FvH4_4g11480

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012605
Physical Location & Seq
Reverse (-)
15239382 .. 15244554
5173 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11480.t1

Sequence Viewer

Length: 1668 bp
ATGCTCTTAATGTTATCCACCGCTAAAACTAAACATGGAGCTCTCACTCTTTCTTCAATTCTCTTCAAACACTTTTCTTCAAATTCCACAACCCCAAATCAAATTTCCACAATCCCAAAACAACCCCTGGCACCAACTGCTTCTGTTCTATGTTCTCTGCTCTCTAAAGAAACCACCGCTAGTGTTGATAGTCTGTTGGGTAGTTTCAAACAACAGTTGAGTTCAGAACTGGTGCTCCAAATCTTGATGAATTATAAGCAGCTGGGAAGGAGAAAGACTCTCGACTTCTTTTCCTGGGCTGGGCTTCAAATGCATTTTCAGTTTGATGACTGTGTGGTTGAGTTCATGGCTGATTTCTTAGGTAGGAGGAAGCTCTTTGATGACATGAAGCATCTTTTATTGACTGTGTCATTGCACAAGGGTGGTGTTTCGTGTCGAGCAGTTTCGACTTGTATTAGGTTTCTGGGTAGGGAGGGGAGGATTAGAGAGGCACTTTGTGTGTTTCAGGAAATGGAATCTAGATTTGGGTGTAAGCCTGATAATCTTGTGTACAATAATGTGCTTTACGTGCTTTGTAAGAAGGATTCATCCGGGCATTTGATTGATTTCGCGCTTGCTGTTTTCCGGAGAATTGAATCCCCTGATGTGTATTCGTATAGTAATATGCTTGTTGGTTTGTGTAAATTCGGTAGGTTTGAGACTGCTGTTGAGGTTTTTGGTGAAATGTGTAGTACTGGTTTGGTTCCTACTAGGTCTGCTGTGAATGTTCTTATTGGAGAGCTGTGTTTGTTGAGTGCGAAAGAAGCAGCTGTTGCGAAAGTAAGAGTGAAAAATGTTCGCAGGCCATTTAAGATTTTGGTTCCAAATGTGCATGAAAAGAGTGGTGCTATCCAGCCTGCAGTTGGAGTGTTTTGGGAAGTTTATAAGTTAGGCTTGTTACCTAGTACTTTTGTTATAATCCGACTTCTATTGGAGCTTTCTAAACTAGGGAAAATGGAAGAGGCTGTTGGGGTCTTGAGGGCTGTTGAGGGTAGGAAGCTGAGTTGTGTTGAAGAGGGGTACTGTATTGTGATGAAGGGTTTGTGTGAACACTGCTGTGTTGAGGAAGCTAGTCGTTTGTTTGGGAGGATGCTCTCTCAGGGCATGAAGCCGAGGTTGACTATTTATAATTCTGTTATTTGCATGCTATGCAAACTAGGGAATTTGGATGGTGCTGAAAGTGTCTTCAAGATGATGAACAAGAAGAGATGTCTTCCAGATAGTTGCACTTATTCTGCATTGGTCCATGCTTATTGTGAAGCTGGAAAATGGAAAGCTGCTTATGACCTCATGATAGAAATGCTTGGGTTGGGTTGCTCTCCACCTTTTCACACTTATAGTTCAGTGGATAAACTTTTGAGAGAAAATGAACAAACGGATTTGTGTTTTAAATTGGAAAGGAAGTTGGAAACCCAGATCTTGGAGAAGCTATGCAAAGATGGTCAATTAGATGATGCGTATGAGAAGCTAAAGTCAATGGTTGAAAAGGGGTTTTACCCACCAGTGTATGCGAGAGATTCTTTTAAGAATGCATTTCGTAAGTATGGAAAATTGAATATAGCTCGGGATTTGTTACAGACGATAGATGACATGGACAACAACGAGGAGAAACAACTTTGCACTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

556

Amino Acids

62.44

Weight (kDa)

8.76

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 150 - 193 2.9e-06 PPR repeat family
PPR_1 PF12854 213 - 242 1.2e-06 PPR repeat
PPR_2 PF13041 214 - 262 5.8e-09 PPR repeat family
PPR_long PF17177 366 - 451 8.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 373 - 431 7.4e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 387 - 432 8.6e-13 PPR repeat family
PPR PF01535 388 - 417 2.1e-06 PPR repeat
PPR_1 PF12854 416 - 447 1.6e-08 PPR repeat
PPR_2 PF13041 422 - 461 4.3e-08 PPR repeat family
PPR PF01535 423 - 452 6.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 255, 924, 956, 1167
AccB1I GGYRCC 1 cut(s) 130
AccB7I CCANNNNNTGG 1 cut(s) 1459
AccII CGCG 1 cut(s) 611
AccIII TCCGGA 1 cut(s) 624
AciI CCGC 2 cut(s) 21, 177
AcsI RAATTY 4 cut(s) 82, 102, 683, 1201
AdeI CACNNNGTG 1 cut(s) 497
AfaI GTAC 4 cut(s) 551, 733, 946, 1061
AfiI CCNNNNNNNGG 3 cut(s) 300, 902, 1459
AjnI CCWGG 2 cut(s) 126, 293
AjuI GAANNNNNNNTTGG 6 cut(s) 88, 120, 507, 539, 990, 1022
AleI CACNNNNGTG 2 cut(s) 420, 1095
AloI GAACNNNNNNTCC 2 cut(s) 219, 251
Alw21I GWGCWC 2 cut(s) 43, 237
Alw26I GTCTC 1 cut(s) 692
Ama87I CYCGRG 1 cut(s) 1602
Aor13HI TCCGGA 1 cut(s) 624
AoxI GGCC 1 cut(s) 842
ApeKI GCWGC 3 cut(s) 259, 806, 1316
ApoI RAATTY 4 cut(s) 82, 102, 683, 1201
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 1 cut(s) 1282
AsuC2I CCSGG 1 cut(s) 592
AsuHPI GGTGA 1 cut(s) 731
AvaI CYCGRG 1 cut(s) 1602
AvaII GGWCC 1 cut(s) 1282
BanI GGYRCC 1 cut(s) 130
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 2 cut(s) 1216, 1244
Bbv12I GWGCWC 2 cut(s) 43, 237
BbvI GCAGC 3 cut(s) 271, 818, 1303
BccI CCATC 2 cut(s) 1202, 1472
BciT130I CCWGG 2 cut(s) 128, 295
BcnI CCSGG 1 cut(s) 592
BcoDI GTCTC 1 cut(s) 692
BfaI CTAG 7 cut(s) 180, 519, 750, 942, 986, 1110, 1196
BfmI CTRYAG 1 cut(s) 897
BglII AGATCT 1 cut(s) 1455
BisI GCNGC 3 cut(s) 260, 807, 1317
BlsI GCNGC 3 cut(s) 261, 808, 1318
BmcAI AGTACT 2 cut(s) 733, 946
Bme1390I CCNGG 3 cut(s) 128, 295, 592
Bme18I GGWCC 1 cut(s) 1282
BmeT110I CYCGRG 1 cut(s) 1602
BmgT120I GGNCC 1 cut(s) 1282
BmiI GGNNCC 3 cut(s) 132, 744, 861
BmrFI CCNGG 3 cut(s) 128, 295, 592
BmsI GCATC 3 cut(s) 400, 1119, 1483
BpiI GAAGAC 2 cut(s) 1216, 1244
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpuEI CTTGAG 1 cut(s) 1036
BpuMI CCSGG 1 cut(s) 592
BsaAI YACGTR 1 cut(s) 568
BsaJI CCNNGG 3 cut(s) 126, 294, 1151
BsaWI WCCGGW 1 cut(s) 624
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 902, 1459
Bse1I ACTGG 3 cut(s) 234, 739, 1541
Bse3DI GCAATG 1 cut(s) 410
BseAI TCCGGA 1 cut(s) 624
BseBI CCWGG 2 cut(s) 128, 295
BseDI CCNNGG 3 cut(s) 126, 294, 1151
BseGI GGATG 3 cut(s) 587, 1134, 1213
BseLI CCNNNNNNNGG 3 cut(s) 300, 902, 1459
BseMI GCAATG 1 cut(s) 410
BseMII CTCAG 2 cut(s) 1031, 1151
BseNI ACTGG 3 cut(s) 234, 739, 1541
BseRI GAGGAG 1 cut(s) 1658
BseXI GCAGC 3 cut(s) 271, 818, 1303
BseYI CCCAGC 2 cut(s) 262, 299
Bsh1236I CGCG 1 cut(s) 611
BshFI GGCC 1 cut(s) 844
BshNI GGYRCC 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 43, 237
BsiHKCI CYCGRG 1 cut(s) 1602
BsiSI CCGG 2 cut(s) 591, 625
BslI CCNNNNNNNGG 3 cut(s) 300, 902, 1459
BsmAI GTCTC 1 cut(s) 692
BsmI GAATGC 1 cut(s) 1573
BsnI GGCC 1 cut(s) 844
BsoBI CYCGRG 1 cut(s) 1602
Bsp1286I GDGCHC 2 cut(s) 43, 237
Bsp13I TCCGGA 1 cut(s) 624
Bsp1407I TGTACA 1 cut(s) 549
Bsp143I GATC 1 cut(s) 1455
BspACI CCGC 2 cut(s) 21, 177
BspANI GGCC 1 cut(s) 844
BspCNI CTCAG 2 cut(s) 1032, 1150
BspEI TCCGGA 1 cut(s) 624
BspFNI CGCG 1 cut(s) 611
BspHI TCATGA 1 cut(s) 1329
BspLI GGNNCC 3 cut(s) 132, 744, 861
BspMAI CTGCAG 1 cut(s) 901
BspT107I GGYRCC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 549
BsrI ACTGG 3 cut(s) 234, 739, 1541
BssECI CCNNGG 3 cut(s) 126, 294, 1151
BssMI GATC 1 cut(s) 1455
Bst2UI CCWGG 2 cut(s) 128, 295
Bst4CI ACNGT 4 cut(s) 216, 332, 406, 1064
Bst6I CTCTTC 4 cut(s) 68, 993, 1047, 1238
BstAPI GCANNNNNTGC 3 cut(s) 137, 812, 1188
BstAUI TGTACA 1 cut(s) 549
BstBAI YACGTR 1 cut(s) 568
BstC8I GCNNGC 4 cut(s) 615, 842, 897, 1184
BstDEI CTNAG 3 cut(s) 358, 1040, 1137
BstF5I GGATG 3 cut(s) 587, 1134, 1213
BstFNI CGCG 1 cut(s) 611
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 1 cut(s) 1458
BstMAI GTCTC 1 cut(s) 692
BstMBI GATC 1 cut(s) 1455
BstMWI GCNNNNNNNGC 6 cut(s) 137, 310, 568, 803, 812, 1188
BstNI CCWGG 2 cut(s) 128, 295
BstNSI RCATGY 1 cut(s) 1186
BstSCI CCNGG 3 cut(s) 126, 293, 590
BstSFI CTRYAG 1 cut(s) 897
BstUI CGCG 1 cut(s) 611
BstV1I GCAGC 3 cut(s) 271, 818, 1303
BstV2I GAAGAC 2 cut(s) 1216, 1244
BstX2I RGATCY 1 cut(s) 1455
BstYI RGATCY 1 cut(s) 1455
BsuRI GGCC 1 cut(s) 844
BtsCI GGATG 3 cut(s) 587, 1134, 1213
BtsI GCAGTG 1 cut(s) 1090
BtsIMutI CAGTG 3 cut(s) 1090, 1389, 1548
Cac8I GCNNGC 4 cut(s) 615, 842, 897, 1184
CciI TCATGA 1 cut(s) 1329
CfoI GCGC 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 1282
Csp6I GTAC 4 cut(s) 550, 732, 945, 1060
CviAII CATG 9 cut(s) 35, 346, 385, 872, 1144, 1183, 1286, 1330, 1630
CviQI GTAC 4 cut(s) 550, 732, 945, 1060
DdeI CTNAG 3 cut(s) 358, 1040, 1137
DpnI GATC 1 cut(s) 1457
DpnII GATC 1 cut(s) 1455
DraI TTTAAA 1 cut(s) 1429
DraIII CACNNNGTG 1 cut(s) 497
Eam1104I CTCTTC 4 cut(s) 68, 993, 1047, 1238
EarI CTCTTC 4 cut(s) 68, 993, 1047, 1238
Ecl136II GAGCTC 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 1282
Eco53kI GAGCTC 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 1602
EcoICRI GAGCTC 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 126, 293
EcoT22I ATGCAT 2 cut(s) 315, 1573
EcoT38I GRGCYC 1 cut(s) 43
FaeI CATG 9 cut(s) 38, 349, 388, 875, 1147, 1186, 1289, 1333, 1633
FalI AAGNNNNNCTT 2 cut(s) 917, 949
FatI CATG 9 cut(s) 34, 345, 384, 871, 1143, 1182, 1285, 1329, 1629
Fnu4HI GCNGC 3 cut(s) 260, 807, 1317
FokI GGATG 3 cut(s) 574, 1141, 1220
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 3 cut(s) 260, 807, 1317
FspBI CTAG 7 cut(s) 180, 519, 750, 942, 986, 1110, 1196
GlaI GCGC 1 cut(s) 612
GluI GCNGC 3 cut(s) 260, 807, 1317
GsaI CCCAGC 2 cut(s) 266, 303
HaeIII GGCC 1 cut(s) 844
HapII CCGG 2 cut(s) 591, 625
HhaI GCGC 1 cut(s) 613
Hin1II CATG 9 cut(s) 38, 349, 388, 875, 1147, 1186, 1289, 1333, 1633
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HincII GTYRAC 1 cut(s) 1158
HindII GTYRAC 1 cut(s) 1158
HinfI GANTC 5 cut(s) 277, 515, 584, 635, 1556
HpaII CCGG 2 cut(s) 591, 625
HphI GGTGA 1 cut(s) 731
Hpy166II GTNNAC 3 cut(s) 550, 1088, 1158
Hpy188I TCNGA 2 cut(s) 226, 962
Hpy8I GTNNAC 3 cut(s) 550, 1088, 1158
HpyAV CCTTC 3 cut(s) 261, 574, 1069
HpyCH4III ACNGT 4 cut(s) 216, 332, 406, 1064
HpyCH4IV ACGT 1 cut(s) 567
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 310, 568, 803, 812, 1188
HpyF3I CTNAG 3 cut(s) 358, 1040, 1137
HpySE526I ACGT 1 cut(s) 567
Hsp92II CATG 9 cut(s) 38, 349, 388, 875, 1147, 1186, 1289, 1333, 1633
HspAI GCGC 1 cut(s) 611
Kpn2I TCCGGA 1 cut(s) 624
Kzo9I GATC 1 cut(s) 1455
LmnI GCTCC 3 cut(s) 38, 240, 973
Lsp1109I GCAGC 3 cut(s) 271, 818, 1303
LweI GCATC 3 cut(s) 400, 1119, 1483
MaeI CTAG 7 cut(s) 180, 519, 750, 942, 986, 1110, 1196
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 2 cut(s) 936, 1611
MalI GATC 1 cut(s) 1457
MboI GATC 1 cut(s) 1455
MboII GAAGA 8 cut(s) 45, 55, 69, 1010, 1064, 1216, 1244, 1255
MflI RGATCY 1 cut(s) 1455
MhlI GDGCHC 2 cut(s) 43, 237
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 3 cut(s) 883, 985, 1425
Mph1103I ATGCAT 2 cut(s) 315, 1573
MroI TCCGGA 1 cut(s) 624
MseI TTAA 4 cut(s) 8, 849, 1428, 1563
MslI CAYNNNNRTG 2 cut(s) 420, 1095
MspA1I CMGCKG 2 cut(s) 262, 809
MspI CCGG 2 cut(s) 591, 625
MspR9I CCNGG 3 cut(s) 128, 295, 592
Mva1269I GAATGC 1 cut(s) 1573
MvaI CCWGG 2 cut(s) 128, 295
MvnI CGCG 1 cut(s) 611
MwoI GCNNNNNNNGC 6 cut(s) 137, 310, 568, 803, 812, 1188
NciI CCSGG 1 cut(s) 592
NdeII GATC 1 cut(s) 1455
NlaIII CATG 9 cut(s) 38, 349, 388, 875, 1147, 1186, 1289, 1333, 1633
NlaIV GGNNCC 3 cut(s) 132, 744, 861
NmeAIII GCCGAG 1 cut(s) 1176
NsiI ATGCAT 2 cut(s) 315, 1573
NspI RCATGY 1 cut(s) 1186
OliI CACNNNNGTG 2 cut(s) 420, 1095
PaeI GCATGC 1 cut(s) 1186
PagI TCATGA 1 cut(s) 1329
PctI GAATGC 1 cut(s) 1573
PfeI GAWTC 4 cut(s) 515, 584, 635, 1556
PflFI GACNNNGTC 1 cut(s) 406
PflMI CCANNNNNTGG 1 cut(s) 1459
PkrI GCNGC 3 cut(s) 261, 808, 1318
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 568
PsiI TTATAA 4 cut(s) 255, 924, 956, 1167
Psp124BI GAGCTC 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 126, 293
PspFI CCCAGC 2 cut(s) 262, 299
PspGI CCWGG 2 cut(s) 126, 293
PspN4I GGNNCC 3 cut(s) 132, 744, 861
PspPI GGNCC 1 cut(s) 1282
PstI CTGCAG 1 cut(s) 901
PsuI RGATCY 1 cut(s) 1455
PsyI GACNNNGTC 1 cut(s) 406
PvuII CAGCTG 2 cut(s) 262, 809
RsaI GTAC 4 cut(s) 551, 733, 946, 1061
RsaNI GTAC 4 cut(s) 550, 732, 945, 1060
RseI CAYNNNNRTG 2 cut(s) 420, 1095
SacI GAGCTC 1 cut(s) 43
SaqAI TTAA 4 cut(s) 8, 849, 1428, 1563
SatI GCNGC 3 cut(s) 260, 807, 1317
Sau3AI GATC 1 cut(s) 1455
Sau96I GGNCC 1 cut(s) 1282
ScaI AGTACT 2 cut(s) 733, 946
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 3 cut(s) 128, 295, 592
SduI GDGCHC 2 cut(s) 43, 237
SfaNI GCATC 3 cut(s) 400, 1119, 1483
SfcI CTRYAG 1 cut(s) 897
SinI GGWCC 1 cut(s) 1282
SmiMI CAYNNNNRTG 2 cut(s) 420, 1095
SmlI CTYRAG 1 cut(s) 1015
SmoI CTYRAG 1 cut(s) 1015
SphI GCATGC 1 cut(s) 1186
SsiI CCGC 2 cut(s) 21, 177
SspMI CTAG 7 cut(s) 180, 519, 750, 942, 986, 1110, 1196
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 3 cut(s) 126, 293, 590
TaaI ACNGT 4 cut(s) 216, 332, 406, 1064
TaiI ACGT 1 cut(s) 570
TaqI TCGA 3 cut(s) 282, 436, 446
TatI WGTACW 3 cut(s) 549, 731, 944
TfiI GAWTC 4 cut(s) 515, 584, 635, 1556
Tru1I TTAA 4 cut(s) 8, 849, 1428, 1563
Tru9I TTAA 4 cut(s) 8, 849, 1428, 1563
TscAI CASTG 3 cut(s) 1097, 1389, 1548
TseI GCWGC 3 cut(s) 259, 806, 1316
TspDTI ATGAA 9 cut(s) 263, 334, 401, 576, 888, 1088, 1160, 1250, 1422
TspGWI ACGGA 1 cut(s) 1430
TspRI CASTG 3 cut(s) 1097, 1389, 1548
Tth111I GACNNNGTC 1 cut(s) 406
Van91I CCANNNNNTGG 1 cut(s) 1459
VpaK11BI GGWCC 1 cut(s) 1282
XapI RAATTY 4 cut(s) 82, 102, 683, 1201
XbaI TCTAGA 1 cut(s) 518
XceI RCATGY 1 cut(s) 1186
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XspI CTAG 7 cut(s) 180, 519, 750, 942, 986, 1110, 1196
ZrmI AGTACT 2 cut(s) 733, 946
Zsp2I ATGCAT 2 cut(s) 315, 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.