FvH4_4g10840

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008532
Physical Location & Seq
Reverse (-)
14539365 .. 14541590
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10840.t2

Sequence Viewer

Length: 513 bp
ATGGTGATGGTGCATCCATATGTGTCAGCCTCCACTCTAAGTCGAGCTCTGCCATCAGCAGCCTCTATCCTAAGTCGAGCTCTACCGACCTTATTTGCTGAACCAACGAAAAAATGGTTGGTGTGTGAGACTCAAAGCATGAAATTGTTAAATGATAGAGCAATTGCCAATGAATACAGAAACACAAAGAGACTTGTGTTTGGTGGTAGGAGGAGGTTCACTACTCGAGCTTCCCACGTCAATGATGCTGGCTCCATTGGCTCCCCTCTCATGCAGTCCATGGAGAACAAGATCAAGGAAGAGCTGAATGCAGAATCTGTTATTGTTAAAGATGCTAACGGGGATGGTCGGCATGTCAGCATTGATGTTATCTCTTCATCCTTTGAGGGAAAATCTGCTGTCAATAGGCAGAGGATGGTGTACAAAGCTATATGGGAGGAGCTTCAAAGCACAGTGCATGCAGTAGATCAGATGACTACTAAAACCCCAGAAGAAGCAGGTACTCAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

171

Amino Acids

18.85

Weight (kDa)

9.48

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 106 - 165 6.3e-14 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 488
AfaI GTAC 2 cut(s) 422, 502
AgsI TTSAA 1 cut(s) 446
AjiI CACGTC 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AluBI AGCT 6 cut(s) 47, 80, 230, 304, 428, 442
AluI AGCT 6 cut(s) 47, 80, 230, 304, 428, 442
Alw21I GWGCWC 2 cut(s) 49, 82
Alw26I GTCTC 2 cut(s) 122, 184
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 225
ApeKI GCWGC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 225
BanII GRGCYC 2 cut(s) 49, 82
Bbv12I GWGCWC 2 cut(s) 49, 82
BbvI GCAGC 1 cut(s) 71
BccI CCATC 3 cut(s) 61, 338, 409
BcoDI GTCTC 2 cut(s) 122, 184
BfuAI ACCTGC 1 cut(s) 488
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 238
BmiI GGNNCC 2 cut(s) 253, 262
BmsI GCATC 3 cut(s) 22, 235, 322
BsaJI CCNNGG 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 4 cut(s) 13, 349, 377, 420
BseRI GAGGAG 2 cut(s) 226, 452
BseXI GCAGC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 49, 82
BsiHKCI CYCGRG 1 cut(s) 225
BsmAI GTCTC 2 cut(s) 122, 184
BsmI GAATGC 1 cut(s) 313
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 49, 82
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 2 cut(s) 291, 466
Bsp19I CCATGG 1 cut(s) 279
BspLI GGNNCC 2 cut(s) 253, 262
BspMI ACCTGC 1 cut(s) 488
BspQI GCTCTTC 1 cut(s) 294
BsrGI TGTACA 1 cut(s) 420
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 2 cut(s) 291, 466
BssT1I CCWWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 2 cut(s) 294, 379
BstAUI TGTACA 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 250, 459
BstDEI CTNAG 2 cut(s) 38, 71
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 4 cut(s) 13, 349, 377, 420
BstKTI GATC 2 cut(s) 294, 469
BstMAI GTCTC 2 cut(s) 122, 184
BstMBI GATC 2 cut(s) 291, 466
BstMWI GCNNNNNNNGC 1 cut(s) 258
BstNSI RCATGY 2 cut(s) 356, 461
BstV1I GCAGC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 279
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 4 cut(s) 13, 349, 377, 420
BtsIMutI CAGTG 1 cut(s) 459
BveI ACCTGC 1 cut(s) 488
Cac8I GCNNGC 2 cut(s) 250, 459
CaiI CAGNNNCTG 1 cut(s) 317
Csp6I GTAC 2 cut(s) 421, 501
CviAII CATG 5 cut(s) 139, 271, 280, 353, 458
CviQI GTAC 2 cut(s) 421, 501
DdeI CTNAG 2 cut(s) 38, 71
DpnI GATC 2 cut(s) 293, 468
DpnII GATC 2 cut(s) 291, 466
Eam1104I CTCTTC 2 cut(s) 294, 379
EarI CTCTTC 2 cut(s) 294, 379
Ecl136II GAGCTC 2 cut(s) 47, 80
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 2 cut(s) 49, 82
Eco53kI GAGCTC 2 cut(s) 47, 80
Eco88I CYCGRG 1 cut(s) 225
EcoICRI GAGCTC 2 cut(s) 47, 80
EcoT14I CCWWGG 1 cut(s) 279
EcoT38I GRGCYC 2 cut(s) 49, 82
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 5 cut(s) 142, 274, 283, 356, 461
FaiI YATR 9 cut(s) 19, 21, 140, 272, 281, 354, 431, 433, 459
FatI CATG 5 cut(s) 138, 270, 279, 352, 457
FauNDI CATATG 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 60
FokI GGATG 3 cut(s) 356, 364, 427
FriOI GRGCYC 2 cut(s) 49, 82
Fsp4HI GCNGC 1 cut(s) 60
GluI GCNGC 1 cut(s) 60
Hin1II CATG 5 cut(s) 142, 274, 283, 356, 461
HinfI GANTC 2 cut(s) 130, 314
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 219, 421
Hpy188I TCNGA 1 cut(s) 471
Hpy8I GTNNAC 2 cut(s) 219, 421
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 5 cut(s) 13, 274, 311, 457, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 258
HpyF3I CTNAG 2 cut(s) 38, 71
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 5 cut(s) 142, 274, 283, 356, 461
Kzo9I GATC 2 cut(s) 291, 466
LguI GCTCTTC 1 cut(s) 294
LmnI GCTCC 3 cut(s) 257, 266, 439
LpnPI CCDG 3 cut(s) 234, 483, 501
Lsp1109I GCAGC 1 cut(s) 71
LweI GCATC 3 cut(s) 22, 235, 322
MaeII ACGT 1 cut(s) 237
MalI GATC 2 cut(s) 293, 468
MboI GATC 2 cut(s) 291, 466
MboII GAAGA 3 cut(s) 311, 366, 503
MfeI CAATTG 1 cut(s) 162
MhlI GDGCHC 2 cut(s) 49, 82
MluCI AATT 2 cut(s) 143, 162
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 8 cut(s) 40, 73, 204, 207, 276, 379, 405, 430
MseI TTAA 2 cut(s) 149, 327
MslI CAYNNNNRTG 2 cut(s) 18, 240
MunI CAATTG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 313
MwoI GCNNNNNNNGC 1 cut(s) 258
NcoI CCATGG 1 cut(s) 279
NdeI CATATG 1 cut(s) 19
NdeII GATC 2 cut(s) 291, 466
NlaIII CATG 5 cut(s) 142, 274, 283, 356, 461
NlaIV GGNNCC 2 cut(s) 253, 262
NspI RCATGY 2 cut(s) 356, 461
PaeI GCATGC 1 cut(s) 461
PaeR7I CTCGAG 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 294
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 1 cut(s) 61
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp124BI GAGCTC 2 cut(s) 49, 82
PspN4I GGNNCC 2 cut(s) 253, 262
PspXI VCTCGAGB 1 cut(s) 225
PstNI CAGNNNCTG 1 cut(s) 317
RsaI GTAC 2 cut(s) 422, 502
RsaNI GTAC 2 cut(s) 421, 501
RseI CAYNNNNRTG 2 cut(s) 18, 240
SacI GAGCTC 2 cut(s) 49, 82
SapI GCTCTTC 1 cut(s) 294
SaqAI TTAA 2 cut(s) 149, 327
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 2 cut(s) 291, 466
SchI GAGTC 1 cut(s) 124
SduI GDGCHC 2 cut(s) 49, 82
SfaNI GCATC 3 cut(s) 22, 235, 322
Sfr274I CTCGAG 1 cut(s) 225
SlaI CTCGAG 1 cut(s) 225
SmiMI CAYNNNNRTG 2 cut(s) 18, 240
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
SphI GCATGC 1 cut(s) 461
Sse9I AATT 2 cut(s) 143, 162
SstI GAGCTC 2 cut(s) 49, 82
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 454
TaiI ACGT 1 cut(s) 240
TaqI TCGA 3 cut(s) 43, 76, 226
TasI AATT 2 cut(s) 143, 162
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 2 cut(s) 149, 327
Tru9I TTAA 2 cut(s) 149, 327
TscAI CASTG 1 cut(s) 459
TseI GCWGC 1 cut(s) 59
TspDTI ATGAA 3 cut(s) 155, 186, 366
TspRI CASTG 1 cut(s) 459
XceI RCATGY 2 cut(s) 356, 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.