pycom15g04190

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008532
Physical Location & Seq
Forward (+)
2526789 .. 2527268
480 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04190.1

Sequence Viewer

Length: 480 bp
ATGGTGCGTCCATACGTGTTATCAGCCTCCTGCGCCCTTACACGAGCTCTGCCATCTTTATTTTCTCAACCAAGGAAAACACTGTTGGTACTTAAGAGCCAGAGCATCAGATTGTTGCACCATAGAGCAATTGGCAATGAAAACAGGAGCAATATGAGATTGGAATACGGTGGTGTGAGAAGATTCAGTCCTCGAGCTTCAAGTGTCGGAAATGCTGGATCCATTGACTCCCCACTAATAGAATCCATGAAGAAAAAGATCAAGGAGGAGCTAAATGCGGAATCCGTTAGTGTAGAAGATATGAACGGGGATGGCCGGCATGTGGTCATTGATGTCGTCTCCTCATCATTTGAGGGACAGTCGGCTGTCAATAGGCAGAGGATGGTGTACAAAGCCATATGGGAGGAGCTTCAGAACACAGTTCATGCAGTCGATCAGATGACTACCAAAACCCCGGATGAAGTTTCTGGTAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

160

Amino Acids

17.68

Weight (kDa)

9.67

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 93 - 154 6.5e-14 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 278
AclWI GGATC 2 cut(s) 213, 226
AcoI YGGCCR 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 395
AfaI GTAC 2 cut(s) 90, 389
AfiI CCNNNNNNNGG 1 cut(s) 322
AflII CTTAAG 1 cut(s) 92
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 1 cut(s) 201
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AluBI AGCT 4 cut(s) 47, 197, 271, 409
AluI AGCT 4 cut(s) 47, 197, 271, 409
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 343
AlwI GGATC 2 cut(s) 213, 226
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 1 cut(s) 313
AspLEI GCGC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 192
BamHI GGATCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 49
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 49
BccI CCATC 3 cut(s) 61, 305, 376
BcnI CCSGG 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 343
BfrI CTTAAG 1 cut(s) 92
Bme1390I CCNGG 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 1 cut(s) 455
BmsI GCATC 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 455
BsaAI YACGTR 1 cut(s) 16
BsaJI CCNNGG 2 cut(s) 71, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse118I RCCGGY 1 cut(s) 315
Bse3DI GCAATG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 71, 453
BseGI GGATG 3 cut(s) 316, 387, 463
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMI GCAATG 1 cut(s) 142
BseRI GAGGAG 3 cut(s) 281, 331, 419
BshFI GGCC 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 2 cut(s) 316, 455
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 1 cut(s) 343
BsmBI CGTCTC 1 cut(s) 343
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 1 cut(s) 315
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 49
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 3 cut(s) 218, 258, 433
BspACI CCGC 1 cut(s) 278
BspANI GGCC 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 220
BspPI GGATC 2 cut(s) 213, 226
BspTI CTTAAG 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 315
BsrGI TGTACA 1 cut(s) 387
BssAI RCCGGY 1 cut(s) 315
BssECI CCNNGG 2 cut(s) 71, 453
BssMI GATC 3 cut(s) 218, 258, 433
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 71
Bst2BI CACGAG 1 cut(s) 42
Bst4CI ACNGT 4 cut(s) 84, 170, 360, 421
BstAFI CTTAAG 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 387
BstBAI YACGTR 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 317
BstF5I GGATG 3 cut(s) 316, 387, 463
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 3 cut(s) 221, 261, 436
BstMAI GTCTC 1 cut(s) 343
BstMBI GATC 3 cut(s) 218, 258, 433
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BsuRI GGCC 1 cut(s) 315
BtsCI GGATG 3 cut(s) 316, 387, 463
BtsIMutI CAGTG 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 317
CfoI GCGC 1 cut(s) 35
Cfr10I RCCGGY 1 cut(s) 315
Csp6I GTAC 2 cut(s) 89, 388
CviAII CATG 3 cut(s) 247, 320, 425
CviJI RGCY 9 cut(s) 26, 47, 99, 197, 271, 315, 365, 395, 409
CviKI_1 RGCY 9 cut(s) 26, 47, 99, 197, 271, 315, 365, 395, 409
CviQI GTAC 2 cut(s) 89, 388
DpnI GATC 3 cut(s) 220, 260, 435
DpnII GATC 3 cut(s) 218, 258, 433
EaeI YGGCCR 1 cut(s) 313
Ecl136II GAGCTC 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 395
Eco88I CYCGRG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 47
EcoT14I CCWWGG 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 3 cut(s) 250, 323, 428
FaiI YATR 9 cut(s) 13, 123, 155, 248, 302, 321, 398, 400, 426
FaqI GGGAC 1 cut(s) 369
FatI CATG 3 cut(s) 246, 319, 424
FauNDI CATATG 1 cut(s) 398
FokI GGATG 3 cut(s) 323, 394, 470
FriOI GRGCYC 1 cut(s) 49
GlaI GCGC 1 cut(s) 34
HaeIII GGCC 1 cut(s) 315
HapII CCGG 2 cut(s) 316, 455
HhaI GCGC 1 cut(s) 35
Hin1II CATG 3 cut(s) 250, 323, 428
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 4 cut(s) 183, 227, 242, 281
HpaII CCGG 2 cut(s) 316, 455
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 4 cut(s) 110, 209, 414, 438
Hpy8I GTNNAC 1 cut(s) 388
HpyCH4III ACNGT 4 cut(s) 84, 170, 360, 421
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 2 cut(s) 118, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 3 cut(s) 250, 323, 428
HspAI GCGC 1 cut(s) 33
KroI GCCGGC 1 cut(s) 315
KroNI GCCGGC 1 cut(s) 317
Kzo9I GATC 3 cut(s) 218, 258, 433
LmnI GCTCC 3 cut(s) 147, 268, 406
LpnPI CCDG 7 cut(s) 43, 113, 130, 201, 329, 453, 468
LweI GCATC 1 cut(s) 114
MaeII ACGT 1 cut(s) 15
MalI GATC 3 cut(s) 220, 260, 435
MboI GATC 3 cut(s) 218, 258, 433
MboII GAAGA 3 cut(s) 192, 262, 308
MfeI CAATTG 1 cut(s) 129
MflI RGATCY 1 cut(s) 218
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 1 cut(s) 129
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 7 cut(s) 37, 201, 259, 346, 352, 372, 397
MroNI GCCGGC 1 cut(s) 315
MseI TTAA 1 cut(s) 93
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 2 cut(s) 316, 455
MspR9I CCNGG 1 cut(s) 455
MunI CAATTG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 32
NaeI GCCGGC 1 cut(s) 317
NciI CCSGG 1 cut(s) 455
NdeI CATATG 1 cut(s) 398
NdeII GATC 3 cut(s) 218, 258, 433
NgoMIV GCCGGC 1 cut(s) 315
NlaIII CATG 3 cut(s) 250, 323, 428
NlaIV GGNNCC 1 cut(s) 220
NspI RCATGY 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 192
PdiI GCCGGC 1 cut(s) 317
PfeI GAWTC 3 cut(s) 183, 242, 281
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Ppu21I YACGTR 1 cut(s) 16
Psp124BI GAGCTC 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 220
PspXI VCTCGAGB 1 cut(s) 192
PsuI RGATCY 1 cut(s) 218
RsaI GTAC 2 cut(s) 90, 389
RsaNI GTAC 2 cut(s) 89, 388
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 93
Sau3AI GATC 3 cut(s) 218, 258, 433
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 455
SduI GDGCHC 1 cut(s) 49
SetI ASST 5 cut(s) 18, 49, 199, 273, 411
SfaNI GCATC 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 192
SmlI CTYRAG 2 cut(s) 92, 192
SmoI CTYRAG 2 cut(s) 92, 192
Sse9I AATT 1 cut(s) 129
SsiI CCGC 1 cut(s) 278
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 1 cut(s) 453
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 4 cut(s) 84, 170, 360, 421
TaiI ACGT 1 cut(s) 18
TaqI TCGA 2 cut(s) 193, 432
TasI AATT 1 cut(s) 129
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 3 cut(s) 183, 242, 281
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 87
TspDTI ATGAA 5 cut(s) 153, 263, 317, 413, 474
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 1 cut(s) 87
Vha464I CTTAAG 1 cut(s) 92
XceI RCATGY 1 cut(s) 323
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XhoI CTCGAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.