MD15G1045100.v1.1

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008532
Physical Location & Seq
Forward (+)
3088977 .. 3090817
1841 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1045100.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGCGGCCAACTCAATCATGGTGCGTCCGTATGTGTTATCAGCCTCCTGCGCCTTTACTCGAGCTCTGCCATCCTTACTTTCTCAACCAAGGAAAACACTGTTGGTATTTAAGAGCCGGGGCATCAGATTGTTGCACCATAGAGCAATTGGCAATGAAAACAGGAGCCTTATGAGATTGGAATACGGTGGTGTGAGGAGATTCAGTCCTCGAGCTTCAAGCGTCGGAAATGCTGGCTCCATTGACTCCCCACTAATAGAATCCATGAAGAAAAAGATCAAGGAGGAGCTAAATGCGGAATCCGTTAGTGTAGAAGATATGAACGGGGATGGCCGGCATGTGGTCATTGATGTCGTCTCCTCGTCATTTGAGGGACAGTCGGCTGTAAATAGGCAGAGGATGGTGTACAAAGCCATATGGGAGGAGCTTCAGAACACAGTTCATGCAGTTGATCAGATGACTACCAAAACCCCGGATGAAGTTTCTGGTAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

166

Amino Acids

18.3

Weight (kDa)

9.8

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 99 - 160 7.1e-14 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 296
AcoI YGGCCR 2 cut(s) 6, 331
AcuI CTGAAG 1 cut(s) 413
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 340
AgsI TTSAA 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AluBI AGCT 4 cut(s) 65, 215, 289, 427
AluI AGCT 4 cut(s) 65, 215, 289, 427
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 361
Ama87I CYCGRG 2 cut(s) 60, 210
AoxI GGCC 2 cut(s) 6, 331
AspLEI GCGC 1 cut(s) 53
AsuC2I CCSGG 2 cut(s) 119, 473
AvaI CYCGRG 2 cut(s) 60, 210
BanII GRGCYC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 67
BccI CCATC 3 cut(s) 79, 323, 394
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 2 cut(s) 119, 473
BcoDI GTCTC 1 cut(s) 361
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 119, 473
BmeT110I CYCGRG 2 cut(s) 60, 210
BmiI GGNNCC 2 cut(s) 167, 238
BmrFI CCNGG 2 cut(s) 119, 473
BmsI GCATC 1 cut(s) 132
BpuMI CCSGG 2 cut(s) 119, 473
BsaJI CCNNGG 3 cut(s) 89, 118, 471
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse118I RCCGGY 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 160
BseDI CCNNGG 3 cut(s) 89, 118, 471
BseGI GGATG 4 cut(s) 71, 334, 405, 481
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMI GCAATG 1 cut(s) 160
BseRI GAGGAG 4 cut(s) 211, 299, 349, 437
BshFI GGCC 2 cut(s) 8, 333
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 2 cut(s) 60, 210
BsiSI CCGG 3 cut(s) 118, 334, 473
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 1 cut(s) 361
BsmBI CGTCTC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 387
BsnI GGCC 2 cut(s) 8, 333
BsoBI CYCGRG 2 cut(s) 60, 210
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 2 cut(s) 276, 451
BspACI CCGC 2 cut(s) 5, 296
BspANI GGCC 2 cut(s) 8, 333
BspLI GGNNCC 2 cut(s) 167, 238
BsrDI GCAATG 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 333
BsrGI TGTACA 1 cut(s) 405
BssAI RCCGGY 1 cut(s) 333
BssECI CCNNGG 3 cut(s) 89, 118, 471
BssMI GATC 2 cut(s) 276, 451
BssT1I CCWWGG 1 cut(s) 89
Bst4CI ACNGT 4 cut(s) 102, 188, 378, 439
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 2 cut(s) 235, 335
BstF5I GGATG 4 cut(s) 71, 334, 405, 481
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 2 cut(s) 279, 454
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 2 cut(s) 276, 451
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 341
BstSCI CCNGG 2 cut(s) 117, 471
BsuRI GGCC 2 cut(s) 8, 333
BtsCI GGATG 4 cut(s) 71, 334, 405, 481
BtsIMutI CAGTG 1 cut(s) 98
Cac8I GCNNGC 2 cut(s) 235, 335
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 333
CseI GACGC 2 cut(s) 14, 211
Csp6I GTAC 1 cut(s) 406
CviAII CATG 4 cut(s) 19, 265, 338, 443
CviQI GTAC 1 cut(s) 406
DpnI GATC 2 cut(s) 278, 453
DpnII GATC 2 cut(s) 276, 451
EaeI YGGCCR 2 cut(s) 6, 331
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 413
Eco88I CYCGRG 2 cut(s) 60, 210
EcoICRI GAGCTC 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 89
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 4 cut(s) 22, 268, 341, 446
FaqI GGGAC 1 cut(s) 387
FatI CATG 4 cut(s) 18, 264, 337, 442
FauNDI CATATG 1 cut(s) 416
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 4 cut(s) 58, 341, 412, 488
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 1 cut(s) 52
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 2 cut(s) 8, 333
HapII CCGG 3 cut(s) 118, 334, 473
HgaI GACGC 2 cut(s) 14, 211
HhaI GCGC 1 cut(s) 53
Hin1II CATG 4 cut(s) 22, 268, 341, 446
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 4 cut(s) 201, 245, 260, 299
HpaII CCGG 3 cut(s) 118, 334, 473
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 4 cut(s) 128, 227, 432, 456
Hpy8I GTNNAC 1 cut(s) 406
Hpy99I CGWCG 1 cut(s) 227
HpyCH4III ACNGT 4 cut(s) 102, 188, 378, 439
HpyCH4V TGCA 2 cut(s) 136, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
Hsp92II CATG 4 cut(s) 22, 268, 341, 446
HspAI GCGC 1 cut(s) 51
KroI GCCGGC 1 cut(s) 333
KroNI GCCGGC 1 cut(s) 335
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 2 cut(s) 276, 451
LmnI GCTCC 4 cut(s) 165, 242, 286, 424
LpnPI CCDG 7 cut(s) 61, 131, 148, 219, 347, 471, 486
LweI GCATC 1 cut(s) 132
MalI GATC 2 cut(s) 278, 453
MboI GATC 2 cut(s) 276, 451
MboII GAAGA 2 cut(s) 280, 326
MfeI CAATTG 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 1 cut(s) 147
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 8 cut(s) 55, 189, 219, 277, 364, 370, 390, 415
MroNI GCCGGC 1 cut(s) 333
MseI TTAA 1 cut(s) 111
MspI CCGG 3 cut(s) 118, 334, 473
MspR9I CCNGG 2 cut(s) 119, 473
MunI CAATTG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 50
NaeI GCCGGC 1 cut(s) 335
NciI CCSGG 2 cut(s) 119, 473
NdeI CATATG 1 cut(s) 416
NdeII GATC 2 cut(s) 276, 451
NgoMIV GCCGGC 1 cut(s) 333
NlaIII CATG 4 cut(s) 22, 268, 341, 446
NlaIV GGNNCC 2 cut(s) 167, 238
NspI RCATGY 1 cut(s) 341
PaeR7I CTCGAG 2 cut(s) 60, 210
PdiI GCCGGC 1 cut(s) 335
PfeI GAWTC 3 cut(s) 201, 260, 299
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 67
PspN4I GGNNCC 2 cut(s) 167, 238
PspXI VCTCGAGB 2 cut(s) 60, 210
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 276, 451
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 119, 473
SduI GDGCHC 1 cut(s) 67
SetI ASST 4 cut(s) 67, 217, 291, 429
SfaNI GCATC 1 cut(s) 132
Sfr274I CTCGAG 2 cut(s) 60, 210
SlaI CTCGAG 2 cut(s) 60, 210
SmlI CTYRAG 2 cut(s) 60, 210
SmoI CTYRAG 2 cut(s) 60, 210
Sse9I AATT 1 cut(s) 147
SsiI CCGC 2 cut(s) 5, 296
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 117, 471
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 4 cut(s) 102, 188, 378, 439
TaqI TCGA 2 cut(s) 61, 211
TasI AATT 1 cut(s) 147
TatI WGTACW 1 cut(s) 405
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 201, 260, 299
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 105
TspDTI ATGAA 5 cut(s) 171, 281, 335, 431, 492
TspGWI ACGGA 2 cut(s) 18, 292
TspRI CASTG 1 cut(s) 105
XceI RCATGY 1 cut(s) 341
XcmI CCANNNNNNNNNTGG 2 cut(s) 16, 146
XhoI CTCGAG 2 cut(s) 60, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.