Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002851

Physical Location & Seq

Genomic Coordinates Forward (+)

19826729 .. 19834992

Gene Structure 8264 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1209600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2412 bp

ATGCTTGCTGGAGTGAAGATCATACCCCGAGACCAGATTGATAAGGATGAGAATCTGAGTGCTCCTGGGAAAAAAAAACAAAAACAGAAGAAGAGGTCGGGTAATAAGGAGAATAAGTGCAGGAAGGAAAAGAAGCGCTCTCAATCTAGTAGTTCGGATGGGGAAGAGCTTGATAGAATAAAGAAGGGGTCTAAAAAAAAATGGTATTCGTCAGACAAGTATTCATCATCATCATCATATTCGTCAGGCGTTGAAAGTGAGGGAAGTTCTGACCAAGATAGAAGGAGGAGTCAGAGTAGGAAAAAAGGTAAGAGAAAAAGAAGTAATGAGGATGATTCAGAGGATGAGGCCAGTTATAGGTCAAAAAGGAGATCAAGGAGGGGAAGGAAAGAATATGTATCAGAAGATCATTATTCTTCTGCTTCTGATGGTAAAGACAGTGATAGTTGTTCTGATAGAAAAGAAAGGTGCAAAAACGGAAGTAAAAAAGACAGAAAGAAAAAGAGTAAGCGAAGGAGTCTAGGAGGCATGGGGGATGAAGAATTTACTGATGGAGATAGAGGACCCAATCGTTTGAAAGATGGGGAGATTGCAAGGAAGGAATTTGGCTTAGAGTGGATGCTTAGACCTGAAGGAAAAACAGATAAAACCCCTACAGTGAATGTTGAGGAGCAGCCAAAGGAAAATCCAACTGAAGAGATAAAGGTCAATCCCAGGGAATTAAATCCATATTTAAAGGATGACGGATCTGGTTATCCAGAAGATACAGATGAACCTAAAGCTGCTGGAAATCAACTTTTGTCTTCCTCAGTTGTTGGGGATGGAGGTGCAAGTTGGAAACTAAAAGCTCTGAAGCGTGCAAAAGAGCAGGCGACTCGAGAAGGACGAAGGCTCCAGGAGGTTATCGGAGAGCGTTGGGGTTCTCTTGGTCAACTGACCGTCTCTGTGGCATCTAATAAAGCTGCCCAATCTCGTGCTCATCTACAGGCCATAAGAAGTAGACAGAAAGGGCTGAGTGAGGAACGCCAACCAGATTCCAGCAATCAAAGTGACAAGGATAATAAAAAGGACACTGGTCGGCACTACCTGAAGGATATATCTTGTGGACTCCCTAAAATGAGGGAGCCTAAAGTTCAAGATAAATTTTCCTGGATAAGGCAAGACGTTTCTACTCAGGATTCTAGTCTTTTAGCGGATGCAGTGGCTAGCTTAAATAAGTTTTCAAACGATGGAAGCTTCATGTCTGAAGTTCTTCGTAAAAGGACTGATGATTTTAGTAGTCATGCTCATGAAAATATAGCAGCCACTAGGAAACCAAGTGAAGCGAGTGCTGCATTTCATGATCTAAGTGCGAACCAGTTGGCAGCTAAGGCTTTTCAACTTCGTATGAAAGGAAAGCTAGAAGAAGCTGAGGAACTTCTGCAAGAAGTAGAAAACAGAAAGGCAAAGCAGGCTAGTGGAGATAATTCTGTGAGACCAGGAAATGAACAGAGCACAAGCAGACATGTTATGCAGGAAATGAATCTTCGTCAGAAGAAAAAGGAGGATGATGCTGATATGCATCTAGCTCAAAGGATTACCCAGAACAAAAAATACAGTATATCTGGTCAGGCAGATGATGAATATGACTATGATGATGCTCCAAGAAAGAAGTCCAAGAAAAAGCAGGGTTGTGATAAAAAAGTCACCCAGAACAACAGTTTTGCAACTCGATTTGCAACGCAGCAGGAGAGATGTCTCTTTTGTTTTGAAAATCCGAGGAGGCCGGCGCATCTTGTTGTGGCCATAGCAAACTTCACATATTTAATGTTGCCACAGCAGCAGCCTGTTGTGCCTGGTCACTGTTGTATTTTACCAATGCAGCATGTGGCTTCCACAAGAACTGTTGATGACGACATGTGGCAAGAAATTCGAAATTTCAAGAAATGCCTTATTATGATGTTTGCAAAGCAAGAGAAGGAAGTAGTTTTTCTTGAAACTGTGATGGGGTTGGCTCAACAACGCCGCCACTGTATAGTTGAGTGTATTCCTATGCCCCATGAAATTGCCAAAGAGGCTCCTTTATATTTCAAAAAGGCAATTGATGAAGCTGAAGACGAGTGGAGCCAGCACAATGCGAAGAGACTAATCGATACAAGCGTGAAGGGCTTGCGTGGGTCGATTCCAAAGGACTTTCCGTACTTCCATGTGGAGTTCGGATTGAATAAAGGGTTTGTCCACGTTATTGATGACGAACAGCAGTTCAACAGCAGTCTTGGGCTGAATGTCATTAGAGGCATGCTACAATTGCCTGAGGAAGACATGTATCGTCGCCGAAGACATGAGACGGTGGAGGCACAAAAGCAAGCGGTTAAGAGCTTCGATCAAGATTGGGCAGCTTTTGACTGGACAAAACAGCTTCACCAATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CwfJ_C_1 CwfJ_C_2

Protein Analysis

804

Amino Acids

91.64

Weight (kDa)

9.26

Isoelectric Point (pI)

61.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CwfJ_C_1	PF04677	571 - 691	3.8e-42	Protein similar to CwfJ C-terminal
CwfJ_C_2	PF04676	700 - 797	3.1e-28	Protein similar to CwfJ C-terminus 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56290
fragaria_vesca	FvH4_4g02820 FvH4_4g02820 FvH4_4g02820 FvH4_4g02820
malus_domestica	MD13G1208100.v1.1 MD16G1209600.v1.1
prunus_persica	Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1
pyrus_communis	pycom13g18020 pycom16g17690
rosa_chinensis	RchiOBHm_Chr4g0390791 RchiOBHm_Chr5g0000461
rosa_laevigata	RLG00000009911 RLG00000030869
rosa_multiflora	Rmu_sc0000171.1_g000026 Rmu_sc0001380.1_g000027 Rmu_sc0003949.1_g000006
rosa_roxburghii	Rroxscaffold_1G00075700 Rroxscaffold_5G00336660 Rroxscaffold_5G00336680 Rroxscaffold_5G00336690
rosa_rugosa	Rorug03G0331500 Rorug03G0331500 Rorug04G0383800
rosa_samantha	Rh4AG033200 Rh4BG027000 Rh4CG036800 Rh4DG030000 Rh5AG003800 Rh5BG005000 Rh5CG004100 Rh5DG004000
rosa_wichuraiana	Rw5G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1000
AciI	CCGC	3 cut(s)	1193, 2005, 2348
AclWI	GGATC	1 cut(s)	754
AcoI	YGGCCR	1 cut(s)	1782
AcsI	RAATTY	5 cut(s)	542, 602, 1142, 1908, 1915
AcuI	CTGAAG	6 cut(s)	651, 714, 872, 1109, 1266, 2112
AfaI	GTAC	1 cut(s)	2180
AfeI	AGCGCT	1 cut(s)	137
AfiI	CCNNNNNNNGG	2 cut(s)	357, 1155
AflIII	ACRYGT	3 cut(s)	1504, 1896, 2301
AjnI	CCWGG	6 cut(s)	64, 713, 894, 1148, 1477, 1834
Alw21I	GWGCWC	3 cut(s)	64, 979, 1496
Alw26I	GTCTC	6 cut(s)	24, 946, 1468, 1742, 2116, 2318
AlwI	GGATC	1 cut(s)	754
Ama87I	CYCGRG	2 cut(s)	27, 876
Aor51HI	AGCGCT	1 cut(s)	137
AoxI	GGCC	4 cut(s)	348, 987, 1763, 1782
ApoI	RAATTY	5 cut(s)	542, 602, 1142, 1908, 1915
Asp700I	GAANNNNTTC	1 cut(s)	1251
AspLEI	GCGC	2 cut(s)	138, 1771
AspS9I	GGNCC	1 cut(s)	563
AsuHPI	GGTGA	2 cut(s)	1678, 2393
AsuII	TTCGAA	1 cut(s)	1912
AsuNHI	GCTAGC	1 cut(s)	1205
AvaI	CYCGRG	2 cut(s)	27, 876
AvaII	GGWCC	1 cut(s)	563
AxyI	CCTNAGG	1 cut(s)	2292
BaeI	ACNNNNGTAYC	2 cut(s)	2124, 2157
BalI	TGGCCA	1 cut(s)	1784
BauI	CACGAG	1 cut(s)	972
BbsI	GAAGAC	4 cut(s)	795, 2100, 2304, 2323
Bbv12I	GWGCWC	3 cut(s)	64, 979, 1496
BbvCI	CCTCAGC	1 cut(s)	1410
BccI	CCATC	7 cut(s)	152, 422, 545, 575, 815, 1223, 1978
BcgI	CGANNNNNNTGC	4 cut(s)	857, 891, 1892, 1926
BciT130I	CCWGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
BcoDI	GTCTC	6 cut(s)	24, 946, 1468, 1742, 2116, 2318
BfaI	CTAG	8 cut(s)	147, 521, 1182, 1206, 1308, 1400, 1455, 1565
BfmI	CTRYAG	2 cut(s)	654, 983
BfoI	RGCGCY	1 cut(s)	139
BglI	GCCNNNNNGGC	1 cut(s)	2054
Bme1390I	CCNGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
Bme18I	GGWCC	1 cut(s)	563
BmeT110I	CYCGRG	2 cut(s)	27, 876
BmgT120I	GGNCC	1 cut(s)	563
BmiI	GGNNCC	5 cut(s)	565, 893, 1125, 2058, 2105
BmrFI	CCNGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
BmsI	GCATC	7 cut(s)	609, 959, 1186, 1540, 1570, 1627, 1780
BmtI	GCTAGC	1 cut(s)	1209
BoxI	GACNNNNGTC	1 cut(s)	1074
BpiI	GAAGAC	4 cut(s)	795, 2100, 2304, 2323
BpmI	CTGGAG	2 cut(s)	30, 878
Bpu10I	CCTNAGC	2 cut(s)	1368, 1410
Bpu14I	TTCGAA	1 cut(s)	1912
Bsa29I	ATCGAT	1 cut(s)	2130
BsaBI	GATNNNNATC	2 cut(s)	51, 1560
BsaI	GGTCTC	2 cut(s)	24, 1468
BsaJI	CCNNGG	4 cut(s)	65, 713, 714, 1757
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 280, 310, 876, 906
Bsc4I	CCNNNNNNNGG	2 cut(s)	357, 1155
Bse118I	RCCGGY	1 cut(s)	1765
Bse1I	ACTGG	4 cut(s)	351, 1078, 1357, 2390
Bse21I	CCTNAGG	1 cut(s)	2292
Bse8I	GATNNNNATC	2 cut(s)	51, 1560
BseBI	CCWGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
BseCI	ATCGAT	1 cut(s)	2130
BseDI	CCNNGG	4 cut(s)	65, 713, 714, 1757
BseJI	GATNNNNATC	2 cut(s)	51, 1560
BseLI	CCNNNNNNNGG	2 cut(s)	357, 1155
BseMII	CTCAG	6 cut(s)	47, 822, 1004, 1187, 1401, 2283
BseNI	ACTGG	4 cut(s)	351, 1078, 1357, 2390
BseRI	GAGGAG	3 cut(s)	301, 683, 1774
BsgI	GTGCAG	1 cut(s)	139
BshFI	GGCC	4 cut(s)	350, 989, 1765, 1784
BshVI	ATCGAT	1 cut(s)	2130
BsiHKAI	GWGCWC	3 cut(s)	64, 979, 1496
BsiHKCI	CYCGRG	2 cut(s)	27, 876
BsiSI	CCGG	1 cut(s)	1766
BslI	CCNNNNNNNGG	2 cut(s)	357, 1155
BsmAI	GTCTC	6 cut(s)	24, 946, 1468, 1742, 2116, 2318
BsmBI	CGTCTC	2 cut(s)	946, 2318
BsnI	GGCC	4 cut(s)	350, 989, 1765, 1784
Bso31I	GGTCTC	2 cut(s)	24, 1468
BsoBI	CYCGRG	2 cut(s)	27, 876
Bsp119I	TTCGAA	1 cut(s)	1912
Bsp1286I	GDGCHC	3 cut(s)	64, 979, 1496
Bsp143I	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
BspACI	CCGC	3 cut(s)	1193, 2005, 2348
BspANI	GGCC	4 cut(s)	350, 989, 1765, 1784
BspCNI	CTCAG	6 cut(s)	48, 821, 1005, 1186, 1402, 2284
BspDI	ATCGAT	1 cut(s)	2130
BspHI	TCATGA	2 cut(s)	1288, 1339
BspLI	GGNNCC	5 cut(s)	565, 893, 1125, 2058, 2105
BspOI	GCTAGC	1 cut(s)	1209
BspPI	GGATC	1 cut(s)	754
BspQI	GCTCTTC	1 cut(s)	159
BspT104I	TTCGAA	1 cut(s)	1912
BspTNI	GGTCTC	2 cut(s)	24, 1468
BsrFI	RCCGGY	1 cut(s)	1765
BsrI	ACTGG	4 cut(s)	351, 1078, 1357, 2390
BssAI	RCCGGY	1 cut(s)	1765
BssECI	CCNNGG	4 cut(s)	65, 713, 714, 1757
BssMI	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
BssSI	CACGAG	1 cut(s)	972
Bst2BI	CACGAG	1 cut(s)	972
Bst2UI	CCWGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
Bst6I	CTCTTC	4 cut(s)	86, 159, 690, 2114
BstBI	TTCGAA	1 cut(s)	1912
BstENI	CCTNNNNNAGG	1 cut(s)	1153
BstH2I	RGCGCY	1 cut(s)	139
BstHHI	GCGC	2 cut(s)	138, 1771
BstKTI	GATC	6 cut(s)	21, 374, 409, 749, 1345, 2365
BstMAI	GTCTC	6 cut(s)	24, 946, 1468, 1742, 2116, 2318
BstMBI	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
BstMWI	GCNNNNNNNGC	8 cut(s)	1331, 1370, 1451, 1819, 1831, 2054, 2145, 2287
BstNI	CCWGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
BstNSI	RCATGY	5 cut(s)	1508, 1868, 1900, 2281, 2305
BstPAI	GACNNNNGTC	1 cut(s)	1074
BstSCI	CCNGG	6 cut(s)	64, 713, 894, 1148, 1477, 1834
BstSFI	CTRYAG	2 cut(s)	654, 983
BstV2I	GAAGAC	4 cut(s)	795, 2100, 2304, 2323
BstX2I	RGATCY	1 cut(s)	746
BstYI	RGATCY	1 cut(s)	746
Bsu15I	ATCGAT	1 cut(s)	2130
Bsu36I	CCTNAGG	1 cut(s)	2292
BsuRI	GGCC	4 cut(s)	350, 989, 1765, 1784
BsuTUI	ATCGAT	1 cut(s)	2130
BtsI	GCAGTG	1 cut(s)	1206
BtsIMutI	CAGTG	6 cut(s)	445, 663, 1071, 1206, 1840, 2008
CciI	TCATGA	2 cut(s)	1288, 1339
CfoI	GCGC	2 cut(s)	138, 1771
Cfr10I	RCCGGY	1 cut(s)	1765
Cfr13I	GGNCC	1 cut(s)	563
ClaI	ATCGAT	1 cut(s)	2130
Csp6I	GTAC	1 cut(s)	2179
CviQI	GTAC	1 cut(s)	2179
DpnI	GATC	6 cut(s)	20, 373, 408, 748, 1344, 2364
DpnII	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
DraI	TTTAAA	1 cut(s)	735
EaeI	YGGCCR	1 cut(s)	1782
Eam1104I	CTCTTC	4 cut(s)	86, 159, 690, 2114
EarI	CTCTTC	4 cut(s)	86, 159, 690, 2114
Eco31I	GGTCTC	2 cut(s)	24, 1468
Eco47I	GGWCC	1 cut(s)	563
Eco47III	AGCGCT	1 cut(s)	137
Eco57I	CTGAAG	6 cut(s)	651, 714, 872, 1109, 1266, 2112
Eco81I	CCTNAGG	1 cut(s)	2292
Eco88I	CYCGRG	2 cut(s)	27, 876
EcoNI	CCTNNNNNAGG	1 cut(s)	1153
EcoO109I	RGGNCCY	1 cut(s)	563
EcoRII	CCWGG	6 cut(s)	64, 713, 894, 1148, 1477, 1834
EcoT22I	ATGCAT	1 cut(s)	1563
Esp3I	CGTCTC	2 cut(s)	946, 2318
FblI	GTMKAC	1 cut(s)	1000
FspBI	CTAG	8 cut(s)	147, 521, 1182, 1206, 1308, 1400, 1455, 1565
GlaI	GCGC	2 cut(s)	137, 1770
GsuI	CTGGAG	2 cut(s)	30, 878
HaeII	RGCGCY	1 cut(s)	139
HaeIII	GGCC	4 cut(s)	350, 989, 1765, 1784
HapII	CCGG	1 cut(s)	1766
HhaI	GCGC	2 cut(s)	138, 1771
Hin6I	GCGC	2 cut(s)	136, 1769
HinP1I	GCGC	2 cut(s)	136, 1769
HincII	GTYRAC	1 cut(s)	932
HindII	GTYRAC	1 cut(s)	932
HindIII	AAGCTT	1 cut(s)	1234
HpaII	CCGG	1 cut(s)	1766
HphI	GGTGA	2 cut(s)	1678, 2393
Hpy166II	GTNNAC	4 cut(s)	932, 1001, 1106, 2218
Hpy188III	TCNNGA	9 cut(s)	758, 878, 1136, 1175, 1289, 1340, 1921, 1973, 2366
Hpy8I	GTNNAC	4 cut(s)	932, 1001, 1106, 2218
Hpy99I	CGWCG	1 cut(s)	2313
HpyCH4IV	ACGT	2 cut(s)	1164, 2220
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1331, 1370, 1451, 1819, 1831, 2054, 2145, 2287
HpySE526I	ACGT	2 cut(s)	1164, 2220
HspAI	GCGC	2 cut(s)	136, 1769
KroI	GCCGGC	1 cut(s)	1765
KroNI	GCCGGC	1 cut(s)	1767
Kzo9I	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
LguI	GCTCTTC	1 cut(s)	159
LmnI	GCTCC	7 cut(s)	67, 670, 897, 1123, 1645, 2062, 2103
LweI	GCATC	7 cut(s)	609, 959, 1186, 1540, 1570, 1627, 1780
MaeI	CTAG	8 cut(s)	147, 521, 1182, 1206, 1308, 1400, 1455, 1565
MaeII	ACGT	2 cut(s)	1164, 2220
MaeIII	GTNAC	3 cut(s)	1049, 1684, 1838
MalI	GATC	6 cut(s)	20, 373, 408, 748, 1344, 2364
MboI	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
MfeI	CAATTG	2 cut(s)	2079, 2285
MflI	RGATCY	1 cut(s)	746
MhlI	GDGCHC	3 cut(s)	64, 979, 1496
MlsI	TGGCCA	1 cut(s)	1784
MluNI	TGGCCA	1 cut(s)	1784
MlyI	GAGTC	4 cut(s)	298, 526, 868, 1101
MmeI	TCCRAC	2 cut(s)	713, 815
Mox20I	TGGCCA	1 cut(s)	1784
Mph1103I	ATGCAT	1 cut(s)	1563
MroNI	GCCGGC	1 cut(s)	1765
MroXI	GAANNNNTTC	1 cut(s)	1251
MscI	TGGCCA	1 cut(s)	1784
MseI	TTAA	5 cut(s)	722, 734, 1211, 1805, 2352
MslI	CAYNNNNRTG	1 cut(s)	1287
Msp20I	TGGCCA	1 cut(s)	1784
MspI	CCGG	1 cut(s)	1766
MspR9I	CCNGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
MunI	CAATTG	2 cut(s)	2079, 2285
MvaI	CCWGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
MwoI	GCNNNNNNNGC	8 cut(s)	1331, 1370, 1451, 1819, 1831, 2054, 2145, 2287
NaeI	GCCGGC	1 cut(s)	1767
NdeII	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
NgoMIV	GCCGGC	1 cut(s)	1765
NheI	GCTAGC	1 cut(s)	1205
NlaIV	GGNNCC	5 cut(s)	565, 893, 1125, 2058, 2105
NmuCI	GTSAC	3 cut(s)	1049, 1684, 1838
NsiI	ATGCAT	1 cut(s)	1563
NspI	RCATGY	5 cut(s)	1508, 1868, 1900, 2281, 2305
NspV	TTCGAA	1 cut(s)	1912
PaeI	GCATGC	1 cut(s)	2281
PaeR7I	CTCGAG	1 cut(s)	876
PagI	TCATGA	2 cut(s)	1288, 1339
PasI	CCCWGGG	1 cut(s)	714
PciI	ACATGT	3 cut(s)	1504, 1896, 2301
PciSI	GCTCTTC	1 cut(s)	159
PcsI	WCGNNNNNNNCGW	2 cut(s)	883, 2136
PdiI	GCCGGC	1 cut(s)	1767
PdmI	GAANNNNTTC	1 cut(s)	1251
PfeI	GAWTC	6 cut(s)	52, 335, 1034, 1178, 1522, 2161
PfoI	TCCNGGA	2 cut(s)	894, 1148
PleI	GAGTC	4 cut(s)	297, 525, 868, 1101
PpsI	GAGTC	4 cut(s)	297, 525, 868, 1101
PpuMI	RGGWCCY	1 cut(s)	563
PscI	ACATGT	3 cut(s)	1504, 1896, 2301
PshAI	GACNNNNGTC	1 cut(s)	1074
Psp5II	RGGWCCY	1 cut(s)	563
Psp6I	CCWGG	6 cut(s)	64, 713, 894, 1148, 1477, 1834
PspGI	CCWGG	6 cut(s)	64, 713, 894, 1148, 1477, 1834
PspN4I	GGNNCC	5 cut(s)	565, 893, 1125, 2058, 2105
PspPI	GGNCC	1 cut(s)	563
PspPPI	RGGWCCY	1 cut(s)	563
PsrI	GAACNNNNNNTAC	2 cut(s)	1577, 1609
PsuI	RGATCY	1 cut(s)	746
RsaI	GTAC	1 cut(s)	2180
RsaNI	GTAC	1 cut(s)	2179
RseI	CAYNNNNRTG	1 cut(s)	1287
SapI	GCTCTTC	1 cut(s)	159
SaqAI	TTAA	5 cut(s)	722, 734, 1211, 1805, 2352
Sau3AI	GATC	6 cut(s)	18, 371, 406, 746, 1342, 2362
Sau96I	GGNCC	1 cut(s)	563
SchI	GAGTC	4 cut(s)	298, 526, 868, 1101
ScrFI	CCNGG	6 cut(s)	66, 715, 896, 1150, 1479, 1836
SduI	GDGCHC	3 cut(s)	64, 979, 1496
SfaNI	GCATC	7 cut(s)	609, 959, 1186, 1540, 1570, 1627, 1780
SfcI	CTRYAG	2 cut(s)	654, 983
Sfr274I	CTCGAG	1 cut(s)	876
SfuI	TTCGAA	1 cut(s)	1912
SinI	GGWCC	1 cut(s)	563
SlaI	CTCGAG	1 cut(s)	876
SmiMI	CAYNNNNRTG	1 cut(s)	1287
SmlI	CTYRAG	1 cut(s)	876
SmoI	CTYRAG	1 cut(s)	876
SphI	GCATGC	1 cut(s)	2281
SsiI	CCGC	3 cut(s)	1193, 2005, 2348
SspMI	CTAG	8 cut(s)	147, 521, 1182, 1206, 1308, 1400, 1455, 1565
StyD4I	CCNGG	6 cut(s)	64, 713, 894, 1148, 1477, 1834
TaiI	ACGT	2 cut(s)	1167, 2223
TaqI	TCGA	6 cut(s)	877, 1711, 1912, 2130, 2159, 2361
TauI	GCSGC	1 cut(s)	2007
TfiI	GAWTC	6 cut(s)	52, 335, 1034, 1178, 1522, 2161
Tru1I	TTAA	5 cut(s)	722, 734, 1211, 1805, 2352
Tru9I	TTAA	5 cut(s)	722, 734, 1211, 1805, 2352
TscAI	CASTG	6 cut(s)	445, 663, 1078, 1206, 1847, 2015
TseFI	GTSAC	3 cut(s)	1049, 1684, 1838
Tsp45I	GTSAC	3 cut(s)	1049, 1684, 1838
TspGWI	ACGGA	3 cut(s)	492, 759, 2166
TspRI	CASTG	6 cut(s)	445, 663, 1078, 1206, 1847, 2015
VpaK11BI	GGWCC	1 cut(s)	563
XagI	CCTNNNNNAGG	1 cut(s)	1153
XapI	RAATTY	5 cut(s)	542, 602, 1142, 1908, 1915
XceI	RCATGY	5 cut(s)	1508, 1868, 1900, 2281, 2305
XhoI	CTCGAG	1 cut(s)	876
XmiI	GTMKAC	1 cut(s)	1000
XmnI	GAANNNNTTC	1 cut(s)	1251
XspI	CTAG	8 cut(s)	147, 521, 1182, 1206, 1308, 1400, 1455, 1565
Zsp2I	ATGCAT	1 cut(s)	1563

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.