RLG00000008763

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008532
Physical Location & Seq
Reverse (-)
36666922 .. 36667888
967 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008763

Sequence Viewer

Length: 612 bp
ATGGTGGCTGGGATGGTGATGGTGCGTCCGTATGTGTCAGCCTCCACACTAAGTCGAGCTCTGCCATCAGCAGCCTCTGCACTAAGTCGAGCTTTACCAACCTTATTTGCTGAACCAAGGAATAATTGGTTAGTGTGTGAGACTCAGAGCATGAAATTGTTAAATGATAGAGCAACCGCCAACAAGCACAGAAACTCAAGGAGACTGGTGTTTGGTGGTGGGAGGAGGTTCACTACTCGAGCTTCCCACGTCAATGATGCTGGCTACATTGGCTCGCCTCTCATGCAGTCCATGGAGAACAAGATCAAGGAACAGCTGAATGCAGAATCTGTTATTGTTAAAGATGCTAACGGGGATGGTCGGCATGTCAGCATTGATGTTGTCTCTTCATCCTTTGAGGGCCAATCTGCTGTGAATAGGCAGAGGATGGTGTACAAAGCCATATGGGAGGAGCTTCAAAACACAGTGCATGCAGTAGATCAGATGACTACTAAAACCCCAGATGAAGCAGCAACACAAAAGAGGTTACTTTATCATGTTACTTCAGGGTCTATTTCGTTGCAGCCACTGGTAGAAGCCAAGACTGCTCCAAGGCCACTGCTCGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BolA

Protein Analysis

204

Amino Acids

22.31

Weight (kDa)

9.8

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 116 - 169 5.2e-14 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 177
AcuI CTGAAG 1 cut(s) 528
AfaI GTAC 1 cut(s) 434
AgsI TTSAA 1 cut(s) 458
AjiI CACGTC 1 cut(s) 250
AluBI AGCT 5 cut(s) 59, 92, 242, 316, 454
AluI AGCT 5 cut(s) 59, 92, 242, 316, 454
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 3 cut(s) 134, 196, 388
AlwNI CAGNNNCTG 3 cut(s) 77, 329, 568
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 2 cut(s) 400, 593
ApeKI GCWGC 3 cut(s) 71, 509, 562
AspS9I GGNCC 1 cut(s) 400
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 237
BanII GRGCYC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 3 cut(s) 83, 521, 574
BccI CCATC 5 cut(s) 7, 13, 73, 350, 421
BcoDI GTCTC 3 cut(s) 134, 196, 388
BisI GCNGC 3 cut(s) 72, 510, 563
BlsI GCNGC 3 cut(s) 73, 511, 564
BmeT110I CYCGRG 1 cut(s) 237
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 400
BmsI GCATC 3 cut(s) 247, 334, 595
BpuEI CTTGAG 1 cut(s) 181
BsaJI CCNNGG 3 cut(s) 116, 291, 590
Bse1I ACTGG 2 cut(s) 210, 573
BseDI CCNNGG 3 cut(s) 116, 291, 590
BseGI GGATG 4 cut(s) 18, 361, 389, 432
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 210, 573
BseRI GAGGAG 2 cut(s) 238, 464
BseXI GCAGC 3 cut(s) 83, 521, 574
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 2 cut(s) 402, 595
BsiHKAI GWGCWC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 237
BsmAI GTCTC 3 cut(s) 134, 196, 388
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 2 cut(s) 402, 595
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 2 cut(s) 303, 478
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 1 cut(s) 177
BspANI GGCC 2 cut(s) 402, 595
BspCNI CTCAG 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 2 cut(s) 210, 573
BssECI CCNNGG 3 cut(s) 116, 291, 590
BssMI GATC 2 cut(s) 303, 478
BssT1I CCWWGG 3 cut(s) 116, 291, 590
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 3 cut(s) 262, 275, 471
BstDEI CTNAG 3 cut(s) 50, 83, 144
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 4 cut(s) 18, 361, 389, 432
BstKTI GATC 2 cut(s) 306, 481
BstMAI GTCTC 3 cut(s) 134, 196, 388
BstMBI GATC 2 cut(s) 303, 478
BstMWI GCNNNNNNNGC 4 cut(s) 77, 270, 283, 584
BstNSI RCATGY 2 cut(s) 368, 473
BstV1I GCAGC 3 cut(s) 83, 521, 574
BsuRI GGCC 2 cut(s) 402, 595
BtgI CCRYGG 1 cut(s) 291
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 4 cut(s) 18, 361, 389, 432
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 3 cut(s) 471, 566, 596
Cac8I GCNNGC 3 cut(s) 262, 275, 471
CaiI CAGNNNCTG 3 cut(s) 77, 329, 568
Cfr13I GGNCC 1 cut(s) 400
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 433
CviAII CATG 6 cut(s) 151, 283, 292, 365, 470, 536
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 3 cut(s) 50, 83, 144
DpnI GATC 2 cut(s) 305, 480
DpnII GATC 2 cut(s) 303, 478
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Ecl136II GAGCTC 1 cut(s) 59
Eco130I CCWWGG 3 cut(s) 116, 291, 590
Eco24I GRGCYC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 59
EcoT14I CCWWGG 3 cut(s) 116, 291, 590
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 3 cut(s) 116, 291, 590
FaeI CATG 6 cut(s) 154, 286, 295, 368, 473, 539
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FatI CATG 6 cut(s) 150, 282, 291, 364, 469, 535
FauNDI CATATG 1 cut(s) 443
Fnu4HI GCNGC 3 cut(s) 72, 510, 563
FokI GGATG 4 cut(s) 25, 368, 376, 439
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 3 cut(s) 72, 510, 563
GluI GCNGC 3 cut(s) 72, 510, 563
GsaI CCCAGC 1 cut(s) 12
HaeIII GGCC 2 cut(s) 402, 595
HgaI GACGC 1 cut(s) 14
Hin1II CATG 6 cut(s) 154, 286, 295, 368, 473, 539
HinfI GANTC 2 cut(s) 142, 326
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 231, 433
Hpy188I TCNGA 2 cut(s) 147, 483
Hpy8I GTNNAC 2 cut(s) 231, 433
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 7 cut(s) 80, 286, 323, 469, 473, 562, 608
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 270, 283, 584
HpyF3I CTNAG 3 cut(s) 50, 83, 144
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 6 cut(s) 154, 286, 295, 368, 473, 539
Kzo9I GATC 2 cut(s) 303, 478
LmnI GCTCC 2 cut(s) 451, 592
LpnPI CCDG 5 cut(s) 191, 246, 513, 531, 554
Lsp1109I GCAGC 3 cut(s) 83, 521, 574
LweI GCATC 3 cut(s) 247, 334, 595
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 2 cut(s) 525, 538
MalI GATC 2 cut(s) 305, 480
MboI GATC 2 cut(s) 303, 478
MboII GAAGA 1 cut(s) 378
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 2 cut(s) 124, 155
MlyI GAGTC 1 cut(s) 136
MnlI CCTC 9 cut(s) 52, 85, 216, 219, 288, 391, 417, 442, 516
Mph1103I ATGCAT 1 cut(s) 610
MseI TTAA 2 cut(s) 161, 339
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 316
Mva1269I GAATGC 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 77, 270, 283, 584
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 443
NdeII GATC 2 cut(s) 303, 478
NlaIII CATG 6 cut(s) 154, 286, 295, 368, 473, 539
NsiI ATGCAT 1 cut(s) 610
NspI RCATGY 2 cut(s) 368, 473
PaeI GCATGC 1 cut(s) 473
PaeR7I CTCGAG 1 cut(s) 237
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 3 cut(s) 73, 511, 564
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 8
PspPI GGNCC 1 cut(s) 400
PspXI VCTCGAGB 1 cut(s) 237
PstNI CAGNNNCTG 3 cut(s) 77, 329, 568
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 252
SacI GAGCTC 1 cut(s) 61
SaqAI TTAA 2 cut(s) 161, 339
SatI GCNGC 3 cut(s) 72, 510, 563
Sau3AI GATC 2 cut(s) 303, 478
Sau96I GGNCC 1 cut(s) 400
SchI GAGTC 1 cut(s) 136
SduI GDGCHC 1 cut(s) 61
SetI ASST 9 cut(s) 61, 94, 104, 230, 244, 252, 318, 456, 527
SfaNI GCATC 3 cut(s) 247, 334, 595
Sfr274I CTCGAG 1 cut(s) 237
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 196, 237
SmoI CTYRAG 2 cut(s) 196, 237
SphI GCATGC 1 cut(s) 473
Sse9I AATT 2 cut(s) 124, 155
SsiI CCGC 1 cut(s) 177
SstI GAGCTC 1 cut(s) 61
StyI CCWWGG 3 cut(s) 116, 291, 590
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 1 cut(s) 252
TaqI TCGA 4 cut(s) 55, 88, 238, 603
TasI AATT 2 cut(s) 124, 155
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 161, 339
Tru9I TTAA 2 cut(s) 161, 339
TscAI CASTG 3 cut(s) 471, 573, 603
TseI GCWGC 3 cut(s) 71, 509, 562
TspDTI ATGAA 3 cut(s) 167, 378, 519
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 3 cut(s) 471, 573, 603
XceI RCATGY 2 cut(s) 368, 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 123
XhoI CTCGAG 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.