MD16G1227600.v1.1

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009256
Physical Location & Seq
Reverse (-)
23162099 .. 23163212
1114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1227600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGTGGTGTATCAATACCTGCTGGACATGGACTATGGACGTAATGCATCGCCACGGAGAATAATGGTGGTAGCAGATCCAACACAAGAGTCAGCAGGTGCACTCCAATATGCACTCTCTAAAGCGGTTCTCGAGAACGATGAATTGATCCTCTTCCATATTGAAAACCCTAATTCATGGCGTTACACAATAACAACGTTCCTTAGAAGACCTAGGGGCTGCTCCACCACCGCATCTGTAGAAGGAAGCTTAGGGAACACGGAGTTTCTTGAAGAAATGAAGAATGCATGCAAACTTGCAAAACCAAAAGTGCGTGTGCGGGTAGAGAAGGTGGCACTAATGGAAGGCAAGGACAAAGCAAGTATTATTCTAGATCAAAGCAAGGTTCTTGGGGTGAATGTCCTTGTTATAGGGCAACGACGGAGTCTTTCTACAGTTATCTTAGGGAGGCCTGGAGGTTCCACGCGAGGGGCAAAACCAGTAGACACAGCAGAGTATCTGATTGATAAAAGCAGTTGCACTTGTGTGGGAGTACAGAAAAAGGGCCAAAACGGAGGCTACCTTCTCAATACAAAGACCCACAAAAACTTCTGGCTTCTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

202

Amino Acids

22.21

Weight (kDa)

9.47

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 19 - 149 4.5e-06 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 2 cut(s) 27, 86
AccI GTMKAC 1 cut(s) 483
AccII CGCG 1 cut(s) 466
AciI CCGC 3 cut(s) 125, 231, 319
AclI AACGTT 1 cut(s) 197
AclWI GGATC 2 cut(s) 71, 142
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 1 cut(s) 468
AgsI TTSAA 2 cut(s) 164, 272
AjnI CCWGG 1 cut(s) 451
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 1 cut(s) 249
AluI AGCT 1 cut(s) 249
Alw21I GWGCWC 1 cut(s) 103
Alw44I GTGCAC 1 cut(s) 99
AlwI GGATC 2 cut(s) 71, 142
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 449, 544
ApaLI GTGCAC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 219
AspA2I CCTAGG 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 131
AvrII CCTAGG 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 453
BfaI CTAG 2 cut(s) 213, 371
BfmI CTRYAG 2 cut(s) 237, 432
BfuAI ACCTGC 2 cut(s) 27, 86
BisI GCNGC 1 cut(s) 220
BlnI CCTAGG 1 cut(s) 212
BlsI GCNGC 1 cut(s) 221
Bme1390I CCNGG 1 cut(s) 453
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 2 cut(s) 56, 242
BpiI GAAGAC 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 53, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 468
Bse1I ACTGG 1 cut(s) 479
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 2 cut(s) 53, 212
BseLI CCNNNNNNNGG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 1 cut(s) 206
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 2 cut(s) 451, 546
BsiHKAI GWGCWC 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 468
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 2 cut(s) 451, 546
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 3 cut(s) 76, 147, 373
BspACI CCGC 3 cut(s) 125, 231, 319
BspANI GGCC 2 cut(s) 451, 546
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 460
BspMI ACCTGC 2 cut(s) 27, 86
BspPI GGATC 2 cut(s) 71, 142
BsrI ACTGG 1 cut(s) 479
BssECI CCNNGG 2 cut(s) 53, 212
BssMI GATC 3 cut(s) 76, 147, 373
BssT1I CCWWGG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 203, 250, 442
BstDSI CCRYGG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 466
BstKTI GATC 3 cut(s) 79, 150, 376
BstMBI GATC 3 cut(s) 76, 147, 373
BstNI CCWGG 1 cut(s) 453
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 2 cut(s) 237, 432
BstSLI GKGCMC 1 cut(s) 103
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 2 cut(s) 451, 546
BtgI CCRYGG 1 cut(s) 53
BtgZI GCGATG 1 cut(s) 33
BveI ACCTGC 2 cut(s) 27, 86
Cac8I GCNNGC 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 1 cut(s) 533
CviAII CATG 3 cut(s) 28, 177, 288
CviJI RGCY 7 cut(s) 219, 249, 451, 546, 558, 595, 602
CviKI_1 RGCY 7 cut(s) 219, 249, 451, 546, 558, 595, 602
CviQI GTAC 1 cut(s) 533
DdeI CTNAG 3 cut(s) 203, 250, 442
DpnI GATC 3 cut(s) 78, 149, 375
DpnII GATC 3 cut(s) 76, 147, 373
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 212
Eco147I AGGCCT 1 cut(s) 451
Eco88I CYCGRG 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 212
EcoT22I ATGCAT 2 cut(s) 49, 289
ErhI CCWWGG 1 cut(s) 212
FaeI CATG 3 cut(s) 31, 180, 291
FaiI YATR 7 cut(s) 29, 36, 111, 159, 178, 289, 410
FatI CATG 3 cut(s) 27, 176, 287
FauI CCCGC 1 cut(s) 312
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 1 cut(s) 220
Fsp4HI GCNGC 1 cut(s) 220
FspBI CTAG 2 cut(s) 213, 371
GluI GCNGC 1 cut(s) 220
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 451, 546
Hin1II CATG 3 cut(s) 31, 180, 291
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 2 cut(s) 89, 424
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 2 cut(s) 101, 484
Hpy188I TCNGA 1 cut(s) 501
Hpy188III TCNNGA 4 cut(s) 131, 133, 269, 371
Hpy8I GTNNAC 2 cut(s) 101, 484
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 4 cut(s) 236, 322, 338, 572
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 40, 197
HpyCH4V TGCA 7 cut(s) 47, 101, 113, 287, 291, 299, 519
HpyF3I CTNAG 3 cut(s) 203, 250, 442
HpySE526I ACGT 2 cut(s) 40, 197
Hsp92II CATG 3 cut(s) 31, 180, 291
Kzo9I GATC 3 cut(s) 76, 147, 373
LmnI GCTCC 1 cut(s) 227
LpnPI CCDG 8 cut(s) 8, 32, 81, 438, 465, 492, 577, 584
Lsp1109I GCAGC 1 cut(s) 206
LweI GCATC 2 cut(s) 56, 242
MaeI CTAG 2 cut(s) 213, 371
MaeII ACGT 2 cut(s) 40, 197
MaeIII GTNAC 1 cut(s) 182
MalI GATC 3 cut(s) 78, 149, 375
MboI GATC 3 cut(s) 76, 147, 373
MboII GAAGA 4 cut(s) 145, 219, 284, 292
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 2 cut(s) 143, 172
MlyI GAGTC 2 cut(s) 98, 433
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 5 cut(s) 161, 441, 449, 461, 548
Mph1103I ATGCAT 2 cut(s) 49, 289
MseI TTAA 1 cut(s) 604
MslI CAYNNNNRTG 1 cut(s) 524
MspR9I CCNGG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 1 cut(s) 466
NdeII GATC 3 cut(s) 76, 147, 373
NlaIII CATG 3 cut(s) 31, 180, 291
NlaIV GGNNCC 1 cut(s) 460
NsiI ATGCAT 2 cut(s) 49, 289
NspI RCATGY 1 cut(s) 291
OliI CACNNNNGTG 1 cut(s) 524
PaeI GCATGC 1 cut(s) 291
PaeR7I CTCGAG 1 cut(s) 131
PaqCI CACCTGC 1 cut(s) 86
PceI AGGCCT 1 cut(s) 451
PctI GAATGC 1 cut(s) 289
PflFI GACNNNGTC 1 cut(s) 423
PkrI GCNGC 1 cut(s) 221
PleI GAGTC 2 cut(s) 97, 432
PpsI GAGTC 2 cut(s) 97, 432
Psp1406I AACGTT 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 1 cut(s) 544
PsuI RGATCY 1 cut(s) 76
PsyI GACNNNGTC 1 cut(s) 423
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 1 cut(s) 604
SatI GCNGC 1 cut(s) 220
Sau3AI GATC 3 cut(s) 76, 147, 373
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 2 cut(s) 98, 433
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 103
SfaNI GCATC 2 cut(s) 56, 242
SfcI CTRYAG 2 cut(s) 237, 432
Sfr274I CTCGAG 1 cut(s) 131
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 291
Sse9I AATT 2 cut(s) 143, 172
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 3 cut(s) 125, 231, 319
SspMI CTAG 2 cut(s) 213, 371
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 212
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 2 cut(s) 43, 200
TaqI TCGA 1 cut(s) 132
TasI AATT 2 cut(s) 143, 172
TatI WGTACW 1 cut(s) 532
Tru1I TTAA 1 cut(s) 604
Tru9I TTAA 1 cut(s) 604
TseI GCWGC 1 cut(s) 219
TspDTI ATGAA 3 cut(s) 156, 165, 293
TspGWI ACGGA 4 cut(s) 70, 275, 436, 567
Tth111I GACNNNGTC 1 cut(s) 423
VneI GTGCAC 1 cut(s) 99
XbaI TCTAGA 1 cut(s) 370
XceI RCATGY 1 cut(s) 291
XhoI CTCGAG 1 cut(s) 131
XmaJI CCTAGG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 2 cut(s) 213, 371
Zsp2I ATGCAT 2 cut(s) 49, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.