Rw5G045590

Protein RFT1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005423
Physical Location & Seq
Reverse (-)
79593031 .. 79600838
7808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045590.1

Sequence Viewer

Length: 1332 bp
ATGAAATTTGCGTGGTTGGTTCCGCCGTGTGGAATTATTATTACTACTGCAGCGTCTGGTTTTGTCTTTTGGTGGAAAGGCTTGAGCTTTTCTGATCCATATGGGCAAGGGATCTTGATTACTGGCGGTGCGTGTATATTAGAACTATTGGCAGAGCCCTTGTATATCCTATCTCAAAACTTGTTGCTGCTTAAATTAAGGCTGGCAGTTGAAACTGCCGCTACTTTTTTTCGCTGCATAACAACAGCCATTCTCTTTGGCATGCGAGCTGACATGGAAAAAGAGATTATATTTGCATTGTCACAGACTGCTTATGGACTGTGCTTGTTCTCAGGGTATTGGGTCTACTTTCTTCGTTTTCGTCCATATAGAAGTTCTGCTCTTTTCCCCTTCAGAGCAAAGAATAAGAAGAATTATGATGGGGAGCTGTTAAAGATGTGTGAGTTGTTTGCTTTGCAATCCTGTGTGAAATTGATCCTTCAGGAAGGACAAAATTTTGTTCTTTTACAGTGGGCAACATATTATAACCAAGCTGTATATGGATTTGTGGACAATTTAGGGAGCCTGGTGGTGAGGATGGTGTTTCTTCCTTTTGAAGAAAGTACCTATGCTACATTTGCAAGGTCTGCATCAGGAAAAGACCCAGATACGGACAAGAGGTTGGGAGGTAGCCTTACAGAAGCCCTTAAGCTGGTTTTGTTAATAGGTCTTATATTTATGGCGTTTGGCCCGAGTTATGCTTATTCTCTCATCAGATTGGTGTATGGTCAAAAATGGAGTGATGGGGAAGCATCAACAGCCCTGGGATATTATTGCTTTTATATTATTGTTTTGGCTGTGAATGGAACTTCTGAAGCATTTGTACATGCAGTTGCCAAGAAGAACCAACTTCTGTGGTCAAATCTTCCATCTGTTCTGATAACAATTATATATGTGGTACTGAATATCCTGCTGGTAAACTCCGCTGGCGCAGTTGGCTTAATTTTGGCAAATTCATTAAATATGATTTTGAGAATATTATACTCTGGGGCATTCATCAAGCATTACTTCCAGGATTCTTCCTCATTTTCTTTTTACAAATGCTTACCTGAAGGATGGAAGGTCTTGCTGTTTTCAGGAATAACTACTATCATTTCTGAGAGAATATTTCTAGACAGGAAGAATTTCTGGGCGACTTTTGTTGTTCATTTCACCATTGGGGTCGCTTGCTTCTGCACTTCGGCATTTGTAGTATATCGTCATGAGAGATCCCTTATCAACAAAATCATTCCCATCAACAGGATTTTCCCAAGCATCAACAAAATCGTCCGCTTCCGTGATCACTCAGACTGA

Protein Analysis

443

Amino Acids

49.97

Weight (kDa)

9.14

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rft-1 PF04506 2 - 423 6.7e-106 Rft protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AccI GTMKAC 1 cut(s) 345
AciI CCGC 5 cut(s) 23, 126, 219, 963, 1309
AclWI GGATC 4 cut(s) 89, 119, 469, 1242
AcsI RAATTY 4 cut(s) 5, 493, 991, 1162
AcuI CTGAAG 4 cut(s) 376, 464, 873, 1110
AfaI GTAC 3 cut(s) 604, 864, 939
AfiI CCNNNNNNNGG 4 cut(s) 29, 649, 691, 1278
AflII CTTAAG 1 cut(s) 686
AgsI TTSAA 2 cut(s) 212, 596
AjnI CCWGG 3 cut(s) 564, 801, 1050
AluBI AGCT 5 cut(s) 87, 269, 427, 533, 691
AluI AGCT 5 cut(s) 87, 269, 427, 533, 691
AlwI GGATC 4 cut(s) 89, 119, 469, 1242
AlwNI CAGNNNCTG 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 1 cut(s) 727
ApeKI GCWGC 3 cut(s) 50, 187, 234
ApoI RAATTY 4 cut(s) 5, 493, 991, 1162
Asp700I GAANNNNTTC 1 cut(s) 1163
AspLEI GCGC 1 cut(s) 971
AspS9I GGNCC 1 cut(s) 728
AsuHPI GGTGA 2 cut(s) 583, 1183
AvaI CYCGRG 1 cut(s) 730
BanII GRGCYC 1 cut(s) 159
BarI GAAGNNNNNNTAC 2 cut(s) 846, 878
BbvI GCAGC 3 cut(s) 62, 174, 221
BccI CCATC 6 cut(s) 413, 571, 776, 916, 1089, 1280
BceAI ACGGC 1 cut(s) 10
BciT130I CCWGG 3 cut(s) 566, 803, 1052
BclI TGATCA 1 cut(s) 1318
BfaI CTAG 1 cut(s) 1151
BfmI CTRYAG 1 cut(s) 48
BfrI CTTAAG 1 cut(s) 686
BisI GCNGC 4 cut(s) 51, 188, 219, 235
BlsI GCNGC 4 cut(s) 52, 189, 220, 236
Bme1390I CCNGG 3 cut(s) 566, 803, 1052
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 2 cut(s) 21, 563
BmrFI CCNGG 3 cut(s) 566, 803, 1052
BmsI GCATC 3 cut(s) 638, 800, 1302
BpuEI CTTGAG 1 cut(s) 103
BsaJI CCNNGG 2 cut(s) 801, 802
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 649, 691, 1278
Bse1I ACTGG 1 cut(s) 127
BseBI CCWGG 3 cut(s) 566, 803, 1052
BseDI CCNNGG 2 cut(s) 801, 802
BseGI GGATG 2 cut(s) 582, 1100
BseLI CCNNNNNNNGG 4 cut(s) 29, 649, 691, 1278
BseMII CTCAG 2 cut(s) 345, 1128
BseNI ACTGG 1 cut(s) 127
BseXI GCAGC 3 cut(s) 62, 174, 221
BsgI GTGCAG 1 cut(s) 1198
BshFI GGCC 1 cut(s) 729
BsiHKCI CYCGRG 1 cut(s) 730
BslI CCNNNNNNNGG 4 cut(s) 29, 649, 691, 1278
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 1 cut(s) 729
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 1 cut(s) 159
Bsp1407I TGTACA 1 cut(s) 862
Bsp143I GATC 5 cut(s) 94, 111, 474, 1247, 1318
BspACI CCGC 5 cut(s) 23, 126, 219, 963, 1309
BspANI GGCC 1 cut(s) 729
BspCNI CTCAG 2 cut(s) 344, 1129
BspHI TCATGA 1 cut(s) 1240
BspLI GGNNCC 2 cut(s) 21, 563
BspMAI CTGCAG 1 cut(s) 52
BspPI GGATC 4 cut(s) 89, 119, 469, 1242
BspTI CTTAAG 1 cut(s) 686
BsrGI TGTACA 1 cut(s) 862
BsrI ACTGG 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 801, 802
BssMI GATC 5 cut(s) 94, 111, 474, 1247, 1318
Bst2UI CCWGG 3 cut(s) 566, 803, 1052
Bst4CI ACNGT 2 cut(s) 321, 510
BstAFI CTTAAG 1 cut(s) 686
BstAPI GCANNNNNTGC 1 cut(s) 626
BstAUI TGTACA 1 cut(s) 862
BstC8I GCNNGC 5 cut(s) 204, 263, 267, 967, 1207
BstDEI CTNAG 3 cut(s) 331, 1137, 1324
BstF5I GGATG 2 cut(s) 582, 1100
BstHHI GCGC 1 cut(s) 971
BstKTI GATC 5 cut(s) 97, 114, 477, 1250, 1321
BstMBI GATC 5 cut(s) 94, 111, 474, 1247, 1318
BstMWI GCNNNNNNNGC 4 cut(s) 617, 626, 797, 975
BstNI CCWGG 3 cut(s) 566, 803, 1052
BstNSI RCATGY 2 cut(s) 265, 869
BstSCI CCNGG 3 cut(s) 564, 801, 1050
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 3 cut(s) 62, 174, 221
BstX2I RGATCY 2 cut(s) 111, 1247
BstYI RGATCY 2 cut(s) 111, 1247
BsuRI GGCC 1 cut(s) 729
BtsCI GGATG 2 cut(s) 582, 1100
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 5 cut(s) 204, 263, 267, 967, 1207
CaiI CAGNNNCTG 1 cut(s) 56
CciI TCATGA 1 cut(s) 1240
CfoI GCGC 1 cut(s) 971
Cfr13I GGNCC 1 cut(s) 728
CseI GACGC 1 cut(s) 42
Csp6I GTAC 3 cut(s) 603, 863, 938
CspCI CAANNNNNGTGG 2 cut(s) 875, 910
CviAII CATG 4 cut(s) 262, 274, 866, 1241
CviQI GTAC 3 cut(s) 603, 863, 938
DdeI CTNAG 3 cut(s) 331, 1137, 1324
DpnI GATC 5 cut(s) 96, 113, 476, 1249, 1320
DpnII GATC 5 cut(s) 94, 111, 474, 1247, 1318
EciI GGCGGA 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 159
Eco57I CTGAAG 4 cut(s) 376, 464, 873, 1110
Eco88I CYCGRG 1 cut(s) 730
EcoRII CCWGG 3 cut(s) 564, 801, 1050
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 4 cut(s) 265, 277, 869, 1244
FalI AAGNNNNNCTT 2 cut(s) 1031, 1063
FatI CATG 4 cut(s) 261, 273, 865, 1240
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 1 cut(s) 1318
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 51, 188, 219, 235
FokI GGATG 2 cut(s) 589, 1107
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 51, 188, 219, 235
FspBI CTAG 1 cut(s) 1151
GlaI GCGC 1 cut(s) 970
GluI GCNGC 4 cut(s) 51, 188, 219, 235
HaeIII GGCC 1 cut(s) 729
HgaI GACGC 1 cut(s) 42
HhaI GCGC 1 cut(s) 971
Hin1II CATG 4 cut(s) 265, 277, 869, 1244
Hin6I GCGC 1 cut(s) 969
HinP1I GCGC 1 cut(s) 969
HinfI GANTC 1 cut(s) 1055
HphI GGTGA 2 cut(s) 583, 1183
Hpy166II GTNNAC 3 cut(s) 346, 550, 958
Hpy188I TCNGA 7 cut(s) 94, 395, 755, 853, 918, 1138, 1327
Hpy188III TCNNGA 6 cut(s) 115, 482, 633, 1116, 1151, 1241
Hpy8I GTNNAC 3 cut(s) 346, 550, 958
HpyAV CCTTC 5 cut(s) 400, 479, 488, 1085, 1093
HpyCH4III ACNGT 2 cut(s) 321, 510
HpyCH4V TGCA 8 cut(s) 50, 237, 296, 457, 620, 629, 869, 1215
HpyF10VI GCNNNNNNNGC 4 cut(s) 617, 626, 797, 975
HpyF3I CTNAG 3 cut(s) 331, 1137, 1324
Hsp92II CATG 4 cut(s) 265, 277, 869, 1244
HspAI GCGC 1 cut(s) 969
Ksp22I TGATCA 1 cut(s) 1318
Kzo9I GATC 5 cut(s) 94, 111, 474, 1247, 1318
LmnI GCTCC 2 cut(s) 424, 561
Lsp1109I GCAGC 3 cut(s) 62, 174, 221
LweI GCATC 3 cut(s) 638, 800, 1302
MaeI CTAG 1 cut(s) 1151
MaeIII GTNAC 1 cut(s) 300
MalI GATC 5 cut(s) 96, 113, 476, 1249, 1320
MboI GATC 5 cut(s) 94, 111, 474, 1247, 1318
MboII GAAGA 8 cut(s) 344, 421, 578, 608, 892, 896, 1050, 1171
MflI RGATCY 2 cut(s) 111, 1247
MhlI GDGCHC 1 cut(s) 159
MnlI CCTC 4 cut(s) 567, 651, 659, 1072
MroXI GAANNNNTTC 1 cut(s) 1163
MseI TTAA 7 cut(s) 192, 197, 431, 687, 701, 980, 998
MspA1I CMGCKG 1 cut(s) 965
MspCI CTTAAG 1 cut(s) 686
MspR9I CCNGG 3 cut(s) 566, 803, 1052
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 3 cut(s) 566, 803, 1052
MwoI GCNNNNNNNGC 4 cut(s) 617, 626, 797, 975
NdeI CATATG 1 cut(s) 100
NdeII GATC 5 cut(s) 94, 111, 474, 1247, 1318
NlaIII CATG 4 cut(s) 265, 277, 869, 1244
NlaIV GGNNCC 2 cut(s) 21, 563
NmuCI GTSAC 1 cut(s) 300
NspI RCATGY 2 cut(s) 265, 869
PaeI GCATGC 1 cut(s) 265
PagI TCATGA 1 cut(s) 1240
PasI CCCWGGG 1 cut(s) 802
PctI GAATGC 1 cut(s) 1031
PdmI GAANNNNTTC 1 cut(s) 1163
PfeI GAWTC 1 cut(s) 1055
PfoI TCCNGGA 1 cut(s) 1050
PkrI GCNGC 4 cut(s) 52, 189, 220, 236
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 3 cut(s) 564, 801, 1050
PspGI CCWGG 3 cut(s) 564, 801, 1050
PspN4I GGNNCC 2 cut(s) 21, 563
PspPI GGNCC 1 cut(s) 728
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 2 cut(s) 111, 1247
RsaI GTAC 3 cut(s) 604, 864, 939
RsaNI GTAC 3 cut(s) 603, 863, 938
SaqAI TTAA 7 cut(s) 192, 197, 431, 687, 701, 980, 998
SatI GCNGC 4 cut(s) 51, 188, 219, 235
Sau3AI GATC 5 cut(s) 94, 111, 474, 1247, 1318
Sau96I GGNCC 1 cut(s) 728
ScrFI CCNGG 3 cut(s) 566, 803, 1052
SduI GDGCHC 1 cut(s) 159
SfaNI GCATC 3 cut(s) 638, 800, 1302
SfcI CTRYAG 1 cut(s) 48
SmlI CTYRAG 2 cut(s) 82, 686
SmoI CTYRAG 2 cut(s) 82, 686
SphI GCATGC 1 cut(s) 265
SsiI CCGC 5 cut(s) 23, 126, 219, 963, 1309
SspI AATATT 2 cut(s) 1017, 1146
SspMI CTAG 1 cut(s) 1151
StyD4I CCNGG 3 cut(s) 564, 801, 1050
TaaI ACNGT 2 cut(s) 321, 510
TatI WGTACW 1 cut(s) 862
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 1055
Tru1I TTAA 7 cut(s) 192, 197, 431, 687, 701, 980, 998
Tru9I TTAA 7 cut(s) 192, 197, 431, 687, 701, 980, 998
TscAI CASTG 1 cut(s) 515
TseFI GTSAC 1 cut(s) 300
TseI GCWGC 3 cut(s) 50, 187, 234
Tsp45I GTSAC 1 cut(s) 300
TspDTI ATGAA 4 cut(s) 17, 984, 1024, 1175
TspGWI ACGGA 2 cut(s) 665, 1304
TspRI CASTG 1 cut(s) 515
Vha464I CTTAAG 1 cut(s) 686
XapI RAATTY 4 cut(s) 5, 493, 991, 1162
XbaI TCTAGA 1 cut(s) 1150
XceI RCATGY 2 cut(s) 265, 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 1163
XspI CTAG 1 cut(s) 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.