Rroxscaffold_5G00333440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014708
Physical Location & Seq
Reverse (-)
857193 .. 858505
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00333440.1

Sequence Viewer

Length: 1200 bp
ATGGAGACGACGAAGTCCCTACACTTGTGTTTCTGTGAGCATATCGCAGCAACAGATGACTCGACTCGCGACGTTGTTCGCTCCATCAAATTGAGCGATTTGTTATCACCACCAACAGCAGCAGCAGAAGCAGCTACTTTTGAGCACGAAACATATCATGATTCTGAAACCCGTGGTTCTGACCCCTTTCCGTGGGGTTGCGGTCGCTTCGGGTCCCAAATTCTCTTCGCCGGCGGCAACCAAGGCTCACGCGACTCCATTTACGGCAAGTCGGTTAAAGGAGAAATTGTTGCCTTTGATCCTAATTCGTGTCCGAAAGCGAAATTCAAGTCCCCTAAATTCACACGACCCCGTAAACATCATTATCAATTCCCAATTGCGCTGTTGGAGGTGGAGGGCAGACTCTATTGTCTTGACACTAGGAGCCAGCAATATTATAGTACTGCTCCCAATTTCATTCTCAGACCGCACTATCACCACTATACCAATGCTCCTCCTGCTTTTGCAGTATTCGACCCCGAAGGTGATGGAGAGTTTGAGGCCAAATGGTTGCCTCTGGAGTCGCCTCCTCCGGCTATACTCTACGATGGGAAATCCCACAAGCTGAACGAGTTTATGTATTGTGCTGTTGGTTCCAAAATCTTAGTCTGGAATCAAGATAAGGCCGGGGTTTACCGCTTTGACGTGAGCCGACCCGAGACAGGATGGATAGAGTTGCACTCTCCTCTAGCAACCACACTTCCCTTTGTTAAGGGTTGTTGGGGTGACACTGCAAAGCTCGCCGATCATCATGGTGGTGGAGGAACCCTAATATTTACCTTTGACTGTACAAGTGGATGTAAACTGCCGGAAATCCCTCCTGAATTTCGCAATGATCCATGCGAGAGGGCAGTTGGTCCAACCGAGTGTTGTCTTGTGCATCTAGGAGATCACAAAGTGTGCCTTATTCTTCACAAATATTTCTACGCATTGTACTGTGACACTAATGATTTGGAGGAAGGCTACGAACTATTTGATCATACCGGCAGAAACAAGGGGATTCTTCATCTCATGACGTTTGAATATCAAGTCACTCCCGACTTGGACATCGAGTACCGACTTCTCACTACTCGCAACTTTGAATACTATACCAAGCTGCCTAACAATGAATATGCGACGTATACCAATGGAGCCTGTCTGACTAAAGCCTTTGTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

399

Amino Acids

45.05

Weight (kDa)

5.83

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 26 - 316 1.8e-09 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 13, 408
AccI GTMKAC 1 cut(s) 1160
AccII CGCG 2 cut(s) 69, 252
AciI CCGC 4 cut(s) 201, 234, 467, 676
AclWI GGATC 2 cut(s) 293, 869
AcsI RAATTY 4 cut(s) 219, 323, 338, 863
AdeI CACNNNGTG 1 cut(s) 937
AfaI GTAC 4 cut(s) 442, 829, 974, 1094
AfiI CCNNNNNNNGG 2 cut(s) 192, 701
AgsI TTSAA 3 cut(s) 328, 1061, 1121
AjiI CACGTC 1 cut(s) 685
AluBI AGCT 4 cut(s) 134, 604, 778, 1135
AluI AGCT 4 cut(s) 134, 604, 778, 1135
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 1 cut(s) 692
AlwI GGATC 2 cut(s) 293, 869
Ama87I CYCGRG 1 cut(s) 695
AoxI GGCC 2 cut(s) 540, 663
ApeKI GCWGC 5 cut(s) 47, 119, 122, 131, 1135
ApoI RAATTY 4 cut(s) 219, 323, 338, 863
ArsI GACNNNNNNTTYG 4 cut(s) 314, 346, 630, 662
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 2 cut(s) 213, 896
AsuC2I CCSGG 1 cut(s) 667
AsuHPI GGTGA 4 cut(s) 99, 467, 536, 776
AvaI CYCGRG 1 cut(s) 695
AvaII GGWCC 2 cut(s) 213, 896
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 5 cut(s) 59, 131, 134, 143, 1122
BccI CCATC 4 cut(s) 92, 521, 581, 699
BceAI ACGGC 1 cut(s) 280
BclI TGATCA 1 cut(s) 1015
BcnI CCSGG 1 cut(s) 667
BcoDI GTCTC 1 cut(s) 692
BfaI CTAG 3 cut(s) 420, 728, 923
BisI GCNGC 6 cut(s) 48, 120, 123, 132, 235, 1136
BlsI GCNGC 6 cut(s) 49, 121, 124, 133, 236, 1137
BmcAI AGTACT 1 cut(s) 442
Bme1390I CCNGG 1 cut(s) 667
Bme18I GGWCC 2 cut(s) 213, 896
BmeT110I CYCGRG 1 cut(s) 695
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 2 cut(s) 213, 896
BmiI GGNNCC 6 cut(s) 214, 215, 425, 634, 805, 1171
BmrFI CCNGG 1 cut(s) 667
BmsI GCATC 1 cut(s) 928
BplI GAGNNNNNCTC 2 cut(s) 704, 736
BpmI CTGGAG 1 cut(s) 578
BpuMI CCSGG 1 cut(s) 667
BsaJI CCNNGG 4 cut(s) 172, 191, 241, 666
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 475, 505
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 701
Bse118I RCCGGY 2 cut(s) 230, 1022
Bse3DI GCAATG 1 cut(s) 877
BseDI CCNNGG 4 cut(s) 172, 191, 241, 666
BseGI GGATG 2 cut(s) 710, 842
BseLI CCNNNNNNNGG 2 cut(s) 192, 701
BseMI GCAATG 1 cut(s) 877
BseMII CTCAG 1 cut(s) 475
BseRI GAGGAG 3 cut(s) 483, 558, 714
BseXI GCAGC 5 cut(s) 59, 131, 134, 143, 1122
Bsh1236I CGCG 2 cut(s) 69, 252
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 2 cut(s) 542, 665
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 5 cut(s) 231, 572, 666, 848, 1023
BslFI GGGAC 2 cut(s) 199, 316
BslI CCNNNNNNNGG 2 cut(s) 192, 701
BsmAI GTCTC 1 cut(s) 692
BsmFI GGGAC 2 cut(s) 199, 316
BsnI GGCC 2 cut(s) 542, 665
BsoBI CYCGRG 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 147
Bsp1407I TGTACA 1 cut(s) 827
Bsp143I GATC 5 cut(s) 298, 784, 874, 928, 1015
Bsp68I TCGCGA 1 cut(s) 69
BspACI CCGC 4 cut(s) 201, 234, 467, 676
BspANI GGCC 2 cut(s) 542, 665
BspCNI CTCAG 1 cut(s) 474
BspFNI CGCG 2 cut(s) 69, 252
BspHI TCATGA 2 cut(s) 157, 1050
BspLI GGNNCC 6 cut(s) 214, 215, 425, 634, 805, 1171
BspPI GGATC 2 cut(s) 293, 869
BsrDI GCAATG 1 cut(s) 877
BsrFI RCCGGY 2 cut(s) 230, 1022
BsrGI TGTACA 1 cut(s) 827
BssAI RCCGGY 2 cut(s) 230, 1022
BssECI CCNNGG 4 cut(s) 172, 191, 241, 666
BssMI GATC 5 cut(s) 298, 784, 874, 928, 1015
BssNAI GTATAC 1 cut(s) 1161
BssT1I CCWWGG 1 cut(s) 241
Bst1107I GTATAC 1 cut(s) 1161
Bst4CI ACNGT 2 cut(s) 827, 977
Bst6I CTCTTC 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 827
BstC8I GCNNGC 3 cut(s) 232, 428, 780
BstDEI CTNAG 2 cut(s) 461, 643
BstDSI CCRYGG 2 cut(s) 172, 191
BstF5I GGATG 2 cut(s) 710, 842
BstFNI CGCG 2 cut(s) 69, 252
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 5 cut(s) 301, 787, 877, 931, 1018
BstMAI GTCTC 1 cut(s) 692
BstMBI GATC 5 cut(s) 298, 784, 874, 928, 1015
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 5 cut(s) 128, 131, 243, 497, 779
BstSCI CCNGG 1 cut(s) 665
BstUI CGCG 2 cut(s) 69, 252
BstV1I GCAGC 5 cut(s) 59, 131, 134, 143, 1122
BstZ17I GTATAC 1 cut(s) 1161
BsuRI GGCC 2 cut(s) 542, 665
BtgI CCRYGG 2 cut(s) 172, 191
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 2 cut(s) 710, 842
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 768
BtuMI TCGCGA 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 232, 428, 780
CciI TCATGA 2 cut(s) 157, 1050
CfoI GCGC 1 cut(s) 382
Cfr10I RCCGGY 2 cut(s) 230, 1022
Cfr13I GGNCC 2 cut(s) 213, 896
Csp6I GTAC 4 cut(s) 441, 828, 973, 1093
CviAII CATG 4 cut(s) 158, 791, 879, 1051
CviQI GTAC 4 cut(s) 441, 828, 973, 1093
DdeI CTNAG 2 cut(s) 461, 643
DpnI GATC 5 cut(s) 300, 786, 876, 930, 1017
DpnII GATC 5 cut(s) 298, 784, 874, 928, 1015
DraIII CACNNNGTG 1 cut(s) 937
DrdI GACNNNNNNGTC 2 cut(s) 13, 408
DseDI GACNNNNNNGTC 2 cut(s) 13, 408
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 213, 896
Eco88I CYCGRG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 4 cut(s) 161, 794, 882, 1054
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 2 cut(s) 199, 316
FatI CATG 4 cut(s) 157, 790, 878, 1050
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 1 cut(s) 1160
Fnu4HI GCNGC 6 cut(s) 48, 120, 123, 132, 235, 1136
FokI GGATG 2 cut(s) 717, 849
Fsp4HI GCNGC 6 cut(s) 48, 120, 123, 132, 235, 1136
FspBI CTAG 3 cut(s) 420, 728, 923
GlaI GCGC 1 cut(s) 381
GluI GCNGC 6 cut(s) 48, 120, 123, 132, 235, 1136
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 2 cut(s) 542, 665
HapII CCGG 5 cut(s) 231, 572, 666, 848, 1023
HhaI GCGC 1 cut(s) 382
Hin1II CATG 4 cut(s) 161, 794, 882, 1054
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HinfI GANTC 8 cut(s) 59, 64, 161, 254, 402, 560, 652, 1039
HpaII CCGG 5 cut(s) 231, 572, 666, 848, 1023
HphI GGTGA 4 cut(s) 99, 467, 536, 776
Hpy166II GTNNAC 4 cut(s) 356, 673, 842, 1161
Hpy188I TCNGA 5 cut(s) 166, 181, 315, 464, 1179
Hpy188III TCNNGA 9 cut(s) 68, 158, 413, 557, 649, 656, 860, 1051, 1076
Hpy8I GTNNAC 4 cut(s) 356, 673, 842, 1161
Hpy99I CGWCG 3 cut(s) 13, 74, 1159
HpyAV CCTTC 2 cut(s) 515, 992
HpyCH4III ACNGT 2 cut(s) 827, 977
HpyCH4IV ACGT 4 cut(s) 72, 684, 1055, 1157
HpyCH4V TGCA 4 cut(s) 506, 718, 773, 919
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 131, 243, 497, 779
HpyF3I CTNAG 2 cut(s) 461, 643
HpySE526I ACGT 4 cut(s) 72, 684, 1055, 1157
Hsp92II CATG 4 cut(s) 161, 794, 882, 1054
HspAI GCGC 1 cut(s) 380
KflI GGGWCCC 1 cut(s) 213
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 1015
Kzo9I GATC 5 cut(s) 298, 784, 874, 928, 1015
LmnI GCTCC 5 cut(s) 86, 423, 451, 496, 1169
Lsp1109I GCAGC 5 cut(s) 59, 131, 134, 143, 1122
LweI GCATC 1 cut(s) 928
MaeI CTAG 3 cut(s) 420, 728, 923
MaeII ACGT 4 cut(s) 72, 684, 1055, 1157
MaeIII GTNAC 3 cut(s) 764, 977, 1069
MalI GATC 5 cut(s) 300, 786, 876, 930, 1017
MboI GATC 5 cut(s) 298, 784, 874, 928, 1015
MboII GAAGA 3 cut(s) 217, 941, 1034
MfeI CAATTG 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 147
MlyI GAGTC 5 cut(s) 53, 58, 248, 396, 569
MmeI TCCRAC 2 cut(s) 366, 923
MreI CGCCGGCG 1 cut(s) 230
MroNI GCCGGC 1 cut(s) 230
MseI TTAA 2 cut(s) 276, 750
MslI CAYNNNNRTG 2 cut(s) 792, 795
MspI CCGG 5 cut(s) 231, 572, 666, 848, 1023
MspR9I CCNGG 1 cut(s) 667
MunI CAATTG 1 cut(s) 375
MvnI CGCG 2 cut(s) 69, 252
MwoI GCNNNNNNNGC 5 cut(s) 128, 131, 243, 497, 779
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 1 cut(s) 667
NdeII GATC 5 cut(s) 298, 784, 874, 928, 1015
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 4 cut(s) 161, 794, 882, 1054
NlaIV GGNNCC 6 cut(s) 214, 215, 425, 634, 805, 1171
NmuCI GTSAC 3 cut(s) 764, 977, 1069
NruI TCGCGA 1 cut(s) 69
PagI TCATGA 2 cut(s) 157, 1050
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 3 cut(s) 161, 652, 1039
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 6 cut(s) 49, 121, 124, 133, 236, 1137
PleI GAGTC 5 cut(s) 53, 58, 248, 396, 568
PpsI GAGTC 5 cut(s) 53, 58, 248, 396, 568
PpuMI RGGWCCY 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 213
PspN4I GGNNCC 6 cut(s) 214, 215, 425, 634, 805, 1171
PspPI GGNCC 2 cut(s) 213, 896
PspPPI RGGWCCY 1 cut(s) 213
PsyI GACNNNGTC 1 cut(s) 13
RruI TCGCGA 1 cut(s) 69
RsaI GTAC 4 cut(s) 442, 829, 974, 1094
RsaNI GTAC 4 cut(s) 441, 828, 973, 1093
RseI CAYNNNNRTG 2 cut(s) 792, 795
SaqAI TTAA 2 cut(s) 276, 750
SatI GCNGC 6 cut(s) 48, 120, 123, 132, 235, 1136
Sau3AI GATC 5 cut(s) 298, 784, 874, 928, 1015
Sau96I GGNCC 2 cut(s) 213, 896
ScaI AGTACT 1 cut(s) 442
SchI GAGTC 5 cut(s) 53, 58, 248, 396, 569
ScrFI CCNGG 1 cut(s) 667
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 1 cut(s) 928
SgrAI CRCCGGYG 1 cut(s) 230
SinI GGWCC 2 cut(s) 213, 896
SmiMI CAYNNNNRTG 2 cut(s) 792, 795
SsiI CCGC 4 cut(s) 201, 234, 467, 676
SspI AATATT 3 cut(s) 434, 813, 959
SspMI CTAG 3 cut(s) 420, 728, 923
StyD4I CCNGG 1 cut(s) 665
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 827, 977
TaiI ACGT 4 cut(s) 75, 687, 1058, 1160
TaqI TCGA 3 cut(s) 62, 513, 1089
TatI WGTACW 3 cut(s) 440, 827, 972
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 161, 652, 1039
Tru1I TTAA 2 cut(s) 276, 750
Tru9I TTAA 2 cut(s) 276, 750
TscAI CASTG 1 cut(s) 775
TseFI GTSAC 3 cut(s) 764, 977, 1069
TseI GCWGC 5 cut(s) 47, 119, 122, 131, 1135
Tsp45I GTSAC 3 cut(s) 764, 977, 1069
TspDTI ATGAA 3 cut(s) 445, 1034, 1161
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 775
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 213, 896
XapI RAATTY 4 cut(s) 219, 323, 338, 863
XmiI GTMKAC 1 cut(s) 1160
XspI CTAG 3 cut(s) 420, 728, 923
ZrmI AGTACT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.