Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0002851

Physical Location & Seq

Genomic Coordinates Reverse (-)

96346634 .. 96352550

Gene Structure 5917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00075700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2472 bp

ATGCTTGCTGGAGTGAAGTTCATACCTCGACACCAGATTGATAAAGATGAGAATTTGAATGCTTCTGCAAAACAGAAACAGAAGAAGAGCTCAGGTAATAAGAAGGGTAAGCGTAGGAGGGAAAGGAGTTCTGGATATAGTAGTTCGGATGGCGAAGAGCTTGAGAGAATAAAGAAGGGATCTAAAAAAAAGTGGTATTCATCGGATGAGTACTCATCATCATCATATTCTTCGGGAAATGAAAGTGAGGAAAGTTCTGACCGAGCTAGAAAGAAGCGTCTGAATAAGAAAAAGAAGAGATCGACTTGCAGTAGAAAAGATTATAGTTCAGAAGATCTTTCATCTGCTTCTGATAGTTATTCTGATAGGAAAGACAAGCGCAAGAAGAGAAGCCGAAAAGATGGAAAGAAAAAGAGTAGAGATAGAAGTCAGGTTGGCATGGAGGATGATGAATATTCTGACGGTGGTAGAGGAACTAGTACGGTGAAAGATGGGGAAATTGTGAGGAAGAAAATTGGGTTGGAGTGGATGCTTAGACCTGAAGGAAAAGAGAGAACACCTACTATTACTGTCGAGGACCAACCAAAAGAAACTCCAGTTGAAGAGAAGGTCAATCCTAGGGAATTAAATCCATATTTTAAGGATGAGGGAAGTGGTTATCCAGAAGACAATGATGAACCTAAAGCTGGTGGGGACCGACTTTTACCTTCCTCTGTTGTTGGGGATGGAGGTGCAAGTTGGAGACTAAAAGCATTGAAGCGGGCAAAAGAGCAAGCAACTCGAGAAGGACAAGGGCTCCACGAGGTTGTTGAGGAGCGTTGGGGTTCTCTTGGTAAACTAGCGGCCTCTGTGGGATCAAATAAAGCAGCCCCATCTCGTGCTCATCTACATGCAATACAAAGTAGAAAGAAAGGGGTGAGTGAGGAACAGCAGCCAGGTTCCAACAATGACATAGATAAGAAGGTATATGGGAATAACCTAGAGCTTTTTATTTTTGGAAAGTTTGTAACGGTGAAATGTGAAGTAAAGTTTATAAGCCATATGTTAGGTAATAGGCAATGTTGCGGTCTTTCTTACAAGCTACATGTATGTGGCTACCAGGACACTAGCCGGCCCTACCTAAAGGATGTATCTATCCGACATCCCAAAATGAAGGAGCCTAAAGTTCAAGGTTCTTTATCCTGGGGAAAGCAAAGGGGTCAAAAGAGTCGGGATGTCTCTACCAAGGACTCTGCTCTTCTAGCGGATGCAGTGTCTAGCATAAACAGGTTTTCCAATGATGGAAGCTTCCTGAGTGAACTTATTCGTAAGCAGACTAATGATTCTAGGAATCCTGTTAACGAAAATGTACTCGACACGAGAAAACCCAGCGAAGCCAGTGCTGCTGCCTCTAAGGGTGAACTGAGTGCGAACCAGTTGGCAGCTAAGGCTTTTCAGCTTCGTATGAAAGGGAAGCATGAAGAAGCTGAAGAACTTCTGCAAGAAGTAGAAAACCTAAAGGCAAAGCAGGGTGGTGGAGATAATCCGATGAGACCACAATATGAAAGAGGTACAAACAGATATGCTATGCAGGATATGTCTCTTCGTCAAAAGAAAAAGGATGATGATGGTGACATGCATCTAGCCCAAAAGATAATGCAGAACAAAAAATATAGTATATCCGGTCAGGCAGATGATGAATACGACTTTGATGATGGTCCAATCAGAAAGTCTAAAAAGAAGCAGGGAAGTGATGATCGCAAGGTAACCCAGAAGAATACATTTGCAAATCGTTTCTCCATTCAGCAAGAACGCTGCCTTTTCTGCTTTGAAAATCCAAAGAGACCAGCGCATCTGGTTGTGGCAATTGGAAATTATTCATATTTGATGTTGCCACAGCAGAAGCCTGTTGTGGATGGCCATTGCTACATTTTGCCGATGCAGCATGTACCAGCCACAAGAACTCTTGATGATCATGTGTGGGAAGAAATTCGAAATTTCAAGAAATGCCTTATTATGATGTTTGCGAAGCAAGAGAAGGATGTTGTCTTCCTTGAAACAGTGATGGGGTTAGCTCAACAGCGCCACCACTGTATAATTGAGTGTATTCCTTTGCCCCAGGATATAGCTAAAGACGCTCCCTTATATTTTAAAAAGGCAATTGATGAAGCTGAAGATGAGTGGAGCCAACACAATGCCAAAAAACTTATTGATACAAGTATAAAGGGATTGCGAGGTTCAGTTCCAGCTAATTTTCCATATTTCCACGTGGAGTTTGGTTTAAAGAAGGGGTTTGTCCACGTGATTGATGATGAAACACAGTTCAATAGCAGCCTTGGCCTGAATGTTATAAGAGGCATGCTAGAGTTGCCTGCAGAAGACATGCATCGCCGTGGACGATATGAACCAGTGGAAGCGCAAAAGCAAGCCGTTAAGAGGTTTGATCAAGATTGGGCGCCGTTTGACTGGACAAAACAGCTTCATCAATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CwfJ_C_1 CwfJ_C_2

Protein Analysis

823

Amino Acids

93.48

Weight (kDa)

9.33

Isoelectric Point (pI)

44.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CwfJ_C_1	PF04677	591 - 711	2.1e-42	Protein similar to CwfJ C-terminal
CwfJ_C_2	PF04676	720 - 817	1e-26	Protein similar to CwfJ C-terminus 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56290
fragaria_vesca	FvH4_4g02820 FvH4_4g02820 FvH4_4g02820 FvH4_4g02820
malus_domestica	MD13G1208100.v1.1 MD16G1209600.v1.1
prunus_persica	Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1
pyrus_communis	pycom13g18020 pycom16g17690
rosa_chinensis	RchiOBHm_Chr4g0390791 RchiOBHm_Chr5g0000461
rosa_laevigata	RLG00000009911 RLG00000030869
rosa_multiflora	Rmu_sc0000171.1_g000026 Rmu_sc0001380.1_g000027 Rmu_sc0003949.1_g000006
rosa_roxburghii	Rroxscaffold_1G00075700 Rroxscaffold_5G00336660 Rroxscaffold_5G00336680 Rroxscaffold_5G00336690
rosa_rugosa	Rorug03G0331500 Rorug03G0331500 Rorug04G0383800
rosa_samantha	Rh4AG033200 Rh4BG027000 Rh4CG036800 Rh4DG030000 Rh5AG003800 Rh5BG005000 Rh5CG004100 Rh5DG004000
rosa_wichuraiana	Rw5G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1034, 2330
AccB1I	GGYRCC	1 cut(s)	2434
AciI	CCGC	4 cut(s)	760, 842, 1065, 1244
AclWI	GGATC	2 cut(s)	187, 862
AcoI	YGGCCR	1 cut(s)	1897
AcsI	RAATTY	3 cut(s)	52, 1968, 1975
AcuI	CTGAAG	3 cut(s)	561, 1488, 2172
AcvI	CACGTG	2 cut(s)	2248, 2281
AcyI	GRCGYC	1 cut(s)	2435
AfaI	GTAC	5 cut(s)	212, 481, 1350, 1552, 1929
AfiI	CCNNNNNNNGG	2 cut(s)	686, 2415
AflIII	ACRYGT	1 cut(s)	1084
AgsI	TTSAA	8 cut(s)	58, 602, 757, 1169, 1811, 1981, 2036, 2305
AhlI	ACTAGT	1 cut(s)	476
AjnI	CCWGG	4 cut(s)	934, 1098, 1181, 2097
AjuI	GAANNNNNNNTTGG	2 cut(s)	503, 535
Alw21I	GWGCWC	2 cut(s)	92, 883
Alw26I	GTCTC	5 cut(s)	736, 1222, 1525, 1584, 1816
AlwI	GGATC	2 cut(s)	187, 862
Ama87I	CYCGRG	1 cut(s)	780
AoxI	GGCC	4 cut(s)	843, 1112, 1897, 2317
ApeKI	GCWGC	8 cut(s)	866, 931, 1382, 1385, 1421, 1794, 1921, 2310
ApoI	RAATTY	3 cut(s)	52, 1968, 1975
Asp700I	GAANNNNTTC	4 cut(s)	1302, 1473, 1855, 1968
AspA2I	CCTAGG	1 cut(s)	617
AspLEI	GCGC	5 cut(s)	381, 1831, 2064, 2398, 2437
AspS9I	GGNCC	4 cut(s)	577, 694, 1113, 1697
AsuHPI	GGTGA	5 cut(s)	496, 928, 1024, 1409, 1622
AsuII	TTCGAA	1 cut(s)	1972
AvaI	CYCGRG	1 cut(s)	780
AvaII	GGWCC	3 cut(s)	577, 694, 1697
AvrII	CCTAGG	1 cut(s)	617
BalI	TGGCCA	1 cut(s)	1899
BanI	GGYRCC	1 cut(s)	2434
BanII	GRGCYC	2 cut(s)	92, 798
BauI	CACGAG	3 cut(s)	800, 876, 1357
BbrPI	CACGTG	2 cut(s)	2248, 2281
BbsI	GAAGAC	3 cut(s)	672, 2020, 2364
Bbv12I	GWGCWC	2 cut(s)	92, 883
BbvI	GCAGC	8 cut(s)	878, 943, 1369, 1372, 1433, 1781, 1933, 2322
BceAI	ACGGC	3 cut(s)	2355, 2393, 2422
BcgI	CGANNNNNNTGC	4 cut(s)	761, 795, 1361, 1395
BciT130I	CCWGG	4 cut(s)	936, 1100, 1183, 2099
BclI	TGATCA	2 cut(s)	1951, 2422
BcoDI	GTCTC	5 cut(s)	736, 1222, 1525, 1584, 1816
BcuI	ACTAGT	1 cut(s)	476
BfmI	CTRYAG	1 cut(s)	2352
BfoI	RGCGCY	2 cut(s)	2065, 2438
BglII	AGATCT	1 cut(s)	334
BisI	GCNGC	9 cut(s)	843, 867, 932, 1383, 1386, 1422, 1795, 1922, 2311
BlnI	CCTAGG	1 cut(s)	617
BlsI	GCNGC	9 cut(s)	844, 868, 933, 1384, 1387, 1423, 1796, 1923, 2312
BmcAI	AGTACT	1 cut(s)	212
Bme1390I	CCNGG	4 cut(s)	936, 1100, 1183, 2099
Bme18I	GGWCC	3 cut(s)	577, 694, 1697
BmeT110I	CYCGRG	1 cut(s)	780
BmgT120I	GGNCC	4 cut(s)	577, 694, 1113, 1697
BmiI	GGNNCC	6 cut(s)	695, 797, 940, 1158, 2165, 2436
BmrFI	CCNGG	4 cut(s)	936, 1100, 1183, 2099
BmsI	GCATC	6 cut(s)	519, 1237, 1627, 1840, 1908, 2374
BpiI	GAAGAC	3 cut(s)	672, 2020, 2364
BpmI	CTGGAG	2 cut(s)	30, 579
Bpu10I	CCTNAGC	2 cut(s)	91, 1425
Bpu14I	TTCGAA	1 cut(s)	1972
BpuEI	CTTGAG	1 cut(s)	182
BsaAI	YACGTR	2 cut(s)	2248, 2281
BsaHI	GRCGYC	1 cut(s)	2435
BsaI	GGTCTC	2 cut(s)	1525, 1816
BsaJI	CCNNGG	6 cut(s)	617, 1182, 1224, 2097, 2314, 2371
BsaWI	WCCGGW	1 cut(s)	1661
BsaXI	ACNNNNNCTCC	2 cut(s)	780, 810
Bsc4I	CCNNNNNNNGG	2 cut(s)	686, 2415
Bse118I	RCCGGY	1 cut(s)	1110
Bse1I	ACTGG	5 cut(s)	596, 1377, 1414, 2387, 2450
Bse3DI	GCAATG	2 cut(s)	1064, 1900
BseBI	CCWGG	4 cut(s)	936, 1100, 1183, 2099
BseDI	CCNNGG	6 cut(s)	617, 1182, 1224, 2097, 2314, 2371
BseLI	CCNNNNNNNGG	2 cut(s)	686, 2415
BseMI	GCAATG	2 cut(s)	1064, 1900
BseMII	CTCAG	3 cut(s)	105, 1283, 1394
BseNI	ACTGG	5 cut(s)	596, 1377, 1414, 2387, 2450
BseRI	GAGGAG	1 cut(s)	827
BseXI	GCAGC	8 cut(s)	878, 943, 1369, 1372, 1433, 1781, 1933, 2322
BseYI	CCCAGC	1 cut(s)	1367
BshFI	GGCC	4 cut(s)	845, 1114, 1899, 2319
BshNI	GGYRCC	1 cut(s)	2434
BsiHKAI	GWGCWC	2 cut(s)	92, 883
BsiHKCI	CYCGRG	1 cut(s)	780
BsiSI	CCGG	2 cut(s)	1111, 1662
BslFI	GGGAC	1 cut(s)	707
BslI	CCNNNNNNNGG	2 cut(s)	686, 2415
BsmAI	GTCTC	5 cut(s)	736, 1222, 1525, 1584, 1816
BsmFI	GGGAC	1 cut(s)	707
BsmI	GAATGC	1 cut(s)	64
BsnI	GGCC	4 cut(s)	845, 1114, 1899, 2319
Bso31I	GGTCTC	2 cut(s)	1525, 1816
BsoBI	CYCGRG	1 cut(s)	780
Bsp119I	TTCGAA	1 cut(s)	1972
Bsp1286I	GDGCHC	3 cut(s)	92, 798, 883
Bsp143I	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
BspACI	CCGC	4 cut(s)	760, 842, 1065, 1244
BspANI	GGCC	4 cut(s)	845, 1114, 1899, 2319
BspCNI	CTCAG	3 cut(s)	104, 1284, 1395
BspLI	GGNNCC	6 cut(s)	695, 797, 940, 1158, 2165, 2436
BspMAI	CTGCAG	1 cut(s)	2356
BspPI	GGATC	2 cut(s)	187, 862
BspQI	GCTCTTC	3 cut(s)	80, 150, 1242
BspT104I	TTCGAA	1 cut(s)	1972
BspT107I	GGYRCC	1 cut(s)	2434
BspTNI	GGTCTC	2 cut(s)	1525, 1816
BsrDI	GCAATG	2 cut(s)	1064, 1900
BsrFI	RCCGGY	1 cut(s)	1110
BsrI	ACTGG	5 cut(s)	596, 1377, 1414, 2387, 2450
BssAI	RCCGGY	1 cut(s)	1110
BssECI	CCNNGG	6 cut(s)	617, 1182, 1224, 2097, 2314, 2371
BssMI	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
BssNI	GRCGYC	1 cut(s)	2435
BssSI	CACGAG	3 cut(s)	800, 876, 1357
BssT1I	CCWWGG	3 cut(s)	617, 1224, 2314
Bst2BI	CACGAG	3 cut(s)	800, 876, 1357
Bst2UI	CCWGG	4 cut(s)	936, 1100, 1183, 2099
Bst4CI	ACNGT	7 cut(s)	464, 484, 571, 1012, 2041, 2072, 2301
Bst6I	CTCTTC	7 cut(s)	80, 150, 290, 380, 597, 1242, 1587
BstACI	GRCGYC	1 cut(s)	2435
BstBAI	YACGTR	2 cut(s)	2248, 2281
BstBI	TTCGAA	1 cut(s)	1972
BstC8I	GCNNGC	7 cut(s)	6, 762, 774, 1112, 2339, 2352, 2406
BstDEI	CTNAG	6 cut(s)	91, 533, 1292, 1392, 1403, 1425
BstDSI	CCRYGG	1 cut(s)	2371
BstEII	GGTNACC	1 cut(s)	1744
BstH2I	RGCGCY	2 cut(s)	2065, 2438
BstHHI	GCGC	5 cut(s)	381, 1831, 2064, 2398, 2437
BstKTI	GATC	7 cut(s)	182, 302, 337, 857, 1738, 1954, 2425
BstMAI	GTCTC	5 cut(s)	736, 1222, 1525, 1584, 1816
BstMBI	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
BstMWI	GCNNNNNNNGC	8 cut(s)	1241, 1382, 1427, 1803, 1921, 2114, 2316, 2347
BstNI	CCWGG	4 cut(s)	936, 1100, 1183, 2099
BstNSI	RCATGY	6 cut(s)	893, 1088, 1618, 1928, 2341, 2365
BstPI	GGTNACC	1 cut(s)	1744
BstSCI	CCNGG	4 cut(s)	934, 1098, 1181, 2097
BstSFI	CTRYAG	1 cut(s)	2352
BstV1I	GCAGC	8 cut(s)	878, 943, 1369, 1372, 1433, 1781, 1933, 2322
BstV2I	GAAGAC	3 cut(s)	672, 2020, 2364
BstX2I	RGATCY	2 cut(s)	179, 334
BstYI	RGATCY	2 cut(s)	179, 334
BsuRI	GGCC	4 cut(s)	845, 1114, 1899, 2319
BtgI	CCRYGG	1 cut(s)	2371
BtgZI	GCGATG	1 cut(s)	2351
BtsI	GCAGTG	1 cut(s)	1257
BtsIMutI	CAGTG	5 cut(s)	1257, 1384, 2046, 2068, 2394
Cac8I	GCNNGC	7 cut(s)	6, 762, 774, 1112, 2339, 2352, 2406
CfoI	GCGC	5 cut(s)	381, 1831, 2064, 2398, 2437
Cfr10I	RCCGGY	1 cut(s)	1110
Cfr13I	GGNCC	4 cut(s)	577, 694, 1113, 1697
CseI	GACGC	2 cut(s)	266, 2123
Csp6I	GTAC	5 cut(s)	211, 480, 1349, 1551, 1928
CviAII	CATG	9 cut(s)	439, 890, 1085, 1457, 1615, 1925, 1955, 2338, 2362
CviQI	GTAC	5 cut(s)	211, 480, 1349, 1551, 1928
DdeI	CTNAG	6 cut(s)	91, 533, 1292, 1392, 1403, 1425
DinI	GGCGCC	1 cut(s)	2436
DpnI	GATC	7 cut(s)	181, 301, 336, 856, 1737, 1953, 2424
DpnII	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
DraI	TTTAAA	2 cut(s)	2131, 2262
EaeI	YGGCCR	1 cut(s)	1897
Eam1104I	CTCTTC	7 cut(s)	80, 150, 290, 380, 597, 1242, 1587
EarI	CTCTTC	7 cut(s)	80, 150, 290, 380, 597, 1242, 1587
Ecl136II	GAGCTC	1 cut(s)	90
Eco130I	CCWWGG	3 cut(s)	617, 1224, 2314
Eco24I	GRGCYC	2 cut(s)	92, 798
Eco31I	GGTCTC	2 cut(s)	1525, 1816
Eco47I	GGWCC	3 cut(s)	577, 694, 1697
Eco53kI	GAGCTC	1 cut(s)	90
Eco57I	CTGAAG	3 cut(s)	561, 1488, 2172
Eco72I	CACGTG	2 cut(s)	2248, 2281
Eco88I	CYCGRG	1 cut(s)	780
Eco91I	GGTNACC	1 cut(s)	1744
EcoICRI	GAGCTC	1 cut(s)	90
EcoO65I	GGTNACC	1 cut(s)	1744
EcoRII	CCWGG	4 cut(s)	934, 1098, 1181, 2097
EcoT14I	CCWWGG	3 cut(s)	617, 1224, 2314
EcoT22I	ATGCAT	2 cut(s)	1620, 2367
EcoT38I	GRGCYC	2 cut(s)	92, 798
EgeI	GGCGCC	1 cut(s)	2436
EheI	GGCGCC	1 cut(s)	2436
ErhI	CCWWGG	3 cut(s)	617, 1224, 2314
FaeI	CATG	9 cut(s)	442, 893, 1088, 1460, 1618, 1928, 1958, 2341, 2365
FaqI	GGGAC	1 cut(s)	707
FatI	CATG	9 cut(s)	438, 889, 1084, 1456, 1614, 1924, 1954, 2337, 2361
FauI	CCCGC	1 cut(s)	753
FauNDI	CATATG	1 cut(s)	1041
FbaI	TGATCA	2 cut(s)	1951, 2422
Fnu4HI	GCNGC	9 cut(s)	843, 867, 932, 1383, 1386, 1422, 1795, 1922, 2311
FriOI	GRGCYC	2 cut(s)	92, 798
Fsp4HI	GCNGC	9 cut(s)	843, 867, 932, 1383, 1386, 1422, 1795, 1922, 2311
GlaI	GCGC	5 cut(s)	380, 1830, 2063, 2397, 2436
GluI	GCNGC	9 cut(s)	843, 867, 932, 1383, 1386, 1422, 1795, 1922, 2311
GsaI	CCCAGC	1 cut(s)	1371
GsuI	CTGGAG	2 cut(s)	30, 579
HaeII	RGCGCY	2 cut(s)	2065, 2438
HaeIII	GGCC	4 cut(s)	845, 1114, 1899, 2319
HapII	CCGG	2 cut(s)	1111, 1662
HgaI	GACGC	2 cut(s)	266, 2123
HhaI	GCGC	5 cut(s)	381, 1831, 2064, 2398, 2437
Hin1I	GRCGYC	1 cut(s)	2435
Hin1II	CATG	9 cut(s)	442, 893, 1088, 1460, 1618, 1928, 1958, 2341, 2365
Hin6I	GCGC	5 cut(s)	379, 1829, 2062, 2396, 2435
HinP1I	GCGC	5 cut(s)	379, 1829, 2062, 2396, 2435
HincII	GTYRAC	1 cut(s)	1339
HindII	GTYRAC	1 cut(s)	1339
HindIII	AAGCTT	1 cut(s)	1285
HinfI	GANTC	4 cut(s)	1207, 1229, 1322, 1330
HpaI	GTTAAC	1 cut(s)	1339
HpaII	CCGG	2 cut(s)	1111, 1662
HphI	GGTGA	5 cut(s)	496, 928, 1024, 1409, 1622
Hpy166II	GTNNAC	6 cut(s)	836, 1298, 1339, 1400, 2278, 2375
Hpy188III	TCNNGA	9 cut(s)	132, 234, 662, 782, 1211, 1291, 1946, 1981, 2426
Hpy8I	GTNNAC	6 cut(s)	836, 1298, 1339, 1400, 2278, 2375
HpyCH4III	ACNGT	7 cut(s)	464, 484, 571, 1012, 2041, 2072, 2301
HpyCH4IV	ACGT	2 cut(s)	2247, 2280
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1241, 1382, 1427, 1803, 1921, 2114, 2316, 2347
HpyF3I	CTNAG	6 cut(s)	91, 533, 1292, 1392, 1403, 1425
HpySE526I	ACGT	2 cut(s)	2247, 2280
Hsp92I	GRCGYC	1 cut(s)	2435
Hsp92II	CATG	9 cut(s)	442, 893, 1088, 1460, 1618, 1928, 1958, 2341, 2365
HspAI	GCGC	5 cut(s)	379, 1829, 2062, 2396, 2435
KasI	GGCGCC	1 cut(s)	2434
KroI	GCCGGC	1 cut(s)	1110
KroNI	GCCGGC	1 cut(s)	1112
Ksp22I	TGATCA	2 cut(s)	1951, 2422
KspAI	GTTAAC	1 cut(s)	1339
Kzo9I	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
LguI	GCTCTTC	3 cut(s)	80, 150, 1242
LmnI	GCTCC	5 cut(s)	801, 814, 1156, 2122, 2163
Lsp1109I	GCAGC	8 cut(s)	878, 943, 1369, 1372, 1433, 1781, 1933, 2322
LweI	GCATC	6 cut(s)	519, 1237, 1627, 1840, 1908, 2374
MaeII	ACGT	2 cut(s)	2247, 2280
MaeIII	GTNAC	3 cut(s)	1006, 1610, 1744
MalI	GATC	7 cut(s)	181, 301, 336, 856, 1737, 1953, 2424
MboI	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
MfeI	CAATTG	2 cut(s)	1845, 2139
MflI	RGATCY	2 cut(s)	179, 334
MhlI	GDGCHC	3 cut(s)	92, 798, 883
MlsI	TGGCCA	1 cut(s)	1899
MluNI	TGGCCA	1 cut(s)	1899
Mly113I	GGCGCC	1 cut(s)	2435
MlyI	GAGTC	2 cut(s)	1216, 1223
MmeI	TCCRAC	4 cut(s)	501, 719, 966, 1162
Mox20I	TGGCCA	1 cut(s)	1899
Mph1103I	ATGCAT	2 cut(s)	1620, 2367
MroNI	GCCGGC	1 cut(s)	1110
MroXI	GAANNNNTTC	4 cut(s)	1302, 1473, 1855, 1968
MscI	TGGCCA	1 cut(s)	1899
MseI	TTAA	6 cut(s)	626, 639, 1338, 2130, 2261, 2412
MslI	CAYNNNNRTG	3 cut(s)	888, 1089, 2370
Msp20I	TGGCCA	1 cut(s)	1899
MspI	CCGG	2 cut(s)	1111, 1662
MspR9I	CCNGG	4 cut(s)	936, 1100, 1183, 2099
MunI	CAATTG	2 cut(s)	1845, 2139
Mva1269I	GAATGC	1 cut(s)	64
MvaI	CCWGG	4 cut(s)	936, 1100, 1183, 2099
MwoI	GCNNNNNNNGC	8 cut(s)	1241, 1382, 1427, 1803, 1921, 2114, 2316, 2347
NaeI	GCCGGC	1 cut(s)	1112
NarI	GGCGCC	1 cut(s)	2435
NdeI	CATATG	1 cut(s)	1041
NdeII	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
NgoMIV	GCCGGC	1 cut(s)	1110
NlaIII	CATG	9 cut(s)	442, 893, 1088, 1460, 1618, 1928, 1958, 2341, 2365
NlaIV	GGNNCC	6 cut(s)	695, 797, 940, 1158, 2165, 2436
NmuCI	GTSAC	1 cut(s)	1610
NsiI	ATGCAT	2 cut(s)	1620, 2367
NspI	RCATGY	6 cut(s)	893, 1088, 1618, 1928, 2341, 2365
NspV	TTCGAA	1 cut(s)	1972
PaeI	GCATGC	1 cut(s)	2341
PaeR7I	CTCGAG	1 cut(s)	780
PciI	ACATGT	1 cut(s)	1084
PciSI	GCTCTTC	3 cut(s)	80, 150, 1242
PcsI	WCGNNNNNNNCGW	1 cut(s)	2374
PctI	GAATGC	1 cut(s)	64
PdiI	GCCGGC	1 cut(s)	1112
PdmI	GAANNNNTTC	4 cut(s)	1302, 1473, 1855, 1968
PfeI	GAWTC	2 cut(s)	1322, 1330
PkrI	GCNGC	9 cut(s)	844, 868, 933, 1384, 1387, 1423, 1796, 1923, 2312
PleI	GAGTC	2 cut(s)	1215, 1223
PluTI	GGCGCC	1 cut(s)	2438
PmaCI	CACGTG	2 cut(s)	2248, 2281
PmlI	CACGTG	2 cut(s)	2248, 2281
PpsI	GAGTC	2 cut(s)	1215, 1223
Ppu21I	YACGTR	2 cut(s)	2248, 2281
PscI	ACATGT	1 cut(s)	1084
PsiI	TTATAA	2 cut(s)	1034, 2330
Psp124BI	GAGCTC	1 cut(s)	92
Psp6I	CCWGG	4 cut(s)	934, 1098, 1181, 2097
PspCI	CACGTG	2 cut(s)	2248, 2281
PspEI	GGTNACC	1 cut(s)	1744
PspFI	CCCAGC	1 cut(s)	1367
PspGI	CCWGG	4 cut(s)	934, 1098, 1181, 2097
PspN4I	GGNNCC	6 cut(s)	695, 797, 940, 1158, 2165, 2436
PspPI	GGNCC	4 cut(s)	577, 694, 1113, 1697
PstI	CTGCAG	1 cut(s)	2356
PsuI	RGATCY	2 cut(s)	179, 334
RsaI	GTAC	5 cut(s)	212, 481, 1350, 1552, 1929
RsaNI	GTAC	5 cut(s)	211, 480, 1349, 1551, 1928
RseI	CAYNNNNRTG	3 cut(s)	888, 1089, 2370
SacI	GAGCTC	1 cut(s)	92
SapI	GCTCTTC	3 cut(s)	80, 150, 1242
SaqAI	TTAA	6 cut(s)	626, 639, 1338, 2130, 2261, 2412
SatI	GCNGC	9 cut(s)	843, 867, 932, 1383, 1386, 1422, 1795, 1922, 2311
Sau3AI	GATC	7 cut(s)	179, 299, 334, 854, 1735, 1951, 2422
Sau96I	GGNCC	4 cut(s)	577, 694, 1113, 1697
ScaI	AGTACT	1 cut(s)	212
SchI	GAGTC	2 cut(s)	1216, 1223
ScrFI	CCNGG	4 cut(s)	936, 1100, 1183, 2099
SduI	GDGCHC	3 cut(s)	92, 798, 883
SfaNI	GCATC	6 cut(s)	519, 1237, 1627, 1840, 1908, 2374
SfcI	CTRYAG	1 cut(s)	2352
SfoI	GGCGCC	1 cut(s)	2436
Sfr274I	CTCGAG	1 cut(s)	780
SfuI	TTCGAA	1 cut(s)	1972
SinI	GGWCC	3 cut(s)	577, 694, 1697
SlaI	CTCGAG	1 cut(s)	780
SmiMI	CAYNNNNRTG	3 cut(s)	888, 1089, 2370
SmlI	CTYRAG	2 cut(s)	161, 780
SmoI	CTYRAG	2 cut(s)	161, 780
SpeI	ACTAGT	1 cut(s)	476
SphI	GCATGC	1 cut(s)	2341
SsiI	CCGC	4 cut(s)	760, 842, 1065, 1244
SspDI	GGCGCC	1 cut(s)	2434
SspI	AATATT	1 cut(s)	455
SstI	GAGCTC	1 cut(s)	92
StyD4I	CCNGG	4 cut(s)	934, 1098, 1181, 2097
StyI	CCWWGG	3 cut(s)	617, 1224, 2314
TaaI	ACNGT	7 cut(s)	464, 484, 571, 1012, 2041, 2072, 2301
TaiI	ACGT	2 cut(s)	2250, 2283
TaqI	TCGA	6 cut(s)	28, 302, 573, 781, 1353, 1972
TaqII	GACCGA	2 cut(s)	276, 711
TatI	WGTACW	2 cut(s)	210, 1348
TauI	GCSGC	1 cut(s)	845
TfiI	GAWTC	2 cut(s)	1322, 1330
Tru1I	TTAA	6 cut(s)	626, 639, 1338, 2130, 2261, 2412
Tru9I	TTAA	6 cut(s)	626, 639, 1338, 2130, 2261, 2412
TscAI	CASTG	5 cut(s)	1257, 1384, 2046, 2075, 2394
TseFI	GTSAC	1 cut(s)	1610
TseI	GCWGC	8 cut(s)	866, 931, 1382, 1385, 1421, 1794, 1921, 2310
Tsp45I	GTSAC	1 cut(s)	1610
TspRI	CASTG	5 cut(s)	1257, 1384, 2046, 2075, 2394
VpaK11BI	GGWCC	3 cut(s)	577, 694, 1697
XapI	RAATTY	3 cut(s)	52, 1968, 1975
XceI	RCATGY	6 cut(s)	893, 1088, 1618, 1928, 2341, 2365
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2252
XhoI	CTCGAG	1 cut(s)	780
XmaJI	CCTAGG	1 cut(s)	617
XmnI	GAANNNNTTC	4 cut(s)	1302, 1473, 1855, 1968
ZrmI	AGTACT	1 cut(s)	212
Zsp2I	ATGCAT	2 cut(s)	1620, 2367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.