pycom08g04140

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008532
Physical Location & Seq
Reverse (-)
3179922 .. 3180398
477 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04140.1

Sequence Viewer

Length: 477 bp
ATGGCGGTGAAGTCAATGATGGTGCGTCCATACGTGTTATCAGCCTCCTGCGCCCTTAGTCGAGCTCTGCCATCCTTATTTTCTCAACCAAGGAAAACACAGTTGGTATTGCTTCACCATAGAGCAATCAGCAATGAAAACAGGAGGAATATGAGACTGGAATATGGTGGTGTGAGGAGATTCAGTCCTCGATCCTCAAGTGTGGGCAATGCTGGCTCCATCGACTCCCCCCTCATAGAGTCCATGAAGAAAAAGATCAAGGAGGAGCTAAATGCTGAATCCGTTAGCGTAGAAGATCTGAGTGGGGATGGCCGACATGTGGTCATTGATGTCATCTCCTCGTCATTCGAGGGACAGTCGGCTGTAAATAGGCAGAGGATGGTGTACAAAGCCATATGGGAGGAGCTTCAGAACACAGTTCATGCAGTTGATCAGATGACTACCAAAACCCCGGATGAAGTTTCTGGTAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

159

Amino Acids

17.6

Weight (kDa)

9.67

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 101 - 153 5e-13 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 186
AcoI YGGCCR 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 392
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 1 cut(s) 319
AflIII ACRYGT 2 cut(s) 33, 316
AhdI GACNNNNNGTC 1 cut(s) 320
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AluBI AGCT 3 cut(s) 65, 268, 406
AluI AGCT 3 cut(s) 65, 268, 406
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 1 cut(s) 186
AoxI GGCC 1 cut(s) 310
AspLEI GCGC 1 cut(s) 53
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 2 cut(s) 19, 107
BanII GRGCYC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 67
BccI CCATC 5 cut(s) 13, 79, 227, 302, 373
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 1 cut(s) 452
BcoDI GTCTC 1 cut(s) 148
BglII AGATCT 1 cut(s) 295
Bme1390I CCNGG 1 cut(s) 452
BmeRI GACNNNNNGTC 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 181
BpuMI CCSGG 1 cut(s) 452
BsaAI YACGTR 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 89, 450
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 162
Bse3DI GCAATG 2 cut(s) 139, 214
BseDI CCNNGG 2 cut(s) 89, 450
BseGI GGATG 4 cut(s) 71, 313, 384, 460
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 2 cut(s) 139, 214
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 4 cut(s) 190, 278, 328, 416
BshFI GGCC 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 67
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 4 cut(s) 191, 255, 295, 430
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 217
BspPI GGATC 1 cut(s) 186
BsrDI GCAATG 2 cut(s) 139, 214
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 2 cut(s) 89, 450
BssMI GATC 4 cut(s) 191, 255, 295, 430
BssT1I CCWWGG 1 cut(s) 89
Bst4CI ACNGT 3 cut(s) 102, 357, 418
BstAUI TGTACA 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 2 cut(s) 56, 299
BstF5I GGATG 4 cut(s) 71, 313, 384, 460
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 4 cut(s) 194, 258, 298, 433
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 4 cut(s) 191, 255, 295, 430
BstMWI GCNNNNNNNGC 2 cut(s) 50, 213
BstNSI RCATGY 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 450
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 1 cut(s) 312
BtsCI GGATG 4 cut(s) 71, 313, 384, 460
Cac8I GCNNGC 1 cut(s) 214
CfoI GCGC 1 cut(s) 53
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 385
CviAII CATG 3 cut(s) 244, 317, 422
CviJI RGCY 8 cut(s) 44, 65, 216, 268, 312, 362, 392, 406
CviKI_1 RGCY 8 cut(s) 44, 65, 216, 268, 312, 362, 392, 406
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 56, 299
DpnI GATC 4 cut(s) 193, 257, 297, 432
DpnII GATC 4 cut(s) 191, 255, 295, 430
DriI GACNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 1 cut(s) 310
Eam1105I GACNNNNNGTC 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 3 cut(s) 247, 320, 425
FaqI GGGAC 1 cut(s) 366
FatI CATG 3 cut(s) 243, 316, 421
FauNDI CATATG 1 cut(s) 395
FbaI TGATCA 1 cut(s) 430
FokI GGATG 4 cut(s) 58, 320, 391, 467
FriOI GRGCYC 1 cut(s) 67
GlaI GCGC 1 cut(s) 52
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 452
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 53
Hin1II CATG 3 cut(s) 247, 320, 425
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 4 cut(s) 180, 224, 239, 278
HpaII CCGG 1 cut(s) 452
HphI GGTGA 2 cut(s) 19, 107
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 3 cut(s) 300, 411, 435
Hpy8I GTNNAC 1 cut(s) 385
HpyCH4III ACNGT 3 cut(s) 102, 357, 418
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 213
HpyF3I CTNAG 2 cut(s) 56, 299
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 3 cut(s) 247, 320, 425
HspAI GCGC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 4 cut(s) 191, 255, 295, 430
LmnI GCTCC 3 cut(s) 221, 265, 403
LpnPI CCDG 6 cut(s) 61, 127, 143, 198, 450, 465
MaeII ACGT 1 cut(s) 33
MalI GATC 4 cut(s) 193, 257, 297, 432
MboI GATC 4 cut(s) 191, 255, 295, 430
MboII GAAGA 2 cut(s) 259, 305
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 67
MlyI GAGTC 2 cut(s) 218, 248
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 50, 213
NciI CCSGG 1 cut(s) 452
NdeI CATATG 1 cut(s) 395
NdeII GATC 4 cut(s) 191, 255, 295, 430
NlaIII CATG 3 cut(s) 247, 320, 425
NlaIV GGNNCC 1 cut(s) 217
NspI RCATGY 1 cut(s) 320
PciI ACATGT 1 cut(s) 316
PfeI GAWTC 2 cut(s) 180, 278
PleI GAGTC 2 cut(s) 218, 247
PpsI GAGTC 2 cut(s) 218, 247
Ppu21I YACGTR 1 cut(s) 34
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 217
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SacI GAGCTC 1 cut(s) 67
Sau3AI GATC 4 cut(s) 191, 255, 295, 430
SchI GAGTC 2 cut(s) 218, 248
ScrFI CCNGG 1 cut(s) 452
SduI GDGCHC 1 cut(s) 67
SetI ASST 4 cut(s) 36, 67, 270, 408
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
SsiI CCGC 1 cut(s) 5
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 3 cut(s) 102, 357, 418
TaiI ACGT 1 cut(s) 36
TaqI TCGA 4 cut(s) 61, 190, 222, 348
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 2 cut(s) 180, 278
TspDTI ATGAA 4 cut(s) 150, 260, 410, 471
TspGWI ACGGA 1 cut(s) 271
XceI RCATGY 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.