Rh5BG005000

CWF19-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002851
Physical Location & Seq
Forward (+)
402773 .. 410089
7317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG005000.1

Sequence Viewer

Length: 1794 bp
ATGCTTGCTGGAGTGAAGTTCATACCTCGAGACCAGATTCATAAAGATGAGAATTTGAATGCTTCTGCAAAACAGAAACAGAAGAAGAGCTCAGGTAATAAGAAGGTTAAGCGTAGGAGGAGGAGGGAAAGGAGTTCTGGATATACTAGTTCAGATGGCGAAGAGCTTGAGAGAATAAAGAAGGGATCTAAAAAAAAGTGGTATTCATCGGATGAGTACTCATCATCATCATATTCTTCGGGAAATGAAAGTGAGGAAAGTTCTGACCGAGATAGAAAGAAGCGTCTGAATAAGAAAAAGAAGAGATCGAGTAGCAGTAGAAAAGATTATAGTTCAGAAGATCTTTCATCTGCTTCTGATAGGAAAGACAAGCGCAAGAAGAGAAGCCGAAAAGATGGAAAGAAAAAGAGTAGAGATACAAGTCAGGGTGGCATGGAGGATGATGAATATTCTGACGGTGGTAGAGGAACTAGTACGGTGAAAGATGGGGAAATTGTGAGGAAGAAAATTGGGTTGGAGTGGATGCTTAGACCTGAAGGAAAAGAGAGAACACCTACTATTACTGTCGAGGACCAACCAAAAGAAACTCCAGTTGAAGAGAAGGTCAATCCTAGGGAATTAAATCCATATTTTAAGGATGAGGGAAGTGGTTATCCAGAAGACAATGATGAACCTAAAGCTGGTGGGGACCGACTTTTACCTTCCTCTGTTGTTGGGGATGGAGGTGCAAGTTGGAGACTTAAAGCATTGAAGCGGGCAAAAGAGCAAGCAACTCGAGAAGGACAAGGGCTCCACGAGGTTGTTGAGGAGCGTTGGGGTTCTCTTGGTAAACTAGCGGCCTCTGTGGGATCAAATAAAGCAGCCCCATCTCGTGCTCATCTACATGCAATACAAAGTAGAAAGAAAGGGGTGAGTGAGGAACAGCAGCCAGGCTCCAACAATGACATAGATAAGAAGGACACTAGGCGGCCCTACCTAAAGGATGTATCTATCCGACATCCCAAAATGAAGGAGCCTAAAGTTCAAGGTTCTTTATCCTGGGGAAAGCAAAAGGGTCAAAAGAGTCGGGATGTCTCTACCAAGGACTCTGCTCTTCTAGCGGATGCAGTGTCTAGCATAAACAGGTTTTCCAATGATGGAAGCTTCCTGAGTGAACTTATTCATAAGCAGACTAATGATTCTAGGAATCCTGTTAACGAAAATGTACTCGACACGAGAAAACCCAGCGAAGCCAGTGCTGCTGCCTCTAAGGGTGAACTGAGTGCGAACCAGTTGGCAGCTAAGGCTTTTCAGCTTCGTATGAAAGGGAAGCATGAAGAAGCTGAAGAACTTCTGCAAGAAGTAGAAAACCTAAAGGCAAAGCAGGGTGGTGGAGATAATCCGATGAGACCACAATATGAAAGAGGTACAAACAGATATGCTATGCAGGATATGTCTCTTCGTCAAAAGAAAAAGGATGATGATGGTGACATGCATCTAGCCCAAAAGATAATGCAGAACAAAAAATATAGTATATCCGGTCAGGCAGATGATGAATACGACTTTGATGATGGTCCAATCAGAAAGTCTAAAAAGAAGCAGGGAAGTGATGATCGCAAGGTAACCCAGAAGAATACATTTGCAAATCGTTTCTCCATTCAGCAAGAACGCTGCCTTTTCTGCTTTGAAAATCCAAAGAGACCAGCGCATCTGGTTGCGGCAATTGGAAATTATTCATATTTGATGTTGCCACAGCAGAAGCCTGTTGTGGATGGTCATTGCTACATTTTGCCGATGCAGGTAAGCTGGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CwfJ_C_1 CwfJ_C_2

Protein Analysis

597

Amino Acids

67.34

Weight (kDa)

9.63

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CwfJ_C_1 PF04677 543 - 593 5.3e-13 Protein similar to CwfJ C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1768
AciI CCGC 5 cut(s) 754, 836, 967, 1100, 1697
AclWI GGATC 2 cut(s) 193, 856
AcsI RAATTY 1 cut(s) 52
AcuI CTGAAG 2 cut(s) 555, 1344
AfaI GTAC 4 cut(s) 218, 475, 1206, 1408
AfiI CCNNNNNNNGG 1 cut(s) 680
AgsI TTSAA 5 cut(s) 58, 596, 751, 1025, 1667
AhlI ACTAGT 2 cut(s) 146, 470
AjnI CCWGG 2 cut(s) 928, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 8 cut(s) 90, 166, 680, 1143, 1280, 1294, 1322, 1785
AluI AGCT 8 cut(s) 90, 166, 680, 1143, 1280, 1294, 1322, 1785
Alw21I GWGCWC 2 cut(s) 92, 877
Alw26I GTCTC 6 cut(s) 24, 730, 1078, 1381, 1440, 1672
AlwI GGATC 2 cut(s) 193, 856
Ama87I CYCGRG 2 cut(s) 27, 774
AoxI GGCC 2 cut(s) 837, 968
ApeKI GCWGC 6 cut(s) 860, 925, 1238, 1241, 1277, 1650
ApoI RAATTY 1 cut(s) 52
Asp700I GAANNNNTTC 3 cut(s) 1158, 1329, 1711
AspA2I CCTAGG 1 cut(s) 611
AspLEI GCGC 2 cut(s) 375, 1687
AspS9I GGNCC 4 cut(s) 571, 688, 969, 1553
AsuHPI GGTGA 4 cut(s) 490, 922, 1265, 1478
AvaI CYCGRG 2 cut(s) 27, 774
AvaII GGWCC 3 cut(s) 571, 688, 1553
AvrII CCTAGG 1 cut(s) 611
BanII GRGCYC 2 cut(s) 92, 792
BauI CACGAG 3 cut(s) 794, 870, 1213
BbsI GAAGAC 1 cut(s) 666
Bbv12I GWGCWC 2 cut(s) 92, 877
BbvI GCAGC 6 cut(s) 872, 937, 1225, 1228, 1289, 1637
BccI CCATC 9 cut(s) 149, 389, 479, 713, 874, 1130, 1457, 1544, 1745
BcgI CGANNNNNNTGC 4 cut(s) 755, 789, 1217, 1251
BciT130I CCWGG 2 cut(s) 930, 1039
BcoDI GTCTC 6 cut(s) 24, 730, 1078, 1381, 1440, 1672
BcuI ACTAGT 2 cut(s) 146, 470
BfuAI ACCTGC 1 cut(s) 1768
BglII AGATCT 1 cut(s) 340
BisI GCNGC 9 cut(s) 837, 861, 926, 968, 1239, 1242, 1278, 1651, 1698
BlnI CCTAGG 1 cut(s) 611
BlsI GCNGC 9 cut(s) 838, 862, 927, 969, 1240, 1243, 1279, 1652, 1699
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 2 cut(s) 930, 1039
Bme18I GGWCC 3 cut(s) 571, 688, 1553
BmeT110I CYCGRG 2 cut(s) 27, 774
BmgT120I GGNCC 4 cut(s) 571, 688, 969, 1553
BmiI GGNNCC 4 cut(s) 689, 791, 934, 1014
BmrFI CCNGG 2 cut(s) 930, 1039
BmsI GCATC 5 cut(s) 513, 1093, 1483, 1696, 1764
BpiI GAAGAC 1 cut(s) 666
BpmI CTGGAG 2 cut(s) 30, 573
Bpu10I CCTNAGC 2 cut(s) 91, 1281
BpuEI CTTGAG 1 cut(s) 188
BsaI GGTCTC 3 cut(s) 24, 1381, 1672
BsaJI CCNNGG 3 cut(s) 611, 1038, 1080
BsaWI WCCGGW 1 cut(s) 1517
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bsc4I CCNNNNNNNGG 1 cut(s) 680
Bse1I ACTGG 3 cut(s) 590, 1233, 1270
Bse3DI GCAATG 1 cut(s) 1756
BseBI CCWGG 2 cut(s) 930, 1039
BseDI CCNNGG 3 cut(s) 611, 1038, 1080
BseLI CCNNNNNNNGG 1 cut(s) 680
BseMI GCAATG 1 cut(s) 1756
BseMII CTCAG 3 cut(s) 105, 1139, 1250
BseNI ACTGG 3 cut(s) 590, 1233, 1270
BseRI GAGGAG 3 cut(s) 133, 136, 821
BseXI GCAGC 6 cut(s) 872, 937, 1225, 1228, 1289, 1637
BseYI CCCAGC 1 cut(s) 1223
BshFI GGCC 2 cut(s) 839, 970
BsiHKAI GWGCWC 2 cut(s) 92, 877
BsiHKCI CYCGRG 2 cut(s) 27, 774
BsiSI CCGG 1 cut(s) 1518
BslFI GGGAC 1 cut(s) 701
BslI CCNNNNNNNGG 1 cut(s) 680
BsmAI GTCTC 6 cut(s) 24, 730, 1078, 1381, 1440, 1672
BsmFI GGGAC 1 cut(s) 701
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 2 cut(s) 839, 970
Bso31I GGTCTC 3 cut(s) 24, 1381, 1672
BsoBI CYCGRG 2 cut(s) 27, 774
Bsp1286I GDGCHC 3 cut(s) 92, 792, 877
Bsp143I GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
BspACI CCGC 5 cut(s) 754, 836, 967, 1100, 1697
BspANI GGCC 2 cut(s) 839, 970
BspCNI CTCAG 3 cut(s) 104, 1140, 1251
BspLI GGNNCC 4 cut(s) 689, 791, 934, 1014
BspMI ACCTGC 1 cut(s) 1768
BspPI GGATC 2 cut(s) 193, 856
BspQI GCTCTTC 3 cut(s) 80, 156, 1098
BspTNI GGTCTC 3 cut(s) 24, 1381, 1672
BsrDI GCAATG 1 cut(s) 1756
BsrI ACTGG 3 cut(s) 590, 1233, 1270
BssECI CCNNGG 3 cut(s) 611, 1038, 1080
BssMI GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
BssSI CACGAG 3 cut(s) 794, 870, 1213
BssT1I CCWWGG 2 cut(s) 611, 1080
Bst2BI CACGAG 3 cut(s) 794, 870, 1213
Bst2UI CCWGG 2 cut(s) 930, 1039
Bst4CI ACNGT 3 cut(s) 458, 478, 565
Bst6I CTCTTC 7 cut(s) 80, 156, 296, 374, 591, 1098, 1443
BstC8I GCNNGC 3 cut(s) 6, 756, 768
BstDEI CTNAG 6 cut(s) 91, 527, 1148, 1248, 1259, 1281
BstEII GGTNACC 1 cut(s) 1600
BstHHI GCGC 2 cut(s) 375, 1687
BstKTI GATC 6 cut(s) 188, 308, 343, 851, 1594, 1791
BstMAI GTCTC 6 cut(s) 24, 730, 1078, 1381, 1440, 1672
BstMBI GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
BstMWI GCNNNNNNNGC 4 cut(s) 1097, 1238, 1283, 1659
BstNI CCWGG 2 cut(s) 930, 1039
BstNSI RCATGY 2 cut(s) 887, 1474
BstPI GGTNACC 1 cut(s) 1600
BstSCI CCNGG 2 cut(s) 928, 1037
BstV1I GCAGC 6 cut(s) 872, 937, 1225, 1228, 1289, 1637
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 3 cut(s) 185, 340, 1788
BstYI RGATCY 3 cut(s) 185, 340, 1788
BsuRI GGCC 2 cut(s) 839, 970
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 2 cut(s) 1113, 1240
BveI ACCTGC 1 cut(s) 1768
Cac8I GCNNGC 3 cut(s) 6, 756, 768
CfoI GCGC 2 cut(s) 375, 1687
Cfr13I GGNCC 4 cut(s) 571, 688, 969, 1553
CseI GACGC 1 cut(s) 272
Csp6I GTAC 4 cut(s) 217, 474, 1205, 1407
CviAII CATG 4 cut(s) 433, 884, 1313, 1471
CviQI GTAC 4 cut(s) 217, 474, 1205, 1407
DdeI CTNAG 6 cut(s) 91, 527, 1148, 1248, 1259, 1281
DpnI GATC 6 cut(s) 187, 307, 342, 850, 1593, 1790
DpnII GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
Eam1104I CTCTTC 7 cut(s) 80, 156, 296, 374, 591, 1098, 1443
EarI CTCTTC 7 cut(s) 80, 156, 296, 374, 591, 1098, 1443
Ecl136II GAGCTC 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 611, 1080
Eco24I GRGCYC 2 cut(s) 92, 792
Eco31I GGTCTC 3 cut(s) 24, 1381, 1672
Eco47I GGWCC 3 cut(s) 571, 688, 1553
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 2 cut(s) 555, 1344
Eco88I CYCGRG 2 cut(s) 27, 774
Eco91I GGTNACC 1 cut(s) 1600
EcoICRI GAGCTC 1 cut(s) 90
EcoO65I GGTNACC 1 cut(s) 1600
EcoRII CCWGG 2 cut(s) 928, 1037
EcoT14I CCWWGG 2 cut(s) 611, 1080
EcoT22I ATGCAT 1 cut(s) 1476
EcoT38I GRGCYC 2 cut(s) 92, 792
ErhI CCWWGG 2 cut(s) 611, 1080
FaeI CATG 4 cut(s) 436, 887, 1316, 1474
FaqI GGGAC 1 cut(s) 701
FatI CATG 4 cut(s) 432, 883, 1312, 1470
FauI CCCGC 1 cut(s) 747
Fnu4HI GCNGC 9 cut(s) 837, 861, 926, 968, 1239, 1242, 1278, 1651, 1698
FriOI GRGCYC 2 cut(s) 92, 792
Fsp4HI GCNGC 9 cut(s) 837, 861, 926, 968, 1239, 1242, 1278, 1651, 1698
GlaI GCGC 2 cut(s) 374, 1686
GluI GCNGC 9 cut(s) 837, 861, 926, 968, 1239, 1242, 1278, 1651, 1698
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 2 cut(s) 30, 573
HaeIII GGCC 2 cut(s) 839, 970
HapII CCGG 1 cut(s) 1518
HgaI GACGC 1 cut(s) 272
HhaI GCGC 2 cut(s) 375, 1687
Hin1II CATG 4 cut(s) 436, 887, 1316, 1474
Hin6I GCGC 2 cut(s) 373, 1685
HinP1I GCGC 2 cut(s) 373, 1685
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 1 cut(s) 1141
HinfI GANTC 5 cut(s) 37, 1063, 1085, 1178, 1186
HpaI GTTAAC 1 cut(s) 1195
HpaII CCGG 1 cut(s) 1518
HphI GGTGA 4 cut(s) 490, 922, 1265, 1478
Hpy166II GTNNAC 4 cut(s) 830, 1154, 1195, 1256
Hpy188III TCNNGA 7 cut(s) 29, 138, 240, 656, 776, 1067, 1147
Hpy8I GTNNAC 4 cut(s) 830, 1154, 1195, 1256
HpyAV CCTTC 8 cut(s) 97, 175, 530, 595, 711, 773, 949, 1003
HpyCH4III ACNGT 3 cut(s) 458, 478, 565
HpyF10VI GCNNNNNNNGC 4 cut(s) 1097, 1238, 1283, 1659
HpyF3I CTNAG 6 cut(s) 91, 527, 1148, 1248, 1259, 1281
Hsp92II CATG 4 cut(s) 436, 887, 1316, 1474
HspAI GCGC 2 cut(s) 373, 1685
KspAI GTTAAC 1 cut(s) 1195
Kzo9I GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
LguI GCTCTTC 3 cut(s) 80, 156, 1098
LmnI GCTCC 4 cut(s) 795, 808, 938, 1012
Lsp1109I GCAGC 6 cut(s) 872, 937, 1225, 1228, 1289, 1637
LweI GCATC 5 cut(s) 513, 1093, 1483, 1696, 1764
MaeIII GTNAC 2 cut(s) 1466, 1600
MalI GATC 6 cut(s) 187, 307, 342, 850, 1593, 1790
MboI GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
MfeI CAATTG 1 cut(s) 1701
MflI RGATCY 3 cut(s) 185, 340, 1788
MhlI GDGCHC 3 cut(s) 92, 792, 877
MluCI AATT 6 cut(s) 52, 492, 507, 617, 1701, 1708
MlyI GAGTC 2 cut(s) 1072, 1079
MmeI TCCRAC 4 cut(s) 495, 713, 960, 1018
Mph1103I ATGCAT 1 cut(s) 1476
MroXI GAANNNNTTC 3 cut(s) 1158, 1329, 1711
MseI TTAA 5 cut(s) 108, 620, 633, 741, 1194
MslI CAYNNNNRTG 2 cut(s) 45, 882
MspI CCGG 1 cut(s) 1518
MspR9I CCNGG 2 cut(s) 930, 1039
MunI CAATTG 1 cut(s) 1701
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 2 cut(s) 930, 1039
MwoI GCNNNNNNNGC 4 cut(s) 1097, 1238, 1283, 1659
NdeII GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
NlaIII CATG 4 cut(s) 436, 887, 1316, 1474
NlaIV GGNNCC 4 cut(s) 689, 791, 934, 1014
NmuCI GTSAC 1 cut(s) 1466
NsiI ATGCAT 1 cut(s) 1476
NspI RCATGY 2 cut(s) 887, 1474
PaeR7I CTCGAG 2 cut(s) 27, 774
PciSI GCTCTTC 3 cut(s) 80, 156, 1098
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 3 cut(s) 1158, 1329, 1711
PfeI GAWTC 3 cut(s) 37, 1178, 1186
PkrI GCNGC 9 cut(s) 838, 862, 927, 969, 1240, 1243, 1279, 1652, 1699
PleI GAGTC 2 cut(s) 1071, 1079
PpsI GAGTC 2 cut(s) 1071, 1079
Psp124BI GAGCTC 1 cut(s) 92
Psp6I CCWGG 2 cut(s) 928, 1037
PspEI GGTNACC 1 cut(s) 1600
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 2 cut(s) 928, 1037
PspN4I GGNNCC 4 cut(s) 689, 791, 934, 1014
PspPI GGNCC 4 cut(s) 571, 688, 969, 1553
PsuI RGATCY 3 cut(s) 185, 340, 1788
RsaI GTAC 4 cut(s) 218, 475, 1206, 1408
RsaNI GTAC 4 cut(s) 217, 474, 1205, 1407
RseI CAYNNNNRTG 2 cut(s) 45, 882
SacI GAGCTC 1 cut(s) 92
SapI GCTCTTC 3 cut(s) 80, 156, 1098
SaqAI TTAA 5 cut(s) 108, 620, 633, 741, 1194
SatI GCNGC 9 cut(s) 837, 861, 926, 968, 1239, 1242, 1278, 1651, 1698
Sau3AI GATC 6 cut(s) 185, 305, 340, 848, 1591, 1788
Sau96I GGNCC 4 cut(s) 571, 688, 969, 1553
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 2 cut(s) 1072, 1079
ScrFI CCNGG 2 cut(s) 930, 1039
SduI GDGCHC 3 cut(s) 92, 792, 877
SfaNI GCATC 5 cut(s) 513, 1093, 1483, 1696, 1764
Sfr274I CTCGAG 2 cut(s) 27, 774
SinI GGWCC 3 cut(s) 571, 688, 1553
SlaI CTCGAG 2 cut(s) 27, 774
SmiMI CAYNNNNRTG 2 cut(s) 45, 882
SmlI CTYRAG 3 cut(s) 27, 167, 774
SmoI CTYRAG 3 cut(s) 27, 167, 774
SpeI ACTAGT 2 cut(s) 146, 470
Sse9I AATT 6 cut(s) 52, 492, 507, 617, 1701, 1708
SsiI CCGC 5 cut(s) 754, 836, 967, 1100, 1697
SspI AATATT 1 cut(s) 449
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 2 cut(s) 928, 1037
StyI CCWWGG 2 cut(s) 611, 1080
TaaI ACNGT 3 cut(s) 458, 478, 565
TaqI TCGA 5 cut(s) 28, 308, 567, 775, 1209
TaqII GACCGA 2 cut(s) 282, 705
TasI AATT 6 cut(s) 52, 492, 507, 617, 1701, 1708
TatI WGTACW 2 cut(s) 216, 1204
TauI GCSGC 3 cut(s) 839, 970, 1700
TfiI GAWTC 3 cut(s) 37, 1178, 1186
Tru1I TTAA 5 cut(s) 108, 620, 633, 741, 1194
Tru9I TTAA 5 cut(s) 108, 620, 633, 741, 1194
TscAI CASTG 2 cut(s) 1113, 1240
TseFI GTSAC 1 cut(s) 1466
TseI GCWGC 6 cut(s) 860, 925, 1238, 1241, 1277, 1650
Tsp45I GTSAC 1 cut(s) 1466
TspRI CASTG 2 cut(s) 1113, 1240
VpaK11BI GGWCC 3 cut(s) 571, 688, 1553
XapI RAATTY 1 cut(s) 52
XceI RCATGY 2 cut(s) 887, 1474
XhoI CTCGAG 2 cut(s) 27, 774
XmaJI CCTAGG 1 cut(s) 611
XmnI GAANNNNTTC 3 cut(s) 1158, 1329, 1711
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.