Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0002851

Physical Location & Seq

Genomic Coordinates Forward (+)

276958 .. 283140

Gene Structure 6183 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG004100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGCTTGCTGGACTCAAGTTCATACCTCGAGACCAGATTGATAAAGATGAGAATTTGAATGCTTCTGCAAAACAGAAACAGAAGAAGAGCTCAGGTAATAAGAAGGGTAAGCGTAGGAGCGAAAGGAGTTCTGGATATAGTAGTTCGGATGGCGAAGAGCTTGAGAGAATAAAGAAGGCATCTAAAAAAAAGTGGTATTCATCGGATGAGTACTCATCACCATCATATTCTTCGGGAAATGAAAGTGAGGAAAGTTCTGACCGAGATAGAAAGAAGCGTCTGAATAAGAAAAAGAAGAGATCGAGTAGCAGTAGAAAAGATTATAGTTCAGAAGATCTTTCATCTGCTTCTGATAGTTATTCTGATAGGAAAGACAAGCGCAAGAAGAGAAGCCGAAAAGATGGAAAGAAAAAGAGTAGAGATAGAAGTCAGGGTGGCATGGAGGATGATGAATATTCTGACGGTGGTAGAGGAACTAGTACGGTGAAAGATGGGGAAATTGTGAGGAAGAAAATTGGGTTGGAGTGGATGCTTAGACCTGAAGGAAAAGAGAGAACACCTACTATTACTGTCGAGGACCAACCAAAAGAAACTCCAGTTGAAGAGGTCAATCCTAGGGAATTAAATCCATATTTTAAGGATGAGGGAAGTGGTTATCCAGAAGACAATGATGAACCTAAAGCTGGTGGGGACCGACTTTTACCTTCCTCTGTTGTTGGGGATGGAGGTGCAAGTTGGAGACTTAAAGCATTGAAGCGGGCAAAAGAGCAAGCAACTCGAGAAGGACAAGGGCTCCACGAGGTTGTTGAGGAGCGTTGGGGTTCTCTTGGTAAACTAGCAGCCTCTGTGGGATCAAATAAAGCAGCCCCATCTCGTGCTCATCTACATGCAATACAAAGTAGAAAGAAAGGGGTGAGTGAGGAACAGCAGCCAGGTTCCAACAATGACATAGATAAGAAGGACACTAGCCGGCCCTACCTAAAGGATGTATCTATCCAACATCCCAAAATGAAGGAGCCTAAAGTTCAAGGTTCTTTATCCTGGGGAAAGCAAAAGGGTCAAAAGAGCCGGGATGTCTCTACCAAGGACTCTGCTCTTCTAGCGGATGCAGTGTCTAGCATAAACAGGTTTTCCAATGATGGAAGCTTCCTGAGTGAACTTATTCGTAATCAGACTAATGATTCTAGGAATCCTGTTAACGAAAATGTACTTGACACGAGAAAACCCAGCGAAACCAGTGCTGCTGCCTCTAAGGGTGAACTGAGTGCGAACCAGTTGGCAGCTAAGGCTTTTCAGCTTCGTATGAAGGGGAAGCATGAAGAAGCTGAAGAACTTCTGCAAGAAATAGAAAACCTAAAGGCAAAGCAGGGTGGTGGAGATAATCCGATGAGACCACAATATGAAAGAGGTACAAACAGATATGCCATGCAGGATATGTCTCTTCGTCAAAAGAAAAAGGATGATGATGGTGACATGCATCTAGCCCAAAAGATAATGCAGAACAAAAAATATAGTATATCCGGTCAGGCAGATGATGAATACGACTTTGATGATGGTCCAATCAGAAAGTCTAAAAAGAAGCAGGGAAGTGATGATCGCAAGGTAACCCAGAAGAATACATTTGCAAATCGTTTCTCCATTCAGCAAGAACGCTGCCTTTTCTGCTTTGAAAATCCAAAGAGACCAGCGCATCTGGTTGTGGCAATTGGAAATTATTCATATTTGATGTTGCCACAGCAGAAGCCTGTTGTGGATGGTCATTGCTACATTTTGCCGATGCAGCATGGACCAGCCACAAGAACTCTTGATGATCATGTGTGGGAAGAAATTCGAAATTTCAAGAAATGCCTTATTATGATGTTTGCGAAGCAAGAGAAGGATGTTGTCTTCCTTGAAACAGTGATGGGGTTAGCTCAACAGCGCCGCCACTGTATAATTGAGTGTATTCCTTTGCCCCAGGATATAGCTAAAGACGCTCCCTTATATTTTAAAAAGGCAATTGATGAAGCTGAAGATGAGTGGAGCCAACACAATGCCAAAAAACTTATTGATACAAGTGTAAAGGGATTGCGAGGTTCAGTTCCAGCTAATTTTCCATATTTCCACGTGGAGTTTGGTTTAAAGAAGGGGTTTGTCCACGTGATTGATGATGAAACACAGTTCAATAGCAGCCTTGGCCTGAATGTTATAAGAGGCATGCTAGAGTTGCCTGCAGAAGACATGCATCGCCGTGGACGATATGAACCAGTGGAAGCGCAAAAGCAAGCCGTTAAGAGGTTTGATCAAGATTGGGCGCCGTTTGACTGGACAAAACAGCTTCATCAATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CwfJ_C_1 CwfJ_C_2

Protein Analysis

776

Amino Acids

87.93

Weight (kDa)

9.24

Isoelectric Point (pI)

49.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CwfJ_C_1	PF04677	544 - 664	5.9e-42	Protein similar to CwfJ C-terminal
CwfJ_C_2	PF04676	673 - 770	6e-27	Protein similar to CwfJ C-terminus 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56290
fragaria_vesca	FvH4_4g02820 FvH4_4g02820 FvH4_4g02820 FvH4_4g02820
malus_domestica	MD13G1208100.v1.1 MD16G1209600.v1.1
prunus_persica	Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1
pyrus_communis	pycom13g18020 pycom16g17690
rosa_chinensis	RchiOBHm_Chr4g0390791 RchiOBHm_Chr5g0000461
rosa_laevigata	RLG00000009911 RLG00000030869
rosa_multiflora	Rmu_sc0000171.1_g000026 Rmu_sc0001380.1_g000027 Rmu_sc0003949.1_g000006
rosa_roxburghii	Rroxscaffold_1G00075700 Rroxscaffold_5G00336660 Rroxscaffold_5G00336680 Rroxscaffold_5G00336690
rosa_rugosa	Rorug03G0331500 Rorug03G0331500 Rorug04G0383800
rosa_samantha	Rh4AG033200 Rh4BG027000 Rh4CG036800 Rh4DG030000 Rh5AG003800 Rh5BG005000 Rh5CG004100 Rh5DG004000
rosa_wichuraiana	Rw5G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2189
AccB1I	GGYRCC	1 cut(s)	2293
AciI	CCGC	3 cut(s)	757, 1103, 1924
AclWI	GGATC	1 cut(s)	859
AcsI	RAATTY	3 cut(s)	52, 1827, 1834
AcuI	CTGAAG	3 cut(s)	561, 1347, 2031
AcvI	CACGTG	2 cut(s)	2107, 2140
AcyI	GRCGYC	1 cut(s)	2294
AfaI	GTAC	4 cut(s)	212, 481, 1209, 1411
AfiI	CCNNNNNNNGG	2 cut(s)	683, 2274
AgsI	TTSAA	8 cut(s)	58, 602, 754, 1028, 1670, 1840, 1895, 2164
AhlI	ACTAGT	1 cut(s)	476
AjnI	CCWGG	3 cut(s)	931, 1040, 1956
AjuI	GAANNNNNNNTTGG	2 cut(s)	503, 535
Alw21I	GWGCWC	2 cut(s)	92, 880
Alw26I	GTCTC	6 cut(s)	24, 733, 1081, 1384, 1443, 1675
AlwI	GGATC	1 cut(s)	859
AlwNI	CAGNNNCTG	1 cut(s)	845
Ama87I	CYCGRG	2 cut(s)	27, 777
AoxI	GGCC	2 cut(s)	971, 2176
ApeKI	GCWGC	9 cut(s)	839, 863, 928, 1241, 1244, 1280, 1653, 1780, 2169
ApoI	RAATTY	3 cut(s)	52, 1827, 1834
Asp700I	GAANNNNTTC	4 cut(s)	1161, 1332, 1714, 1827
AspA2I	CCTAGG	1 cut(s)	614
AspLEI	GCGC	5 cut(s)	381, 1690, 1923, 2257, 2296
AspS9I	GGNCC	5 cut(s)	577, 691, 972, 1556, 1787
AsuC2I	CCSGG	1 cut(s)	1070
AsuHPI	GGTGA	5 cut(s)	210, 496, 925, 1268, 1481
AsuII	TTCGAA	1 cut(s)	1831
AvaI	CYCGRG	2 cut(s)	27, 777
AvaII	GGWCC	4 cut(s)	577, 691, 1556, 1787
AvrII	CCTAGG	1 cut(s)	614
BaeI	ACNNNNGTAYC	2 cut(s)	2043, 2076
BanI	GGYRCC	1 cut(s)	2293
BanII	GRGCYC	2 cut(s)	92, 795
BauI	CACGAG	3 cut(s)	797, 873, 1216
BbrPI	CACGTG	2 cut(s)	2107, 2140
BbsI	GAAGAC	3 cut(s)	669, 1879, 2223
Bbv12I	GWGCWC	2 cut(s)	92, 880
BbvI	GCAGC	9 cut(s)	851, 875, 940, 1228, 1231, 1292, 1640, 1792, 2181
BceAI	ACGGC	3 cut(s)	2214, 2252, 2281
BcgI	CGANNNNNNTGC	4 cut(s)	758, 792, 1220, 1254
BciT130I	CCWGG	3 cut(s)	933, 1042, 1958
BclI	TGATCA	2 cut(s)	1810, 2281
BcnI	CCSGG	1 cut(s)	1070
BcoDI	GTCTC	6 cut(s)	24, 733, 1081, 1384, 1443, 1675
BcuI	ACTAGT	1 cut(s)	476
BfaI	CTAG	9 cut(s)	477, 615, 836, 966, 1100, 1116, 1185, 1481, 2201
BfmI	CTRYAG	1 cut(s)	2211
BfoI	RGCGCY	2 cut(s)	1924, 2297
BglII	AGATCT	1 cut(s)	334
BlnI	CCTAGG	1 cut(s)	614
BmcAI	AGTACT	1 cut(s)	212
Bme1390I	CCNGG	4 cut(s)	933, 1042, 1070, 1958
Bme18I	GGWCC	4 cut(s)	577, 691, 1556, 1787
BmeT110I	CYCGRG	2 cut(s)	27, 777
BmgT120I	GGNCC	5 cut(s)	577, 691, 972, 1556, 1787
BmiI	GGNNCC	6 cut(s)	692, 794, 937, 1017, 2024, 2295
BmrFI	CCNGG	4 cut(s)	933, 1042, 1070, 1958
BmsI	GCATC	7 cut(s)	188, 519, 1096, 1486, 1699, 1767, 2233
BpiI	GAAGAC	3 cut(s)	669, 1879, 2223
BpmI	CTGGAG	1 cut(s)	579
Bpu10I	CCTNAGC	2 cut(s)	91, 1284
Bpu14I	TTCGAA	1 cut(s)	1831
BpuEI	CTTGAG	1 cut(s)	182
BpuMI	CCSGG	1 cut(s)	1070
BsaAI	YACGTR	2 cut(s)	2107, 2140
BsaHI	GRCGYC	1 cut(s)	2294
BsaI	GGTCTC	3 cut(s)	24, 1384, 1675
BsaJI	CCNNGG	6 cut(s)	614, 1041, 1083, 1956, 2173, 2230
BsaWI	WCCGGW	1 cut(s)	1520
BsaXI	ACNNNNNCTCC	2 cut(s)	777, 807
Bsc4I	CCNNNNNNNGG	2 cut(s)	683, 2274
Bse118I	RCCGGY	1 cut(s)	969
Bse1I	ACTGG	5 cut(s)	596, 1236, 1273, 2246, 2309
Bse3DI	GCAATG	1 cut(s)	1759
BseBI	CCWGG	3 cut(s)	933, 1042, 1958
BseDI	CCNNGG	6 cut(s)	614, 1041, 1083, 1956, 2173, 2230
BseLI	CCNNNNNNNGG	2 cut(s)	683, 2274
BseMI	GCAATG	1 cut(s)	1759
BseMII	CTCAG	3 cut(s)	105, 1142, 1253
BseNI	ACTGG	5 cut(s)	596, 1236, 1273, 2246, 2309
BseRI	GAGGAG	1 cut(s)	824
BseXI	GCAGC	9 cut(s)	851, 875, 940, 1228, 1231, 1292, 1640, 1792, 2181
BseYI	CCCAGC	1 cut(s)	1226
BshFI	GGCC	2 cut(s)	973, 2178
BshNI	GGYRCC	1 cut(s)	2293
BsiHKAI	GWGCWC	2 cut(s)	92, 880
BsiHKCI	CYCGRG	2 cut(s)	27, 777
BsiSI	CCGG	3 cut(s)	970, 1069, 1521
BslFI	GGGAC	1 cut(s)	704
BslI	CCNNNNNNNGG	2 cut(s)	683, 2274
BsmAI	GTCTC	6 cut(s)	24, 733, 1081, 1384, 1443, 1675
BsmFI	GGGAC	1 cut(s)	704
BsmI	GAATGC	1 cut(s)	64
BsnI	GGCC	2 cut(s)	973, 2178
Bso31I	GGTCTC	3 cut(s)	24, 1384, 1675
BsoBI	CYCGRG	2 cut(s)	27, 777
Bsp119I	TTCGAA	1 cut(s)	1831
Bsp1286I	GDGCHC	3 cut(s)	92, 795, 880
Bsp143I	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
BspACI	CCGC	3 cut(s)	757, 1103, 1924
BspANI	GGCC	2 cut(s)	973, 2178
BspCNI	CTCAG	3 cut(s)	104, 1143, 1254
BspLI	GGNNCC	6 cut(s)	692, 794, 937, 1017, 2024, 2295
BspMAI	CTGCAG	1 cut(s)	2215
BspPI	GGATC	1 cut(s)	859
BspQI	GCTCTTC	3 cut(s)	80, 150, 1101
BspT104I	TTCGAA	1 cut(s)	1831
BspT107I	GGYRCC	1 cut(s)	2293
BspTNI	GGTCTC	3 cut(s)	24, 1384, 1675
BsrDI	GCAATG	1 cut(s)	1759
BsrFI	RCCGGY	1 cut(s)	969
BsrI	ACTGG	5 cut(s)	596, 1236, 1273, 2246, 2309
BssAI	RCCGGY	1 cut(s)	969
BssECI	CCNNGG	6 cut(s)	614, 1041, 1083, 1956, 2173, 2230
BssMI	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
BssNI	GRCGYC	1 cut(s)	2294
BssSI	CACGAG	3 cut(s)	797, 873, 1216
BssT1I	CCWWGG	3 cut(s)	614, 1083, 2173
Bst2BI	CACGAG	3 cut(s)	797, 873, 1216
Bst2UI	CCWGG	3 cut(s)	933, 1042, 1958
Bst4CI	ACNGT	6 cut(s)	464, 484, 571, 1900, 1931, 2160
Bst6I	CTCTTC	7 cut(s)	80, 150, 290, 380, 597, 1101, 1446
BstACI	GRCGYC	1 cut(s)	2294
BstBAI	YACGTR	2 cut(s)	2107, 2140
BstBI	TTCGAA	1 cut(s)	1831
BstC8I	GCNNGC	7 cut(s)	6, 759, 771, 971, 2198, 2211, 2265
BstDEI	CTNAG	6 cut(s)	91, 533, 1151, 1251, 1262, 1284
BstDSI	CCRYGG	1 cut(s)	2230
BstEII	GGTNACC	1 cut(s)	1603
BstH2I	RGCGCY	2 cut(s)	1924, 2297
BstHHI	GCGC	5 cut(s)	381, 1690, 1923, 2257, 2296
BstKTI	GATC	6 cut(s)	302, 337, 854, 1597, 1813, 2284
BstMAI	GTCTC	6 cut(s)	24, 733, 1081, 1384, 1443, 1675
BstMBI	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
BstMWI	GCNNNNNNNGC	7 cut(s)	1100, 1286, 1662, 1780, 1973, 2175, 2206
BstNI	CCWGG	3 cut(s)	933, 1042, 1958
BstNSI	RCATGY	4 cut(s)	890, 1477, 2200, 2224
BstPI	GGTNACC	1 cut(s)	1603
BstSCI	CCNGG	4 cut(s)	931, 1040, 1068, 1956
BstSFI	CTRYAG	1 cut(s)	2211
BstV1I	GCAGC	9 cut(s)	851, 875, 940, 1228, 1231, 1292, 1640, 1792, 2181
BstV2I	GAAGAC	3 cut(s)	669, 1879, 2223
BstX2I	RGATCY	1 cut(s)	334
BstYI	RGATCY	1 cut(s)	334
BsuRI	GGCC	2 cut(s)	973, 2178
BtgI	CCRYGG	1 cut(s)	2230
BtgZI	GCGATG	1 cut(s)	2210
BtsI	GCAGTG	1 cut(s)	1116
BtsIMutI	CAGTG	5 cut(s)	1116, 1243, 1905, 1927, 2253
Cac8I	GCNNGC	7 cut(s)	6, 759, 771, 971, 2198, 2211, 2265
CaiI	CAGNNNCTG	1 cut(s)	845
CfoI	GCGC	5 cut(s)	381, 1690, 1923, 2257, 2296
Cfr10I	RCCGGY	1 cut(s)	969
Cfr13I	GGNCC	5 cut(s)	577, 691, 972, 1556, 1787
CseI	GACGC	2 cut(s)	266, 1982
Csp6I	GTAC	4 cut(s)	211, 480, 1208, 1410
CviAII	CATG	9 cut(s)	439, 887, 1316, 1426, 1474, 1784, 1814, 2197, 2221
CviQI	GTAC	4 cut(s)	211, 480, 1208, 1410
DdeI	CTNAG	6 cut(s)	91, 533, 1151, 1251, 1262, 1284
DinI	GGCGCC	1 cut(s)	2295
DpnI	GATC	6 cut(s)	301, 336, 853, 1596, 1812, 2283
DpnII	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
DraI	TTTAAA	2 cut(s)	1990, 2121
Eam1104I	CTCTTC	7 cut(s)	80, 150, 290, 380, 597, 1101, 1446
EarI	CTCTTC	7 cut(s)	80, 150, 290, 380, 597, 1101, 1446
Ecl136II	GAGCTC	1 cut(s)	90
Eco130I	CCWWGG	3 cut(s)	614, 1083, 2173
Eco24I	GRGCYC	2 cut(s)	92, 795
Eco31I	GGTCTC	3 cut(s)	24, 1384, 1675
Eco47I	GGWCC	4 cut(s)	577, 691, 1556, 1787
Eco53kI	GAGCTC	1 cut(s)	90
Eco57I	CTGAAG	3 cut(s)	561, 1347, 2031
Eco72I	CACGTG	2 cut(s)	2107, 2140
Eco88I	CYCGRG	2 cut(s)	27, 777
Eco91I	GGTNACC	1 cut(s)	1603
EcoICRI	GAGCTC	1 cut(s)	90
EcoO65I	GGTNACC	1 cut(s)	1603
EcoRII	CCWGG	3 cut(s)	931, 1040, 1956
EcoT14I	CCWWGG	3 cut(s)	614, 1083, 2173
EcoT22I	ATGCAT	2 cut(s)	1479, 2226
EcoT38I	GRGCYC	2 cut(s)	92, 795
EgeI	GGCGCC	1 cut(s)	2295
EheI	GGCGCC	1 cut(s)	2295
ErhI	CCWWGG	3 cut(s)	614, 1083, 2173
FaeI	CATG	9 cut(s)	442, 890, 1319, 1429, 1477, 1787, 1817, 2200, 2224
FaqI	GGGAC	1 cut(s)	704
FatI	CATG	9 cut(s)	438, 886, 1315, 1425, 1473, 1783, 1813, 2196, 2220
FauI	CCCGC	1 cut(s)	750
FbaI	TGATCA	2 cut(s)	1810, 2281
FriOI	GRGCYC	2 cut(s)	92, 795
FspBI	CTAG	9 cut(s)	477, 615, 836, 966, 1100, 1116, 1185, 1481, 2201
GlaI	GCGC	5 cut(s)	380, 1689, 1922, 2256, 2295
GsaI	CCCAGC	1 cut(s)	1230
GsuI	CTGGAG	1 cut(s)	579
HaeII	RGCGCY	2 cut(s)	1924, 2297
HaeIII	GGCC	2 cut(s)	973, 2178
HapII	CCGG	3 cut(s)	970, 1069, 1521
HgaI	GACGC	2 cut(s)	266, 1982
HhaI	GCGC	5 cut(s)	381, 1690, 1923, 2257, 2296
Hin1I	GRCGYC	1 cut(s)	2294
Hin1II	CATG	9 cut(s)	442, 890, 1319, 1429, 1477, 1787, 1817, 2200, 2224
Hin6I	GCGC	5 cut(s)	379, 1688, 1921, 2255, 2294
HinP1I	GCGC	5 cut(s)	379, 1688, 1921, 2255, 2294
HincII	GTYRAC	1 cut(s)	1198
HindII	GTYRAC	1 cut(s)	1198
HindIII	AAGCTT	1 cut(s)	1144
HinfI	GANTC	4 cut(s)	12, 1088, 1181, 1189
HpaI	GTTAAC	1 cut(s)	1198
HpaII	CCGG	3 cut(s)	970, 1069, 1521
HphI	GGTGA	5 cut(s)	210, 496, 925, 1268, 1481
Hpy166II	GTNNAC	6 cut(s)	833, 1157, 1198, 1259, 2137, 2234
Hpy188III	TCNNGA	9 cut(s)	29, 132, 234, 659, 779, 1150, 1805, 1840, 2285
Hpy8I	GTNNAC	6 cut(s)	833, 1157, 1198, 1259, 2137, 2234
HpyCH4III	ACNGT	6 cut(s)	464, 484, 571, 1900, 1931, 2160
HpyCH4IV	ACGT	2 cut(s)	2106, 2139
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1100, 1286, 1662, 1780, 1973, 2175, 2206
HpyF3I	CTNAG	6 cut(s)	91, 533, 1151, 1251, 1262, 1284
HpySE526I	ACGT	2 cut(s)	2106, 2139
Hsp92I	GRCGYC	1 cut(s)	2294
Hsp92II	CATG	9 cut(s)	442, 890, 1319, 1429, 1477, 1787, 1817, 2200, 2224
HspAI	GCGC	5 cut(s)	379, 1688, 1921, 2255, 2294
KasI	GGCGCC	1 cut(s)	2293
KroI	GCCGGC	1 cut(s)	969
KroNI	GCCGGC	1 cut(s)	971
Ksp22I	TGATCA	2 cut(s)	1810, 2281
KspAI	GTTAAC	1 cut(s)	1198
Kzo9I	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
LguI	GCTCTTC	3 cut(s)	80, 150, 1101
LmnI	GCTCC	6 cut(s)	117, 798, 811, 1015, 1981, 2022
Lsp1109I	GCAGC	9 cut(s)	851, 875, 940, 1228, 1231, 1292, 1640, 1792, 2181
LweI	GCATC	7 cut(s)	188, 519, 1096, 1486, 1699, 1767, 2233
MaeI	CTAG	9 cut(s)	477, 615, 836, 966, 1100, 1116, 1185, 1481, 2201
MaeII	ACGT	2 cut(s)	2106, 2139
MaeIII	GTNAC	2 cut(s)	1469, 1603
MalI	GATC	6 cut(s)	301, 336, 853, 1596, 1812, 2283
MboI	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
MfeI	CAATTG	2 cut(s)	1704, 1998
MflI	RGATCY	1 cut(s)	334
MhlI	GDGCHC	3 cut(s)	92, 795, 880
Mly113I	GGCGCC	1 cut(s)	2294
MlyI	GAGTC	2 cut(s)	6, 1082
MmeI	TCCRAC	4 cut(s)	501, 716, 963, 1021
Mph1103I	ATGCAT	2 cut(s)	1479, 2226
MroNI	GCCGGC	1 cut(s)	969
MroXI	GAANNNNTTC	4 cut(s)	1161, 1332, 1714, 1827
MseI	TTAA	7 cut(s)	623, 636, 744, 1197, 1989, 2120, 2271
MslI	CAYNNNNRTG	2 cut(s)	885, 2229
MspI	CCGG	3 cut(s)	970, 1069, 1521
MspR9I	CCNGG	4 cut(s)	933, 1042, 1070, 1958
MunI	CAATTG	2 cut(s)	1704, 1998
Mva1269I	GAATGC	1 cut(s)	64
MvaI	CCWGG	3 cut(s)	933, 1042, 1958
MwoI	GCNNNNNNNGC	7 cut(s)	1100, 1286, 1662, 1780, 1973, 2175, 2206
NaeI	GCCGGC	1 cut(s)	971
NarI	GGCGCC	1 cut(s)	2294
NciI	CCSGG	1 cut(s)	1070
NdeII	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
NgoMIV	GCCGGC	1 cut(s)	969
NlaIII	CATG	9 cut(s)	442, 890, 1319, 1429, 1477, 1787, 1817, 2200, 2224
NlaIV	GGNNCC	6 cut(s)	692, 794, 937, 1017, 2024, 2295
NmuCI	GTSAC	1 cut(s)	1469
NsiI	ATGCAT	2 cut(s)	1479, 2226
NspI	RCATGY	4 cut(s)	890, 1477, 2200, 2224
NspV	TTCGAA	1 cut(s)	1831
PaeI	GCATGC	1 cut(s)	2200
PaeR7I	CTCGAG	2 cut(s)	27, 777
PciSI	GCTCTTC	3 cut(s)	80, 150, 1101
PcsI	WCGNNNNNNNCGW	1 cut(s)	2233
PctI	GAATGC	1 cut(s)	64
PdiI	GCCGGC	1 cut(s)	971
PdmI	GAANNNNTTC	4 cut(s)	1161, 1332, 1714, 1827
PfeI	GAWTC	2 cut(s)	1181, 1189
PleI	GAGTC	2 cut(s)	6, 1082
PluTI	GGCGCC	1 cut(s)	2297
PmaCI	CACGTG	2 cut(s)	2107, 2140
PmlI	CACGTG	2 cut(s)	2107, 2140
PpsI	GAGTC	2 cut(s)	6, 1082
Ppu21I	YACGTR	2 cut(s)	2107, 2140
PsiI	TTATAA	1 cut(s)	2189
Psp124BI	GAGCTC	1 cut(s)	92
Psp6I	CCWGG	3 cut(s)	931, 1040, 1956
PspCI	CACGTG	2 cut(s)	2107, 2140
PspEI	GGTNACC	1 cut(s)	1603
PspFI	CCCAGC	1 cut(s)	1226
PspGI	CCWGG	3 cut(s)	931, 1040, 1956
PspN4I	GGNNCC	6 cut(s)	692, 794, 937, 1017, 2024, 2295
PspPI	GGNCC	5 cut(s)	577, 691, 972, 1556, 1787
PstI	CTGCAG	1 cut(s)	2215
PstNI	CAGNNNCTG	1 cut(s)	845
PsuI	RGATCY	1 cut(s)	334
RsaI	GTAC	4 cut(s)	212, 481, 1209, 1411
RsaNI	GTAC	4 cut(s)	211, 480, 1208, 1410
RseI	CAYNNNNRTG	2 cut(s)	885, 2229
SacI	GAGCTC	1 cut(s)	92
SapI	GCTCTTC	3 cut(s)	80, 150, 1101
SaqAI	TTAA	7 cut(s)	623, 636, 744, 1197, 1989, 2120, 2271
Sau3AI	GATC	6 cut(s)	299, 334, 851, 1594, 1810, 2281
Sau96I	GGNCC	5 cut(s)	577, 691, 972, 1556, 1787
ScaI	AGTACT	1 cut(s)	212
SchI	GAGTC	2 cut(s)	6, 1082
ScrFI	CCNGG	4 cut(s)	933, 1042, 1070, 1958
SduI	GDGCHC	3 cut(s)	92, 795, 880
SfaNI	GCATC	7 cut(s)	188, 519, 1096, 1486, 1699, 1767, 2233
SfcI	CTRYAG	1 cut(s)	2211
SfoI	GGCGCC	1 cut(s)	2295
Sfr274I	CTCGAG	2 cut(s)	27, 777
SfuI	TTCGAA	1 cut(s)	1831
SinI	GGWCC	4 cut(s)	577, 691, 1556, 1787
SlaI	CTCGAG	2 cut(s)	27, 777
SmiMI	CAYNNNNRTG	2 cut(s)	885, 2229
SmlI	CTYRAG	4 cut(s)	14, 27, 161, 777
SmoI	CTYRAG	4 cut(s)	14, 27, 161, 777
SpeI	ACTAGT	1 cut(s)	476
SphI	GCATGC	1 cut(s)	2200
SsiI	CCGC	3 cut(s)	757, 1103, 1924
SspDI	GGCGCC	1 cut(s)	2293
SspI	AATATT	1 cut(s)	455
SspMI	CTAG	9 cut(s)	477, 615, 836, 966, 1100, 1116, 1185, 1481, 2201
SstI	GAGCTC	1 cut(s)	92
StyD4I	CCNGG	4 cut(s)	931, 1040, 1068, 1956
StyI	CCWWGG	3 cut(s)	614, 1083, 2173
TaaI	ACNGT	6 cut(s)	464, 484, 571, 1900, 1931, 2160
TaiI	ACGT	2 cut(s)	2109, 2142
TaqI	TCGA	5 cut(s)	28, 302, 573, 778, 1831
TaqII	GACCGA	2 cut(s)	276, 708
TatI	WGTACW	2 cut(s)	210, 1207
TauI	GCSGC	1 cut(s)	1926
TfiI	GAWTC	2 cut(s)	1181, 1189
Tru1I	TTAA	7 cut(s)	623, 636, 744, 1197, 1989, 2120, 2271
Tru9I	TTAA	7 cut(s)	623, 636, 744, 1197, 1989, 2120, 2271
TscAI	CASTG	5 cut(s)	1116, 1243, 1905, 1934, 2253
TseFI	GTSAC	1 cut(s)	1469
TseI	GCWGC	9 cut(s)	839, 863, 928, 1241, 1244, 1280, 1653, 1780, 2169
Tsp45I	GTSAC	1 cut(s)	1469
TspRI	CASTG	5 cut(s)	1116, 1243, 1905, 1934, 2253
VpaK11BI	GGWCC	4 cut(s)	577, 691, 1556, 1787
XapI	RAATTY	3 cut(s)	52, 1827, 1834
XceI	RCATGY	4 cut(s)	890, 1477, 2200, 2224
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2111
XhoI	CTCGAG	2 cut(s)	27, 777
XmaJI	CCTAGG	1 cut(s)	614
XmnI	GAANNNNTTC	4 cut(s)	1161, 1332, 1714, 1827
XspI	CTAG	9 cut(s)	477, 615, 836, 966, 1100, 1116, 1185, 1481, 2201
ZrmI	AGTACT	1 cut(s)	212
Zsp2I	ATGCAT	2 cut(s)	1479, 2226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.