MD08G1049500.v1.1

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008532
Physical Location & Seq
Reverse (-)
3873082 .. 3874920
1839 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1049500.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGCGGTGAAGTCAATGATGGTACGTCCATATGTGTTATCAGCCTCCTGCTTCCTTAGTCGAGCTCTACCATCCTTATTTTCTCAACCAAGGCAAACACAGTTGGTATTGCTGCACCATAGAGCAATCAGCAATGAAAACAGGAGGAATATGAGACAGGAATATGGTGGTGTCAGGAGATTCAGTCCTCGATCCTCGAGCGTGGGCAATGCTGGCTCCATCGACTCCCCCCTCATAGAGTCCATGAAGAAAAAGATCAAGGAGGAGCTAAATGCTGAATCTGTTAGCGTAGAAGATCTGAGTGGGGATGGCCGACATGTGGTCATTGATGTTGTCTCCTCGTCGTTTGAGGGACAGTCGGCTGTAAATAGGCAGAGGATGGTGTACAAAGCCATATGGGAGGAGCTTCAGAACACAGTTCACGCAGTTGATCAGATGACGACCAAAACCCCGGATGAAGTTTCTGGTAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

159

Amino Acids

17.68

Weight (kDa)

9.56

Isoelectric Point (pI)

56.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 101 - 153 1.2e-13 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 186
AcoI YGGCCR 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 392
AfaI GTAC 2 cut(s) 24, 386
AfiI CCNNNNNNNGG 1 cut(s) 319
AflIII ACRYGT 1 cut(s) 316
AhdI GACNNNNNGTC 1 cut(s) 320
AluBI AGCT 3 cut(s) 65, 268, 406
AluI AGCT 3 cut(s) 65, 268, 406
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 2 cut(s) 148, 340
AlwI GGATC 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 1 cut(s) 112
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 196
BanII GRGCYC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 99
BccI CCATC 5 cut(s) 13, 79, 227, 302, 373
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 1 cut(s) 452
BcoDI GTCTC 2 cut(s) 148, 340
BglII AGATCT 1 cut(s) 295
BisI GCNGC 1 cut(s) 113
BlsI GCNGC 1 cut(s) 114
Bme1390I CCNGG 1 cut(s) 452
BmeRI GACNNNNNGTC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 89, 450
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse3DI GCAATG 2 cut(s) 139, 214
BseDI CCNNGG 2 cut(s) 89, 450
BseGI GGATG 4 cut(s) 71, 313, 384, 460
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 2 cut(s) 139, 214
BseMII CTCAG 1 cut(s) 290
BseRI GAGGAG 3 cut(s) 278, 328, 416
BseXI GCAGC 1 cut(s) 99
BsgI GTGCAG 1 cut(s) 98
BshFI GGCC 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 148, 340
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 4 cut(s) 191, 255, 295, 430
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 217
BspPI GGATC 1 cut(s) 186
BsrDI GCAATG 2 cut(s) 139, 214
BsrGI TGTACA 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 89, 450
BssMI GATC 4 cut(s) 191, 255, 295, 430
BssT1I CCWWGG 1 cut(s) 89
Bst4CI ACNGT 3 cut(s) 102, 357, 418
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 2 cut(s) 56, 299
BstF5I GGATG 4 cut(s) 71, 313, 384, 460
BstKTI GATC 4 cut(s) 194, 258, 298, 433
BstMAI GTCTC 2 cut(s) 148, 340
BstMBI GATC 4 cut(s) 191, 255, 295, 430
BstMWI GCNNNNNNNGC 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 1 cut(s) 312
BtsCI GGATG 4 cut(s) 71, 313, 384, 460
Cac8I GCNNGC 1 cut(s) 214
Csp6I GTAC 2 cut(s) 23, 385
CviAII CATG 2 cut(s) 244, 317
CviJI RGCY 8 cut(s) 44, 65, 216, 268, 312, 362, 392, 406
CviKI_1 RGCY 8 cut(s) 44, 65, 216, 268, 312, 362, 392, 406
CviQI GTAC 2 cut(s) 23, 385
DdeI CTNAG 2 cut(s) 56, 299
DpnI GATC 4 cut(s) 193, 257, 297, 432
DpnII GATC 4 cut(s) 191, 255, 295, 430
DriI GACNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 1 cut(s) 310
Eam1105I GACNNNNNGTC 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 2 cut(s) 247, 320
FaqI GGGAC 1 cut(s) 366
FatI CATG 2 cut(s) 243, 316
FauNDI CATATG 2 cut(s) 31, 395
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 1 cut(s) 113
FokI GGATG 4 cut(s) 58, 320, 391, 467
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 113
GluI GCNGC 1 cut(s) 113
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 452
Hin1II CATG 2 cut(s) 247, 320
HinfI GANTC 4 cut(s) 180, 224, 239, 278
HpaII CCGG 1 cut(s) 452
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 385, 421
Hpy188I TCNGA 3 cut(s) 300, 411, 435
Hpy188III TCNNGA 1 cut(s) 175
Hpy8I GTNNAC 2 cut(s) 385, 421
Hpy99I CGWCG 1 cut(s) 346
HpyCH4III ACNGT 3 cut(s) 102, 357, 418
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 1 cut(s) 213
HpyF3I CTNAG 2 cut(s) 56, 299
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 2 cut(s) 247, 320
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 4 cut(s) 191, 255, 295, 430
LmnI GCTCC 3 cut(s) 221, 265, 403
LpnPI CCDG 7 cut(s) 61, 127, 143, 160, 198, 450, 465
Lsp1109I GCAGC 1 cut(s) 99
MaeII ACGT 1 cut(s) 25
MalI GATC 4 cut(s) 193, 257, 297, 432
MboI GATC 4 cut(s) 191, 255, 295, 430
MboII GAAGA 2 cut(s) 259, 305
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 67
MlyI GAGTC 2 cut(s) 218, 248
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 452
MwoI GCNNNNNNNGC 1 cut(s) 213
NciI CCSGG 1 cut(s) 452
NdeI CATATG 2 cut(s) 31, 395
NdeII GATC 4 cut(s) 191, 255, 295, 430
NlaIII CATG 2 cut(s) 247, 320
NlaIV GGNNCC 1 cut(s) 217
NspI RCATGY 1 cut(s) 320
PaeR7I CTCGAG 1 cut(s) 196
PciI ACATGT 1 cut(s) 316
PfeI GAWTC 2 cut(s) 180, 278
PkrI GCNGC 1 cut(s) 114
PleI GAGTC 2 cut(s) 218, 247
PpsI GAGTC 2 cut(s) 218, 247
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 217
PspXI VCTCGAGB 1 cut(s) 196
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 2 cut(s) 24, 386
RsaNI GTAC 2 cut(s) 23, 385
SacI GAGCTC 1 cut(s) 67
SatI GCNGC 1 cut(s) 113
Sau3AI GATC 4 cut(s) 191, 255, 295, 430
SchI GAGTC 2 cut(s) 218, 248
ScrFI CCNGG 1 cut(s) 452
SduI GDGCHC 1 cut(s) 67
SetI ASST 4 cut(s) 28, 67, 270, 408
Sfr274I CTCGAG 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 196
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
SsiI CCGC 1 cut(s) 5
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 3 cut(s) 102, 357, 418
TaiI ACGT 1 cut(s) 28
TaqI TCGA 4 cut(s) 61, 190, 197, 222
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 2 cut(s) 180, 278
TseI GCWGC 1 cut(s) 112
TspDTI ATGAA 3 cut(s) 150, 260, 471
XceI RCATGY 1 cut(s) 320
XhoI CTCGAG 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.