MD13G1223300.v1.1

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009256
Physical Location & Seq
Reverse (-)
21750620 .. 21752918
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1223300.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGACTTTCGACATAATGCATCGCCGCGGAGAATAATGGTGGTAGCAGATCCAACCCAAGAGTCAGCAGGTGCACTCCAATATGCACTGTCTAAAACTGTTTCCGAGAACGATGAATTGATTCTCTTCCATGTCGAAAACCCTAATTCATGGCGATACACGATAACGATGTTCCTTAAAAGACCTAGGGGTGGCTCCGCCACCGTAGAAGGAGGTTTAGGGAACACGGAGTTTCTTGAAGAAATGAAGAATGCATGCAAACATGCAAAACCAAAAGTACGTGTGCGGGTAGAGAAGGTGGCGTTAATGGAAGGCAAGGACAAAGCAAGTATTATTCTAGATCAAAGCAAGGTTCTTGGGGTGGACGTCCTTGTTATAGGGCAACGACGGAGTCTTTCTACAGTTATCTTAGGATACAGGAGGCCTGGAGGTTCCACGCGAGGGACAAAACCAATAGACACAGCAGAGTATTTGATTGATAAAAGCAGTTGCACCTGCGTCGGAGTACAGAAAAAGGGACAAAATGGAGGCTACCTTCTCAATACAAAGACCCACAAAAATTTCTGGCTTCTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

193

Amino Acids

21.26

Weight (kDa)

9.65

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 10 - 138 1.5e-07 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 59, 503
AatII GACGTC 1 cut(s) 369
Acc36I ACCTGC 2 cut(s) 59, 503
AccII CGCG 2 cut(s) 28, 439
AciI CCGC 4 cut(s) 26, 28, 198, 286
AclWI GGATC 1 cut(s) 44
AcsI RAATTY 1 cut(s) 559
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 2 cut(s) 279, 507
AfiI CCNNNNNNNGG 2 cut(s) 191, 441
AflIII ACRYGT 1 cut(s) 280
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 1 cut(s) 424
Alw21I GWGCWC 1 cut(s) 76
Alw44I GTGCAC 1 cut(s) 72
AlwI GGATC 1 cut(s) 44
AoxI GGCC 1 cut(s) 422
ApaLI GTGCAC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 559
Asp700I GAANNNNTTC 1 cut(s) 120
AspA2I CCTAGG 1 cut(s) 185
AvrII CCTAGG 1 cut(s) 185
BaeGI GKGCMC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 76
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 1 cut(s) 426
BciVI GTATCC 1 cut(s) 407
BfaI CTAG 2 cut(s) 186, 338
BfmI CTRYAG 1 cut(s) 399
BfuAI ACCTGC 2 cut(s) 59, 503
BfuI GTATCC 1 cut(s) 407
BisI GCNGC 1 cut(s) 26
BlnI CCTAGG 1 cut(s) 185
BlsI GCNGC 1 cut(s) 27
Bme1390I CCNGG 1 cut(s) 426
BmiI GGNNCC 2 cut(s) 196, 433
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 26, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 441
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 26, 185
BseLI CCNNNNNNNGG 2 cut(s) 191, 441
BseSI GKGCMC 1 cut(s) 76
Bsh1236I CGCG 2 cut(s) 28, 439
BshFI GGCC 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 76
BslFI GGGAC 2 cut(s) 457, 531
BslI CCNNNNNNNGG 2 cut(s) 191, 441
BsmFI GGGAC 2 cut(s) 457, 531
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 2 cut(s) 49, 340
BspACI CCGC 4 cut(s) 26, 28, 198, 286
BspANI GGCC 1 cut(s) 424
BspFNI CGCG 2 cut(s) 28, 439
BspLI GGNNCC 2 cut(s) 196, 433
BspMI ACCTGC 2 cut(s) 59, 503
BspPI GGATC 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 26, 185
BssMI GATC 2 cut(s) 49, 340
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 4 cut(s) 90, 100, 205, 403
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 366
BstBAI YACGTR 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 409
BstDSI CCRYGG 1 cut(s) 26
BstFNI CGCG 2 cut(s) 28, 439
BstKTI GATC 2 cut(s) 52, 343
BstMBI GATC 2 cut(s) 49, 340
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 2 cut(s) 258, 266
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 76
BstUI CGCG 2 cut(s) 28, 439
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 1 cut(s) 424
BtgI CCRYGG 1 cut(s) 26
BtgZI GCGATG 1 cut(s) 6
BtsIMutI CAGTG 1 cut(s) 86
BveI ACCTGC 2 cut(s) 59, 503
Cac8I GCNNGC 1 cut(s) 256
Cfr42I CCGCGG 1 cut(s) 29
CseI GACGC 1 cut(s) 487
Csp6I GTAC 2 cut(s) 278, 506
CviAII CATG 4 cut(s) 131, 150, 255, 263
CviJI RGCY 5 cut(s) 195, 424, 531, 568, 575
CviKI_1 RGCY 5 cut(s) 195, 424, 531, 568, 575
CviQI GTAC 2 cut(s) 278, 506
DdeI CTNAG 1 cut(s) 409
DpnI GATC 2 cut(s) 51, 342
DpnII GATC 2 cut(s) 49, 340
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 185
Eco147I AGGCCT 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 185
EcoT22I ATGCAT 2 cut(s) 22, 256
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 4 cut(s) 134, 153, 258, 266
FaiI YATR 7 cut(s) 15, 84, 132, 151, 256, 264, 377
FaqI GGGAC 2 cut(s) 457, 531
FatI CATG 4 cut(s) 130, 149, 254, 262
FauI CCCGC 1 cut(s) 279
Fnu4HI GCNGC 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 26
FspBI CTAG 2 cut(s) 186, 338
GluI GCNGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 1 cut(s) 424
HgaI GACGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 4 cut(s) 134, 153, 258, 266
HinfI GANTC 3 cut(s) 62, 121, 391
Hpy166II GTNNAC 2 cut(s) 74, 364
Hpy188I TCNGA 2 cut(s) 106, 503
Hpy188III TCNNGA 2 cut(s) 236, 338
Hpy8I GTNNAC 2 cut(s) 74, 364
Hpy99I CGWCG 2 cut(s) 390, 503
HpyAV CCTTC 4 cut(s) 203, 289, 305, 545
HpyCH4III ACNGT 4 cut(s) 90, 100, 205, 403
HpyCH4IV ACGT 2 cut(s) 280, 366
HpyCH4V TGCA 7 cut(s) 20, 74, 86, 254, 258, 266, 492
HpyF3I CTNAG 1 cut(s) 409
HpySE526I ACGT 2 cut(s) 280, 366
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 4 cut(s) 134, 153, 258, 266
KspI CCGCGG 1 cut(s) 29
Kzo9I GATC 2 cut(s) 49, 340
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 7 cut(s) 54, 403, 411, 438, 508, 550, 557
LweI GCATC 1 cut(s) 29
MaeI CTAG 2 cut(s) 186, 338
MaeII ACGT 2 cut(s) 280, 366
MalI GATC 2 cut(s) 51, 342
MboI GATC 2 cut(s) 49, 340
MboII GAAGA 3 cut(s) 118, 251, 259
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 3 cut(s) 116, 145, 559
MlyI GAGTC 2 cut(s) 71, 400
MmeI TCCRAC 2 cut(s) 77, 481
MnlI CCTC 5 cut(s) 206, 414, 422, 434, 521
Mph1103I ATGCAT 2 cut(s) 22, 256
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 3 cut(s) 177, 305, 577
MspA1I CMGCKG 1 cut(s) 28
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 2 cut(s) 28, 439
NdeII GATC 2 cut(s) 49, 340
NlaIII CATG 4 cut(s) 134, 153, 258, 266
NlaIV GGNNCC 2 cut(s) 196, 433
NsiI ATGCAT 2 cut(s) 22, 256
NspI RCATGY 2 cut(s) 258, 266
PaeI GCATGC 1 cut(s) 258
PaqCI CACCTGC 2 cut(s) 59, 503
PceI AGGCCT 1 cut(s) 424
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 121
PflFI GACNNNGTC 1 cut(s) 390
PkrI GCNGC 1 cut(s) 27
PleI GAGTC 2 cut(s) 70, 399
PpsI GAGTC 2 cut(s) 70, 399
Ppu21I YACGTR 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 2 cut(s) 196, 433
PsuI RGATCY 1 cut(s) 49
PsyI GACNNNGTC 1 cut(s) 390
RsaI GTAC 2 cut(s) 279, 507
RsaNI GTAC 2 cut(s) 278, 506
SacII CCGCGG 1 cut(s) 29
SaqAI TTAA 3 cut(s) 177, 305, 577
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 2 cut(s) 49, 340
SchI GAGTC 2 cut(s) 71, 400
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 1 cut(s) 76
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 399
Sfr303I CCGCGG 1 cut(s) 29
SgrBI CCGCGG 1 cut(s) 29
SphI GCATGC 1 cut(s) 258
Sse9I AATT 3 cut(s) 116, 145, 559
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 4 cut(s) 26, 28, 198, 286
SspMI CTAG 2 cut(s) 186, 338
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 424
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 4 cut(s) 90, 100, 205, 403
TaiI ACGT 2 cut(s) 283, 369
TaqI TCGA 2 cut(s) 10, 135
TasI AATT 3 cut(s) 116, 145, 559
TatI WGTACW 1 cut(s) 505
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 3 cut(s) 177, 305, 577
Tru9I TTAA 3 cut(s) 177, 305, 577
TscAI CASTG 1 cut(s) 93
TspDTI ATGAA 3 cut(s) 129, 138, 260
TspGWI ACGGA 2 cut(s) 242, 403
TspRI CASTG 1 cut(s) 93
Tth111I GACNNNGTC 1 cut(s) 390
VneI GTGCAC 1 cut(s) 72
XapI RAATTY 1 cut(s) 559
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 2 cut(s) 258, 266
XmaJI CCTAGG 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 2 cut(s) 186, 338
ZraI GACGTC 1 cut(s) 367
Zsp2I ATGCAT 2 cut(s) 22, 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.