Rorug04G0084400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012605
Physical Location & Seq
Reverse (-)
12909821 .. 12911851
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0084400.1

Sequence Viewer

Length: 771 bp
ATGGTGAGAGCTCCTTGCTGCGAGAAAATGGGGTTGAAGAAGGGGCCATGGACTACTGAAGAAGACCAGATTCTCATCTCCTACATCCAAAATTACGGTCATGGAAATTGGCGAGCTCTGCCCAAGCATGCTGGCTTGTTGAGATGTGGGAAGAGCTGCAGACTCCGGTGGATAAACTATCTGCGGCCGGATATCAAGAGAGGAAACTTCACTAGAGAAGAAGAAGATGCCATCATCAATCTGCATGAAATGCTTGGTAACAGATGGTCAGCAATTGCTGCAAGATTACCAGGAAGAACTGATAATGAAATAAAAAATGTATGGCATACCCACTTGAAGAAGAGACTACTCAAACATAATAACACCACATCACCAACATGTAGCACTTCGAATCTCACAAGCCAATATGCGGAGCCAGAAGATGTGAATTTTGAGAGTCCAACAAGTCATGCATCAATGTCACCACAACCAAGTTCTAGTGATGATTTTTCATCAGTCACTGAAACCTCGGCCGCAACCGGTCAGAACATGATCAAAGATGAAAAGATCGAGTTCGATGCAACCGTCCTCGAAATCGATGACAGTTTCTGGTCAGAGGCACTTTCAGCTGACAATTGCGGCGACAACCCATTAGAGTTTTTGGAGGGCACAAATGATCAAGCACAACCCCAACTTCTACTCCCTTCAAACATTGATGATGGCATGGACTTTTGGTACGACCTTTTCATCAAAGCTGGCTATGGGGACACACTAGAACTACCAGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

256

Amino Acids

28.96

Weight (kDa)

4.91

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 6.7e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 7.4e-15 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 3.7e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 115 7e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 184, 410, 513, 618
AcoI YGGCCR 2 cut(s) 185, 510
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 1 cut(s) 716
AfiI CCNNNNNNNGG 1 cut(s) 409
AflIII ACRYGT 1 cut(s) 377
AgeI ACCGGT 1 cut(s) 518
AgsI TTSAA 3 cut(s) 37, 337, 687
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 5 cut(s) 11, 116, 156, 608, 734
AluI AGCT 5 cut(s) 11, 116, 156, 608, 734
Alw21I GWGCWC 2 cut(s) 13, 118
Alw26I GTCTC 1 cut(s) 337
AlwNI CAGNNNCTG 2 cut(s) 500, 588
AoxI GGCC 3 cut(s) 44, 185, 510
ApeKI GCWGC 3 cut(s) 18, 156, 278
ApoI RAATTY 1 cut(s) 427
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
AsiGI ACCGGT 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 3 cut(s) 16, 363, 453
AsuII TTCGAA 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 650
BanII GRGCYC 2 cut(s) 13, 118
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 13, 118
BbvI GCAGC 3 cut(s) 5, 143, 265
BccI CCATC 3 cut(s) 239, 258, 692
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 2 cut(s) 531, 655
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 3 cut(s) 213, 477, 752
BfmI CTRYAG 1 cut(s) 157
BisI GCNGC 6 cut(s) 19, 157, 185, 279, 513, 619
BlsI GCNGC 6 cut(s) 20, 158, 186, 280, 514, 620
Bme1390I CCNGG 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 45, 414
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 3 cut(s) 217, 461, 547
BpiI GAAGAC 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 389
Bsa29I ATCGAT 1 cut(s) 576
BsaBI GATNNNNATC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 47, 507
BsaWI WCCGGW 2 cut(s) 165, 518
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse118I RCCGGY 1 cut(s) 518
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 2 cut(s) 47, 507
BseGI GGATG 1 cut(s) 84
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 650
BseX3I CGGCCG 2 cut(s) 185, 510
BseXI GCAGC 3 cut(s) 5, 143, 265
Bsh1285I CGRYCG 2 cut(s) 188, 513
BshFI GGCC 3 cut(s) 46, 187, 512
BshTI ACCGGT 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 576
BsiEI CGRYCG 2 cut(s) 188, 513
BsiHKAI GWGCWC 2 cut(s) 13, 118
BsiSI CCGG 3 cut(s) 166, 188, 519
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 1 cut(s) 409
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 3 cut(s) 46, 187, 512
Bsp119I TTCGAA 1 cut(s) 389
Bsp1286I GDGCHC 3 cut(s) 13, 118, 650
Bsp143I GATC 3 cut(s) 531, 546, 655
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 4 cut(s) 184, 410, 513, 618
BspANI GGCC 3 cut(s) 46, 187, 512
BspDI ATCGAT 1 cut(s) 576
BspLI GGNNCC 2 cut(s) 45, 414
BspMAI CTGCAG 1 cut(s) 161
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 1 cut(s) 389
BsrFI RCCGGY 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 47, 507
BssMI GATC 3 cut(s) 531, 546, 655
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 98, 565, 584
Bst6I CTCTTC 2 cut(s) 146, 335
BstAPI GCANNNNNTGC 2 cut(s) 250, 278
BstBI TTCGAA 1 cut(s) 389
BstC8I GCNNGC 4 cut(s) 114, 129, 133, 736
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 84
BstKTI GATC 3 cut(s) 534, 549, 658
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 3 cut(s) 531, 546, 655
BstMCI CGRYCG 2 cut(s) 188, 513
BstMWI GCNNNNNNNGC 4 cut(s) 118, 250, 278, 605
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 2 cut(s) 131, 381
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 650
BstV1I GCAGC 3 cut(s) 5, 143, 265
BstV2I GAAGAC 1 cut(s) 69
BstZI CGGCCG 2 cut(s) 185, 510
Bsu15I ATCGAT 1 cut(s) 576
BsuRI GGCC 3 cut(s) 46, 187, 512
BsuTUI ATCGAT 1 cut(s) 576
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 4 cut(s) 114, 129, 133, 736
CaiI CAGNNNCTG 2 cut(s) 500, 588
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 44
ClaI ATCGAT 1 cut(s) 576
Csp6I GTAC 1 cut(s) 715
CspAI ACCGGT 1 cut(s) 518
CviAII CATG 8 cut(s) 48, 101, 128, 245, 378, 449, 529, 703
CviQI GTAC 1 cut(s) 715
DpnI GATC 3 cut(s) 533, 548, 657
DpnII GATC 3 cut(s) 531, 546, 655
EaeI YGGCCR 2 cut(s) 185, 510
EagI CGGCCG 2 cut(s) 185, 510
Eam1104I CTCTTC 2 cut(s) 146, 335
EarI CTCTTC 2 cut(s) 146, 335
Ecl136II GAGCTC 2 cut(s) 11, 116
EclXI CGGCCG 2 cut(s) 185, 510
Eco130I CCWWGG 1 cut(s) 47
Eco24I GRGCYC 2 cut(s) 13, 118
Eco32I GATATC 1 cut(s) 193
Eco52I CGGCCG 2 cut(s) 185, 510
Eco53kI GAGCTC 2 cut(s) 11, 116
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 2 cut(s) 11, 116
EcoRII CCWGG 1 cut(s) 289
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 47
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 2 cut(s) 13, 118
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 8 cut(s) 51, 104, 131, 248, 381, 452, 532, 706
FaqI GGGAC 1 cut(s) 758
FatI CATG 8 cut(s) 47, 100, 127, 244, 377, 448, 528, 702
FbaI TGATCA 2 cut(s) 531, 655
Fnu4HI GCNGC 6 cut(s) 19, 157, 185, 279, 513, 619
FokI GGATG 1 cut(s) 71
FriOI GRGCYC 2 cut(s) 13, 118
Fsp4HI GCNGC 6 cut(s) 19, 157, 185, 279, 513, 619
FspBI CTAG 3 cut(s) 213, 477, 752
GluI GCNGC 6 cut(s) 19, 157, 185, 279, 513, 619
HaeIII GGCC 3 cut(s) 46, 187, 512
HapII CCGG 3 cut(s) 166, 188, 519
Hin1II CATG 8 cut(s) 51, 104, 131, 248, 381, 452, 532, 706
HinfI GANTC 4 cut(s) 70, 162, 391, 436
HpaII CCGG 3 cut(s) 166, 188, 519
HphI GGTGA 3 cut(s) 16, 363, 453
Hpy188I TCNGA 2 cut(s) 525, 595
Hpy188III TCNNGA 1 cut(s) 196
HpyAV CCTTC 2 cut(s) 34, 693
HpyCH4III ACNGT 3 cut(s) 98, 565, 584
HpyCH4V TGCA 5 cut(s) 159, 244, 281, 452, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 250, 278, 605
Hsp92II CATG 8 cut(s) 51, 104, 131, 248, 381, 452, 532, 706
Ksp22I TGATCA 2 cut(s) 531, 655
Kzo9I GATC 3 cut(s) 531, 546, 655
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 2 cut(s) 16, 412
Lsp1109I GCAGC 3 cut(s) 5, 143, 265
LweI GCATC 3 cut(s) 217, 461, 547
MaeI CTAG 3 cut(s) 213, 477, 752
MaeIII GTNAC 3 cut(s) 257, 459, 496
MalI GATC 3 cut(s) 533, 548, 657
MboI GATC 3 cut(s) 531, 546, 655
MfeI CAATTG 2 cut(s) 273, 613
MhlI GDGCHC 3 cut(s) 13, 118, 650
MluCI AATT 5 cut(s) 91, 106, 273, 427, 613
MlyI GAGTC 2 cut(s) 156, 445
MmeI TCCRAC 1 cut(s) 464
MnlI CCTC 5 cut(s) 194, 517, 578, 589, 637
Mph1103I ATGCAT 1 cut(s) 454
MslI CAYNNNNRTG 1 cut(s) 376
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 3 cut(s) 166, 188, 519
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 2 cut(s) 273, 613
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 4 cut(s) 118, 250, 278, 605
NcoI CCATGG 1 cut(s) 47
NdeII GATC 3 cut(s) 531, 546, 655
NlaIII CATG 8 cut(s) 51, 104, 131, 248, 381, 452, 532, 706
NlaIV GGNNCC 2 cut(s) 45, 414
NmeAIII GCCGAG 1 cut(s) 488
NmuCI GTSAC 2 cut(s) 459, 496
NsiI ATGCAT 1 cut(s) 454
NspI RCATGY 2 cut(s) 131, 381
NspV TTCGAA 1 cut(s) 389
PaeI GCATGC 1 cut(s) 131
PciI ACATGT 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 561
PfeI GAWTC 2 cut(s) 70, 391
PinAI ACCGGT 1 cut(s) 518
PkrI GCNGC 6 cut(s) 20, 158, 186, 280, 514, 620
PleI GAGTC 2 cut(s) 156, 444
PpsI GAGTC 2 cut(s) 156, 444
PscI ACATGT 1 cut(s) 377
Psp124BI GAGCTC 2 cut(s) 13, 118
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 45, 414
PspPI GGNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 161
PstNI CAGNNNCTG 2 cut(s) 500, 588
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 1 cut(s) 716
RsaNI GTAC 1 cut(s) 715
RseI CAYNNNNRTG 1 cut(s) 376
SacI GAGCTC 2 cut(s) 13, 118
SapI GCTCTTC 1 cut(s) 146
SatI GCNGC 6 cut(s) 19, 157, 185, 279, 513, 619
Sau3AI GATC 3 cut(s) 531, 546, 655
Sau96I GGNCC 1 cut(s) 44
SchI GAGTC 2 cut(s) 156, 445
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 3 cut(s) 13, 118, 650
SetI ASST 7 cut(s) 13, 118, 158, 509, 610, 723, 736
SfaNI GCATC 3 cut(s) 217, 461, 547
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 376
SphI GCATGC 1 cut(s) 131
Sse9I AATT 5 cut(s) 91, 106, 273, 427, 613
SsiI CCGC 4 cut(s) 184, 410, 513, 618
SspMI CTAG 3 cut(s) 213, 477, 752
SstI GAGCTC 2 cut(s) 13, 118
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 3 cut(s) 98, 565, 584
TaqI TCGA 5 cut(s) 389, 549, 555, 570, 576
TasI AATT 5 cut(s) 91, 106, 273, 427, 613
TauI GCSGC 3 cut(s) 187, 515, 621
TfiI GAWTC 2 cut(s) 70, 391
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 2 cut(s) 459, 496
TseI GCWGC 3 cut(s) 18, 156, 278
Tsp45I GTSAC 2 cut(s) 459, 496
TspDTI ATGAA 5 cut(s) 261, 321, 480, 555, 715
TspRI CASTG 1 cut(s) 505
XapI RAATTY 1 cut(s) 427
XceI RCATGY 2 cut(s) 131, 381
XspI CTAG 3 cut(s) 213, 477, 752
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.