Rh5DG525600

Protein RFT1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005423
Physical Location & Seq
Reverse (-)
81335323 .. 81341361
6039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG525600.1

Sequence Viewer

Length: 1635 bp
ATGTCCAATTCTCCGTCGTCGTCTGAATCCAAATCCCCAAGTGACGCCAGCTCGTCGGATTTCTCTCGCACTTTCAAGTACCTATTGGCTACCCAGTTGTGTTCAAGAGGAATTCCATTCATAGTCAATGCTGTGATCCTCAGAAATTTCCTTAAAGTTGAAGACTTTGCGCTCTATGGAGTACAGTTTCCGCTATTTGTGAATTGCGTGTTGTTTCTCAGCCGGGAGGGGTTTCGGCGAGCATGTCTGCGACAGGACATGAAAAGCGATGGTACTTCAACAGAAGAAAACATAGCAAAGATCATGAAATTTGCGTGGTTGGTTCCACCGTGTGGAATTATTATTACTACTGCAGCGTCTGGTTTTGTCTTTTGGTGGAAAGGCTTGAGCTTTTCTGATCCATATGGACAAGGGATCTTGATTACTGGCGGTGCATGTATATTAGAACTATTGGCAGAGCCCTTGTATATCCTATCTCAAAACTTGCTGCTGCTTAAATTAAGGCTGGCAGTTGAAACTGCCGCTACTTTTTTTCGCTGCATAACAACAGCCGTTCTCTTTGGCATGCGAGCTGACATGGAAAAAGAGATTATATTTGCATTGTCACAGACTGCTTATGGACTGTGCTTGTTCTCAGGGTATTGGGTCTACTTTCTTCGTTTTCGTCCATATAGAAGTTCTGCTCTTTTCCCCTTCAGAGCAAAGAATAAGAAGAATTATGATGGGGAGCTGTTAAAGATGTGTGAGTTGTTTGCTTTGCAATCCTGTGTGAAACTGATCCTTCAGGAAGGACAAAATTTTGTTCTTTTACAGTGGGCAACATATTATAACCAAGCTGTATATGGATTTGTGGACAATTTAGGGAGCCTGGTGGTGAGGATGGTGTTTCTTCCTTTTGAAGAAAGTACCTATGCTACATTTGCAAGGTCTGCATCAGGAAAAGACCCAGATAAGGACAAGAGGTTGGGAAGTAGCCTTACAGAGGCCCTTAAGCTGGTTTTGTTAATAGGTCTTATATTTATGGCGTTTGGCCCGAGTTATGCTTATTCTCTCATCAGATTGGTGTATGGTCAAAAATGGAGTGATGGGGAAGCATCAACAGCCCTGGGATATTATTGCTTTTATATTATTGTTTTGGCTATGAATGGAACTTCTGAAGCATTTGTACATGCAGTTGCCAAGAAGAACCAGCTTCTGTGGTCAAATCTTCCATCTGTTCTGATAACAATTATATATGTAGTACTGAATATCCTGCTGGTAAACTCCGCTGGCGCAGTTGGCTTAATTTTGGCAAATTCATTAAATATGATTTTGAGAATATTATACTCTGGGGCATTCATCAAGCATTACTTCCAGGATTCTTCCTCATTTTCTTTTTACAAATGCTTACCTGAAGGATGGAAGGTCTTGCTGTTTTCAGGAATAACTACTATCATTTCTGAGAGAATATTTCTAGACAGGAAGAATTTCTGGGCGACTTTTGTTGTTCATTTTACCATTGGGGTCGCTTGCTTCTGCACTTCGGCATTTGTCATATATCGTCATGAGAGATCCCTTATCAACAAAATCATTCCCATCAACAGGATTTTTCCAAGCATCAACAAAATTGTCCGCTTCCGTGATCACTCAGACTGA

Protein Analysis

544

Amino Acids

61.33

Weight (kDa)

9.15

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rft-1 PF04506 25 - 524 8.4e-132 Rft protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AccB7I CCANNNNNTGG 1 cut(s) 332
AccI GTMKAC 1 cut(s) 648
AciI CCGC 5 cut(s) 191, 429, 522, 1266, 1612
AclWI GGATC 5 cut(s) 130, 392, 422, 772, 1545
AcsI RAATTY 6 cut(s) 111, 145, 308, 796, 1294, 1465
AcuI CTGAAG 4 cut(s) 679, 767, 1176, 1413
AcyI GRCGYC 1 cut(s) 45
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 6 cut(s) 80, 183, 274, 907, 1167, 1242
AfiI CCNNNNNNNGG 5 cut(s) 332, 952, 982, 994, 1581
AflII CTTAAG 1 cut(s) 989
AgsI TTSAA 6 cut(s) 76, 105, 161, 279, 515, 899
AjnI CCWGG 3 cut(s) 867, 1104, 1353
AluBI AGCT 7 cut(s) 51, 390, 572, 730, 836, 994, 1192
AluI AGCT 7 cut(s) 51, 390, 572, 730, 836, 994, 1192
AlwI GGATC 5 cut(s) 130, 392, 422, 772, 1545
AlwNI CAGNNNCTG 2 cut(s) 359, 1195
Ama87I CYCGRG 1 cut(s) 1033
AoxI GGCC 2 cut(s) 984, 1030
ApeKI GCWGC 4 cut(s) 353, 487, 490, 537
ApoI RAATTY 6 cut(s) 111, 145, 308, 796, 1294, 1465
Asp700I GAANNNNTTC 1 cut(s) 1466
AspLEI GCGC 2 cut(s) 172, 1274
AspS9I GGNCC 2 cut(s) 985, 1031
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 886
AvaI CYCGRG 1 cut(s) 1033
BanII GRGCYC 1 cut(s) 462
BarI GAAGNNNNNNTAC 4 cut(s) 961, 993, 1149, 1181
BbsI GAAGAC 1 cut(s) 168
BbvI GCAGC 4 cut(s) 365, 474, 477, 524
BccI CCATC 7 cut(s) 263, 716, 874, 1079, 1219, 1392, 1583
BceAI ACGGC 1 cut(s) 536
BciT130I CCWGG 3 cut(s) 869, 1106, 1355
BclI TGATCA 1 cut(s) 1621
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 1 cut(s) 1454
BfmI CTRYAG 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 989
BisI GCNGC 5 cut(s) 354, 488, 491, 522, 538
BlsI GCNGC 5 cut(s) 355, 489, 492, 523, 539
BmcAI AGTACT 1 cut(s) 1242
Bme1390I CCNGG 4 cut(s) 224, 869, 1106, 1355
BmeT110I CYCGRG 1 cut(s) 1033
BmgT120I GGNCC 2 cut(s) 985, 1031
BmiI GGNNCC 2 cut(s) 324, 866
BmrFI CCNGG 4 cut(s) 224, 869, 1106, 1355
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 3 cut(s) 941, 1103, 1605
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 1104, 1105
Bsc4I CCNNNNNNNGG 5 cut(s) 332, 952, 982, 994, 1581
Bse1I ACTGG 2 cut(s) 94, 430
BseBI CCWGG 3 cut(s) 869, 1106, 1355
BseDI CCNNGG 2 cut(s) 1104, 1105
BseGI GGATG 2 cut(s) 885, 1403
BseLI CCNNNNNNNGG 5 cut(s) 332, 952, 982, 994, 1581
BseMII CTCAG 4 cut(s) 154, 232, 648, 1431
BseNI ACTGG 2 cut(s) 94, 430
BseXI GCAGC 4 cut(s) 365, 474, 477, 524
BsgI GTGCAG 1 cut(s) 1501
BshFI GGCC 2 cut(s) 986, 1032
BsiHKCI CYCGRG 1 cut(s) 1033
BsiSI CCGG 1 cut(s) 223
BslI CCNNNNNNNGG 5 cut(s) 332, 952, 982, 994, 1581
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 2 cut(s) 986, 1032
BsoBI CYCGRG 1 cut(s) 1033
Bsp1286I GDGCHC 1 cut(s) 462
Bsp1407I TGTACA 1 cut(s) 1165
Bsp143I GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
BspACI CCGC 5 cut(s) 191, 429, 522, 1266, 1612
BspANI GGCC 2 cut(s) 986, 1032
BspCNI CTCAG 4 cut(s) 153, 231, 647, 1432
BspHI TCATGA 2 cut(s) 303, 1543
BspLI GGNNCC 2 cut(s) 324, 866
BspMAI CTGCAG 1 cut(s) 355
BspPI GGATC 5 cut(s) 130, 392, 422, 772, 1545
BspTI CTTAAG 1 cut(s) 989
BsrGI TGTACA 1 cut(s) 1165
BsrI ACTGG 2 cut(s) 94, 430
BssECI CCNNGG 2 cut(s) 1104, 1105
BssMI GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 3 cut(s) 869, 1106, 1355
Bst4CI ACNGT 4 cut(s) 186, 330, 624, 813
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 989
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 1 cut(s) 1165
BstC8I GCNNGC 7 cut(s) 49, 240, 507, 566, 570, 1270, 1510
BstDEI CTNAG 5 cut(s) 140, 218, 634, 1440, 1627
BstENI CCTNNNNNAGG 1 cut(s) 980
BstF5I GGATG 2 cut(s) 885, 1403
BstHHI GCGC 2 cut(s) 172, 1274
BstKTI GATC 7 cut(s) 138, 303, 400, 417, 780, 1553, 1624
BstMBI GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
BstMWI GCNNNNNNNGC 4 cut(s) 920, 929, 1100, 1278
BstNI CCWGG 3 cut(s) 869, 1106, 1355
BstNSI RCATGY 4 cut(s) 246, 438, 568, 1172
BstSCI CCNGG 4 cut(s) 222, 867, 1104, 1353
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 4 cut(s) 365, 474, 477, 524
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 2 cut(s) 414, 1550
BstYI RGATCY 2 cut(s) 414, 1550
BsuRI GGCC 2 cut(s) 986, 1032
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 2 cut(s) 885, 1403
BtsIMutI CAGTG 1 cut(s) 818
Cac8I GCNNGC 7 cut(s) 49, 240, 507, 566, 570, 1270, 1510
CaiI CAGNNNCTG 2 cut(s) 359, 1195
CciI TCATGA 2 cut(s) 303, 1543
CfoI GCGC 2 cut(s) 172, 1274
Cfr13I GGNCC 2 cut(s) 985, 1031
CseI GACGC 2 cut(s) 53, 345
Csp6I GTAC 6 cut(s) 79, 182, 273, 906, 1166, 1241
CviAII CATG 8 cut(s) 243, 259, 304, 435, 565, 577, 1169, 1544
CviQI GTAC 6 cut(s) 79, 182, 273, 906, 1166, 1241
DdeI CTNAG 5 cut(s) 140, 218, 634, 1440, 1627
DpnI GATC 7 cut(s) 137, 302, 399, 416, 779, 1552, 1623
DpnII GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
DraIII CACNNNGTG 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 462
Eco57I CTGAAG 4 cut(s) 679, 767, 1176, 1413
Eco88I CYCGRG 1 cut(s) 1033
EcoNI CCTNNNNNAGG 1 cut(s) 980
EcoO109I RGGNCCY 1 cut(s) 985
EcoRI GAATTC 1 cut(s) 111
EcoRII CCWGG 3 cut(s) 867, 1104, 1353
EcoT38I GRGCYC 1 cut(s) 462
FaeI CATG 8 cut(s) 246, 262, 307, 438, 568, 580, 1172, 1547
FalI AAGNNNNNCTT 2 cut(s) 1334, 1366
FatI CATG 8 cut(s) 242, 258, 303, 434, 564, 576, 1168, 1543
FauNDI CATATG 1 cut(s) 403
FbaI TGATCA 1 cut(s) 1621
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 5 cut(s) 354, 488, 491, 522, 538
FokI GGATG 2 cut(s) 892, 1410
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 5 cut(s) 354, 488, 491, 522, 538
FspBI CTAG 1 cut(s) 1454
GlaI GCGC 2 cut(s) 171, 1273
GluI GCNGC 5 cut(s) 354, 488, 491, 522, 538
HaeIII GGCC 2 cut(s) 986, 1032
HapII CCGG 1 cut(s) 223
HgaI GACGC 2 cut(s) 53, 345
HhaI GCGC 2 cut(s) 172, 1274
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 8 cut(s) 246, 262, 307, 438, 568, 580, 1172, 1547
Hin6I GCGC 2 cut(s) 170, 1272
HinP1I GCGC 2 cut(s) 170, 1272
HinfI GANTC 2 cut(s) 26, 1358
HpaII CCGG 1 cut(s) 223
HphI GGTGA 1 cut(s) 886
Hpy166II GTNNAC 3 cut(s) 649, 853, 1261
Hpy188III TCNNGA 8 cut(s) 105, 304, 418, 785, 936, 1419, 1454, 1544
Hpy8I GTNNAC 3 cut(s) 649, 853, 1261
Hpy99I CGWCG 3 cut(s) 19, 22, 58
HpyAV CCTTC 5 cut(s) 703, 782, 791, 1388, 1396
HpyCH4III ACNGT 4 cut(s) 186, 330, 624, 813
HpyCH4V TGCA 9 cut(s) 353, 434, 540, 599, 760, 923, 932, 1172, 1518
HpyF10VI GCNNNNNNNGC 4 cut(s) 920, 929, 1100, 1278
HpyF3I CTNAG 5 cut(s) 140, 218, 634, 1440, 1627
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 8 cut(s) 246, 262, 307, 438, 568, 580, 1172, 1547
HspAI GCGC 2 cut(s) 170, 1272
Ksp22I TGATCA 1 cut(s) 1621
Kzo9I GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
LmnI GCTCC 2 cut(s) 727, 864
Lsp1109I GCAGC 4 cut(s) 365, 474, 477, 524
LweI GCATC 3 cut(s) 941, 1103, 1605
MaeI CTAG 1 cut(s) 1454
MaeIII GTNAC 2 cut(s) 41, 603
MalI GATC 7 cut(s) 137, 302, 399, 416, 779, 1552, 1623
MboI GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
MflI RGATCY 2 cut(s) 414, 1550
MhlI GDGCHC 1 cut(s) 462
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 7 cut(s) 101, 149, 220, 870, 954, 976, 1375
MroXI GAANNNNTTC 1 cut(s) 1466
MseI TTAA 8 cut(s) 153, 495, 500, 734, 990, 1004, 1283, 1301
MspA1I CMGCKG 1 cut(s) 1268
MspCI CTTAAG 1 cut(s) 989
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 4 cut(s) 224, 869, 1106, 1355
Mva1269I GAATGC 1 cut(s) 1334
MvaI CCWGG 3 cut(s) 869, 1106, 1355
MwoI GCNNNNNNNGC 4 cut(s) 920, 929, 1100, 1278
NciI CCSGG 1 cut(s) 224
NdeI CATATG 1 cut(s) 403
NdeII GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
NlaIII CATG 8 cut(s) 246, 262, 307, 438, 568, 580, 1172, 1547
NlaIV GGNNCC 2 cut(s) 324, 866
NmuCI GTSAC 2 cut(s) 41, 603
NspI RCATGY 4 cut(s) 246, 438, 568, 1172
PaeI GCATGC 1 cut(s) 568
PagI TCATGA 2 cut(s) 303, 1543
PasI CCCWGGG 1 cut(s) 1105
PctI GAATGC 1 cut(s) 1334
PdmI GAANNNNTTC 1 cut(s) 1466
PfeI GAWTC 2 cut(s) 26, 1358
PflMI CCANNNNNTGG 1 cut(s) 332
PfoI TCCNGGA 1 cut(s) 1353
PkrI GCNGC 5 cut(s) 355, 489, 492, 523, 539
PsiI TTATAA 1 cut(s) 828
Psp6I CCWGG 3 cut(s) 867, 1104, 1353
PspGI CCWGG 3 cut(s) 867, 1104, 1353
PspN4I GGNNCC 2 cut(s) 324, 866
PspPI GGNCC 2 cut(s) 985, 1031
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 2 cut(s) 359, 1195
PsuI RGATCY 2 cut(s) 414, 1550
RsaI GTAC 6 cut(s) 80, 183, 274, 907, 1167, 1242
RsaNI GTAC 6 cut(s) 79, 182, 273, 906, 1166, 1241
SaqAI TTAA 8 cut(s) 153, 495, 500, 734, 990, 1004, 1283, 1301
SatI GCNGC 5 cut(s) 354, 488, 491, 522, 538
Sau3AI GATC 7 cut(s) 135, 300, 397, 414, 777, 1550, 1621
Sau96I GGNCC 2 cut(s) 985, 1031
ScaI AGTACT 1 cut(s) 1242
ScrFI CCNGG 4 cut(s) 224, 869, 1106, 1355
SduI GDGCHC 1 cut(s) 462
SfaNI GCATC 3 cut(s) 941, 1103, 1605
SfcI CTRYAG 1 cut(s) 351
SmlI CTYRAG 2 cut(s) 385, 989
SmoI CTYRAG 2 cut(s) 385, 989
SphI GCATGC 1 cut(s) 568
SsiI CCGC 5 cut(s) 191, 429, 522, 1266, 1612
SspI AATATT 2 cut(s) 1320, 1449
SspMI CTAG 1 cut(s) 1454
StyD4I CCNGG 4 cut(s) 222, 867, 1104, 1353
TaaI ACNGT 4 cut(s) 186, 330, 624, 813
TatI WGTACW 3 cut(s) 181, 1165, 1240
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 26, 1358
Tru1I TTAA 8 cut(s) 153, 495, 500, 734, 990, 1004, 1283, 1301
Tru9I TTAA 8 cut(s) 153, 495, 500, 734, 990, 1004, 1283, 1301
TscAI CASTG 1 cut(s) 818
TseFI GTSAC 2 cut(s) 41, 603
TseI GCWGC 4 cut(s) 353, 487, 490, 537
Tsp45I GTSAC 2 cut(s) 41, 603
TspDTI ATGAA 7 cut(s) 109, 275, 320, 1157, 1287, 1327, 1478
TspGWI ACGGA 1 cut(s) 1607
TspRI CASTG 1 cut(s) 818
Van91I CCANNNNNTGG 1 cut(s) 332
Vha464I CTTAAG 1 cut(s) 989
XagI CCTNNNNNAGG 1 cut(s) 980
XapI RAATTY 6 cut(s) 111, 145, 308, 796, 1294, 1465
XbaI TCTAGA 1 cut(s) 1453
XceI RCATGY 4 cut(s) 246, 438, 568, 1172
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XmiI GTMKAC 1 cut(s) 648
XmnI GAANNNNTTC 1 cut(s) 1466
XspI CTAG 1 cut(s) 1454
ZrmI AGTACT 1 cut(s) 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.