836, 994
AlwI GGATC 4 cut(s) 130, 392, 422, 772
AoxI GGCC 2 cut(s) 984, 1030
ApeKI GCWGC 3 cut(s) 353, 521, 537
ApoI RAATTY 6 cut(s) 7, 111, 145, 308, 796, 1294
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspA2I CCTAGG 1 cut(s) 634
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 2 cut(s) 985, 1031
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 886
AsuNHI GCTAGC 1 cut(s) 505
AvrII CCTAGG 1 cut(s) 634
BanII GRGCYC 1 cut(s) 462
BarI GAAGNNNNNNTAC 4 cut(s) 961, 993, 1149, 1181
BbsI GAAGAC 1 cut(s) 168
BbvI GCAGC 3 cut(s) 365, 508, 524
BccI CCATC 5 cut(s) 263, 716, 874, 1079, 1219
BceAI ACGGC 2 cut(s) 313, 627
BciT130I CCWGG 2 cut(s) 869, 1355
BciVI GTATCC 1 cut(s) 1103
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 2 cut(s) 506, 635
BfmI CTRYAG 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 989
BfuI GTATCC 1 cut(s) 1103
BisI GCNGC 3 cut(s) 354, 522, 538
BlnI CCTAGG 1 cut(s) 634
BlsI GCNGC 3 cut(s) 355, 523, 539
BmcAI AGTACT 1 cut(s) 1242
Bme1390I CCNGG 3 cut(s) 224, 869, 1355
BmgT120I GGNCC 2 cut(s) 985, 1031
BmiI GGNNCC 2 cut(s) 324, 866
BmrFI CCNGG 3 cut(s) 224, 869, 1355
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 941, 1103
BmtI GCTAGC 1 cut(s) 509
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 224
BsaBI GATNNNNATC 2 cut(s) 419, 1226
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 634, 1105
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 982, 994
Bse118I RCCGGY 1 cut(s) 1268
Bse1I ACTGG 1 cut(s) 94
Bse8I GATNNNNATC 2 cut(s) 419, 1226
BseBI CCWGG 2 cut(s) 869, 1355
BseDI CCNNGG 2 cut(s) 634, 1105
BseGI GGATG 1 cut(s) 885
BseJI GATNNNNATC 2 cut(s) 419, 1226
BseLI CCNNNNNNNGG 3 cut(s) 332, 982, 994
BseMII CTCAG 2 cut(s) 154, 232
BseNI ACTGG 1 cut(s) 94
BseXI GCAGC 3 cut(s) 365, 508, 524
BsgI GTGCAG 1 cut(s) 1293
BshFI GGCC 2 cut(s) 986, 1032
BsiSI CCGG 3 cut(s) 223, 360, 1269
BslI CCNNNNNNNGG 3 cut(s) 332, 982, 994
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 2 cut(s) 986, 1032
Bsp1286I GDGCHC 1 cut(s) 462
Bsp1407I TGTACA 1 cut(s) 1165
Bsp143I GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
BspACI CCGC 2 cut(s) 326, 1266
BspANI GGCC 2 cut(s) 986, 1032
BspCNI CTCAG 2 cut(s) 153, 231
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 2 cut(s) 324, 866
BspMAI CTGCAG 1 cut(s) 355
BspOI GCTAGC 1 cut(s) 509
BspPI GGATC 4 cut(s) 130, 392, 422, 772
BspTI CTTAAG 1 cut(s) 989
BsrFI RCCGGY 1 cut(s) 1268
BsrGI TGTACA 1 cut(s) 1165
BsrI ACTGG 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 1268
BssECI CCNNGG 2 cut(s) 634, 1105
BssMI GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 2 cut(s) 634, 1105
Bst2UI CCWGG 2 cut(s) 869, 1355
Bst4CI ACNGT 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 989
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 1 cut(s) 1165
BstC8I GCNNGC 4 cut(s) 49, 240, 507, 566
BstDEI CTNAG 3 cut(s) 140, 218, 500
BstENI CCTNNNNNAGG 1 cut(s) 980
BstF5I GGATG 1 cut(s) 885
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 7 cut(s) 138, 303, 400, 417, 423, 780, 1230
BstMBI GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
BstMWI GCNNNNNNNGC 4 cut(s) 920, 929, 1100, 1216
BstNI CCWGG 2 cut(s) 869, 1355
BstNSI RCATGY 2 cut(s) 246, 568
BstSCI CCNGG 3 cut(s) 222, 867, 1353
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 3 cut(s) 365, 508, 524
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuI GTATCC 1 cut(s) 1103
BsuRI GGCC 2 cut(s) 986, 1032
BtgZI GCGATG 2 cut(s) 282, 1154
BtsCI GGATG 1 cut(s) 885
BtsI GCAGTG 3 cut(s) 436, 818, 1281
BtsIMutI CAGTG 3 cut(s) 436, 818, 1281
Cac8I GCNNGC 4 cut(s) 49, 240, 507, 566
CciI TCATGA 1 cut(s) 303
CfoI GCGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 1268
Cfr13I GGNCC 2 cut(s) 985, 1031
CseI GACGC 2 cut(s) 53, 345
Csp6I GTAC 5 cut(s) 79, 182, 906, 1166, 1241
CviAII CATG 6 cut(s) 243, 259, 304, 315, 565, 577
CviQI GTAC 5 cut(s) 79, 182, 906, 1166, 1241
DdeI CTNAG 3 cut(s) 140, 218, 500
DpnI GATC 7 cut(s) 137, 302, 399, 416, 422, 779, 1229
DpnII GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
EciI GGCGGA 2 cut(s) 315, 1255
Eco130I CCWWGG 2 cut(s) 634, 1105
Eco24I GRGCYC 1 cut(s) 462
Eco57I CTGAAG 3 cut(s) 679, 767, 1176
EcoNI CCTNNNNNAGG 1 cut(s) 980
EcoO109I RGGNCCY 1 cut(s) 985
EcoRI GAATTC 1 cut(s) 111
EcoRII CCWGG 2 cut(s) 867, 1353
EcoT14I CCWWGG 2 cut(s) 634, 1105
EcoT38I GRGCYC 1 cut(s) 462
ErhI CCWWGG 2 cut(s) 634, 1105
FaeI CATG 6 cut(s) 246, 262, 307, 318, 568, 580
FalI AAGNNNNNCTT 3 cut(s) 1027, 1059, 1334
FatI CATG 6 cut(s) 242, 258, 303, 314, 564, 576
FauNDI CATATG 1 cut(s) 403
FbaI TGATCA 1 cut(s) 420
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 3 cut(s) 354, 522, 538
FokI GGATG 1 cut(s) 892
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 3 cut(s) 354, 522, 538
FspBI CTAG 2 cut(s) 506, 635
GlaI GCGC 1 cut(s) 171
GluI GCNGC 3 cut(s) 354, 522, 538
HaeIII GGCC 2 cut(s) 986, 1032
HapII CCGG 3 cut(s) 223, 360, 1269
HgaI GACGC 2 cut(s) 53, 345
HhaI GCGC 1 cut(s) 172
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 6 cut(s) 246, 262, 307, 318, 568, 580
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HinfI GANTC 1 cut(s) 336
HpaII CCGG 3 cut(s) 223, 360, 1269
HphI GGTGA 1 cut(s) 886
Hpy166II GTNNAC 5 cut(s) 574, 649, 853, 1168, 1261
Hpy188I TCNGA 7 cut(s) 25, 58, 143, 397, 698, 1058, 1156
Hpy188III TCNNGA 5 cut(s) 105, 304, 418, 785, 936
Hpy8I GTNNAC 5 cut(s) 574, 649, 853, 1168, 1261
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 3 cut(s) 703, 782, 791
HpyCH4III ACNGT 1 cut(s) 186
HpyCH4V TGCA 9 cut(s) 314, 353, 540, 599, 760, 811, 923, 932, 1274
HpyF10VI GCNNNNNNNGC 4 cut(s) 920, 929, 1100, 1216
HpyF3I CTNAG 3 cut(s) 140, 218, 500
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 6 cut(s) 246, 262, 307, 318, 568, 580
HspAI GCGC 1 cut(s) 170
Ksp22I TGATCA 1 cut(s) 420
Kzo9I GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
LmnI GCTCC 1 cut(s) 864
Lsp1109I GCAGC 3 cut(s) 365, 508, 524
LweI GCATC 2 cut(s) 941, 1103
MaeI CTAG 2 cut(s) 506, 635
MaeIII GTNAC 2 cut(s) 41, 603
MalI GATC 7 cut(s) 137, 302, 399, 416, 422, 779, 1229
MboI GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
MboII GAAGA 7 cut(s) 3, 173, 296, 647, 724, 881, 911
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 9 cut(s) 7, 111, 145, 202, 308, 773, 796, 856, 1294
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 6 cut(s) 101, 149, 220, 870, 954, 976
MseI TTAA 5 cut(s) 153, 495, 734, 990, 1004
MspA1I CMGCKG 1 cut(s) 521
MspCI CTTAAG 1 cut(s) 989
MspI CCGG 3 cut(s) 223, 360, 1269
MspR9I CCNGG 3 cut(s) 224, 869, 1355
Mva1269I GAATGC 1 cut(s) 1334
MvaI CCWGG 2 cut(s) 869, 1355
MwoI GCNNNNNNNGC 4 cut(s) 920, 929, 1100, 1216
NciI CCSGG 1 cut(s) 224
NdeI CATATG 1 cut(s) 403
NdeII GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
NheI GCTAGC 1 cut(s) 505
NlaIII CATG 6 cut(s) 246, 262, 307, 318, 568, 580
NlaIV GGNNCC 2 cut(s) 324, 866
NmuCI GTSAC 2 cut(s) 41, 603
NspI RCATGY 2 cut(s) 246, 568
PaeI GCATGC 1 cut(s) 568
PagI TCATGA 1 cut(s) 303
PctI GAATGC 1 cut(s) 1334
PfeI GAWTC 1 cut(s) 336
PfoI TCCNGGA 1 cut(s) 1353
PkrI GCNGC 3 cut(s) 355, 523, 539
PsiI TTATAA 1 cut(s) 828
Psp6I CCWGG 2 cut(s) 867, 1353
PspGI CCWGG 2 cut(s) 867, 1353
PspN4I GGNNCC 2 cut(s) 324, 866
PspPI GGNCC 2 cut(s) 985, 1031
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 414
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 5 cut(s) 80, 183, 907, 1167, 1242
RsaNI GTAC 5 cut(s) 79, 182, 906, 1166, 1241
SaqAI TTAA 5 cut(s) 153, 495, 734, 990, 1004
SatI GCNGC 3 cut(s) 354, 522, 538
Sau3AI GATC 7 cut(s) 135, 300, 397, 414, 420, 777, 1227
Sau96I GGNCC 2 cut(s) 985, 1031
ScaI AGTACT 1 cut(s) 1242
ScrFI CCNGG 3 cut(s) 224, 869, 1355
SduI GDGCHC 1 cut(s) 462
SfaNI GCATC 2 cut(s) 941, 1103
SfcI CTRYAG 1 cut(s) 351
SgrAI CRCCGGYG 1 cut(s) 1268
SmlI CTYRAG 2 cut(s) 385, 989
SmoI CTYRAG 2 cut(s) 385, 989
SphI GCATGC 1 cut(s) 568
Sse9I AATT 9 cut(s) 7, 111, 145, 202, 308, 773, 796, 856, 1294
SsiI CCGC 2 cut(s) 326, 1266
SspMI CTAG 2 cut(s) 506, 635
StyD4I CCNGG 3 cut(s) 222, 867, 1353
StyI CCWWGG 2 cut(s) 634, 1105
TaaI ACNGT 1 cut(s) 186
TasI AATT 9 cut(s) 7, 111, 145, 202, 308, 773, 796, 856, 1294
TatI WGTACW 3 cut(s) 181, 1165, 1240
TfiI GAWTC 1 cut(s) 336
Tru1I TTAA 5 cut(s) 153, 495, 734, 990, 1004
Tru9I TTAA 5 cut(s) 153, 495, 734, 990, 1004
TscAI CASTG 3 cut(s) 436, 818, 1281
TseFI GTSAC 2 cut(s) 41, 603
TseI GCWGC 3 cut(s) 353, 521, 537
Tsp45I GTSAC 2 cut(s) 41, 603
TspDTI ATGAA 6 cut(s) 109, 275, 320, 1157, 1287, 1327
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 3 cut(s) 436, 818, 1281
Vha464I CTTAAG 1 cut(s) 989
XagI CCTNNNNNAGG 1 cut(s) 980
XapI RAATTY 6 cut(s) 7, 111, 145, 308, 796, 1294
XceI RCATGY 2 cut(s) 246, 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XmaJI CCTAGG 1 cut(s) 634
XmiI GTMKAC 1 cut(s) 648
XspI CTAG 2 cut(s) 506, 635
ZrmI AGTACT 1 cut(s) 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.