Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0008532

Physical Location & Seq

Genomic Coordinates Forward (+)

2534343 .. 2537314

Gene Structure 2972 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g04220.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2250 bp

ATGATAAAAAAATATAATGAACAAAACCATAACTTGAGGATGGTGAGAAAAAAATATTTCTTTAAAATTTTCAATACTGCACTGAAATGGAAGTGGTACTTGAAGAACGTGATTAACAACAACAATTTCTTTGACTATTTCACATTAAATTTAATACAAAAACAAAGTATAACAAATACGTTAGAAATTTATTTTACACACATCTTTATAATTTATATTGTTGACATAAGATGTCCTGATAACAGTATATCTATACATCATTCTCCTGCTCTAGGGTTTTGTTTCATGCTTGGGCCCATATCTGAAGCCCAGATATGTGCATCGAATGAAATTGACATGAAAACATTCATTGGATTAGTGATAAAATTTGGACCCTTTAGTTTAAGAAGCGCCTTTAGTTGGTTATCTAAACACAGTTCCAAACAAATTCTTAGGCGCTTGTTTGGGAATGCTTCTGTAGGAAGCTTGCTCATAAGCGCTTATGCTAGAAGACATTGCATTATAAATATGTCGAGTTTCTTCAAAAAGAGATTATGGGTAAAAGCGCTTTTATATTTTTCTGCCAAACGCAGTTCGAAGCGCTTTCAGAAGCAGTCCCAATATGGCCTAATATTGACTGTTATTCGAACTTGTTTGCTGTATCATTTATTCTTTCCAATTTTAATTCGTACCATCATATTCGTGCAGGTGCTCAATTCTTGCATTAGAATGTGTAGACAGGGAAGAATGGCGGCCAAGTCAATCATGGTGCGTCCATACGTGTTATCAGCCTCCTGCGCCCTTTATCGAGCTCTGCCATCCTTATTTTCTCAACCAAGGAAAACTCTGTTGGTATTTAAGAGCCAGAGCATCAGATTGTTGCACCATAAAGCAATTGGCAATGAAAACAGGAGCAGTATCAGATTGGAATACGGTGGTGTGAGAAGATTCAGTCCTCGAGCTTCAAGCGTCGGAAATGCTGGATCCATTGACTCCCCACTAATAGAATCCATGAAGAAAGAGATAAAGGAGGAGCTAAATGCGGAATCCGTTAGTGTAGAAGATATGAACGGGGATGGCCGGCATGTGGTCATTGATGTCGTCTCCTCGTCATTTGAGGGACAGTCGGCTGTAAATAGGCAGAGGATGGTATACAAAGCCATATGGGAGGAGCTTCAGAACATAGTTCATGCAGTTGATCAGATGACTACCAAAACCCCGGATGAAGTTTCTGGTAGGAACAACAAATGCGTCCCGAGTTCAAATCATCCCCTTCCTTTAGTCTATTGTAAAACAATTGTTGGATTTGTTACAACAATTAAATTAGGCTACAAAGCCAAATATTATTGTCAACAGTTGTTCGATACTAGAAGAGGGAGAATCGGTATGGTAATCCATGCAATCACCATTGATGAAGCTTTGATTATAGTCGTCGATGCAGGCTATGACACATTTATATTTCGAGCATTTTTTAACGGGTCGGAGCATTTGAGCATTTTCGTTCGTCTCCTTGAAGTGCACCTGATGACGAAACAATTTAGGAAATGCAAAAATGTAGAGAAAGAAACCGATATTAAGAAGAGATTAGATGGAAGAAACACCGGCGCTTCATCTGTCTTCGAGAGCAAATGCTATATTAAGCTCTCTTTTCTTTGTGGTAACGCAAATTTGTGTGATTGGGCATTATATGCAAATTGTAATGCTTTTAAATATATATATATTTTTTTTGTTCTAGAAGTACTTGATGTACTTCAGTGTTTATTGCAGGATTCATTTGCATTTTTAATAAGAGCAAGCTCAGCGTTGGGTTTGCCTCGGGGGACAGTGGAGAAAACAAGGGCTCGAGGGCCGGTGGGAGGGCTCCAGCGTGGGCTAGCCCGACCGAAGGTGGAGGCCCGAGCTCCAGGCCCGTGGCGAATTGCTATTTTTTTTTTGTGTCAGGCGCGTGCACTCCACTCGCGCGTGGGGGCGCGTCACCAACACAAAAATCAGAAGAAAAAAAAAAGTTTGTCAGTTACCATTGGGAAGCCACGTGGCTTCCCACGGTCCGTTAGATCGATCAACAGTTACTTAATTTGGACCGTTCGATCTCAACGCAAACGGTGGATATTAATATTCTTTATAAATAAAAAAATAAATGAAAAAATAGTTTTAAAATTAAGAAAAGTTACCGTTGTGACACGTAGCACAATCTGGAGTGTTGGAATTCAAATTTTTTTATATCCAACGATGAAAAAAAATGTAAAAAAAAATATTTTTACTTTTCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BolA

Protein Analysis

750

Amino Acids

86.14

Weight (kDa)

10.2

Isoelectric Point (pI)

41.39

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008532)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17560 AT5G17560 AT5G17560
fragaria_vesca	FvH4_4g10840 FvH4_4g10840
malus_domestica	MD08G1049500.v1.1 MD15G1045100.v1.1
prunus_persica	Prupe.1G398900_v2.0.a1
pyrus_communis	pycom08g04140 pycom15g04190 pycom15g04220
rosa_chinensis	RchiOBHm_Chr4g0406641
rosa_laevigata	RLG00000008763
rosa_multiflora	Rmu_co8238271.1_g000001 Rmu_sc0002774.1_g000004
rosa_roxburghii	Rroxscaffold_5G00351480
rosa_rugosa	Rorug04G0072600 Rorug04G0072600
rosa_samantha	Rh4AG139800 Rh4BG136800 Rh4CG147000 Rh4DG134000
rosa_wichuraiana	Rw4G011390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	209, 503, 2102
AarI	CACCTGC	1 cut(s)	676
Acc36I	ACCTGC	1 cut(s)	676
AccI	GTMKAC	2 cut(s)	715, 1131
AccII	CGCG	4 cut(s)	1924, 1939, 1941, 1951
AciI	CCGC	2 cut(s)	731, 1022
AclWI	GGATC	2 cut(s)	957, 970
AcoI	YGGCCR	2 cut(s)	732, 1057
AcsI	RAATTY	8 cut(s)	66, 148, 186, 365, 426, 1645, 2184, 2190
AcuI	CTGAAG	3 cut(s)	324, 1139, 1715
AcvI	CACGTG	1 cut(s)	2012
AfaI	GTAC	4 cut(s)	98, 670, 1719, 1728
AfeI	AGCGCT	3 cut(s)	478, 546, 581
AfiI	CCNNNNNNNGG	5 cut(s)	272, 399, 1066, 1835, 1864
AflIII	ACRYGT	2 cut(s)	759, 2159
AgsI	TTSAA	7 cut(s)	73, 103, 523, 945, 1242, 1493, 2189
AjnI	CCWGG	1 cut(s)	1882
AjuI	GAANNNNNNNTTGG	2 cut(s)	812, 844
AluBI	AGCT	9 cut(s)	465, 791, 941, 1015, 1153, 1397, 1621, 1776, 1880
AluI	AGCT	9 cut(s)	465, 791, 941, 1015, 1153, 1397, 1621, 1776, 1880
Alw21I	GWGCWC	5 cut(s)	693, 793, 1500, 1882, 1930
Alw26I	GTCTC	2 cut(s)	1087, 1490
Alw44I	GTGCAC	2 cut(s)	1496, 1926
AlwI	GGATC	2 cut(s)	957, 970
Ama87I	CYCGRG	5 cut(s)	936, 1234, 1794, 1821, 1875
Aor51HI	AGCGCT	3 cut(s)	478, 546, 581
AoxI	GGCC	7 cut(s)	293, 604, 732, 1057, 1826, 1872, 1885
ApaI	GGGCCC	1 cut(s)	297
ApaLI	GTGCAC	2 cut(s)	1496, 1926
ApoI	RAATTY	8 cut(s)	66, 148, 186, 365, 426, 1645, 2184, 2190
AseI	ATTAAT	1 cut(s)	2090
Asp700I	GAANNNNTTC	1 cut(s)	344
AspS9I	GGNCC	8 cut(s)	293, 294, 371, 1826, 1873, 1886, 2025, 2058
AsuC2I	CCSGG	1 cut(s)	1199
AsuHPI	GGTGA	3 cut(s)	55, 1375, 1946
AsuII	TTCGAA	2 cut(s)	575, 625
AsuNHI	GCTAGC	1 cut(s)	1852
AvaI	CYCGRG	5 cut(s)	936, 1234, 1794, 1821, 1875
AvaII	GGWCC	3 cut(s)	371, 2025, 2058
BaeGI	GKGCMC	3 cut(s)	297, 1500, 1930
BamHI	GGATCC	1 cut(s)	962
BanII	GRGCYC	5 cut(s)	297, 793, 1822, 1842, 1882
BbrPI	CACGTG	1 cut(s)	2012
BbsI	GAAGAC	2 cut(s)	496, 1588
Bbv12I	GWGCWC	5 cut(s)	693, 793, 1500, 1882, 1930
BccI	CCATC	6 cut(s)	34, 680, 805, 1049, 1120, 1562
BcgI	CGANNNNNNTGC	2 cut(s)	1917, 1951
BciT130I	CCWGG	1 cut(s)	1884
BclI	TGATCA	1 cut(s)	1177
BcnI	CCSGG	1 cut(s)	1199
BcoDI	GTCTC	2 cut(s)	1087, 1490
BfaI	CTAG	5 cut(s)	272, 486, 1347, 1712, 1853
BfmI	CTRYAG	1 cut(s)	456
BfoI	RGCGCY	6 cut(s)	393, 439, 480, 548, 583, 1587
BfuAI	ACCTGC	1 cut(s)	676
BisI	GCNGC	1 cut(s)	732
BlpI	GCTNAGC	1 cut(s)	1777
BlsI	GCNGC	1 cut(s)	733
BmcAI	AGTACT	1 cut(s)	1719
Bme1390I	CCNGG	2 cut(s)	1199, 1884
Bme18I	GGWCC	3 cut(s)	371, 2025, 2058
BmeT110I	CYCGRG	5 cut(s)	936, 1234, 1794, 1821, 1875
BmgT120I	GGNCC	8 cut(s)	293, 294, 371, 1826, 1873, 1886, 2025, 2058
BmiI	GGNNCC	4 cut(s)	295, 373, 964, 1841
BmrFI	CCNGG	2 cut(s)	1199, 1884
BmsI	GCATC	3 cut(s)	329, 858, 1405
BmtI	GCTAGC	1 cut(s)	1856
BpiI	GAAGAC	2 cut(s)	496, 1588
BpmI	CTGGAG	3 cut(s)	1826, 1866, 2194
Bpu1102I	GCTNAGC	1 cut(s)	1777
Bpu14I	TTCGAA	2 cut(s)	575, 625
BpuEI	CTTGAG	1 cut(s)	55
BpuMI	CCSGG	1 cut(s)	1199
Bsa29I	ATCGAT	1 cut(s)	2036
BsaAI	YACGTR	3 cut(s)	760, 2012, 2162
BsaJI	CCNNGG	5 cut(s)	815, 1197, 1793, 1889, 2021
BsaXI	ACNNNNNCTCC	2 cut(s)	1348, 1378
Bsc4I	CCNNNNNNNGG	5 cut(s)	272, 399, 1066, 1835, 1864
Bse118I	RCCGGY	3 cut(s)	1059, 1580, 1828
Bse3DI	GCAATG	2 cut(s)	493, 886
BseBI	CCWGG	1 cut(s)	1884
BseCI	ATCGAT	1 cut(s)	2036
BseDI	CCNNGG	5 cut(s)	815, 1197, 1793, 1889, 2021
BseGI	GGATG	6 cut(s)	45, 797, 1060, 1131, 1207, 1246
BseLI	CCNNNNNNNGG	5 cut(s)	272, 399, 1066, 1835, 1864
BseMI	GCAATG	2 cut(s)	493, 886
BseMII	CTCAG	1 cut(s)	1791
BseRI	GAGGAG	3 cut(s)	1025, 1075, 1163
BseSI	GKGCMC	3 cut(s)	297, 1500, 1930
BsgI	GTGCAG	2 cut(s)	63, 704
Bsh1236I	CGCG	4 cut(s)	1924, 1939, 1941, 1951
Bsh1285I	CGRYCG	1 cut(s)	1862
BshFI	GGCC	7 cut(s)	295, 606, 734, 1059, 1828, 1874, 1887
BshVI	ATCGAT	1 cut(s)	2036
BsiEI	CGRYCG	1 cut(s)	1862
BsiHKAI	GWGCWC	5 cut(s)	693, 793, 1500, 1882, 1930
BsiHKCI	CYCGRG	5 cut(s)	936, 1234, 1794, 1821, 1875
BsiSI	CCGG	4 cut(s)	1060, 1199, 1581, 1829
BslFI	GGGAC	4 cut(s)	580, 1113, 1217, 1813
BslI	CCNNNNNNNGG	5 cut(s)	272, 399, 1066, 1835, 1864
BsmAI	GTCTC	2 cut(s)	1087, 1490
BsmBI	CGTCTC	2 cut(s)	1087, 1490
BsmFI	GGGAC	4 cut(s)	580, 1113, 1217, 1813
BsmI	GAATGC	1 cut(s)	454
BsnI	GGCC	7 cut(s)	295, 606, 734, 1059, 1828, 1874, 1887
BsoBI	CYCGRG	5 cut(s)	936, 1234, 1794, 1821, 1875
Bsp119I	TTCGAA	2 cut(s)	575, 625
Bsp120I	GGGCCC	1 cut(s)	293
Bsp1286I	GDGCHC	8 cut(s)	297, 693, 793, 1500, 1822, 1842, 1882, 1930
Bsp143I	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
Bsp1720I	GCTNAGC	1 cut(s)	1777
BspACI	CCGC	2 cut(s)	731, 1022
BspANI	GGCC	7 cut(s)	295, 606, 734, 1059, 1828, 1874, 1887
BspCNI	CTCAG	1 cut(s)	1790
BspDI	ATCGAT	1 cut(s)	2036
BspFNI	CGCG	4 cut(s)	1924, 1939, 1941, 1951
BspLI	GGNNCC	4 cut(s)	295, 373, 964, 1841
BspMI	ACCTGC	1 cut(s)	676
BspOI	GCTAGC	1 cut(s)	1856
BspPI	GGATC	2 cut(s)	957, 970
BspT104I	TTCGAA	2 cut(s)	575, 625
BsrDI	GCAATG	2 cut(s)	493, 886
BsrFI	RCCGGY	3 cut(s)	1059, 1580, 1828
BssAI	RCCGGY	3 cut(s)	1059, 1580, 1828
BssECI	CCNNGG	5 cut(s)	815, 1197, 1793, 1889, 2021
BssMI	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
BssNAI	GTATAC	1 cut(s)	1132
BssT1I	CCWWGG	1 cut(s)	815
Bst1107I	GTATAC	1 cut(s)	1132
Bst2UI	CCWGG	1 cut(s)	1884
Bst6I	CTCTTC	2 cut(s)	1345, 1553
BstAPI	GCANNNNNTGC	1 cut(s)	1667
BstBAI	YACGTR	3 cut(s)	760, 2012, 2162
BstBI	TTCGAA	2 cut(s)	575, 625
BstC8I	GCNNGC	6 cut(s)	467, 1061, 1420, 1774, 1854, 1926
BstDEI	CTNAG	2 cut(s)	431, 1777
BstDSI	CCRYGG	2 cut(s)	1889, 2021
BstENI	CCTNNNNNAGG	1 cut(s)	270
BstF5I	GGATG	6 cut(s)	45, 797, 1060, 1131, 1207, 1246
BstFNI	CGCG	4 cut(s)	1924, 1939, 1941, 1951
BstH2I	RGCGCY	6 cut(s)	393, 439, 480, 548, 583, 1587
BstKTI	GATC	5 cut(s)	965, 1180, 2036, 2040, 2069
BstMAI	GTCTC	2 cut(s)	1087, 1490
BstMBI	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
BstMCI	CGRYCG	1 cut(s)	1862
BstMWI	GCNNNNNNNGC	3 cut(s)	776, 1667, 1778
BstNI	CCWGG	1 cut(s)	1884
BstNSI	RCATGY	1 cut(s)	1067
BstSCI	CCNGG	2 cut(s)	1197, 1882
BstSFI	CTRYAG	1 cut(s)	456
BstSLI	GKGCMC	3 cut(s)	297, 1500, 1930
BstUI	CGCG	4 cut(s)	1924, 1939, 1941, 1951
BstV2I	GAAGAC	2 cut(s)	496, 1588
BstX2I	RGATCY	1 cut(s)	962
BstXI	CCANNNNNNTGG	1 cut(s)	1890
BstYI	RGATCY	1 cut(s)	962
BstZ17I	GTATAC	1 cut(s)	1132
Bsu15I	ATCGAT	1 cut(s)	2036
BsuRI	GGCC	7 cut(s)	295, 606, 734, 1059, 1828, 1874, 1887
BsuTUI	ATCGAT	1 cut(s)	2036
BtgI	CCRYGG	2 cut(s)	1889, 2021
BtsCI	GGATG	6 cut(s)	45, 797, 1060, 1131, 1207, 1246
BtsIMutI	CAGTG	3 cut(s)	80, 1739, 1809
BveI	ACCTGC	1 cut(s)	676
Cac8I	GCNNGC	6 cut(s)	467, 1061, 1420, 1774, 1854, 1926
Cfr10I	RCCGGY	3 cut(s)	1059, 1580, 1828
Cfr13I	GGNCC	8 cut(s)	293, 294, 371, 1826, 1873, 1886, 2025, 2058
ClaI	ATCGAT	1 cut(s)	2036
CpoI	CGGWCCG	1 cut(s)	2025
CseI	GACGC	4 cut(s)	740, 937, 1219, 1940
Csp6I	GTAC	4 cut(s)	97, 669, 1718, 1727
CspI	CGGWCCG	1 cut(s)	2025
CviAII	CATG	7 cut(s)	286, 337, 745, 991, 1064, 1169, 1376
CviQI	GTAC	4 cut(s)	97, 669, 1718, 1727
DdeI	CTNAG	2 cut(s)	431, 1777
DpnI	GATC	5 cut(s)	964, 1179, 2035, 2039, 2068
DpnII	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
DraI	TTTAAA	3 cut(s)	64, 1687, 2133
EaeI	YGGCCR	2 cut(s)	732, 1057
Eam1104I	CTCTTC	2 cut(s)	1345, 1553
EarI	CTCTTC	2 cut(s)	1345, 1553
Ecl136II	GAGCTC	2 cut(s)	791, 1880
Eco130I	CCWWGG	1 cut(s)	815
Eco24I	GRGCYC	5 cut(s)	297, 793, 1822, 1842, 1882
Eco47I	GGWCC	3 cut(s)	371, 2025, 2058
Eco47III	AGCGCT	3 cut(s)	478, 546, 581
Eco53kI	GAGCTC	2 cut(s)	791, 1880
Eco57I	CTGAAG	3 cut(s)	324, 1139, 1715
Eco72I	CACGTG	1 cut(s)	2012
Eco88I	CYCGRG	5 cut(s)	936, 1234, 1794, 1821, 1875
EcoICRI	GAGCTC	2 cut(s)	791, 1880
EcoNI	CCTNNNNNAGG	1 cut(s)	270
EcoRI	GAATTC	1 cut(s)	2184
EcoRII	CCWGG	1 cut(s)	1882
EcoT14I	CCWWGG	1 cut(s)	815
EcoT38I	GRGCYC	5 cut(s)	297, 793, 1822, 1842, 1882
ErhI	CCWWGG	1 cut(s)	815
Esp3I	CGTCTC	2 cut(s)	1087, 1490
FaeI	CATG	7 cut(s)	289, 340, 748, 994, 1067, 1172, 1379
FalI	AAGNNNNNCTT	2 cut(s)	83, 115
FaqI	GGGAC	4 cut(s)	580, 1113, 1217, 1813
FatI	CATG	7 cut(s)	285, 336, 744, 990, 1063, 1168, 1375
FauNDI	CATATG	1 cut(s)	1142
FbaI	TGATCA	1 cut(s)	1177
FblI	GTMKAC	2 cut(s)	715, 1131
Fnu4HI	GCNGC	1 cut(s)	732
FokI	GGATG	6 cut(s)	52, 784, 1067, 1138, 1214, 1233
FriOI	GRGCYC	5 cut(s)	297, 793, 1822, 1842, 1882
Fsp4HI	GCNGC	1 cut(s)	732
FspBI	CTAG	5 cut(s)	272, 486, 1347, 1712, 1853
GluI	GCNGC	1 cut(s)	732
GsuI	CTGGAG	3 cut(s)	1826, 1866, 2194
HaeII	RGCGCY	6 cut(s)	393, 439, 480, 548, 583, 1587
HaeIII	GGCC	7 cut(s)	295, 606, 734, 1059, 1828, 1874, 1887
HapII	CCGG	4 cut(s)	1060, 1199, 1581, 1829
HgaI	GACGC	4 cut(s)	740, 937, 1219, 1940
Hin1II	CATG	7 cut(s)	289, 340, 748, 994, 1067, 1172, 1379
HincII	GTYRAC	2 cut(s)	223, 1331
HindII	GTYRAC	2 cut(s)	223, 1331
HindIII	AAGCTT	2 cut(s)	463, 1395
HinfI	GANTC	6 cut(s)	927, 971, 986, 1025, 1359, 1748
HpaII	CCGG	4 cut(s)	1060, 1199, 1581, 1829
HphI	GGTGA	3 cut(s)	55, 1375, 1946
Hpy166II	GTNNAC	6 cut(s)	223, 716, 1132, 1331, 1498, 1928
Hpy188I	TCNGA	9 cut(s)	304, 588, 854, 902, 953, 1158, 1182, 1462, 1971
Hpy188III	TCNNGA	5 cut(s)	236, 1234, 1600, 1712, 2173
Hpy8I	GTNNAC	6 cut(s)	223, 716, 1132, 1331, 1498, 1928
Hpy99I	CGWCG	2 cut(s)	953, 1415
HpyAV	CCTTC	2 cut(s)	1262, 1858
HpyCH4IV	ACGT	5 cut(s)	108, 179, 759, 2011, 2161
HpyF10VI	GCNNNNNNNGC	3 cut(s)	776, 1667, 1778
HpyF3I	CTNAG	2 cut(s)	431, 1777
HpySE526I	ACGT	5 cut(s)	108, 179, 759, 2011, 2161
Hsp92II	CATG	7 cut(s)	289, 340, 748, 994, 1067, 1172, 1379
KroI	GCCGGC	1 cut(s)	1059
KroNI	GCCGGC	1 cut(s)	1061
Ksp22I	TGATCA	1 cut(s)	1177
Kzo9I	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
LmnI	GCTCC	6 cut(s)	891, 1012, 1150, 1462, 1845, 1885
LweI	GCATC	3 cut(s)	329, 858, 1405
MaeI	CTAG	5 cut(s)	272, 486, 1347, 1712, 1853
MaeII	ACGT	5 cut(s)	108, 179, 759, 2011, 2161
MaeIII	GTNAC	7 cut(s)	1288, 1637, 1952, 1993, 2045, 2146, 2155
MalI	GATC	5 cut(s)	964, 1179, 2035, 2039, 2068
MboI	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
MfeI	CAATTG	2 cut(s)	873, 1275
MflI	RGATCY	1 cut(s)	962
MhlI	GDGCHC	8 cut(s)	297, 693, 793, 1500, 1822, 1842, 1882, 1930
MlyI	GAGTC	1 cut(s)	965
MmeI	TCCRAC	5 cut(s)	931, 1261, 1440, 2161, 2228
MroNI	GCCGGC	1 cut(s)	1059
MroXI	GAANNNNTTC	1 cut(s)	344
MslI	CAYNNNNRTG	3 cut(s)	85, 680, 707
MspI	CCGG	4 cut(s)	1060, 1199, 1581, 1829
MspR9I	CCNGG	2 cut(s)	1199, 1884
MunI	CAATTG	2 cut(s)	873, 1275
Mva1269I	GAATGC	1 cut(s)	454
MvaI	CCWGG	1 cut(s)	1884
MvnI	CGCG	4 cut(s)	1924, 1939, 1941, 1951
MwoI	GCNNNNNNNGC	3 cut(s)	776, 1667, 1778
NaeI	GCCGGC	1 cut(s)	1061
NciI	CCSGG	1 cut(s)	1199
NdeI	CATATG	1 cut(s)	1142
NdeII	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
NgoMIV	GCCGGC	1 cut(s)	1059
NheI	GCTAGC	1 cut(s)	1852
NlaIII	CATG	7 cut(s)	289, 340, 748, 994, 1067, 1172, 1379
NlaIV	GGNNCC	4 cut(s)	295, 373, 964, 1841
NmuCI	GTSAC	2 cut(s)	1952, 2155
NspI	RCATGY	1 cut(s)	1067
NspV	TTCGAA	2 cut(s)	575, 625
PaeR7I	CTCGAG	2 cut(s)	936, 1821
PaqCI	CACCTGC	1 cut(s)	676
PctI	GAATGC	1 cut(s)	454
PdiI	GCCGGC	1 cut(s)	1061
PdmI	GAANNNNTTC	1 cut(s)	344
PfeI	GAWTC	5 cut(s)	927, 986, 1025, 1359, 1748
PkrI	GCNGC	1 cut(s)	733
PleI	GAGTC	1 cut(s)	965
PmaCI	CACGTG	1 cut(s)	2012
PmlI	CACGTG	1 cut(s)	2012
PpsI	GAGTC	1 cut(s)	965
Ppu21I	YACGTR	3 cut(s)	760, 2012, 2162
PshBI	ATTAAT	1 cut(s)	2090
PsiI	TTATAA	3 cut(s)	209, 503, 2102
Psp124BI	GAGCTC	2 cut(s)	793, 1882
Psp6I	CCWGG	1 cut(s)	1882
PspCI	CACGTG	1 cut(s)	2012
PspGI	CCWGG	1 cut(s)	1882
PspN4I	GGNNCC	4 cut(s)	295, 373, 964, 1841
PspOMI	GGGCCC	1 cut(s)	293
PspPI	GGNCC	8 cut(s)	293, 294, 371, 1826, 1873, 1886, 2025, 2058
PspXI	VCTCGAGB	2 cut(s)	936, 1821
PsuI	RGATCY	1 cut(s)	962
RsaI	GTAC	4 cut(s)	98, 670, 1719, 1728
RsaNI	GTAC	4 cut(s)	97, 669, 1718, 1727
RseI	CAYNNNNRTG	3 cut(s)	85, 680, 707
Rsr2I	CGGWCCG	1 cut(s)	2025
RsrII	CGGWCCG	1 cut(s)	2025
SacI	GAGCTC	2 cut(s)	793, 1882
SatI	GCNGC	1 cut(s)	732
Sau3AI	GATC	5 cut(s)	962, 1177, 2033, 2037, 2066
Sau96I	GGNCC	8 cut(s)	293, 294, 371, 1826, 1873, 1886, 2025, 2058
ScaI	AGTACT	1 cut(s)	1719
SchI	GAGTC	1 cut(s)	965
ScrFI	CCNGG	2 cut(s)	1199, 1884
SduI	GDGCHC	8 cut(s)	297, 693, 793, 1500, 1822, 1842, 1882, 1930
SfaNI	GCATC	3 cut(s)	329, 858, 1405
SfcI	CTRYAG	1 cut(s)	456
Sfr274I	CTCGAG	2 cut(s)	936, 1821
SfuI	TTCGAA	2 cut(s)	575, 625
SgrAI	CRCCGGYG	1 cut(s)	1580
SinI	GGWCC	3 cut(s)	371, 2025, 2058
SlaI	CTCGAG	2 cut(s)	936, 1821
SmiMI	CAYNNNNRTG	3 cut(s)	85, 680, 707
SmlI	CTYRAG	3 cut(s)	34, 936, 1821
SmoI	CTYRAG	3 cut(s)	34, 936, 1821
SsiI	CCGC	2 cut(s)	731, 1022
SspI	AATATT	5 cut(s)	56, 612, 1322, 2094, 2233
SspMI	CTAG	5 cut(s)	272, 486, 1347, 1712, 1853
SstI	GAGCTC	2 cut(s)	793, 1882
StyD4I	CCNGG	2 cut(s)	1197, 1882
StyI	CCWWGG	1 cut(s)	815
TaiI	ACGT	5 cut(s)	111, 182, 762, 2014, 2164
TaqII	GACCGA	1 cut(s)	1876
TatI	WGTACW	2 cut(s)	1717, 1726
TauI	GCSGC	1 cut(s)	734
TfiI	GAWTC	5 cut(s)	927, 986, 1025, 1359, 1748
TscAI	CASTG	3 cut(s)	87, 1739, 1809
TseFI	GTSAC	2 cut(s)	1952, 2155
Tsp45I	GTSAC	2 cut(s)	1952, 2155
TspGWI	ACGGA	2 cut(s)	1018, 2017
TspRI	CASTG	3 cut(s)	87, 1739, 1809
VneI	GTGCAC	2 cut(s)	1496, 1926
VpaK11BI	GGWCC	3 cut(s)	371, 2025, 2058
VspI	ATTAAT	1 cut(s)	2090
XagI	CCTNNNNNAGG	1 cut(s)	270
XapI	RAATTY	8 cut(s)	66, 148, 186, 365, 426, 1645, 2184, 2190
XbaI	TCTAGA	1 cut(s)	1711
XceI	RCATGY	1 cut(s)	1067
XcmI	CCANNNNNNNNNTGG	2 cut(s)	742, 872
XhoI	CTCGAG	2 cut(s)	936, 1821
XmiI	GTMKAC	2 cut(s)	715, 1131
XmnI	GAANNNNTTC	1 cut(s)	344
XspI	CTAG	5 cut(s)	272, 486, 1347, 1712, 1853
ZrmI	AGTACT	1 cut(s)	1719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.