Rorug03G0331500

CWF19-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002851
Physical Location & Seq
Reverse (-)
38456841 .. 38463127
6287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0331500.1

Sequence Viewer

Length: 1269 bp
ATGAAGAGAACAAGACAAGCCGGTCGACCTCGTACCATGGCTCAACTGTTGGAAGTAACTTCATCACGTTCTGGAAGCACAGGTCGCAGCGCAAACGAATCAGCACAGCTGCCTCTTCCAGTACCAGCAGTAGTAGAAGAGCAAGCCAATACGCCAACAAGTAAAGTCGCACGAGGACATAGTAAAGGCATTCCGGAATGGAATACAGGTGTTAAAGTACATGTTCAATTTAATGGAATCAATTTCCAGCCGGTGGGGGAAAGGGCAGGTCAGCTTAAGTCACAATTGGGTCAAATAGTACGAAATGGCCATAGGGTACCGCTAAATATTCTTGACTGGAAGAAAGTTGGTAATGATGTGAAGGAAGAAATTTGGGAAGAGGTTAAAAAAAACTTGGTGGAAGTCCCAGAAGGTTATAAATCAGTATGCTTGAAAAATTGTAATTTGCTTTGGAAGGACCATAAGAGCAAAACAAAATCATTTCACTATGTGCCCAACAAGGACGATCCTGAACTTGGTTCTAAAAATCCTCCTCATATTGTTGCAGAGCAATGGTCTGAGCTTGTTGCATATTGGAGTAGTGAGGATGCAAAGATGATAGCAGAAAAAAATTCAATTAATCGGGAGCTGCGTGGCCCATGTCACAAGACCGGTCGAAAACATTTTTCCCAAGTGCAATATGAGATGGAAAAAGATGGAGAACCAGTTGATAAGTTTAGTGTTTGGAAGAAAACACGTGACTTAAATGATCACCTTGTGCAAGAGTTCATTAAATCATATGAAGAGAAACTTAGAAAGGAGTCGGAGGAGGATCAAAACTTGACAAGTGTTAAAGACCGAATTTTCCACTCTTTGATGGGAGATGATGGTCATGGGTATTGTCGTACTTTTGGAGCTGGTGTACCTCGTAGTCTAGTATATCCAAAGGAGTCAAATATATCTCAAAGCACAAATGATCTCGTACAAAGAATCACTGAACAAGTGCGTCAAGAGTTTCAAGTGCAGCTTCAGCAACTGCATGCTCGAATTGATTTTCTGCAGAATAAGGACACTAGTACAGAGTCTCCTAGACAGATTGCTGATGCAACTAGTGGGCATGAAATTTTGAGGGAGTCTATAGGAGATGAAGTAACTCCAAATGCAAATCATGAACAGCCCTCATTGGAGCAGAATTCTCCAGTAATATCCCCTTCACAAAATGGATCTCAAAGGGTTCTCATGGCAAAAAGAGCAAAGCAAATGAAAGCAAGTGCAAAGCTCACAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CwfJ_C_1 CwfJ_C_2

Protein Analysis

422

Amino Acids

47.8

Weight (kDa)

9.0

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 183 - 301 2.5e-21 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AasI GACNNNNNNGTC 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 257
Acc65I GGTACC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 316
AccB7I CCANNNNNTGG 1 cut(s) 253
AccI GTMKAC 1 cut(s) 25
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 1 cut(s) 320
AclWI GGATC 3 cut(s) 500, 819, 1210
AcoI YGGCCR 1 cut(s) 307
AcsI RAATTY 5 cut(s) 369, 610, 840, 1101, 1171
AcuI CTGAAG 1 cut(s) 992
AcvI CACGTG 1 cut(s) 737
AdeI CACNNNGTG 2 cut(s) 490, 757
AfaI GTAC 9 cut(s) 34, 123, 219, 300, 318, 886, 903, 963, 1057
AfiI CCNNNNNNNGG 2 cut(s) 253, 515
AflII CTTAAG 1 cut(s) 275
AflIII ACRYGT 2 cut(s) 220, 734
AgeI ACCGGT 1 cut(s) 650
AgsI TTSAA 4 cut(s) 227, 433, 615, 998
AhlI ACTAGT 2 cut(s) 1052, 1088
AluBI AGCT 7 cut(s) 109, 274, 562, 628, 896, 1006, 1258
AluI AGCT 7 cut(s) 109, 274, 562, 628, 896, 1006, 1258
Alw26I GTCTC 1 cut(s) 1068
AlwI GGATC 3 cut(s) 500, 819, 1210
AlwNI CAGNNNCTG 1 cut(s) 1015
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 2 cut(s) 307, 634
ApeKI GCWGC 4 cut(s) 87, 109, 628, 1003
ApoI RAATTY 5 cut(s) 369, 610, 840, 1101, 1171
AseI ATTAAT 1 cut(s) 618
AsiGI ACCGGT 1 cut(s) 650
Asp718I GGTACC 1 cut(s) 316
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 457, 635
AsuHPI GGTGA 1 cut(s) 743
AvaII GGWCC 1 cut(s) 457
BaeGI GKGCMC 1 cut(s) 495
BalI TGGCCA 1 cut(s) 309
BanI GGYRCC 1 cut(s) 316
BauI CACGAG 1 cut(s) 171
BbrPI CACGTG 1 cut(s) 737
BbvI GCAGC 4 cut(s) 96, 99, 615, 1015
BccI CCATC 4 cut(s) 679, 689, 850, 860
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 1068
BcuI ACTAGT 2 cut(s) 1052, 1088
BfaI CTAG 4 cut(s) 914, 1053, 1068, 1089
BfmI CTRYAG 2 cut(s) 1037, 1116
BfrI CTTAAG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 257
BisI GCNGC 4 cut(s) 88, 110, 629, 1004
BlsI GCNGC 4 cut(s) 89, 111, 630, 1005
Bme18I GGWCC 1 cut(s) 457
BmgT120I GGNCC 2 cut(s) 457, 635
BmiI GGNNCC 1 cut(s) 318
BmsI GCATC 2 cut(s) 577, 1072
BpmI CTGGAG 1 cut(s) 1161
BsaAI YACGTR 1 cut(s) 737
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 2 cut(s) 193, 650
BsaXI ACNNNNNCTCC 8 cut(s) 690, 720, 885, 915, 1048, 1078, 1113, 1143
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 515
Bse118I RCCGGY 3 cut(s) 20, 250, 650
Bse1I ACTGG 4 cut(s) 119, 341, 704, 1178
Bse3DI GCAATG 1 cut(s) 557
BseAI TCCGGA 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 592
BseLI CCNNNNNNNGG 2 cut(s) 253, 515
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 4 cut(s) 119, 341, 704, 1178
BseRI GAGGAG 2 cut(s) 522, 821
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 4 cut(s) 96, 99, 615, 1015
BsgI GTGCAG 1 cut(s) 1022
Bsh1285I CGRYCG 2 cut(s) 25, 655
BshFI GGCC 2 cut(s) 309, 636
BshNI GGYRCC 1 cut(s) 316
BshTI ACCGGT 1 cut(s) 650
BsiEI CGRYCG 2 cut(s) 25, 655
BsiSI CCGG 4 cut(s) 21, 194, 251, 651
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 253, 515
BsmAI GTCTC 1 cut(s) 1068
BsmFI GGGAC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 2 cut(s) 309, 636
Bsp1286I GDGCHC 1 cut(s) 495
Bsp13I TCCGGA 1 cut(s) 193
Bsp143I GATC 5 cut(s) 505, 748, 811, 955, 1202
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 1 cut(s) 320
BspANI GGCC 2 cut(s) 309, 636
BspCNI CTCAG 1 cut(s) 550
BspEI TCCGGA 1 cut(s) 193
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 1 cut(s) 318
BspMAI CTGCAG 1 cut(s) 1041
BspMI ACCTGC 1 cut(s) 257
BspPI GGATC 3 cut(s) 500, 819, 1210
BspQI GCTCTTC 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 316
BspTI CTTAAG 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 557
BsrFI RCCGGY 3 cut(s) 20, 250, 650
BsrI ACTGG 4 cut(s) 119, 341, 704, 1178
BssAI RCCGGY 3 cut(s) 20, 250, 650
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 5 cut(s) 505, 748, 811, 955, 1202
BssSI CACGAG 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 48
Bst6I CTCTTC 4 cut(s) 120, 132, 372, 777
BstAFI CTTAAG 1 cut(s) 275
BstBAI YACGTR 1 cut(s) 737
BstC8I GCNNGC 2 cut(s) 144, 1020
BstDEI CTNAG 2 cut(s) 558, 791
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 592
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 5 cut(s) 508, 751, 814, 958, 1205
BstMAI GTCTC 1 cut(s) 1068
BstMBI GATC 5 cut(s) 505, 748, 811, 955, 1202
BstMCI CGRYCG 2 cut(s) 25, 655
BstMWI GCNNNNNNNGC 3 cut(s) 84, 1009, 1229
BstNSI RCATGY 2 cut(s) 224, 1022
BstSFI CTRYAG 2 cut(s) 1037, 1116
BstSLI GKGCMC 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 96, 99, 615, 1015
BstX2I RGATCY 1 cut(s) 1202
BstYI RGATCY 1 cut(s) 1202
BsuRI GGCC 2 cut(s) 309, 636
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 972
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 2 cut(s) 144, 1020
CaiI CAGNNNCTG 1 cut(s) 1015
CciI TCATGA 1 cut(s) 1147
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 3 cut(s) 20, 250, 650
Cfr13I GGNCC 2 cut(s) 457, 635
CseI GACGC 1 cut(s) 974
Csp6I GTAC 9 cut(s) 33, 122, 218, 299, 317, 885, 902, 962, 1056
CspAI ACCGGT 1 cut(s) 650
CviAII CATG 8 cut(s) 37, 221, 639, 872, 1019, 1097, 1148, 1219
CviQI GTAC 9 cut(s) 33, 122, 218, 299, 317, 885, 902, 962, 1056
DdeI CTNAG 2 cut(s) 558, 791
DpnI GATC 5 cut(s) 507, 750, 813, 957, 1204
DpnII GATC 5 cut(s) 505, 748, 811, 955, 1202
DraIII CACNNNGTG 2 cut(s) 490, 757
DrdI GACNNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 4 cut(s) 120, 132, 372, 777
EarI CTCTTC 4 cut(s) 120, 132, 372, 777
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 992
Eco72I CACGTG 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 1171
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 8 cut(s) 40, 224, 642, 875, 1022, 1100, 1151, 1222
FalI AAGNNNNNCTT 4 cut(s) 774, 806, 990, 1022
FaqI GGGAC 1 cut(s) 389
FatI CATG 8 cut(s) 36, 220, 638, 871, 1018, 1096, 1147, 1218
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 748
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 4 cut(s) 88, 110, 629, 1004
FokI GGATG 1 cut(s) 599
Fsp4HI GCNGC 4 cut(s) 88, 110, 629, 1004
FspBI CTAG 4 cut(s) 914, 1053, 1068, 1089
GlaI GCGC 1 cut(s) 91
GluI GCNGC 4 cut(s) 88, 110, 629, 1004
GsuI CTGGAG 1 cut(s) 1161
HaeIII GGCC 2 cut(s) 309, 636
HapII CCGG 4 cut(s) 21, 194, 251, 651
HgaI GACGC 1 cut(s) 974
HhaI GCGC 1 cut(s) 92
Hin1II CATG 8 cut(s) 40, 224, 642, 875, 1022, 1100, 1151, 1222
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 26
HindII GTYRAC 1 cut(s) 26
HinfI GANTC 7 cut(s) 98, 237, 800, 929, 969, 1061, 1112
HpaII CCGG 4 cut(s) 21, 194, 251, 651
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 2 cut(s) 26, 902
Hpy188I TCNGA 2 cut(s) 559, 805
Hpy188III TCNNGA 7 cut(s) 72, 194, 332, 509, 623, 989, 1148
Hpy8I GTNNAC 2 cut(s) 26, 902
HpyAV CCTTC 4 cut(s) 355, 404, 448, 1200
HpyCH4III ACNGT 1 cut(s) 48
HpyCH4IV ACGT 2 cut(s) 67, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 1009, 1229
HpyF3I CTNAG 2 cut(s) 558, 791
HpySE526I ACGT 2 cut(s) 67, 736
Hsp92II CATG 8 cut(s) 40, 224, 642, 875, 1022, 1100, 1151, 1222
HspAI GCGC 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 193
KpnI GGTACC 1 cut(s) 320
Ksp22I TGATCA 1 cut(s) 748
Kzo9I GATC 5 cut(s) 505, 748, 811, 955, 1202
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 3 cut(s) 625, 893, 1165
Lsp1109I GCAGC 4 cut(s) 96, 99, 615, 1015
LweI GCATC 2 cut(s) 577, 1072
MaeI CTAG 4 cut(s) 914, 1053, 1068, 1089
MaeII ACGT 2 cut(s) 67, 736
MaeIII GTNAC 5 cut(s) 55, 279, 641, 737, 1129
MalI GATC 5 cut(s) 507, 750, 813, 957, 1204
MboI GATC 5 cut(s) 505, 748, 811, 955, 1202
MboII GAAGA 8 cut(s) 16, 107, 149, 352, 377, 389, 739, 794
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 1 cut(s) 1202
MhlI GDGCHC 1 cut(s) 495
MlsI TGGCCA 1 cut(s) 309
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 4 cut(s) 809, 938, 1070, 1121
MmeI TCCRAC 2 cut(s) 30, 783
Mox20I TGGCCA 1 cut(s) 309
MroI TCCGGA 1 cut(s) 193
MscI TGGCCA 1 cut(s) 309
MseI TTAA 8 cut(s) 213, 231, 276, 384, 618, 743, 771, 831
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 109
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 4 cut(s) 21, 194, 251, 651
MunI CAATTG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 84, 1009, 1229
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 778
NdeII GATC 5 cut(s) 505, 748, 811, 955, 1202
NlaIII CATG 8 cut(s) 40, 224, 642, 875, 1022, 1100, 1151, 1222
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 3 cut(s) 279, 641, 737
NspI RCATGY 2 cut(s) 224, 1022
PaeI GCATGC 1 cut(s) 1022
PagI TCATGA 1 cut(s) 1147
PciI ACATGT 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 628
PctI GAATGC 1 cut(s) 189
PfeI GAWTC 3 cut(s) 98, 237, 969
PflMI CCANNNNNTGG 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 650
PkrI GCNGC 4 cut(s) 89, 111, 630, 1005
PleI GAGTC 4 cut(s) 808, 937, 1069, 1120
PmaCI CACGTG 1 cut(s) 737
PmlI CACGTG 1 cut(s) 737
PpsI GAGTC 4 cut(s) 808, 937, 1069, 1120
Ppu21I YACGTR 1 cut(s) 737
PscI ACATGT 1 cut(s) 220
PshBI ATTAAT 1 cut(s) 618
PsiI TTATAA 1 cut(s) 417
PspCI CACGTG 1 cut(s) 737
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 457, 635
PstI CTGCAG 1 cut(s) 1041
PstNI CAGNNNCTG 1 cut(s) 1015
PsuI RGATCY 1 cut(s) 1202
PvuII CAGCTG 1 cut(s) 109
RsaI GTAC 9 cut(s) 34, 123, 219, 300, 318, 886, 903, 963, 1057
RsaNI GTAC 9 cut(s) 33, 122, 218, 299, 317, 885, 902, 962, 1056
SalI GTCGAC 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 8 cut(s) 213, 231, 276, 384, 618, 743, 771, 831
SatI GCNGC 4 cut(s) 88, 110, 629, 1004
Sau3AI GATC 5 cut(s) 505, 748, 811, 955, 1202
Sau96I GGNCC 2 cut(s) 457, 635
SchI GAGTC 4 cut(s) 809, 938, 1070, 1121
SduI GDGCHC 1 cut(s) 495
SfaNI GCATC 2 cut(s) 577, 1072
SfcI CTRYAG 2 cut(s) 1037, 1116
SinI GGWCC 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SpeI ACTAGT 2 cut(s) 1052, 1088
SphI GCATGC 1 cut(s) 1022
SsiI CCGC 1 cut(s) 320
SspI AATATT 1 cut(s) 328
SspMI CTAG 4 cut(s) 914, 1053, 1068, 1089
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 48
TaiI ACGT 2 cut(s) 70, 739
TaqI TCGA 3 cut(s) 25, 655, 1024
TaqII GACCGA 1 cut(s) 852
TatI WGTACW 2 cut(s) 217, 1055
TfiI GAWTC 3 cut(s) 98, 237, 969
Tru1I TTAA 8 cut(s) 213, 231, 276, 384, 618, 743, 771, 831
Tru9I TTAA 8 cut(s) 213, 231, 276, 384, 618, 743, 771, 831
TscAI CASTG 1 cut(s) 979
TseFI GTSAC 3 cut(s) 279, 641, 737
TseI GCWGC 4 cut(s) 87, 109, 628, 1003
Tsp45I GTSAC 3 cut(s) 279, 641, 737
TspDTI ATGAA 8 cut(s) 17, 51, 757, 795, 1113, 1140, 1164, 1256
TspRI CASTG 1 cut(s) 979
Van91I CCANNNNNTGG 1 cut(s) 253
Vha464I CTTAAG 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 457
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 5 cut(s) 369, 610, 840, 1101, 1171
XceI RCATGY 2 cut(s) 224, 1022
XmiI GTMKAC 1 cut(s) 25
XspI CTAG 4 cut(s) 914, 1053, 1068, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.