FvH4_4g05010

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009256
Physical Location & Seq
Reverse (-)
4312768 .. 4314590
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05010.t1

Sequence Viewer

Length: 642 bp
ATGGTAGGTGGTGGAAGAAGTGCAGCGCCAGGTCGGAAAATAATGGTGGTGGCCGATCCAACCCAAGAGTCAGCAGGTGCACTCCAATATGCTCTTTCTCAAGCAGTTGTTGACAATGACGAATTGATCCTCTTCCATGTTGAAAACCCTAATTCGTGGCGGAACACGATCTCCATGTTCCTAAAGAAACCCGGCCTCATGAATTCAGGCCCTACCAGCGCCAGTGCCCGTGCCGGAGCCGGTGCCGGCGCCACCACATCTGTAGAAGGAAGCTTTGGAAGAGGGGACGTGGAATTTCTTGAAGAAATGAAAAGTGCATGCAAGCTTTGCAAACCAAAACTTCGCGTTCGGATTGAGAAGAAGGCGATGGCGGAAGGCAAAGACAAAGCAACCGTTATTCTAGATCAAACCAAAGTTCTTGGTATCGATGTTCTGATTATAGGACAACGAAAGAGCCTTTCTACAATTCTTTTAGGATATAGGCTGCCTGGAGCATCGTCGCGAGCGACAAAACCAATTGACACAGCAGAGTATTTGATTGATAACAGCAACTGCACTTGTGTAGCAGTACAGAAAAAGGGACAAAATGGAGGTTACCTTCTCAACACAAAAACCCATAAAAACTTTTGGCTTCTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

214

Amino Acids

22.74

Weight (kDa)

9.47

Isoelectric Point (pI)

22.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 12 - 159 3.3e-07 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 65
AccB1I GGYRCC 2 cut(s) 242, 248
AccII CGCG 2 cut(s) 345, 502
AciI CCGC 2 cut(s) 160, 371
AclWI GGATC 2 cut(s) 50, 121
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 2 cut(s) 202, 293
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 1 cut(s) 570
AgsI TTSAA 2 cut(s) 143, 302
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 2 cut(s) 28, 487
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 2 cut(s) 273, 325
AluI AGCT 2 cut(s) 273, 325
Alw21I GWGCWC 1 cut(s) 82
Alw44I GTGCAC 1 cut(s) 78
AlwI GGATC 2 cut(s) 50, 121
AlwNI CAGNNNCTG 1 cut(s) 552
AoxI GGCC 3 cut(s) 51, 193, 208
ApaLI GTGCAC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 23, 484
ApoI RAATTY 2 cut(s) 202, 293
AspLEI GCGC 3 cut(s) 28, 221, 251
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 1 cut(s) 192
BaeGI GKGCMC 2 cut(s) 82, 229
BanI GGYRCC 2 cut(s) 242, 248
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 2 cut(s) 35, 471
BccI CCATC 1 cut(s) 361
BciT130I CCWGG 2 cut(s) 30, 489
BcnI CCSGG 1 cut(s) 192
BfaI CTAG 2 cut(s) 401, 635
BfmI CTRYAG 1 cut(s) 261
BfoI RGCGCY 3 cut(s) 29, 222, 252
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 2 cut(s) 24, 485
BlsI GCNGC 2 cut(s) 25, 486
Bme1390I CCNGG 3 cut(s) 30, 192, 489
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 3 cut(s) 238, 244, 250
BmrFI CCNGG 3 cut(s) 30, 192, 489
BmsI GCATC 1 cut(s) 503
BpmI CTGGAG 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 192
Bsa29I ATCGAT 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bse118I RCCGGY 2 cut(s) 239, 245
Bse1I ACTGG 1 cut(s) 222
BseBI CCWGG 2 cut(s) 30, 489
BseCI ATCGAT 1 cut(s) 426
BseNI ACTGG 1 cut(s) 222
BseSI GKGCMC 2 cut(s) 82, 229
BseXI GCAGC 2 cut(s) 35, 471
BsgI GTGCAG 2 cut(s) 42, 538
Bsh1236I CGCG 2 cut(s) 345, 502
BshFI GGCC 3 cut(s) 53, 195, 210
BshNI GGYRCC 2 cut(s) 242, 248
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 82
BsiSI CCGG 4 cut(s) 192, 234, 240, 246
BslFI GGGAC 2 cut(s) 299, 594
BsmFI GGGAC 2 cut(s) 299, 594
BsnI GGCC 3 cut(s) 53, 195, 210
Bsp1286I GDGCHC 2 cut(s) 82, 229
Bsp143I GATC 4 cut(s) 55, 126, 168, 403
Bsp68I TCGCGA 1 cut(s) 502
BspACI CCGC 2 cut(s) 160, 371
BspANI GGCC 3 cut(s) 53, 195, 210
BspDI ATCGAT 1 cut(s) 426
BspFNI CGCG 2 cut(s) 345, 502
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 238, 244, 250
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 2 cut(s) 50, 121
BspT107I GGYRCC 2 cut(s) 242, 248
BsrFI RCCGGY 2 cut(s) 239, 245
BsrI ACTGG 1 cut(s) 222
BssAI RCCGGY 2 cut(s) 239, 245
BssMI GATC 4 cut(s) 55, 126, 168, 403
BssNI GRCGYC 1 cut(s) 249
Bst2UI CCWGG 2 cut(s) 30, 489
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 2 cut(s) 137, 274
BstACI GRCGYC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 327
BstC8I GCNNGC 4 cut(s) 247, 319, 323, 504
BstEII GGTNACC 1 cut(s) 593
BstFNI CGCG 2 cut(s) 345, 502
BstH2I RGCGCY 3 cut(s) 29, 222, 252
BstHHI GCGC 3 cut(s) 28, 221, 251
BstKTI GATC 4 cut(s) 58, 129, 171, 406
BstMBI GATC 4 cut(s) 55, 126, 168, 403
BstMWI GCNNNNNNNGC 2 cut(s) 216, 327
BstNI CCWGG 2 cut(s) 30, 489
BstNSI RCATGY 1 cut(s) 321
BstPI GGTNACC 1 cut(s) 593
BstSCI CCNGG 3 cut(s) 28, 190, 487
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 2 cut(s) 82, 229
BstUI CGCG 2 cut(s) 345, 502
BstV1I GCAGC 2 cut(s) 35, 471
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 3 cut(s) 53, 195, 210
BsuTUI ATCGAT 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 380
BtrI CACGTC 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 229
BtuMI TCGCGA 1 cut(s) 502
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 4 cut(s) 247, 319, 323, 504
CaiI CAGNNNCTG 1 cut(s) 552
CciI TCATGA 1 cut(s) 198
CfoI GCGC 3 cut(s) 28, 221, 251
Cfr10I RCCGGY 2 cut(s) 239, 245
Cfr13I GGNCC 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 1 cut(s) 569
CviAII CATG 4 cut(s) 137, 175, 199, 318
CviJI RGCY 9 cut(s) 53, 195, 210, 239, 273, 325, 456, 484, 631
CviKI_1 RGCY 9 cut(s) 53, 195, 210, 239, 273, 325, 456, 484, 631
CviQI GTAC 1 cut(s) 569
DinI GGCGCC 1 cut(s) 250
DpnI GATC 4 cut(s) 57, 128, 170, 405
DpnII GATC 4 cut(s) 55, 126, 168, 403
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 137, 274
EarI CTCTTC 2 cut(s) 137, 274
EciI GGCGGA 2 cut(s) 175, 386
Eco91I GGTNACC 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 593
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 28, 487
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
FaeI CATG 4 cut(s) 140, 178, 202, 321
FaiI YATR 9 cut(s) 90, 138, 176, 200, 319, 440, 480, 618, 640
FaqI GGGAC 2 cut(s) 299, 594
FatI CATG 4 cut(s) 136, 174, 198, 317
Fnu4HI GCNGC 2 cut(s) 24, 485
Fsp4HI GCNGC 2 cut(s) 24, 485
FspBI CTAG 2 cut(s) 401, 635
GlaI GCGC 3 cut(s) 27, 220, 250
GluI GCNGC 2 cut(s) 24, 485
GsuI CTGGAG 1 cut(s) 510
HaeII RGCGCY 3 cut(s) 29, 222, 252
HaeIII GGCC 3 cut(s) 53, 195, 210
HapII CCGG 4 cut(s) 192, 234, 240, 246
HhaI GCGC 3 cut(s) 28, 221, 251
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 4 cut(s) 140, 178, 202, 321
Hin6I GCGC 3 cut(s) 26, 219, 249
HinP1I GCGC 3 cut(s) 26, 219, 249
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 2 cut(s) 271, 323
HinfI GANTC 1 cut(s) 68
HpaII CCGG 4 cut(s) 192, 234, 240, 246
Hpy166II GTNNAC 2 cut(s) 80, 112
Hpy188I TCNGA 3 cut(s) 36, 351, 435
Hpy188III TCNNGA 4 cut(s) 199, 299, 401, 501
Hpy8I GTNNAC 2 cut(s) 80, 112
Hpy99I CGWCG 1 cut(s) 502
HpyAV CCTTC 4 cut(s) 260, 355, 368, 608
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 6 cut(s) 23, 80, 317, 321, 330, 555
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 327
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 4 cut(s) 140, 178, 202, 321
HspAI GCGC 3 cut(s) 26, 219, 249
KasI GGCGCC 1 cut(s) 248
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
Kzo9I GATC 4 cut(s) 55, 126, 168, 403
LmnI GCTCC 2 cut(s) 236, 491
Lsp1109I GCAGC 2 cut(s) 35, 471
LweI GCATC 1 cut(s) 503
MaeI CTAG 2 cut(s) 401, 635
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 593
MalI GATC 4 cut(s) 57, 128, 170, 405
MboI GATC 4 cut(s) 55, 126, 168, 403
MboII GAAGA 5 cut(s) 27, 124, 291, 314, 370
MfeI CAATTG 1 cut(s) 516
MhlI GDGCHC 2 cut(s) 82, 229
MluCI AATT 6 cut(s) 122, 151, 202, 293, 465, 516
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 14, 83
MnlI CCTC 4 cut(s) 140, 206, 275, 584
MroNI GCCGGC 1 cut(s) 245
MspI CCGG 4 cut(s) 192, 234, 240, 246
MspR9I CCNGG 3 cut(s) 30, 192, 489
MunI CAATTG 1 cut(s) 516
MvaI CCWGG 2 cut(s) 30, 489
MvnI CGCG 2 cut(s) 345, 502
MwoI GCNNNNNNNGC 2 cut(s) 216, 327
NaeI GCCGGC 1 cut(s) 247
NarI GGCGCC 1 cut(s) 249
NciI CCSGG 1 cut(s) 192
NdeII GATC 4 cut(s) 55, 126, 168, 403
NgoMIV GCCGGC 1 cut(s) 245
NlaIII CATG 4 cut(s) 140, 178, 202, 321
NlaIV GGNNCC 3 cut(s) 238, 244, 250
NruI TCGCGA 1 cut(s) 502
NspI RCATGY 1 cut(s) 321
PaeI GCATGC 1 cut(s) 321
PagI TCATGA 1 cut(s) 198
PaqCI CACCTGC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PdiI GCCGGC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 25, 486
PleI GAGTC 1 cut(s) 76
PluTI GGCGCC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 28, 487
PspEI GGTNACC 1 cut(s) 593
PspGI CCWGG 2 cut(s) 28, 487
PspN4I GGNNCC 3 cut(s) 238, 244, 250
PspPI GGNCC 1 cut(s) 209
PstNI CAGNNNCTG 1 cut(s) 552
RruI TCGCGA 1 cut(s) 502
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SatI GCNGC 2 cut(s) 24, 485
Sau3AI GATC 4 cut(s) 55, 126, 168, 403
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 3 cut(s) 30, 192, 489
SduI GDGCHC 2 cut(s) 82, 229
SetI ASST 8 cut(s) 10, 34, 79, 275, 291, 327, 595, 600
SfaNI GCATC 1 cut(s) 503
SfcI CTRYAG 1 cut(s) 261
SfoI GGCGCC 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
SphI GCATGC 1 cut(s) 321
Sse9I AATT 6 cut(s) 122, 151, 202, 293, 465, 516
SsiI CCGC 2 cut(s) 160, 371
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 2 cut(s) 401, 635
StyD4I CCNGG 3 cut(s) 28, 190, 487
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 426
TasI AATT 6 cut(s) 122, 151, 202, 293, 465, 516
TatI WGTACW 1 cut(s) 568
TscAI CASTG 1 cut(s) 229
TseI GCWGC 2 cut(s) 23, 484
TspDTI ATGAA 2 cut(s) 215, 323
TspRI CASTG 1 cut(s) 229
VneI GTGCAC 1 cut(s) 78
XapI RAATTY 2 cut(s) 202, 293
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 1 cut(s) 321
XspI CTAG 2 cut(s) 401, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.