FvH4_4g00460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014708
Physical Location & Seq
Forward (+)
437715 .. 439433
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00460.t1

Sequence Viewer

Length: 1023 bp
ATGTTTCAGAGGGGATTTGCAGCAGCAGCAGCTGCAAGGTTAAGAGGAGGAATTGGATTAGGTGCTTTAAGGGTTGATCATACGAAGAAGGCAATGCAAATTAGCAACGGTGGTCATGGTGGATTCATCATGAGAAGAAGGTATTCTTCCGACGTCTCCTCCAAATCCTTACACTTGTGTTTCTGTGAGCATATCAGTTCAGAACAACACCGCACACGTGATGTTGTTCGCTCCATCAAATTGAGCGATTTGTTATCACCACCACCACCACCAACAACAACCACAACTAGGTTTGAGCAAGAAGCATATCATGATTCTGAAAACCGTCATAGCGACCCCTTCCCGTGGGGTTGCGGTCGCTTCGGGTCCCAAATTGTCTTCGCCGGTGGCTCCCTCAACAACATTTACACTGGTGTTAGAGGAGAAATTGTTGCCTTTGATCCTAATTCTTTCACATTCACGGCCCCGAAATTCACACCACCACGTAAACATCATTATCGATTCCCAACTGCGTTGTTTGAGGTGCAAGGAAAGCTCTACTGTCTTGACACTAGAAACCAGCAATATCACAGTACCGTTCCCGATTTCCAGATGATGGGTCACTATCACCACTACACCGATGCTCCTCCTGCTTTTGCAGTACTTGAAACCGAAGGTGATGCAGAGTTTCAGGTCAAATGGCTGCCTCTAGAGCCGCCTCCTCTGGCTATACTCTACGACAATAAATCCGAAAAGCTCAAGGAGTTGATGTATTGTGCTGCCGAGTCCAAAATCTTAGTCTGGAATCCAAACACGGCCGGGGTTCACTACTTTGACGTGAGCCGACCCGAAAAGGGATGGACAGAGTTGCACTCTCCTCTAGCAACCAGCCTTCCCTTTGTTAAGGGTTGTTGGGGTGACACTGCCTATCATCACGGTGTTGGAGCAGGAACCCTAATATTTACCTTCGACTGTACACCTAAACTTAGTGATTGTTCTATATTTTACGGGGCTTGGAGTGTACAAGTTTTGCCCGTAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

38.06

Weight (kDa)

7.69

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 94 - 294 4.7e-09 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 595
AciI CCGC 3 cut(s) 211, 354, 695
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 1 cut(s) 470
AcvI CACGTG 1 cut(s) 218
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 4 cut(s) 574, 642, 955, 1002
AfiI CCNNNNNNNGG 4 cut(s) 288, 345, 595, 833
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 1 cut(s) 647
AjiI CACGTC 1 cut(s) 817
AluBI AGCT 3 cut(s) 32, 535, 736
AluI AGCT 3 cut(s) 32, 535, 736
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 1 cut(s) 434
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 2 cut(s) 462, 795
ApeKI GCWGC 7 cut(s) 20, 23, 26, 29, 32, 682, 758
ApoI RAATTY 1 cut(s) 470
ArsI GACNNNNNNTTYG 2 cut(s) 762, 794
Asp700I GAANNNNTTC 1 cut(s) 142
AspS9I GGNCC 2 cut(s) 366, 463
AsuC2I CCSGG 1 cut(s) 799
AsuHPI GGTGA 4 cut(s) 249, 599, 668, 908
AvaII GGWCC 1 cut(s) 366
BbrPI CACGTG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 7 cut(s) 19, 32, 35, 38, 41, 669, 745
BccI CCATC 3 cut(s) 242, 589, 831
BceAI ACGGC 2 cut(s) 477, 810
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 1 cut(s) 799
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 4 cut(s) 288, 552, 689, 860
BisI GCNGC 8 cut(s) 21, 24, 27, 30, 33, 683, 695, 759
BlsI GCNGC 8 cut(s) 22, 25, 28, 31, 34, 684, 696, 760
BmcAI AGTACT 1 cut(s) 642
Bme1390I CCNGG 1 cut(s) 799
Bme18I GGWCC 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 817
BmgT120I GGNCC 2 cut(s) 366, 463
BmiI GGNNCC 5 cut(s) 367, 368, 391, 465, 931
BmrFI CCNGG 1 cut(s) 799
BmsI GCATC 2 cut(s) 610, 649
BpiI GAAGAC 1 cut(s) 370
BplI GAGNNNNNCTC 2 cut(s) 836, 868
BpuEI CTTGAG 1 cut(s) 722
BpuMI CCSGG 1 cut(s) 799
Bsa29I ATCGAT 1 cut(s) 499
BsaAI YACGTR 2 cut(s) 218, 485
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 344, 798
BsaXI ACNNNNNCTCC 8 cut(s) 143, 173, 414, 444, 607, 637, 734, 764
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 345, 595, 833
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 415
Bse3DI GCAATG 1 cut(s) 99
BseCI ATCGAT 1 cut(s) 499
BseDI CCNNGG 2 cut(s) 344, 798
BseGI GGATG 1 cut(s) 842
BseLI CCNNNNNNNGG 4 cut(s) 288, 345, 595, 833
BseMI GCAATG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 415
BseRI GAGGAG 6 cut(s) 60, 148, 435, 615, 690, 846
BseX3I CGGCCG 1 cut(s) 795
BseXI GCAGC 7 cut(s) 19, 32, 35, 38, 41, 669, 745
Bsh1285I CGRYCG 2 cut(s) 358, 798
BshFI GGCC 2 cut(s) 464, 797
BshVI ATCGAT 1 cut(s) 499
BsiEI CGRYCG 2 cut(s) 358, 798
BsiSI CCGG 2 cut(s) 384, 798
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 4 cut(s) 288, 345, 595, 833
BsmAI GTCTC 1 cut(s) 160
BsmBI CGTCTC 1 cut(s) 160
BsmFI GGGAC 1 cut(s) 352
BsnI GGCC 2 cut(s) 464, 797
Bsp1407I TGTACA 2 cut(s) 953, 1000
Bsp143I GATC 2 cut(s) 76, 439
BspACI CCGC 3 cut(s) 211, 354, 695
BspANI GGCC 2 cut(s) 464, 797
BspDI ATCGAT 1 cut(s) 499
BspHI TCATGA 2 cut(s) 129, 310
BspLI GGNNCC 5 cut(s) 367, 368, 391, 465, 931
BspPI GGATC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 383
BsrGI TGTACA 2 cut(s) 953, 1000
BsrI ACTGG 1 cut(s) 415
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 2 cut(s) 344, 798
BssMI GATC 2 cut(s) 76, 439
BssNI GRCGYC 1 cut(s) 153
Bst4CI ACNGT 7 cut(s) 110, 326, 542, 572, 577, 917, 953
BstACI GRCGYC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 32
BstAUI TGTACA 2 cut(s) 953, 1000
BstBAI YACGTR 2 cut(s) 218, 485
BstDEI CTNAG 2 cut(s) 775, 965
BstDSI CCRYGG 1 cut(s) 344
BstF5I GGATG 1 cut(s) 842
BstKTI GATC 2 cut(s) 79, 442
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 2 cut(s) 76, 439
BstMCI CGRYCG 2 cut(s) 358, 798
BstMWI GCNNNNNNNGC 6 cut(s) 26, 29, 32, 532, 629, 691
BstSCI CCNGG 1 cut(s) 797
BstV1I GCAGC 7 cut(s) 19, 32, 35, 38, 41, 669, 745
BstV2I GAAGAC 1 cut(s) 370
BstZI CGGCCG 1 cut(s) 795
Bsu15I ATCGAT 1 cut(s) 499
BsuRI GGCC 2 cut(s) 464, 797
BsuTUI ATCGAT 1 cut(s) 499
BtgI CCRYGG 1 cut(s) 344
BtrI CACGTC 1 cut(s) 817
BtsCI GGATG 1 cut(s) 842
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 2 cut(s) 408, 900
CaiI CAGNNNCTG 1 cut(s) 32
CciI TCATGA 2 cut(s) 129, 310
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 366, 463
ClaI ATCGAT 1 cut(s) 499
Csp6I GTAC 4 cut(s) 573, 641, 954, 1001
CviAII CATG 3 cut(s) 116, 130, 311
CviQI GTAC 4 cut(s) 573, 641, 954, 1001
DdeI CTNAG 2 cut(s) 775, 965
DpnI GATC 2 cut(s) 78, 441
DpnII GATC 2 cut(s) 76, 439
EaeI YGGCCR 1 cut(s) 795
EagI CGGCCG 1 cut(s) 795
EclXI CGGCCG 1 cut(s) 795
Eco47I GGWCC 1 cut(s) 366
Eco52I CGGCCG 1 cut(s) 795
Eco72I CACGTG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 3 cut(s) 119, 133, 314
FaiI YATR 9 cut(s) 81, 117, 131, 192, 307, 312, 330, 710, 980
FalI AAGNNNNNCTT 2 cut(s) 130, 162
FaqI GGGAC 1 cut(s) 352
FatI CATG 3 cut(s) 115, 129, 310
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 8 cut(s) 21, 24, 27, 30, 33, 683, 695, 759
FokI GGATG 1 cut(s) 849
Fsp4HI GCNGC 8 cut(s) 21, 24, 27, 30, 33, 683, 695, 759
FspBI CTAG 4 cut(s) 288, 552, 689, 860
GluI GCNGC 8 cut(s) 21, 24, 27, 30, 33, 683, 695, 759
HaeIII GGCC 2 cut(s) 464, 797
HapII CCGG 2 cut(s) 384, 798
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 3 cut(s) 119, 133, 314
HinfI GANTC 5 cut(s) 123, 314, 501, 764, 784
HpaII CCGG 2 cut(s) 384, 798
HphI GGTGA 4 cut(s) 249, 599, 668, 908
Hpy166II GTNNAC 4 cut(s) 488, 805, 956, 1001
Hpy188I TCNGA 5 cut(s) 9, 151, 202, 319, 730
Hpy188III TCNNGA 7 cut(s) 130, 311, 545, 581, 589, 689, 781
Hpy8I GTNNAC 4 cut(s) 488, 805, 956, 1001
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 6 cut(s) 82, 132, 349, 647, 881, 955
HpyCH4III ACNGT 7 cut(s) 110, 326, 542, 572, 577, 917, 953
HpyCH4IV ACGT 4 cut(s) 153, 217, 484, 816
HpyCH4V TGCA 7 cut(s) 20, 35, 97, 526, 638, 662, 850
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 29, 32, 532, 629, 691
HpyF3I CTNAG 2 cut(s) 775, 965
HpySE526I ACGT 4 cut(s) 153, 217, 484, 816
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 3 cut(s) 119, 133, 314
KflI GGGWCCC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 2 cut(s) 76, 439
LmnI GCTCC 4 cut(s) 236, 395, 628, 923
Lsp1109I GCAGC 7 cut(s) 19, 32, 35, 38, 41, 669, 745
LweI GCATC 2 cut(s) 610, 649
MaeI CTAG 4 cut(s) 288, 552, 689, 860
MaeII ACGT 4 cut(s) 153, 217, 484, 816
MaeIII GTNAC 2 cut(s) 599, 896
MalI GATC 2 cut(s) 78, 441
MboI GATC 2 cut(s) 76, 439
MboII GAAGA 4 cut(s) 97, 138, 147, 370
MluCI AATT 8 cut(s) 51, 99, 239, 372, 426, 445, 470, 1017
MlyI GAGTC 1 cut(s) 773
MmeI TCCRAC 2 cut(s) 174, 901
MroXI GAANNNNTTC 1 cut(s) 142
MseI TTAA 3 cut(s) 41, 68, 882
MslI CAYNNNNRTG 1 cut(s) 915
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 2 cut(s) 384, 798
MspR9I CCNGG 1 cut(s) 799
MwoI GCNNNNNNNGC 6 cut(s) 26, 29, 32, 532, 629, 691
NciI CCSGG 1 cut(s) 799
NdeII GATC 2 cut(s) 76, 439
NlaIII CATG 3 cut(s) 119, 133, 314
NlaIV GGNNCC 5 cut(s) 367, 368, 391, 465, 931
NmeAIII GCCGAG 1 cut(s) 787
NmuCI GTSAC 2 cut(s) 599, 896
PagI TCATGA 2 cut(s) 129, 310
PdmI GAANNNNTTC 1 cut(s) 142
PfeI GAWTC 4 cut(s) 123, 314, 501, 784
PflMI CCANNNNNTGG 1 cut(s) 595
PkrI GCNGC 8 cut(s) 22, 25, 28, 31, 34, 684, 696, 760
PleI GAGTC 1 cut(s) 772
PmaCI CACGTG 1 cut(s) 218
PmlI CACGTG 1 cut(s) 218
PpsI GAGTC 1 cut(s) 772
Ppu21I YACGTR 2 cut(s) 218, 485
PpuMI RGGWCCY 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 366
PspCI CACGTG 1 cut(s) 218
PspN4I GGNNCC 5 cut(s) 367, 368, 391, 465, 931
PspPI GGNCC 2 cut(s) 366, 463
PspPPI RGGWCCY 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 4 cut(s) 574, 642, 955, 1002
RsaNI GTAC 4 cut(s) 573, 641, 954, 1001
RseI CAYNNNNRTG 1 cut(s) 915
SaqAI TTAA 3 cut(s) 41, 68, 882
SatI GCNGC 8 cut(s) 21, 24, 27, 30, 33, 683, 695, 759
Sau3AI GATC 2 cut(s) 76, 439
Sau96I GGNCC 2 cut(s) 366, 463
ScaI AGTACT 1 cut(s) 642
SchI GAGTC 1 cut(s) 773
ScrFI CCNGG 1 cut(s) 799
SfaNI GCATC 2 cut(s) 610, 649
SgrAI CRCCGGYG 1 cut(s) 383
SinI GGWCC 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
Sse9I AATT 8 cut(s) 51, 99, 239, 372, 426, 445, 470, 1017
SsiI CCGC 3 cut(s) 211, 354, 695
SspI AATATT 1 cut(s) 939
SspMI CTAG 4 cut(s) 288, 552, 689, 860
StyD4I CCNGG 1 cut(s) 797
TaaI ACNGT 7 cut(s) 110, 326, 542, 572, 577, 917, 953
TaiI ACGT 4 cut(s) 156, 220, 487, 819
TaqI TCGA 2 cut(s) 499, 948
TasI AATT 8 cut(s) 51, 99, 239, 372, 426, 445, 470, 1017
TatI WGTACW 3 cut(s) 640, 953, 1000
TauI GCSGC 1 cut(s) 697
TfiI GAWTC 4 cut(s) 123, 314, 501, 784
Tru1I TTAA 3 cut(s) 41, 68, 882
Tru9I TTAA 3 cut(s) 41, 68, 882
TscAI CASTG 2 cut(s) 415, 907
TseFI GTSAC 2 cut(s) 599, 896
TseI GCWGC 7 cut(s) 20, 23, 26, 29, 32, 682, 758
Tsp45I GTSAC 2 cut(s) 599, 896
TspDTI ATGAA 1 cut(s) 115
TspRI CASTG 2 cut(s) 415, 907
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 1 cut(s) 366
XapI RAATTY 1 cut(s) 470
XbaI TCTAGA 1 cut(s) 688
XmnI GAANNNNTTC 1 cut(s) 142
XspI CTAG 4 cut(s) 288, 552, 689, 860
ZraI GACGTC 1 cut(s) 154
ZrmI AGTACT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.