Rw4G011390

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008532
Physical Location & Seq
Forward (+)
26932512 .. 26934556
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011390.1

Sequence Viewer

Length: 525 bp
ATGGTGGCTGGGATGGTGATGGTGCGTCCGTATGTGTCAGCCTCAATGCTAAGTCGAGCTCTGCCATCAGCAGCTTCTGCACTAAGTCGAGCTCTACCAACCTTATTTACTGAACCAAGGAATAATTGGTTAGTGTGTGAGACTCAGAGCATGAAATTGTTAAATGATAGAGCAACCGCCAACAAACACAGAAACTCAAGGAGACTGGTGTTTGGTGGTGGGAGGAGGTTCACTACTCGAGCTTCCCACGTCAATGATGCTGGCTACATTGGCTCGCCTCTCATGCAGTCCATGGAGAACAAGATCAAGGAACAGCTGAATGCAGAATCTGTTATTGTTAAAGATGCTAACGGGGATGGTCGGCATGTCAGCATTGATGTTGTCTCTTCATCCTTTGAGGGCCAATCTGCTGTGAATAGGCAGAGGATGGTGTACAAAGCCATATGGGAGGAGCTTCAAAACACAGTGCATGCAGTAGATCAGATGACTACTAAAACCCCAGATGAAGCAGCAACACAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

174

Amino Acids

19.24

Weight (kDa)

9.77

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 116 - 169 3.8e-14 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 177
AfaI GTAC 1 cut(s) 434
AgsI TTSAA 1 cut(s) 458
AjiI CACGTC 1 cut(s) 250
AleI CACNNNNGTG 1 cut(s) 520
AluBI AGCT 6 cut(s) 59, 74, 92, 242, 316, 454
AluI AGCT 6 cut(s) 59, 74, 92, 242, 316, 454
Alw21I GWGCWC 2 cut(s) 61, 94
Alw26I GTCTC 3 cut(s) 134, 196, 388
AlwNI CAGNNNCTG 2 cut(s) 77, 329
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 1 cut(s) 400
ApeKI GCWGC 2 cut(s) 71, 509
AspS9I GGNCC 1 cut(s) 400
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 237
BanII GRGCYC 2 cut(s) 61, 94
Bbv12I GWGCWC 2 cut(s) 61, 94
BbvI GCAGC 2 cut(s) 83, 521
BccI CCATC 5 cut(s) 7, 13, 73, 350, 421
BcoDI GTCTC 3 cut(s) 134, 196, 388
BisI GCNGC 2 cut(s) 72, 510
BlsI GCNGC 2 cut(s) 73, 511
BmeT110I CYCGRG 1 cut(s) 237
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 400
BmsI GCATC 2 cut(s) 247, 334
BpuEI CTTGAG 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 116, 291
Bse1I ACTGG 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 116, 291
BseGI GGATG 4 cut(s) 18, 361, 389, 432
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 210
BseRI GAGGAG 2 cut(s) 238, 464
BseXI GCAGC 2 cut(s) 83, 521
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 61, 94
BsiHKCI CYCGRG 1 cut(s) 237
BsmAI GTCTC 3 cut(s) 134, 196, 388
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 61, 94
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 2 cut(s) 303, 478
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 1 cut(s) 177
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 1 cut(s) 210
BssECI CCNNGG 2 cut(s) 116, 291
BssMI GATC 2 cut(s) 303, 478
BssT1I CCWWGG 2 cut(s) 116, 291
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 3 cut(s) 262, 275, 471
BstDEI CTNAG 3 cut(s) 50, 83, 144
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 4 cut(s) 18, 361, 389, 432
BstKTI GATC 2 cut(s) 306, 481
BstMAI GTCTC 3 cut(s) 134, 196, 388
BstMBI GATC 2 cut(s) 303, 478
BstMWI GCNNNNNNNGC 3 cut(s) 77, 270, 283
BstNSI RCATGY 2 cut(s) 368, 473
BstV1I GCAGC 2 cut(s) 83, 521
BsuRI GGCC 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 291
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 4 cut(s) 18, 361, 389, 432
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 3 cut(s) 262, 275, 471
CaiI CAGNNNCTG 2 cut(s) 77, 329
Cfr13I GGNCC 1 cut(s) 400
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 433
CviAII CATG 5 cut(s) 151, 283, 292, 365, 470
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 3 cut(s) 50, 83, 144
DpnI GATC 2 cut(s) 305, 480
DpnII GATC 2 cut(s) 303, 478
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Ecl136II GAGCTC 2 cut(s) 59, 92
Eco130I CCWWGG 2 cut(s) 116, 291
Eco24I GRGCYC 2 cut(s) 61, 94
Eco53kI GAGCTC 2 cut(s) 59, 92
Eco88I CYCGRG 1 cut(s) 237
EcoICRI GAGCTC 2 cut(s) 59, 92
EcoT14I CCWWGG 2 cut(s) 116, 291
EcoT38I GRGCYC 2 cut(s) 61, 94
ErhI CCWWGG 2 cut(s) 116, 291
FaeI CATG 5 cut(s) 154, 286, 295, 368, 473
FaiI YATR 8 cut(s) 33, 152, 284, 293, 366, 443, 445, 471
FatI CATG 5 cut(s) 150, 282, 291, 364, 469
FauNDI CATATG 1 cut(s) 443
Fnu4HI GCNGC 2 cut(s) 72, 510
FokI GGATG 4 cut(s) 25, 368, 376, 439
FriOI GRGCYC 2 cut(s) 61, 94
Fsp4HI GCNGC 2 cut(s) 72, 510
GluI GCNGC 2 cut(s) 72, 510
GsaI CCCAGC 1 cut(s) 12
HaeIII GGCC 1 cut(s) 402
HgaI GACGC 1 cut(s) 14
Hin1II CATG 5 cut(s) 154, 286, 295, 368, 473
HinfI GANTC 2 cut(s) 142, 326
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 231, 433
Hpy188I TCNGA 2 cut(s) 147, 483
Hpy8I GTNNAC 2 cut(s) 231, 433
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 5 cut(s) 80, 286, 323, 469, 473
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 270, 283
HpyF3I CTNAG 3 cut(s) 50, 83, 144
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 5 cut(s) 154, 286, 295, 368, 473
Kzo9I GATC 2 cut(s) 303, 478
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 3 cut(s) 191, 246, 513
Lsp1109I GCAGC 2 cut(s) 83, 521
LweI GCATC 2 cut(s) 247, 334
MaeII ACGT 1 cut(s) 249
MalI GATC 2 cut(s) 305, 480
MboI GATC 2 cut(s) 303, 478
MboII GAAGA 1 cut(s) 378
MhlI GDGCHC 2 cut(s) 61, 94
MluCI AATT 2 cut(s) 124, 155
MlyI GAGTC 1 cut(s) 136
MnlI CCTC 7 cut(s) 52, 216, 219, 288, 391, 417, 442
MseI TTAA 2 cut(s) 161, 339
MslI CAYNNNNRTG 2 cut(s) 252, 520
MspA1I CMGCKG 1 cut(s) 316
Mva1269I GAATGC 1 cut(s) 325
MwoI GCNNNNNNNGC 3 cut(s) 77, 270, 283
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 443
NdeII GATC 2 cut(s) 303, 478
NlaIII CATG 5 cut(s) 154, 286, 295, 368, 473
NspI RCATGY 2 cut(s) 368, 473
OliI CACNNNNGTG 1 cut(s) 520
PaeI GCATGC 1 cut(s) 473
PaeR7I CTCGAG 1 cut(s) 237
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 73, 511
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Psp124BI GAGCTC 2 cut(s) 61, 94
PspFI CCCAGC 1 cut(s) 8
PspPI GGNCC 1 cut(s) 400
PspXI VCTCGAGB 1 cut(s) 237
PstNI CAGNNNCTG 2 cut(s) 77, 329
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 2 cut(s) 252, 520
SacI GAGCTC 2 cut(s) 61, 94
SaqAI TTAA 2 cut(s) 161, 339
SatI GCNGC 2 cut(s) 72, 510
Sau3AI GATC 2 cut(s) 303, 478
Sau96I GGNCC 1 cut(s) 400
SchI GAGTC 1 cut(s) 136
SduI GDGCHC 2 cut(s) 61, 94
SetI ASST 9 cut(s) 61, 76, 94, 104, 230, 244, 252, 318, 456
SfaNI GCATC 2 cut(s) 247, 334
Sfr274I CTCGAG 1 cut(s) 237
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 252, 520
SmlI CTYRAG 2 cut(s) 196, 237
SmoI CTYRAG 2 cut(s) 196, 237
SphI GCATGC 1 cut(s) 473
Sse9I AATT 2 cut(s) 124, 155
SsiI CCGC 1 cut(s) 177
SstI GAGCTC 2 cut(s) 61, 94
StyI CCWWGG 2 cut(s) 116, 291
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 55, 88, 238
TasI AATT 2 cut(s) 124, 155
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 161, 339
Tru9I TTAA 2 cut(s) 161, 339
TscAI CASTG 1 cut(s) 471
TseI GCWGC 2 cut(s) 71, 509
TspDTI ATGAA 3 cut(s) 167, 378, 519
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 1 cut(s) 471
XceI RCATGY 2 cut(s) 368, 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 123
XhoI CTCGAG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.