Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0002851

Physical Location & Seq

Genomic Coordinates Forward (+)

2528472 .. 2536316

Gene Structure 7845 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g02820.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGCTTGCTGGAGTGAAATTCATACCTCGAGATCAGATTGATAAAGATGAGAATTTAAAGGCTTCGGCAAAACAGAAACAGAAGAAGGGCTCCGGTAGTAAGGAGGGTAAGCATAGGAGGGAAAGGAGTTCGGGATACAGTAGTTCCGATGGCGAAGAACTTGAGAGAATAAAGAAGGGATCTAAAAAAAAGTGGTACTCATCGGATGAATACTTATCATCATCATATAGTTCAGAAAATGAAAGTGAGGGAAGCTCTGACCGAGATAGAAAGAAGCGTCACACTAAGAAAAAGAAGAGATCGAGTAGCAGTAGAACAGAATATGGTTCAGAAGATCTTTCATCTGCTTCTGATAGTTATTCTGATAGGAAAGACAAGCATAAGAAGAGAAGCCGAAAAGAGGGAAAGAAAAAGAGTGGAGATAGAAGTCAAAGAGAGGGAGGTAGAGGAACTACTACGGTGAAAGATGGGGAAATTGTGAGGAAGAAAATAGGGTTGGAGTGGATGCTTAGACCTGAAGGAAAAGAGAGAACACCTGCTATTACTGTTGAGGACCAGCCCAAAGAAATTCCAGTTGAAGAGATAAAGAAGGTTAATCCTAAGGAATTAAATCCATATTATAAGGATGATGGAAGCGGTTATCCAGAAGATAATGATGAACCTAAATCTGGTGTGAAATTACCTTCCTCTGTTGTTGGGGATGGAGGTGCAAGTTGGAGACTTAAAGCATGGAAGCGGGCACAAGAGCAAGCAGCTCGAGAAGGACAAGGGATCCACGAGGTTGTTGGGGAGCGTCATGGTTCTCTCGGTGAACTAGTGGCCTCTGTGCATTCAAATAAAGCAGCCCATTCTCGTGCTCATTTACATGCAATACAAAGTAGAAAAAAAGGGGTGAGTGAGGAACAGCAGCCAGGTTCCAACAATGACATGGATAAAAAGGACACTAGTCGAAAGGATGTATCTATCCGACATCCTGAAATGAGGGAGCCTAATGTTCAAGGTTCTTTTTCCTGGAGAAAACAAAAGGGTCAAAAGAGTCGAGATATCTCTACCAAGGACTCTGCTCTTCTAGCAGATGCAGTGTCTAGCATAAACAGGTTTTCCAACGATGGAAGCTTCCTGAGTGAACTTATTCGTAAGCAGACTAATGATTCCAGAAATCCTGTTAACGAAAATGTACTAGACACGAGAAAACCCAGCGAAGCCAGTGCAGCTGCCTCTGTGGGTGATCTGAGTGCGAACCAGTTGGCAGCTAAGGCTTATCAGCTTAAGATGAAAGGGAAGCTTGAGGAAGCTGAGGAACTTCTGCAAGAAGTAGAAAATCTTAAGGGAAAGCAGGGTGCTGGAGAAAATCCGATGAGACCACAATTTGAAAGAGGTACAAACAGATATGCTATGCAGGATATGTCGCTCCGTCAAAAGAAAAAGGATGATGATGGTGACATGCATCTAGCCCAAAAGATAATGCAGAACAAAAAGTATAGTATATCTGGTCGGGCAGATGATGAATATGACTTTGATGATGGTCCAAGCAGAAAGTCTAAAAAGAAGCAGGGAAGTGATGATCGCAAGGTCACCCAGAATAATACATTTGCAAATCGTTTCTCCATTCAGCAAGAACGCTGCCTTTTCTGTTTTGAAAATCCGAAGAGACCAGCGCATCTGGTTGTGGCAATTGGAAATTATTCATATTTGATGTTGCCACAGCAGAAGCCTGTAGTGGATGGTCATTGCTACGTTTTGCCGATGCAGCATGTACCAGCCACAAGAACTCTTGATGATCATGTGTGGGAAGAAATTCGAAATTTCAAGAAATGCCTTATTATGATGTTTGCAAAGCAAGAGAAGGATGTTGTGTTCCTTGAAACAGTGATGGGGTTGGCTCAACAGCGCCGCCACTGTATAATCGAGTGCATTCCTTTGCCCCAGGATATAGCTAAAGACGCTCCCTTATACTTTAAGAAGGCAATTGATGAAGCTGAAGATGAGTGGAGCCAACACAATGCCAAAAAGCTTATTGATACAAGTGTAAAGGGATTGCGAGGTTCAGTTCCAGCTAACTTCCCATATTTCCATGTGGAGTTCGGTTTAAAGAAGGGGTTCGTCCACGTGATTGATGATGAAACACAGTTCAATAGCAGCCTTGGCCTGAATGTTATAAGAGGCATGCTAGAGTTGCCTGCGGAAGACATGCATCGTCGTGGAAGGTATGAGCCAGTGGAAGCGCAAAAGCAAGCCGTTAAGAGGTTTGATCAAGAGTGGGCACCGTTTGACTGGACAAAACAGCTACATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CwfJ_C_1 CwfJ_C_2

Protein Analysis

765

Amino Acids

86.73

Weight (kDa)

9.28

Isoelectric Point (pI)

44.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CwfJ_C_1	PF04677	520 - 640	2.8e-42	Protein similar to CwfJ C-terminal
CwfJ_C_2	PF04676	649 - 746	4.2e-27	Protein similar to CwfJ C-terminus 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56290
fragaria_vesca	FvH4_4g02820 FvH4_4g02820 FvH4_4g02820 FvH4_4g02820
malus_domestica	MD13G1208100.v1.1 MD16G1209600.v1.1
prunus_persica	Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1
pyrus_communis	pycom13g18020 pycom16g17690
rosa_chinensis	RchiOBHm_Chr4g0390791 RchiOBHm_Chr5g0000461
rosa_laevigata	RLG00000009911 RLG00000030869
rosa_multiflora	Rmu_sc0000171.1_g000026 Rmu_sc0001380.1_g000027 Rmu_sc0003949.1_g000006
rosa_roxburghii	Rroxscaffold_1G00075700 Rroxscaffold_5G00336660 Rroxscaffold_5G00336680 Rroxscaffold_5G00336690
rosa_rugosa	Rorug03G0331500 Rorug03G0331500 Rorug04G0383800
rosa_samantha	Rh4AG033200 Rh4BG027000 Rh4CG036800 Rh4DG030000 Rh5AG003800 Rh5BG005000 Rh5CG004100 Rh5DG004000
rosa_wichuraiana	Rw5G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	621, 2159
AarI	CACCTGC	1 cut(s)	544
Acc36I	ACCTGC	1 cut(s)	544
AccB1I	GGYRCC	1 cut(s)	2263
AciI	CCGC	4 cut(s)	636, 736, 1894, 2183
AclWI	GGATC	3 cut(s)	187, 766, 779
AcsI	RAATTY	5 cut(s)	17, 52, 567, 1797, 1804
AcuI	CTGAAG	2 cut(s)	537, 2001
AcvI	CACGTG	1 cut(s)	2110
AfaI	GTAC	4 cut(s)	197, 1179, 1381, 1758
AfiI	CCNNNNNNNGG	3 cut(s)	400, 668, 2244
AflII	CTTAAG	2 cut(s)	1268, 1325
AgsI	TTSAA	8 cut(s)	578, 834, 998, 1373, 1640, 1810, 1865, 2134
AhlI	ACTAGT	2 cut(s)	814, 944
AjnI	CCWGG	3 cut(s)	910, 1010, 1926
AjuI	GAANNNNNNNTTGG	2 cut(s)	479, 511
AloI	GAACNNNNNNTCC	2 cut(s)	1841, 1873
Alw21I	GWGCWC	1 cut(s)	859
Alw26I	GTCTC	3 cut(s)	712, 1354, 1645
AlwI	GGATC	3 cut(s)	187, 766, 779
Ama87I	CYCGRG	2 cut(s)	27, 756
AoxI	GGCC	2 cut(s)	819, 2146
ApeKI	GCWGC	9 cut(s)	752, 842, 907, 1211, 1214, 1250, 1623, 1750, 2139
ApoI	RAATTY	5 cut(s)	17, 52, 567, 1797, 1804
Asp700I	GAANNNNTTC	4 cut(s)	1131, 1684, 1797, 2099
AspLEI	GCGC	3 cut(s)	1660, 1893, 2227
AspS9I	GGNCC	2 cut(s)	553, 1526
AsuHPI	GGTGA	6 cut(s)	472, 821, 904, 1238, 1451, 1567
AsuII	TTCGAA	1 cut(s)	1801
AvaI	CYCGRG	2 cut(s)	27, 756
AvaII	GGWCC	2 cut(s)	553, 1526
AxyI	CCTNAGG	1 cut(s)	600
BaeGI	GKGCMC	2 cut(s)	742, 2266
BaeI	ACNNNNGTAYC	4 cut(s)	127, 160, 2013, 2046
BamHI	GGATCC	1 cut(s)	771
BanI	GGYRCC	1 cut(s)	2263
BanII	GRGCYC	1 cut(s)	92
BauI	CACGAG	3 cut(s)	776, 852, 1186
BbrPI	CACGTG	1 cut(s)	2110
BbsI	GAAGAC	1 cut(s)	2193
Bbv12I	GWGCWC	1 cut(s)	859
BbvCI	CCTCAGC	1 cut(s)	1296
BbvI	GCAGC	9 cut(s)	764, 854, 919, 1201, 1223, 1262, 1610, 1762, 2151
BccI	CCATC	9 cut(s)	143, 461, 623, 695, 1103, 1430, 1517, 1718, 1867
BceAI	ACGGC	1 cut(s)	2222
BcgI	CGANNNNNNTGC	4 cut(s)	737, 771, 1190, 1224
BciT130I	CCWGG	3 cut(s)	912, 1012, 1928
BciVI	GTATCC	1 cut(s)	128
BclI	TGATCA	2 cut(s)	1780, 2251
BcoDI	GTCTC	3 cut(s)	712, 1354, 1645
BcuI	ACTAGT	2 cut(s)	814, 944
BfaI	CTAG	7 cut(s)	815, 945, 1070, 1086, 1181, 1451, 2171
BfmI	CTRYAG	1 cut(s)	1716
BfoI	RGCGCY	1 cut(s)	1894
BfrI	CTTAAG	2 cut(s)	1268, 1325
BfuAI	ACCTGC	1 cut(s)	544
BfuI	GTATCC	1 cut(s)	128
BglII	AGATCT	1 cut(s)	334
Bme1390I	CCNGG	3 cut(s)	912, 1012, 1928
Bme18I	GGWCC	2 cut(s)	553, 1526
BmeT110I	CYCGRG	2 cut(s)	27, 756
BmgT120I	GGNCC	2 cut(s)	553, 1526
BmiI	GGNNCC	6 cut(s)	91, 773, 916, 987, 1994, 2265
BmrFI	CCNGG	3 cut(s)	912, 1012, 1928
BmsI	GCATC	6 cut(s)	495, 1066, 1456, 1669, 1737, 2203
BoxI	GACNNNNGTC	1 cut(s)	945
BpiI	GAAGAC	1 cut(s)	2193
BplI	GAGNNNNNCTC	2 cut(s)	239, 271
BpmI	CTGGAG	3 cut(s)	30, 1033, 1365
Bpu10I	CCTNAGC	2 cut(s)	1254, 1296
Bpu14I	TTCGAA	1 cut(s)	1801
BpuEI	CTTGAG	2 cut(s)	182, 1307
BsaAI	YACGTR	1 cut(s)	2110
BsaI	GGTCTC	2 cut(s)	1354, 1645
BsaJI	CCNNGG	3 cut(s)	1053, 1926, 2143
BsaWI	WCCGGW	1 cut(s)	92
Bsc4I	CCNNNNNNNGG	3 cut(s)	400, 668, 2244
Bse1I	ACTGG	5 cut(s)	572, 1206, 1243, 2216, 2279
Bse21I	CCTNAGG	1 cut(s)	600
Bse3DI	GCAATG	1 cut(s)	1729
BseBI	CCWGG	3 cut(s)	912, 1012, 1928
BseDI	CCNNGG	3 cut(s)	1053, 1926, 2143
BseGI	GGATG	9 cut(s)	211, 510, 631, 706, 961, 970, 1435, 1729, 1855
BseLI	CCNNNNNNNGG	3 cut(s)	400, 668, 2244
BseMI	GCAATG	1 cut(s)	1729
BseMII	CTCAG	3 cut(s)	1112, 1223, 1287
BseNI	ACTGG	5 cut(s)	572, 1206, 1243, 2216, 2279
BseSI	GKGCMC	2 cut(s)	742, 2266
BseXI	GCAGC	9 cut(s)	764, 854, 919, 1201, 1223, 1262, 1610, 1762, 2151
BseYI	CCCAGC	1 cut(s)	1196
BsgI	GTGCAG	1 cut(s)	1230
BshFI	GGCC	2 cut(s)	821, 2148
BshNI	GGYRCC	1 cut(s)	2263
BsiHKAI	GWGCWC	1 cut(s)	859
BsiHKCI	CYCGRG	2 cut(s)	27, 756
BsiSI	CCGG	1 cut(s)	93
BslI	CCNNNNNNNGG	3 cut(s)	400, 668, 2244
BsmAI	GTCTC	3 cut(s)	712, 1354, 1645
BsmI	GAATGC	2 cut(s)	829, 1914
BsnI	GGCC	2 cut(s)	821, 2148
Bso31I	GGTCTC	2 cut(s)	1354, 1645
BsoBI	CYCGRG	2 cut(s)	27, 756
Bsp119I	TTCGAA	1 cut(s)	1801
Bsp1286I	GDGCHC	4 cut(s)	92, 742, 859, 2266
Bsp143I	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
BspACI	CCGC	4 cut(s)	636, 736, 1894, 2183
BspANI	GGCC	2 cut(s)	821, 2148
BspCNI	CTCAG	3 cut(s)	1113, 1224, 1288
BspLI	GGNNCC	6 cut(s)	91, 773, 916, 987, 1994, 2265
BspMI	ACCTGC	1 cut(s)	544
BspPI	GGATC	3 cut(s)	187, 766, 779
BspQI	GCTCTTC	1 cut(s)	1071
BspT104I	TTCGAA	1 cut(s)	1801
BspT107I	GGYRCC	1 cut(s)	2263
BspTI	CTTAAG	2 cut(s)	1268, 1325
BspTNI	GGTCTC	2 cut(s)	1354, 1645
BsrDI	GCAATG	1 cut(s)	1729
BsrI	ACTGG	5 cut(s)	572, 1206, 1243, 2216, 2279
BssECI	CCNNGG	3 cut(s)	1053, 1926, 2143
BssMI	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
BssSI	CACGAG	3 cut(s)	776, 852, 1186
BssT1I	CCWWGG	2 cut(s)	1053, 2143
Bst2BI	CACGAG	3 cut(s)	776, 852, 1186
Bst2UI	CCWGG	3 cut(s)	912, 1012, 1928
Bst4CI	ACNGT	7 cut(s)	140, 460, 547, 1870, 1901, 2130, 2268
Bst6I	CTCTTC	5 cut(s)	290, 380, 573, 1071, 1643
BstAFI	CTTAAG	2 cut(s)	1268, 1325
BstBAI	YACGTR	1 cut(s)	2110
BstBI	TTCGAA	1 cut(s)	1801
BstC8I	GCNNGC	6 cut(s)	6, 738, 750, 2168, 2181, 2235
BstDEI	CTNAG	7 cut(s)	285, 509, 600, 1121, 1232, 1254, 1296
BstEII	GGTNACC	1 cut(s)	1573
BstF5I	GGATG	9 cut(s)	211, 510, 631, 706, 961, 970, 1435, 1729, 1855
BstH2I	RGCGCY	1 cut(s)	1894
BstHHI	GCGC	3 cut(s)	1660, 1893, 2227
BstKTI	GATC	9 cut(s)	34, 182, 302, 337, 774, 1231, 1567, 1783, 2254
BstMAI	GTCTC	3 cut(s)	712, 1354, 1645
BstMBI	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
BstMWI	GCNNNNNNNGC	7 cut(s)	1070, 1211, 1256, 1750, 1943, 2145, 2176
BstNI	CCWGG	3 cut(s)	912, 1012, 1928
BstNSI	RCATGY	5 cut(s)	869, 1447, 1757, 2170, 2194
BstPAI	GACNNNNGTC	1 cut(s)	945
BstPI	GGTNACC	1 cut(s)	1573
BstSCI	CCNGG	3 cut(s)	910, 1010, 1926
BstSFI	CTRYAG	1 cut(s)	1716
BstSLI	GKGCMC	2 cut(s)	742, 2266
BstV1I	GCAGC	9 cut(s)	764, 854, 919, 1201, 1223, 1262, 1610, 1762, 2151
BstV2I	GAAGAC	1 cut(s)	2193
BstX2I	RGATCY	3 cut(s)	179, 334, 771
BstYI	RGATCY	3 cut(s)	179, 334, 771
Bsu36I	CCTNAGG	1 cut(s)	600
BsuI	GTATCC	1 cut(s)	128
BsuRI	GGCC	2 cut(s)	821, 2148
BtsCI	GGATG	9 cut(s)	211, 510, 631, 706, 961, 970, 1435, 1729, 1855
BtsI	GCAGTG	1 cut(s)	1086
BtsIMutI	CAGTG	5 cut(s)	1086, 1213, 1875, 1897, 2223
BveI	ACCTGC	1 cut(s)	544
Cac8I	GCNNGC	6 cut(s)	6, 738, 750, 2168, 2181, 2235
CfoI	GCGC	3 cut(s)	1660, 1893, 2227
Cfr13I	GGNCC	2 cut(s)	553, 1526
CseI	GACGC	3 cut(s)	266, 782, 1952
Csp6I	GTAC	4 cut(s)	196, 1178, 1380, 1757
CviQI	GTAC	4 cut(s)	196, 1178, 1380, 1757
DdeI	CTNAG	7 cut(s)	285, 509, 600, 1121, 1232, 1254, 1296
DpnI	GATC	9 cut(s)	33, 181, 301, 336, 773, 1230, 1566, 1782, 2253
DpnII	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
DraI	TTTAAA	2 cut(s)	57, 2091
Eam1104I	CTCTTC	5 cut(s)	290, 380, 573, 1071, 1643
EarI	CTCTTC	5 cut(s)	290, 380, 573, 1071, 1643
Eco130I	CCWWGG	2 cut(s)	1053, 2143
Eco24I	GRGCYC	1 cut(s)	92
Eco31I	GGTCTC	2 cut(s)	1354, 1645
Eco32I	GATATC	1 cut(s)	1045
Eco47I	GGWCC	2 cut(s)	553, 1526
Eco57I	CTGAAG	2 cut(s)	537, 2001
Eco72I	CACGTG	1 cut(s)	2110
Eco81I	CCTNAGG	1 cut(s)	600
Eco88I	CYCGRG	2 cut(s)	27, 756
Eco91I	GGTNACC	1 cut(s)	1573
EcoO65I	GGTNACC	1 cut(s)	1573
EcoRII	CCWGG	3 cut(s)	910, 1010, 1926
EcoRV	GATATC	1 cut(s)	1045
EcoT14I	CCWWGG	2 cut(s)	1053, 2143
EcoT22I	ATGCAT	2 cut(s)	1449, 2196
EcoT38I	GRGCYC	1 cut(s)	92
ErhI	CCWWGG	2 cut(s)	1053, 2143
FalI	AAGNNNNNCTT	2 cut(s)	1269, 1301
FauI	CCCGC	1 cut(s)	729
FbaI	TGATCA	2 cut(s)	1780, 2251
FokI	GGATG	9 cut(s)	218, 517, 638, 713, 957, 968, 1442, 1736, 1862
FriOI	GRGCYC	1 cut(s)	92
FspBI	CTAG	7 cut(s)	815, 945, 1070, 1086, 1181, 1451, 2171
GlaI	GCGC	3 cut(s)	1659, 1892, 2226
GsaI	CCCAGC	1 cut(s)	1200
GsuI	CTGGAG	3 cut(s)	30, 1033, 1365
HaeII	RGCGCY	1 cut(s)	1894
HaeIII	GGCC	2 cut(s)	821, 2148
HapII	CCGG	1 cut(s)	93
HgaI	GACGC	3 cut(s)	266, 782, 1952
HhaI	GCGC	3 cut(s)	1660, 1893, 2227
Hin6I	GCGC	3 cut(s)	1658, 1891, 2225
HinP1I	GCGC	3 cut(s)	1658, 1891, 2225
HincII	GTYRAC	1 cut(s)	1168
HindII	GTYRAC	1 cut(s)	1168
HindIII	AAGCTT	3 cut(s)	1114, 1283, 2012
HinfI	GANTC	3 cut(s)	1036, 1058, 1151
HpaI	GTTAAC	1 cut(s)	1168
HpaII	CCGG	1 cut(s)	93
HphI	GGTGA	6 cut(s)	472, 821, 904, 1238, 1451, 1567
Hpy166II	GTNNAC	4 cut(s)	812, 1127, 1168, 2107
Hpy8I	GTNNAC	4 cut(s)	812, 1127, 1168, 2107
Hpy99I	CGWCG	1 cut(s)	2202
HpyCH4III	ACNGT	7 cut(s)	140, 460, 547, 1870, 1901, 2130, 2268
HpyCH4IV	ACGT	2 cut(s)	1737, 2109
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1070, 1211, 1256, 1750, 1943, 2145, 2176
HpyF3I	CTNAG	7 cut(s)	285, 509, 600, 1121, 1232, 1254, 1296
HpySE526I	ACGT	2 cut(s)	1737, 2109
HspAI	GCGC	3 cut(s)	1658, 1891, 2225
Ksp22I	TGATCA	2 cut(s)	1780, 2251
KspAI	GTTAAC	1 cut(s)	1168
Kzo9I	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
LguI	GCTCTTC	1 cut(s)	1071
LmnI	GCTCC	6 cut(s)	95, 790, 985, 1416, 1951, 1992
Lsp1109I	GCAGC	9 cut(s)	764, 854, 919, 1201, 1223, 1262, 1610, 1762, 2151
LweI	GCATC	6 cut(s)	495, 1066, 1456, 1669, 1737, 2203
MaeI	CTAG	7 cut(s)	815, 945, 1070, 1086, 1181, 1451, 2171
MaeII	ACGT	2 cut(s)	1737, 2109
MaeIII	GTNAC	3 cut(s)	278, 1439, 1573
MalI	GATC	9 cut(s)	33, 181, 301, 336, 773, 1230, 1566, 1782, 2253
MboI	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
MfeI	CAATTG	2 cut(s)	1674, 1968
MflI	RGATCY	3 cut(s)	179, 334, 771
MhlI	GDGCHC	4 cut(s)	92, 742, 859, 2266
MlyI	GAGTC	2 cut(s)	1045, 1052
MmeI	TCCRAC	5 cut(s)	477, 695, 942, 991, 1128
Mph1103I	ATGCAT	2 cut(s)	1449, 2196
MroXI	GAANNNNTTC	4 cut(s)	1131, 1684, 1797, 2099
MslI	CAYNNNNRTG	3 cut(s)	852, 864, 2199
MspA1I	CMGCKG	1 cut(s)	1214
MspCI	CTTAAG	2 cut(s)	1268, 1325
MspI	CCGG	1 cut(s)	93
MspR9I	CCNGG	3 cut(s)	912, 1012, 1928
MunI	CAATTG	2 cut(s)	1674, 1968
Mva1269I	GAATGC	2 cut(s)	829, 1914
MvaI	CCWGG	3 cut(s)	912, 1012, 1928
MwoI	GCNNNNNNNGC	7 cut(s)	1070, 1211, 1256, 1750, 1943, 2145, 2176
NdeII	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
NlaIV	GGNNCC	6 cut(s)	91, 773, 916, 987, 1994, 2265
NmuCI	GTSAC	3 cut(s)	278, 1439, 1573
NsiI	ATGCAT	2 cut(s)	1449, 2196
NspI	RCATGY	5 cut(s)	869, 1447, 1757, 2170, 2194
NspV	TTCGAA	1 cut(s)	1801
PaeI	GCATGC	1 cut(s)	2170
PaeR7I	CTCGAG	2 cut(s)	27, 756
PaqCI	CACCTGC	1 cut(s)	544
PciSI	GCTCTTC	1 cut(s)	1071
PctI	GAATGC	2 cut(s)	829, 1914
PdmI	GAANNNNTTC	4 cut(s)	1131, 1684, 1797, 2099
PfeI	GAWTC	1 cut(s)	1151
PfoI	TCCNGGA	1 cut(s)	1010
PleI	GAGTC	2 cut(s)	1044, 1052
PmaCI	CACGTG	1 cut(s)	2110
PmlI	CACGTG	1 cut(s)	2110
PpsI	GAGTC	2 cut(s)	1044, 1052
Ppu21I	YACGTR	1 cut(s)	2110
PshAI	GACNNNNGTC	1 cut(s)	945
PsiI	TTATAA	2 cut(s)	621, 2159
Psp6I	CCWGG	3 cut(s)	910, 1010, 1926
PspCI	CACGTG	1 cut(s)	2110
PspEI	GGTNACC	1 cut(s)	1573
PspFI	CCCAGC	1 cut(s)	1196
PspGI	CCWGG	3 cut(s)	910, 1010, 1926
PspN4I	GGNNCC	6 cut(s)	91, 773, 916, 987, 1994, 2265
PspPI	GGNCC	2 cut(s)	553, 1526
PsuI	RGATCY	3 cut(s)	179, 334, 771
PvuII	CAGCTG	1 cut(s)	1214
RsaI	GTAC	4 cut(s)	197, 1179, 1381, 1758
RsaNI	GTAC	4 cut(s)	196, 1178, 1380, 1757
RseI	CAYNNNNRTG	3 cut(s)	852, 864, 2199
SapI	GCTCTTC	1 cut(s)	1071
Sau3AI	GATC	9 cut(s)	31, 179, 299, 334, 771, 1228, 1564, 1780, 2251
Sau96I	GGNCC	2 cut(s)	553, 1526
SchI	GAGTC	2 cut(s)	1045, 1052
ScrFI	CCNGG	3 cut(s)	912, 1012, 1928
SduI	GDGCHC	4 cut(s)	92, 742, 859, 2266
SfaNI	GCATC	6 cut(s)	495, 1066, 1456, 1669, 1737, 2203
SfcI	CTRYAG	1 cut(s)	1716
Sfr274I	CTCGAG	2 cut(s)	27, 756
SfuI	TTCGAA	1 cut(s)	1801
SinI	GGWCC	2 cut(s)	553, 1526
SlaI	CTCGAG	2 cut(s)	27, 756
SmiMI	CAYNNNNRTG	3 cut(s)	852, 864, 2199
SmlI	CTYRAG	6 cut(s)	27, 161, 756, 1268, 1286, 1325
SmoI	CTYRAG	6 cut(s)	27, 161, 756, 1268, 1286, 1325
SpeI	ACTAGT	2 cut(s)	814, 944
SphI	GCATGC	1 cut(s)	2170
SsiI	CCGC	4 cut(s)	636, 736, 1894, 2183
SspMI	CTAG	7 cut(s)	815, 945, 1070, 1086, 1181, 1451, 2171
StyD4I	CCNGG	3 cut(s)	910, 1010, 1926
StyI	CCWWGG	2 cut(s)	1053, 2143
TaaI	ACNGT	7 cut(s)	140, 460, 547, 1870, 1901, 2130, 2268
TaiI	ACGT	2 cut(s)	1740, 2112
TaqI	TCGA	7 cut(s)	28, 302, 757, 949, 1039, 1801, 1908
TaqII	GACCGA	1 cut(s)	276
TatI	WGTACW	1 cut(s)	1177
TauI	GCSGC	1 cut(s)	1896
TfiI	GAWTC	1 cut(s)	1151
TscAI	CASTG	5 cut(s)	1086, 1213, 1875, 1904, 2223
TseFI	GTSAC	3 cut(s)	278, 1439, 1573
TseI	GCWGC	9 cut(s)	752, 842, 907, 1211, 1214, 1250, 1623, 1750, 2139
Tsp45I	GTSAC	3 cut(s)	278, 1439, 1573
TspGWI	ACGGA	1 cut(s)	1403
TspRI	CASTG	5 cut(s)	1086, 1213, 1875, 1904, 2223
Vha464I	CTTAAG	2 cut(s)	1268, 1325
VpaK11BI	GGWCC	2 cut(s)	553, 1526
XapI	RAATTY	5 cut(s)	17, 52, 567, 1797, 1804
XceI	RCATGY	5 cut(s)	869, 1447, 1757, 2170, 2194
XcmI	CCANNNNNNNNNTGG	2 cut(s)	782, 925
XhoI	CTCGAG	2 cut(s)	27, 756
XmnI	GAANNNNTTC	4 cut(s)	1131, 1684, 1797, 2099
XspI	CTAG	7 cut(s)	815, 945, 1070, 1086, 1181, 1451, 2171
Zsp2I	ATGCAT	2 cut(s)	1449, 2196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.