Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005423

Physical Location & Seq

Genomic Coordinates Forward (+)

3624066 .. 3630688

Gene Structure 6623 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1042200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGTCAAATTCTTCGCCGGTTGGATCCCAATCTCAACAATTCAGTGACAGCAGCTTCTCTCGCACCTTCAAGTACTTACTCGCTACCCAATTCTTGTCGAGGGGAATCCCATTCATATTCAATGCGTGGATCGTCAGACACCTCTCAGTCGAGGACTTTGCGCTTTATGGAGTACAATTTCCTCTTTTTGTCACCTGTGTATTGTTTCTGAGTCGGGAGGGAGTTCGGCGAGCATGCCTGCGCGCAGACATGAAACGTGATGGTGCGTCGACAGAAGAAAATATAGCAAAGATAATGAAATTTGCTTGGTTGATTCCCCCATGTGGAATTCTTCTTACTGTTGCAGCGTGCACTGTTGTCTTCTGGAATAAAGACTTAAGCTTTTCTAATCCATATGGACAAGCGATCTTAATTAATGGCCTTGCATGTATTTTTGAACTTTTGGCAGAACCCTTGTATATCCTTTCCCAGAACTTGCTTTTGCTTAAATTAAGGCTAGTAGTTGAAACAGTTGCCACTCTTTTCCGGTGCATAACGATGTTCATTCTCATTGTCAAGCAAACTGACATGGAGAAAGCAATTGTATTTACATTGTCACAAACTGCCTATGGAGTGTGCTTGTTCCTAGGTTATTGGGTCTACTTCCTCTTTTTTGCTGCATGTAGAAGTACTGCTCTTTTCCCCTTCAGTGTAAAGAATAAGAAGGATTATGATAGGGAGCTGTCAGACATGTGTAAGCTGTTTACGCTTCAATCCTTTGTGAAGTTGATCCTTCAAGAAGGACAAAGTCTTATCCTTTTATGGTGGGCGACATATTATAACCAAGCTGTGTATGGGCTTGTGGATAAATTAGGGAGCCTAGTGGTCAGGATGGTGTTTCTTCCTTTTGAAGAAAGTTCCTATGCTACATTTGCAAGGTCTGCATCAGGAAAAGACCCTGATAAAGACAGGCGGTTAGGAAGTAGCCTTACAGAGGCCCTCAAGTTGGTTTTGTTAATAGGTCTTGTATTCATGGTGTTTGGCCCGAGTTATTCTTATTCTCTCATCAGATTGTTATACGGTCGGAACTGGAGTGATGGGGAAGCATCATCAGCCCTTCGATATTATTGCTTTTATATAATAGTTTTGGCGATGAATGGAACTTCTGAAGCATTTCTACATGCCGTTGCAAAGGAGAAGCAACTCGTACAGTCAAATCTGTCGTCGTTTCTGTTCGCATTCATACATATAGTGCTGAATATCCTGCTGATAAACTCAGCTGGTGCAGTCGGCTTGATTTTGGCAAATTCATTAAACATGATTCTGAGAATCATCTACTCGGGGATATTCATCAAGCATCATTTCCAGGATTCTTCCACGTTTTCTTTTTACAGATGTTTACCTTCAGGATGGAAGGTGCTATTGTTTTCGGGAATGACAACTACTATTTCTGAGAGAGTATTCCTGGACCGGGAGAATTTCTTGCCGACCTTTTTGGTTCATTTTTCCATTGGTGTTGCTTGCTTCTGCATGTCGTCGTTTGTTATATACCGCCGTGAGAGATCCTTCATCAACAAAATCATCCGCTTCCGTGATCACACAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006810 GO:0006869 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0016020 GO:0016043 GO:0031984 GO:0033036 GO:0034203 GO:0034204 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046836 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0097035 GO:0098827 GO:1901264

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rft-1

Protein Analysis

529

Amino Acids

60.07

Weight (kDa)

9.1

Isoelectric Point (pI)

48.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rft-1	PF04506	22 - 521	9.6e-137	Rft protein
Polysacc_synt	PF01943	25 - 305	3.5e-08	Polysaccharide biosynthesis protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07630 AT5G07630
fragaria_vesca	FvH4_3g41750 FvH4_3g41750 FvH4_3g41750 FvH4_3g41750 FvH4_3g41750 FvH4_3g41750 FvH4_3g41750
malus_domestica	MD11G1042200.v1.1
prunus_persica	Prupe.6G033300_v2.0.a1 Prupe.6G033300_v2.0.a1 Prupe.6G033300_v2.0.a1 Prupe.6G033300_v2.0.a1 Prupe.6G033300_v2.0.a1
pyrus_communis	pycom11g03630
rosa_chinensis	RchiOBHm_Chr5g0074831
rosa_laevigata	RLG00000036509
rosa_multiflora	Rmu_sc0000909.1_g000001 Rmu_sc0003945.1_g000001 Rmu_sc0005365.1_g000010
rosa_roxburghii	Rroxscaffold_1G00006740
rosa_rugosa	Rorug05G0434700 Rorug05G0434800
rosa_samantha	Rh5AG492100 Rh5BG513500 Rh5CG537200 Rh5DG525600
rosa_wichuraiana	Rw5G045590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	819
AccI	GTMKAC	2 cut(s)	269, 639
AccII	CGCG	1 cut(s)	243
AciI	CCGC	3 cut(s)	952, 1531, 1564
AclWI	GGATC	5 cut(s)	18, 31, 137, 763, 1536
AcsI	RAATTY	5 cut(s)	7, 299, 327, 1285, 1456
AcuI	CTGAAG	3 cut(s)	670, 1167, 1368
AfaI	GTAC	4 cut(s)	74, 174, 670, 1188
AfiI	CCNNNNNNNGG	4 cut(s)	323, 973, 985, 1450
AflII	CTTAAG	1 cut(s)	376
AflIII	ACRYGT	1 cut(s)	729
AgsI	TTSAA	7 cut(s)	70, 121, 437, 506, 752, 776, 890
AjnI	CCWGG	2 cut(s)	1344, 1443
AluBI	AGCT	6 cut(s)	54, 381, 721, 739, 827, 1259
AluI	AGCT	6 cut(s)	54, 381, 721, 739, 827, 1259
Alw21I	GWGCWC	1 cut(s)	353
Alw44I	GTGCAC	1 cut(s)	349
AlwI	GGATC	5 cut(s)	18, 31, 137, 763, 1536
Ama87I	CYCGRG	2 cut(s)	1024, 1318
AoxI	GGCC	3 cut(s)	418, 975, 1021
ApaLI	GTGCAC	1 cut(s)	349
ApeKI	GCWGC	3 cut(s)	51, 344, 656
ApoI	RAATTY	5 cut(s)	7, 299, 327, 1285, 1456
ArsI	GACNNNNNNTTYG	2 cut(s)	1187, 1219
AseI	ATTAAT	1 cut(s)	414
Asp700I	GAANNNNTTC	1 cut(s)	1152
AspA2I	CCTAGG	1 cut(s)	625
AspLEI	GCGC	3 cut(s)	163, 243, 245
AspS9I	GGNCC	3 cut(s)	976, 1022, 1447
AsuC2I	CCSGG	1 cut(s)	1451
AsuHPI	GGTGA	1 cut(s)	184
AvaI	CYCGRG	2 cut(s)	1024, 1318
AvaII	GGWCC	1 cut(s)	1447
AvrII	CCTAGG	1 cut(s)	625
BaeGI	GKGCMC	1 cut(s)	353
BamHI	GGATCC	1 cut(s)	23
BarI	GAAGNNNNNNTAC	4 cut(s)	952, 984, 1140, 1172
BbsI	GAAGAC	1 cut(s)	352
Bbv12I	GWGCWC	1 cut(s)	353
BbvI	GCAGC	3 cut(s)	63, 356, 643
BccI	CCATC	4 cut(s)	254, 865, 1070, 1383
BceAI	ACGGC	2 cut(s)	1148, 1518
BcgI	CGANNNNNNTGC	4 cut(s)	140, 174, 1089, 1123
BciT130I	CCWGG	2 cut(s)	1346, 1445
BclI	TGATCA	1 cut(s)	1573
BcnI	CCSGG	1 cut(s)	1451
BfaI	CTAG	3 cut(s)	497, 626, 860
BfrI	CTTAAG	1 cut(s)	376
BisI	GCNGC	3 cut(s)	52, 345, 657
BlnI	CCTAGG	1 cut(s)	625
BlsI	GCNGC	3 cut(s)	53, 346, 658
BmcAI	AGTACT	2 cut(s)	74, 670
Bme1390I	CCNGG	3 cut(s)	1346, 1445, 1451
Bme18I	GGWCC	1 cut(s)	1447
BmeT110I	CYCGRG	2 cut(s)	1024, 1318
BmgT120I	GGNCC	3 cut(s)	976, 1022, 1447
BmiI	GGNNCC	2 cut(s)	25, 857
BmrFI	CCNGG	3 cut(s)	1346, 1445, 1451
BmsI	GCATC	3 cut(s)	932, 1094, 1345
BpiI	GAAGAC	1 cut(s)	352
BpmI	CTGGAG	1 cut(s)	1090
BpuEI	CTTGAG	1 cut(s)	965
BpuMI	CCSGG	1 cut(s)	1451
BsaBI	GATNNNNATC	2 cut(s)	28, 1328
BsaJI	CCNNGG	1 cut(s)	625
BsaWI	WCCGGW	1 cut(s)	525
Bsc4I	CCNNNNNNNGG	4 cut(s)	323, 973, 985, 1450
Bse118I	RCCGGY	1 cut(s)	16
Bse1I	ACTGG	1 cut(s)	1073
Bse8I	GATNNNNATC	2 cut(s)	28, 1328
BseBI	CCWGG	2 cut(s)	1346, 1445
BseDI	CCNNGG	1 cut(s)	625
BseGI	GGATG	3 cut(s)	876, 1394, 1560
BseJI	GATNNNNATC	2 cut(s)	28, 1328
BseLI	CCNNNNNNNGG	4 cut(s)	323, 973, 985, 1450
BseMII	CTCAG	5 cut(s)	159, 200, 1269, 1295, 1422
BseNI	ACTGG	1 cut(s)	1073
BsePI	GCGCGC	1 cut(s)	241
BseSI	GKGCMC	1 cut(s)	353
BseXI	GCAGC	3 cut(s)	63, 356, 643
BsgI	GTGCAG	1 cut(s)	1284
Bsh1236I	CGCG	1 cut(s)	243
Bsh1285I	CGRYCG	1 cut(s)	1063
BshFI	GGCC	3 cut(s)	420, 977, 1023
BsiEI	CGRYCG	1 cut(s)	1063
BsiHKAI	GWGCWC	1 cut(s)	353
BsiHKCI	CYCGRG	2 cut(s)	1024, 1318
BsiSI	CCGG	3 cut(s)	17, 526, 1450
BslI	CCNNNNNNNGG	4 cut(s)	323, 973, 985, 1450
BsmI	GAATGC	1 cut(s)	1217
BsnI	GGCC	3 cut(s)	420, 977, 1023
BsoBI	CYCGRG	2 cut(s)	1024, 1318
Bsp1286I	GDGCHC	1 cut(s)	353
Bsp143I	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
BspACI	CCGC	3 cut(s)	952, 1531, 1564
BspANI	GGCC	3 cut(s)	420, 977, 1023
BspCNI	CTCAG	5 cut(s)	158, 201, 1268, 1296, 1423
BspFNI	CGCG	1 cut(s)	243
BspLI	GGNNCC	2 cut(s)	25, 857
BspPI	GGATC	5 cut(s)	18, 31, 137, 763, 1536
BspTI	CTTAAG	1 cut(s)	376
BsrFI	RCCGGY	1 cut(s)	16
BsrI	ACTGG	1 cut(s)	1073
BssAI	RCCGGY	1 cut(s)	16
BssECI	CCNNGG	1 cut(s)	625
BssHII	GCGCGC	1 cut(s)	241
BssMI	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
BssT1I	CCWWGG	1 cut(s)	625
Bst2UI	CCWGG	2 cut(s)	1346, 1445
Bst4CI	ACNGT	5 cut(s)	340, 355, 511, 1061, 1191
BstAFI	CTTAAG	1 cut(s)	376
BstAPI	GCANNNNNTGC	1 cut(s)	920
BstC8I	GCNNGC	6 cut(s)	231, 235, 239, 243, 349, 1501
BstDEI	CTNAG	5 cut(s)	145, 209, 1255, 1304, 1431
BstENI	CCTNNNNNAGG	1 cut(s)	971
BstF5I	GGATG	3 cut(s)	876, 1394, 1560
BstFNI	CGCG	1 cut(s)	243
BstHHI	GCGC	3 cut(s)	163, 243, 245
BstKTI	GATC	6 cut(s)	26, 132, 408, 771, 1544, 1576
BstMBI	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
BstMCI	CGRYCG	1 cut(s)	1063
BstMWI	GCNNNNNNNGC	5 cut(s)	60, 745, 911, 920, 1091
BstNI	CCWGG	2 cut(s)	1346, 1445
BstNSI	RCATGY	6 cut(s)	237, 429, 663, 733, 1163, 1513
BstSCI	CCNGG	3 cut(s)	1344, 1443, 1449
BstSLI	GKGCMC	1 cut(s)	353
BstUI	CGCG	1 cut(s)	243
BstV1I	GCAGC	3 cut(s)	63, 356, 643
BstV2I	GAAGAC	1 cut(s)	352
BstX2I	RGATCY	2 cut(s)	23, 1541
BstYI	RGATCY	2 cut(s)	23, 1541
BsuRI	GGCC	3 cut(s)	420, 977, 1023
BtgZI	GCGATG	1 cut(s)	1145
BtsCI	GGATG	3 cut(s)	876, 1394, 1560
BtsIMutI	CAGTG	3 cut(s)	49, 351, 694
Cac8I	GCNNGC	6 cut(s)	231, 235, 239, 243, 349, 1501
CfoI	GCGC	3 cut(s)	163, 243, 245
Cfr10I	RCCGGY	1 cut(s)	16
Cfr13I	GGNCC	3 cut(s)	976, 1022, 1447
CseI	GACGC	1 cut(s)	255
Csp6I	GTAC	4 cut(s)	73, 173, 669, 1187
CviQI	GTAC	4 cut(s)	73, 173, 669, 1187
DdeI	CTNAG	5 cut(s)	145, 209, 1255, 1304, 1431
DpnI	GATC	6 cut(s)	25, 131, 407, 770, 1543, 1575
DpnII	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
Eco130I	CCWWGG	1 cut(s)	625
Eco47I	GGWCC	1 cut(s)	1447
Eco57I	CTGAAG	3 cut(s)	670, 1167, 1368
Eco88I	CYCGRG	2 cut(s)	1024, 1318
EcoNI	CCTNNNNNAGG	1 cut(s)	971
EcoO109I	RGGNCCY	1 cut(s)	976
EcoRI	GAATTC	1 cut(s)	327
EcoRII	CCWGG	2 cut(s)	1344, 1443
EcoT14I	CCWWGG	1 cut(s)	625
ErhI	CCWWGG	1 cut(s)	625
FauNDI	CATATG	1 cut(s)	394
FbaI	TGATCA	1 cut(s)	1573
FblI	GTMKAC	2 cut(s)	269, 639
Fnu4HI	GCNGC	3 cut(s)	52, 345, 657
FokI	GGATG	3 cut(s)	883, 1401, 1547
Fsp4HI	GCNGC	3 cut(s)	52, 345, 657
FspBI	CTAG	3 cut(s)	497, 626, 860
GlaI	GCGC	3 cut(s)	162, 242, 244
GluI	GCNGC	3 cut(s)	52, 345, 657
GsuI	CTGGAG	1 cut(s)	1090
HaeIII	GGCC	3 cut(s)	420, 977, 1023
HapII	CCGG	3 cut(s)	17, 526, 1450
HgaI	GACGC	1 cut(s)	255
HhaI	GCGC	3 cut(s)	163, 243, 245
Hin6I	GCGC	3 cut(s)	161, 241, 243
HinP1I	GCGC	3 cut(s)	161, 241, 243
HincII	GTYRAC	1 cut(s)	270
HindII	GTYRAC	1 cut(s)	270
HindIII	AAGCTT	1 cut(s)	379
HinfI	GANTC	6 cut(s)	105, 211, 313, 1300, 1308, 1349
HpaII	CCGG	3 cut(s)	17, 526, 1450
HphI	GGTGA	1 cut(s)	184
Hpy166II	GTNNAC	5 cut(s)	270, 351, 640, 744, 1379
Hpy188I	TCNGA	8 cut(s)	137, 210, 727, 1049, 1065, 1147, 1305, 1432
Hpy188III	TCNNGA	7 cut(s)	215, 364, 776, 868, 927, 1386, 1410
Hpy8I	GTNNAC	5 cut(s)	270, 351, 640, 744, 1379
Hpy99I	CGWCG	3 cut(s)	271, 1207, 1519
HpyAV	CCTTC	9 cut(s)	76, 694, 697, 773, 782, 1106, 1387, 1392, 1555
HpyCH4III	ACNGT	5 cut(s)	340, 355, 511, 1061, 1191
HpyCH4IV	ACGT	2 cut(s)	256, 1358
HpyF10VI	GCNNNNNNNGC	5 cut(s)	60, 745, 911, 920, 1091
HpyF3I	CTNAG	5 cut(s)	145, 209, 1255, 1304, 1431
HpySE526I	ACGT	2 cut(s)	256, 1358
HspAI	GCGC	3 cut(s)	161, 241, 243
Ksp22I	TGATCA	1 cut(s)	1573
Kzo9I	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
LmnI	GCTCC	2 cut(s)	718, 855
Lsp1109I	GCAGC	3 cut(s)	63, 356, 643
LweI	GCATC	3 cut(s)	932, 1094, 1345
MaeI	CTAG	3 cut(s)	497, 626, 860
MaeII	ACGT	2 cut(s)	256, 1358
MaeIII	GTNAC	3 cut(s)	44, 190, 594
MalI	GATC	6 cut(s)	25, 131, 407, 770, 1543, 1575
MboI	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
MboII	GAAGA	7 cut(s)	3, 287, 323, 352, 872, 902, 1344
MfeI	CAATTG	1 cut(s)	579
MflI	RGATCY	2 cut(s)	23, 1541
MhlI	GDGCHC	1 cut(s)	353
MlyI	GAGTC	1 cut(s)	220
MmeI	TCCRAC	1 cut(s)	1043
MnlI	CCTC	8 cut(s)	93, 145, 152, 192, 211, 656, 967, 989
MroXI	GAANNNNTTC	1 cut(s)	1152
MseI	TTAA	7 cut(s)	377, 410, 414, 486, 491, 995, 1292
MslI	CAYNNNNRTG	1 cut(s)	536
MspA1I	CMGCKG	1 cut(s)	1259
MspCI	CTTAAG	1 cut(s)	376
MspI	CCGG	3 cut(s)	17, 526, 1450
MspR9I	CCNGG	3 cut(s)	1346, 1445, 1451
MunI	CAATTG	1 cut(s)	579
Mva1269I	GAATGC	1 cut(s)	1217
MvaI	CCWGG	2 cut(s)	1346, 1445
MvnI	CGCG	1 cut(s)	243
MwoI	GCNNNNNNNGC	5 cut(s)	60, 745, 911, 920, 1091
NciI	CCSGG	1 cut(s)	1451
NdeI	CATATG	1 cut(s)	394
NdeII	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
NlaIV	GGNNCC	2 cut(s)	25, 857
NmuCI	GTSAC	3 cut(s)	44, 190, 594
NspI	RCATGY	6 cut(s)	237, 429, 663, 733, 1163, 1513
PacI	TTAATTAA	1 cut(s)	414
PaeI	GCATGC	1 cut(s)	237
PauI	GCGCGC	1 cut(s)	241
PciI	ACATGT	1 cut(s)	729
PctI	GAATGC	1 cut(s)	1217
PdmI	GAANNNNTTC	1 cut(s)	1152
PfeI	GAWTC	5 cut(s)	105, 313, 1300, 1308, 1349
PflFI	GACNNNGTC	1 cut(s)	786
PfoI	TCCNGGA	2 cut(s)	1344, 1443
PkrI	GCNGC	3 cut(s)	53, 346, 658
PleI	GAGTC	1 cut(s)	219
PpsI	GAGTC	1 cut(s)	219
PscI	ACATGT	1 cut(s)	729
PshBI	ATTAAT	1 cut(s)	414
PsiI	TTATAA	1 cut(s)	819
Psp6I	CCWGG	2 cut(s)	1344, 1443
PspGI	CCWGG	2 cut(s)	1344, 1443
PspN4I	GGNNCC	2 cut(s)	25, 857
PspPI	GGNCC	3 cut(s)	976, 1022, 1447
PsuI	RGATCY	2 cut(s)	23, 1541
PsyI	GACNNNGTC	1 cut(s)	786
PteI	GCGCGC	1 cut(s)	241
PvuII	CAGCTG	1 cut(s)	1259
RsaI	GTAC	4 cut(s)	74, 174, 670, 1188
RsaNI	GTAC	4 cut(s)	73, 173, 669, 1187
RseI	CAYNNNNRTG	1 cut(s)	536
SalI	GTCGAC	1 cut(s)	268
SaqAI	TTAA	7 cut(s)	377, 410, 414, 486, 491, 995, 1292
SatI	GCNGC	3 cut(s)	52, 345, 657
Sau3AI	GATC	6 cut(s)	23, 129, 405, 768, 1541, 1573
Sau96I	GGNCC	3 cut(s)	976, 1022, 1447
ScaI	AGTACT	2 cut(s)	74, 670
SchI	GAGTC	1 cut(s)	220
ScrFI	CCNGG	3 cut(s)	1346, 1445, 1451
SduI	GDGCHC	1 cut(s)	353
SfaNI	GCATC	3 cut(s)	932, 1094, 1345
SinI	GGWCC	1 cut(s)	1447
SmiMI	CAYNNNNRTG	1 cut(s)	536
SmlI	CTYRAG	2 cut(s)	376, 980
SmoI	CTYRAG	2 cut(s)	376, 980
SphI	GCATGC	1 cut(s)	237
SsiI	CCGC	3 cut(s)	952, 1531, 1564
SspMI	CTAG	3 cut(s)	497, 626, 860
StyD4I	CCNGG	3 cut(s)	1344, 1443, 1449
StyI	CCWWGG	1 cut(s)	625
TaaI	ACNGT	5 cut(s)	340, 355, 511, 1061, 1191
TaiI	ACGT	2 cut(s)	259, 1361
TaqI	TCGA	4 cut(s)	98, 150, 269, 1099
TatI	WGTACW	3 cut(s)	72, 172, 668
TfiI	GAWTC	5 cut(s)	105, 313, 1300, 1308, 1349
Tru1I	TTAA	7 cut(s)	377, 410, 414, 486, 491, 995, 1292
Tru9I	TTAA	7 cut(s)	377, 410, 414, 486, 491, 995, 1292
TscAI	CASTG	3 cut(s)	49, 358, 694
TseFI	GTSAC	3 cut(s)	44, 190, 594
TseI	GCWGC	3 cut(s)	51, 344, 656
Tsp45I	GTSAC	3 cut(s)	44, 190, 594
TspGWI	ACGGA	1 cut(s)	1559
TspRI	CASTG	3 cut(s)	49, 358, 694
Tth111I	GACNNNGTC	1 cut(s)	786
Vha464I	CTTAAG	1 cut(s)	376
VneI	GTGCAC	1 cut(s)	349
VpaK11BI	GGWCC	1 cut(s)	1447
VspI	ATTAAT	1 cut(s)	414
XagI	CCTNNNNNAGG	1 cut(s)	971
XapI	RAATTY	5 cut(s)	7, 299, 327, 1285, 1456
XceI	RCATGY	6 cut(s)	237, 429, 663, 733, 1163, 1513
XcmI	CCANNNNNNNNNTGG	1 cut(s)	830
XmaJI	CCTAGG	1 cut(s)	625
XmiI	GTMKAC	2 cut(s)	269, 639
XmnI	GAANNNNTTC	1 cut(s)	1152
XspI	CTAG	3 cut(s)	497, 626, 860
ZrmI	AGTACT	2 cut(s)	74, 670

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.