Rorug04G0383800

CWF19-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002851
Physical Location & Seq
Reverse (-)
55428701 .. 55432464
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0383800.1

Sequence Viewer

Length: 3483 bp
ATGGAAGCCCCAATTTCCGTCGAAAACCCTAAAACCTCCGAAGACCCAGAAACCTCCGATTCCACATTGCAAGTCGTCGACGACGGTGCCGTCGCCGTCGCCGCAGCCGGAGCCGACTCGATCCTCGACGTCTCCGGCCAAAGCCTGGACTTCCCGATCGGCGAGAACTCCGGCGACGACGTCGGGGATCTATACCTCTACAAAAATGTGTACAATTTACTGCCTAAATCCATCGCCGGACTAAAAGGCTTGAAGACGCTGAAGTTCTTCAGCAACGAGATCAATCTGTTCTCGTCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGAGGATCTCGTCGCCGGAGTTCGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTGGAGCTCTCCAAGGTCACATCTCGGTGCTCGGCGGTTCCGATTTTGAGCCAGATCGCCGGACTCAAGCTCCTCACGAAGCTATCGGTATGTTATTTCTCTATAACTTTCCTTCCTCCGGAGATTTGCTGCTTAAAGAGTTTGGAGCATCTGGATCTTTCGTTCAACAAGATGAAGACTTTGCCGGGAGAAATTGGCAATTTGAATGCTCTGGTGTCGTTGAGAGTTGCCAATAATAAATTGGAGGAATTGCCTGAGGGTTTGTCCAGATTAGCTAGGCTGGAGAACCTGGACTTGTCGCATAATAGGCTGACGTCATTGGGGGTTGTTGATTTTGGGTTGATGCTGAGCCTCCAGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTTGTGCCTTCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGTGTCTGGCAGTTCTTCAGTCGAGTTGGATTGCTCAGCGAATGTAATTAGTGTGTCTAGTAAATCGAGAAGTAGGTTTCTAGCAAGTTGGAGGTCGGATGGGAGGAAGTGTTATTTTCGGTGGAGAGCTCCAGGATGCGTAAATAAAGGCAGGATGCCCATGCCGATGCGTGTGGAGGAAGATGGAGAAAGTAAAACAGAAAATGTAGATGTTACTGATTCTGCAGTAGATGAGGATGAGGATGAAGGTTTAGTATCTCGAGAAGCTGAGATTTGTTCGATTGTTATCTCTACTGCAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCAAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCATTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAAGAAGCCGAATTGCAAGAATCCAAAATCTGGTGGGATTAGTGGTATATCTAGTTATGATATTTCACTAGATCAAGGTGATAAAAACAATTACGAGAATGAAGTATCCTCCTCAGCCAGTCAAGAGGTTGTCGGTGAGAAAGATGAAGAAGCATCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCAAATGGTGATGAGGATGTTAAGCAGCCAGATTGTGAGAATGGTGATGGGGATGTTAAGCAGCCAGACTGCAAGAATGGTGATGGGGATGTTAAGCAGCCAGACTGCAAGAATGTTGTTGGGGATAATAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTCAAAAATTTAGTGATACGTCTTTTTGCAGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGTGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCTTGACTCTCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGTGGTTCACCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTGTCAGATCATTTTGGAGGGAGTGATAGGGTTGCTGGTGTTGAACGGAGACGCAAAGGTGGTCCTGACGCAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACGGTGAACCTATTAATTTATCCACCAAACCATACTTGCAGACGGTTGAAGATAATGCTTTTGTTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACATAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCTTGTGAGCTTGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAATATTATACCAGTCAAGAAAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCAAATGACATGAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATTCCTATGACACGGATCAAAGTTCCTCCTTCTGGAAGCAACTTTGGTTGTTCTTTGCCACATTCTTTGCCCTCTTTTGATGGAACTCTGAACAAATCTGGCAGTTCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCACCACCCCTGCATAGTAGAGTTGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTTTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTGCATTTAGCTTTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGGACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAGTCGTACTTGCATTCCTGCCTTGTCGCTCATGTAGGAATACCTCCTCCTAATGTTTGCGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCATGAGCAAAATATTTATGGACTGGAAGTTGACATCTGGTCATTTGGATGCCTTCTTTCCGAATTGTTGACTTTGCAACTTCCTTACTCCGGAGTAGCTGATTTGGAGATACATGACCTTATTGTGACAGGACAACGACCTAAGTTAACGGATGAGTTGGAGGCGCTTCTGCTATTTATGGAGGAGCCAGTGGCTCGATCAGCTGGAGAGCTTGGGGGAACAGAGGCTGACTTAGACACACTAACATTTCTTGCTGACTTGTTCTGCAAATGTACTGAGGAGAATCCGCACAGTCGTCCAACAGCTGAGAACATCTACAAATTGTTGCTCGAACACAGCATGCAGCTTACAAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CwfJ_C_1 CwfJ_C_2

Protein Analysis

1160

Amino Acids

128.16

Weight (kDa)

5.09

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 119 - 259 3.7e-14 Leucine-rich repeat region
LRR_8 PF13855 156 - 212 2.4e-07 Leucine rich repeat
LRR_8 PF13855 202 - 259 5.5e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 700 - 773 1.3e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 873 - 1146 1.3e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 875 - 1092 6.2e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2045
AasI GACNNNNNNGTC 2 cut(s) 89, 2012
AatII GACGTC 3 cut(s) 132, 183, 710
Acc36I ACCTGC 1 cut(s) 2045
AccB1I GGYRCC 2 cut(s) 86, 3007
AccB7I CCANNNNNTGG 2 cut(s) 145, 1307
AccBSI CCGCTC 2 cut(s) 2139, 3005
AccI GTMKAC 1 cut(s) 78
AccIII TCCGGA 3 cut(s) 511, 1244, 3209
AciI CCGC 8 cut(s) 102, 365, 428, 1733, 1818, 2137, 3005, 3407
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 7 cut(s) 374, 751, 1477, 1660, 1673, 1777, 1842
AcuI CTGAAG 7 cut(s) 253, 281, 407, 837, 2577, 2700, 2999
AcyI GRCGYC 3 cut(s) 129, 180, 707
AfeI AGCGCT 1 cut(s) 384
AfiI CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1307, 2301, 2447, 3209
AhdI GACNNNNNGTC 1 cut(s) 3157
AjiI CACGTC 1 cut(s) 1739
AjnI CCWGG 5 cut(s) 144, 681, 967, 2280, 2731
AloI GAACNNNNNNTCC 2 cut(s) 539, 571
Alw21I GWGCWC 3 cut(s) 402, 425, 967
Alw26I GTCTC 4 cut(s) 136, 1981, 2333, 2910
AlwNI CAGNNNCTG 2 cut(s) 2396, 3347
Ama87I CYCGRG 2 cut(s) 1095, 2250
Aor13HI TCCGGA 3 cut(s) 511, 1244, 3209
Aor51HI AGCGCT 1 cut(s) 384
AoxI GGCC 2 cut(s) 136, 1745
ApoI RAATTY 7 cut(s) 374, 751, 1477, 1660, 1673, 1777, 1842
AseI ATTAAT 1 cut(s) 2064
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 2 cut(s) 385, 3286
AspS9I GGNCC 2 cut(s) 2000, 2970
AsuC2I CCSGG 1 cut(s) 579
AvaI CYCGRG 2 cut(s) 1095, 2250
AvaII GGWCC 2 cut(s) 2000, 2970
AxyI CCTNAGG 1 cut(s) 648
BamHI GGATCC 2 cut(s) 1247, 1463
BanI GGYRCC 2 cut(s) 86, 3007
BanII GRGCYC 3 cut(s) 402, 967, 2713
BarI GAAGNNNNNNTAC 2 cut(s) 2603, 2635
BauI CACGAG 1 cut(s) 1794
BbsI GAAGAC 4 cut(s) 48, 260, 575, 1703
Bbv12I GWGCWC 3 cut(s) 402, 425, 967
BbvCI CCTCAGC 1 cut(s) 1390
BceAI ACGGC 3 cut(s) 74, 80, 1732
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 5 cut(s) 146, 683, 969, 2282, 2733
BciVI GTATCC 3 cut(s) 1183, 1393, 2270
BclI TGATCA 1 cut(s) 1633
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 4 cut(s) 136, 1981, 2333, 2910
BfaI CTAG 9 cut(s) 323, 669, 894, 917, 1329, 1346, 2046, 2168, 3477
BfmI CTRYAG 3 cut(s) 1059, 1131, 2175
BfoI RGCGCY 2 cut(s) 386, 3287
BfuAI ACCTGC 1 cut(s) 2045
BfuI GTATCC 3 cut(s) 1183, 1393, 2270
BlpI GCTNAGC 2 cut(s) 740, 871
Bme1390I CCNGG 6 cut(s) 146, 579, 683, 969, 2282, 2733
Bme18I GGWCC 2 cut(s) 2000, 2970
BmeRI GACNNNNNGTC 1 cut(s) 3157
BmeT110I CYCGRG 2 cut(s) 1095, 2250
BmgBI CACGTC 1 cut(s) 1739
BmgT120I GGNCC 2 cut(s) 2000, 2970
BmrFI CCNGG 6 cut(s) 146, 579, 683, 969, 2282, 2733
BmsI GCATC 9 cut(s) 550, 726, 962, 981, 993, 1439, 1720, 2347, 3158
BpiI GAAGAC 4 cut(s) 48, 260, 575, 1703
BpmI CTGGAG 7 cut(s) 416, 695, 731, 951, 1781, 3084, 3345
Bpu10I CCTNAGC 2 cut(s) 1390, 2629
Bpu1102I GCTNAGC 2 cut(s) 740, 871
BpuEI CTTGAG 3 cut(s) 443, 746, 1262
BpuMI CCSGG 1 cut(s) 579
BsaBI GATNNNNATC 1 cut(s) 1121
BsaHI GRCGYC 3 cut(s) 129, 180, 707
BsaJI CCNNGG 1 cut(s) 405
BsaWI WCCGGW 3 cut(s) 511, 1244, 3209
BsaXI ACNNNNNCTCC 4 cut(s) 447, 477, 931, 961
Bsc4I CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1307, 2301, 2447, 3209
Bse1I ACTGG 6 cut(s) 1395, 1764, 2315, 2886, 3147, 3308
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 4 cut(s) 65, 1926, 2213, 2856
Bse8I GATNNNNATC 1 cut(s) 1121
BseAI TCCGGA 3 cut(s) 511, 1244, 3209
BseBI CCWGG 5 cut(s) 146, 683, 969, 2282, 2733
BseDI CCNNGG 1 cut(s) 405
BseJI GATNNNNATC 1 cut(s) 1121
BseLI CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1307, 2301, 2447, 3209
BseMI GCAATG 4 cut(s) 65, 1926, 2213, 2856
BseNI ACTGG 6 cut(s) 1395, 1764, 2315, 2886, 3147, 3308
BseRI GAGGAG 5 cut(s) 455, 1378, 3051, 3317, 3413
BsgI GTGCAG 3 cut(s) 353, 2207, 2862
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 138, 1747
BshNI GGYRCC 2 cut(s) 86, 3007
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 3 cut(s) 402, 425, 967
BsiHKCI CYCGRG 2 cut(s) 1095, 2250
BslFI GGGAC 4 cut(s) 1749, 2602, 2819, 2983
BslI CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1307, 2301, 2447, 3209
BsmAI GTCTC 4 cut(s) 136, 1981, 2333, 2910
BsmBI CGTCTC 2 cut(s) 136, 1981
BsmFI GGGAC 4 cut(s) 1749, 2602, 2819, 2983
BsmI GAATGC 4 cut(s) 332, 604, 2893, 3028
BsnI GGCC 2 cut(s) 138, 1747
BsoBI CYCGRG 2 cut(s) 1095, 2250
Bsp1286I GDGCHC 4 cut(s) 402, 425, 967, 2713
Bsp13I TCCGGA 3 cut(s) 511, 1244, 3209
Bsp1407I TGTACA 1 cut(s) 210
Bsp1720I GCTNAGC 2 cut(s) 740, 871
BspACI CCGC 8 cut(s) 102, 365, 428, 1733, 1818, 2137, 3005, 3407
BspANI GGCC 2 cut(s) 138, 1747
BspEI TCCGGA 3 cut(s) 511, 1244, 3209
BspHI TCATGA 1 cut(s) 1648
BspMAI CTGCAG 2 cut(s) 1063, 1135
BspMI ACCTGC 1 cut(s) 2045
BspT107I GGYRCC 2 cut(s) 86, 3007
BsrBI CCGCTC 2 cut(s) 2139, 3005
BsrDI GCAATG 4 cut(s) 65, 1926, 2213, 2856
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 6 cut(s) 1395, 1764, 2315, 2886, 3147, 3308
BssECI CCNNGG 1 cut(s) 405
BssNI GRCGYC 3 cut(s) 129, 180, 707
BssSI CACGAG 1 cut(s) 1794
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 1794
Bst2UI CCWGG 5 cut(s) 146, 683, 969, 2282, 2733
Bst4CI ACNGT 9 cut(s) 86, 359, 1184, 1709, 1874, 2054, 2095, 2185, 3413
Bst6I CTCTTC 1 cut(s) 3018
BstACI GRCGYC 3 cut(s) 129, 180, 707
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 3461
BstH2I RGCGCY 2 cut(s) 386, 3287
BstHHI GCGC 2 cut(s) 385, 3286
BstMAI GTCTC 4 cut(s) 136, 1981, 2333, 2910
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 7 cut(s) 101, 110, 391, 700, 1753, 2733, 3320
BstNI CCWGG 5 cut(s) 146, 683, 969, 2282, 2733
BstNSI RCATGY 4 cut(s) 1268, 2364, 2834, 3463
BstSCI CCNGG 6 cut(s) 144, 577, 681, 967, 2280, 2731
BstSFI CTRYAG 3 cut(s) 1059, 1131, 2175
BstV2I GAAGAC 4 cut(s) 48, 260, 575, 1703
BstX2I RGATCY 7 cut(s) 187, 336, 547, 1247, 1273, 1463, 2392
BstXI CCANNNNNNTGG 1 cut(s) 1864
BstYI RGATCY 7 cut(s) 187, 336, 547, 1247, 1273, 1463, 2392
Bsu36I CCTNAGG 1 cut(s) 648
BsuI GTATCC 3 cut(s) 1183, 1393, 2270
BsuRI GGCC 2 cut(s) 138, 1747
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 1739
BtsIMutI CAGTG 6 cut(s) 1757, 1899, 2537, 2724, 2879, 3315
BveI ACCTGC 1 cut(s) 2045
Cac8I GCNNGC 1 cut(s) 3461
CaiI CAGNNNCTG 2 cut(s) 2396, 3347
CciI TCATGA 1 cut(s) 1648
CfoI GCGC 2 cut(s) 385, 3286
Cfr13I GGNCC 2 cut(s) 2000, 2970
CseI GACGC 3 cut(s) 265, 1998, 2015
CspCI CAANNNNNGTGG 2 cut(s) 2463, 2498
DrdI GACNNNNNNGTC 2 cut(s) 89, 2012
DriI GACNNNNNGTC 1 cut(s) 3157
DseDI GACNNNNNNGTC 2 cut(s) 89, 2012
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 3018
Eam1105I GACNNNNNGTC 1 cut(s) 3157
EarI CTCTTC 1 cut(s) 3018
Ecl136II GAGCTC 2 cut(s) 400, 965
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 3 cut(s) 402, 967, 2713
Eco32I GATATC 2 cut(s) 2119, 2697
Eco47I GGWCC 2 cut(s) 2000, 2970
Eco47III AGCGCT 1 cut(s) 384
Eco53kI GAGCTC 2 cut(s) 400, 965
Eco57I CTGAAG 7 cut(s) 253, 281, 407, 837, 2577, 2700, 2999
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 2 cut(s) 1095, 2250
EcoICRI GAGCTC 2 cut(s) 400, 965
EcoO109I RGGNCCY 1 cut(s) 2970
EcoRI GAATTC 1 cut(s) 1477
EcoRII CCWGG 5 cut(s) 144, 681, 967, 2280, 2731
EcoRV GATATC 2 cut(s) 2119, 2697
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 3 cut(s) 332, 2362, 3123
EcoT38I GRGCYC 3 cut(s) 402, 967, 2713
ErhI CCWWGG 1 cut(s) 405
Esp3I CGTCTC 2 cut(s) 136, 1981
FaqI GGGAC 4 cut(s) 1749, 2602, 2819, 2983
FauI CCCGC 1 cut(s) 1726
FbaI TGATCA 1 cut(s) 1633
FblI GTMKAC 1 cut(s) 78
FriOI GRGCYC 3 cut(s) 402, 967, 2713
FspBI CTAG 9 cut(s) 323, 669, 894, 917, 1329, 1346, 2046, 2168, 3477
GlaI GCGC 2 cut(s) 384, 3285
GsuI CTGGAG 7 cut(s) 416, 695, 731, 951, 1781, 3084, 3345
HaeII RGCGCY 2 cut(s) 386, 3287
HaeIII GGCC 2 cut(s) 138, 1747
HgaI GACGC 3 cut(s) 265, 1998, 2015
HhaI GCGC 2 cut(s) 385, 3286
Hin1I GRCGYC 3 cut(s) 129, 180, 707
Hin6I GCGC 2 cut(s) 383, 3284
HinP1I GCGC 2 cut(s) 383, 3284
HincII GTYRAC 5 cut(s) 79, 2329, 3151, 3189, 3267
HindII GTYRAC 5 cut(s) 79, 2329, 3151, 3189, 3267
HpaI GTTAAC 1 cut(s) 3267
Hpy166II GTNNAC 8 cut(s) 79, 211, 1870, 2057, 2329, 3151, 3189, 3267
Hpy8I GTNNAC 8 cut(s) 79, 211, 1870, 2057, 2329, 3151, 3189, 3267
HpyAV CCTTC 9 cut(s) 382, 515, 807, 1076, 1136, 1252, 2454, 2724, 3182
HpyCH4III ACNGT 9 cut(s) 86, 359, 1184, 1709, 1874, 2054, 2095, 2185, 3413
HpyCH4IV ACGT 6 cut(s) 129, 180, 707, 1627, 1685, 1738
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 110, 391, 700, 1753, 2733, 3320
HpySE526I ACGT 6 cut(s) 129, 180, 707, 1627, 1685, 1738
Hsp92I GRCGYC 3 cut(s) 129, 180, 707
HspAI GCGC 2 cut(s) 383, 3284
KflI GGGWCCC 1 cut(s) 2970
Kpn2I TCCGGA 3 cut(s) 511, 1244, 3209
Ksp22I TGATCA 1 cut(s) 1633
KspAI GTTAAC 1 cut(s) 3267
LweI GCATC 9 cut(s) 550, 726, 962, 981, 993, 1439, 1720, 2347, 3158
MaeI CTAG 9 cut(s) 323, 669, 894, 917, 1329, 1346, 2046, 2168, 3477
MaeII ACGT 6 cut(s) 129, 180, 707, 1627, 1685, 1738
MaeIII GTNAC 4 cut(s) 409, 1048, 2795, 3244
MbiI CCGCTC 2 cut(s) 2139, 3005
MflI RGATCY 7 cut(s) 187, 336, 547, 1247, 1273, 1463, 2392
MhlI GDGCHC 4 cut(s) 402, 425, 967, 2713
MlyI GAGTC 5 cut(s) 110, 450, 1784, 2324, 2335
MmeI TCCRAC 8 cut(s) 843, 905, 912, 1815, 2327, 3058, 3258, 3443
Mph1103I ATGCAT 3 cut(s) 332, 2362, 3123
MroI TCCGGA 3 cut(s) 511, 1244, 3209
MroXI GAANNNNTTC 1 cut(s) 266
MslI CAYNNNNRTG 5 cut(s) 418, 1001, 1647, 2704, 3166
MspA1I CMGCKG 5 cut(s) 367, 1818, 2963, 3323, 3425
MspR9I CCNGG 6 cut(s) 146, 579, 683, 969, 2282, 2733
Mva1269I GAATGC 4 cut(s) 332, 604, 2893, 3028
MvaI CCWGG 5 cut(s) 146, 683, 969, 2282, 2733
MwoI GCNNNNNNNGC 7 cut(s) 101, 110, 391, 700, 1753, 2733, 3320
NciI CCSGG 1 cut(s) 579
NmeAIII GCCGAG 1 cut(s) 404
NmuCI GTSAC 2 cut(s) 409, 3244
NsiI ATGCAT 3 cut(s) 332, 2362, 3123
NspI RCATGY 4 cut(s) 1268, 2364, 2834, 3463
PaeI GCATGC 1 cut(s) 3463
PaeR7I CTCGAG 2 cut(s) 1095, 2250
PagI TCATGA 1 cut(s) 1648
PaqCI CACCTGC 1 cut(s) 2045
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 431
PctI GAATGC 4 cut(s) 332, 604, 2893, 3028
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 5 cut(s) 59, 757, 1055, 1297, 3403
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 2 cut(s) 145, 1307
PfoI TCCNGGA 1 cut(s) 967
Ple19I CGATCG 1 cut(s) 159
PleI GAGTC 5 cut(s) 110, 450, 1784, 2324, 2335
PpsI GAGTC 5 cut(s) 110, 450, 1784, 2324, 2335
PpuMI RGGWCCY 1 cut(s) 2970
PshBI ATTAAT 1 cut(s) 2064
Psp124BI GAGCTC 2 cut(s) 402, 967
Psp5II RGGWCCY 1 cut(s) 2970
Psp6I CCWGG 5 cut(s) 144, 681, 967, 2280, 2731
PspGI CCWGG 5 cut(s) 144, 681, 967, 2280, 2731
PspPI GGNCC 2 cut(s) 2000, 2970
PspPPI RGGWCCY 1 cut(s) 2970
PspXI VCTCGAGB 1 cut(s) 2250
PstI CTGCAG 2 cut(s) 1063, 1135
PstNI CAGNNNCTG 2 cut(s) 2396, 3347
PsuI RGATCY 7 cut(s) 187, 336, 547, 1247, 1273, 1463, 2392
PsyI GACNNNGTC 1 cut(s) 179
PvuI CGATCG 1 cut(s) 159
PvuII CAGCTG 3 cut(s) 2963, 3323, 3425
RseI CAYNNNNRTG 5 cut(s) 418, 1001, 1647, 2704, 3166
SacI GAGCTC 2 cut(s) 402, 967
SalI GTCGAC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 2000, 2970
SchI GAGTC 5 cut(s) 110, 450, 1784, 2324, 2335
ScrFI CCNGG 6 cut(s) 146, 579, 683, 969, 2282, 2733
SduI GDGCHC 4 cut(s) 402, 425, 967, 2713
SfaNI GCATC 9 cut(s) 550, 726, 962, 981, 993, 1439, 1720, 2347, 3158
SfcI CTRYAG 3 cut(s) 1059, 1131, 2175
Sfr274I CTCGAG 2 cut(s) 1095, 2250
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 2000, 2970
SlaI CTCGAG 2 cut(s) 1095, 2250
SmiMI CAYNNNNRTG 5 cut(s) 418, 1001, 1647, 2704, 3166
SmlI CTYRAG 5 cut(s) 458, 761, 1095, 1277, 2250
SmoI CTYRAG 5 cut(s) 458, 761, 1095, 1277, 2250
SphI GCATGC 1 cut(s) 3463
SsiI CCGC 8 cut(s) 102, 365, 428, 1733, 1818, 2137, 3005, 3407
SspI AATATT 3 cut(s) 2308, 2402, 3133
SspMI CTAG 9 cut(s) 323, 669, 894, 917, 1329, 1346, 2046, 2168, 3477
SstI GAGCTC 2 cut(s) 402, 967
StyD4I CCNGG 6 cut(s) 144, 577, 681, 967, 2280, 2731
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 9 cut(s) 86, 359, 1184, 1709, 1874, 2054, 2095, 2185, 3413
TaiI ACGT 6 cut(s) 132, 183, 710, 1630, 1688, 1741
TatI WGTACW 6 cut(s) 210, 765, 1639, 2619, 2769, 3392
TauI GCSGC 2 cut(s) 104, 367
TfiI GAWTC 5 cut(s) 59, 757, 1055, 1297, 3403
TscAI CASTG 6 cut(s) 1764, 1906, 2537, 2724, 2886, 3315
TseFI GTSAC 2 cut(s) 409, 3244
Tsp45I GTSAC 2 cut(s) 409, 3244
TspGWI ACGGA 4 cut(s) 7, 1999, 2443, 3284
TspRI CASTG 6 cut(s) 1764, 1906, 2537, 2724, 2886, 3315
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 2 cut(s) 145, 1307
VpaK11BI GGWCC 2 cut(s) 2000, 2970
VspI ATTAAT 1 cut(s) 2064
XapI RAATTY 7 cut(s) 374, 751, 1477, 1660, 1673, 1777, 1842
XceI RCATGY 4 cut(s) 1268, 2364, 2834, 3463
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XhoI CTCGAG 2 cut(s) 1095, 2250
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 9 cut(s) 323, 669, 894, 917, 1329, 1346, 2046, 2168, 3477
ZraI GACGTC 3 cut(s) 130, 181, 708
Zsp2I ATGCAT 3 cut(s) 332, 2362, 3123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.