Rroxscaffold_5G00352720

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012605
Physical Location & Seq
Reverse (-)
29932992 .. 29937871
4880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352720.1

Sequence Viewer

Length: 1827 bp
ATGTTGTCTACATCCAAACATGGAGCTCTCACTCTTTCTTCAATTCTCTTCAAACATCTCACATCCCTTACAGCATTTTCACCATTCTCTTCAAAACCCAATTCAAATTTCACAGCCCCAAATCAGATTGCCACAATCCCAAAACAACCCGCTGCAAGAACAAGAGTCCCACAGGTTTCAGACCCAGTAAGTGCTTCTGTTGTCGATTCTGTATGTTCTTTACTTTCTAGAGAAACCGCCAGTGTTGATAGTTTGTTGAGTGGTTTCAAACACGAGTTGAGTTCAGAAGTGGTGCTTCAAGTTTTGATGAATTATAAGCAGCTGGGAAGGAGAAAGACATTGGACTTCTTTTCTTGGGCTGGGTTTCAAATGCAGTTTCAGTTTGATGACTGTGTGGTTGAGTACATGGCTGATTTCTTAGGTAGGAGGAAGCTATTTGATGACATGAAATGTCTTTTATTGACTGTGTCCTTGCACAGGGGTCGGGTTTCGTGTCGAACTGTTTCGACTTGTATTAGGTTTCTGGGTAGGCAGGGGAGGATTAAAGAAGCACTTTGCGTGTTTGAAGAAATGGAATCTAAATTTGGGTGTAAACCTGATAATCTTGTGTACAATAATGTGCTTTATGTGCTTTGTAAGAAGGAGTCATCTGGGCATTTGATTGATTTTGCGCTTGCAGTTTTTCGGAGAATTGAATCCCCTGATATGTATTCTTATAGTAATATGCTTGTTGGTTTGTGTAAATTTGGTAGATTTGAGACTGCTGTTGAAGTTTTTGGTGAAATGTGTGGGACTGGTTTGGTTCCTACTAGGTCTGCAGTTAATGTTCTTATTGGAGAGTTGTGTTTATTGAGTGCGAAAGAAGCAGCTGTTGCTAAAGTAAGAGTGAAAAATGTTCGTAGGCCGTTTAAGATTTTGGTTCCAAATGTGCATGAAAAGAGTGGTGCTATCCAGCCTGCACTTGGAGTGTTTTGGGAAGTTTATAAACTAGGCTTATTACCTAGTACTTTTGTCATAATCCGACTTCTATCAGAGCTTTGTCAATTGGGAAAAATGGAAGAAGCTGTTCGGGTCTTGAAGGCTGTTGAGGGTAGGAAGCTGAGTTGCGTGGAGGAGGGCTACTGTATTGTGATGAAGGGATTGTGTGAACACTGCTGCGTTGAGGAAGCTAGTCATTTGTTTGGGAGGATGCTCTCTCAGGGCATGAAGCCAAAGTTGACTATTTATAATTCTATTATTTGCATGCTATGCAAACTAGGGAATTTGGATGGTGCTGAAAGTGTCTTTAAGATAATGAACAAGAAGAGATGTCTTCCAGATAGTTGTACTTATTCTGCATTGGTCCATGCGTATTGTGAAGCTGGAAAATGGAAGGCTGCTTATGACTTAATGATAGAAATGCTCGGTTTGGGCTGCTCTGCGCCATTTCACACTTATAGCTTAGTGGATAAACTTTTGAGAGAAAATGAACAAACGGATTTGTGTTTTAAATTGGAAAGGAAGTTGGAAACCCAGATCTTGCAGAAGCTATGCAAAGATGGTCGATTAGAGGATGCGTATGAGAAGCTAAAGTCAATGATTGAAAAGGGGTTTTACCCTCCAGTGTATGCGAGAGATGCTTTTAAGAATGCATTTCGAAAGTATGGAAAATTAAATATAGCTCAGGATTTGTTACAGACGATAGATGACGTAGACGACAATGAGGAGAAACAACTTACCAATGAACCTATTGACAGAAGTATTTTCCACCGTCAACTGAAGAAGACAATGATGCAGACAAATGGAAGAGCGAGGAAAGATCAAACCAACAATGAAGTTGTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

608

Amino Acids

68.55

Weight (kDa)

8.82

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 213 9.9e-07 PPR repeat family
PPR_1 PF12854 233 - 262 7.7e-06 PPR repeat
PPR_2 PF13041 234 - 282 2e-07 PPR repeat family
PPR_long PF17177 386 - 471 2.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 395 - 451 2.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 401 - 432 5.5e-06 PPR repeat
PPR_2 PF13041 406 - 452 5.6e-13 PPR repeat family
PPR PF01535 408 - 437 1e-05 PPR repeat
PPR_1 PF12854 436 - 467 1.8e-08 PPR repeat
PPR_2 PF13041 442 - 480 2e-06 PPR repeat family
PPR PF01535 443 - 472 6.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 315, 984, 1227
AccI GTMKAC 2 cut(s) 8, 1692
AciI CCGC 2 cut(s) 150, 237
AcsI RAATTY 4 cut(s) 106, 581, 743, 1261
AcuI CTGAAG 1 cut(s) 1778
AfaI GTAC 4 cut(s) 404, 611, 1006, 1327
AfiI CCNNNNNNNGG 2 cut(s) 477, 962
AjuI GAANNNNNNNTTGG 4 cut(s) 92, 124, 567, 599
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 752
AoxI GGCC 1 cut(s) 902
ApeKI GCWGC 6 cut(s) 152, 319, 866, 1155, 1376, 1413
ApoI RAATTY 4 cut(s) 106, 581, 743, 1261
Asp700I GAANNNNTTC 2 cut(s) 502, 1065
AspLEI GCGC 2 cut(s) 673, 1423
AspS9I GGNCC 1 cut(s) 1342
AsuHPI GGTGA 2 cut(s) 72, 791
AsuII TTCGAA 1 cut(s) 1636
AvaII GGWCC 1 cut(s) 1342
BanII GRGCYC 1 cut(s) 28
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 1304, 1769
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 6 cut(s) 139, 331, 878, 1142, 1363, 1400
BccI CCATC 2 cut(s) 1262, 1532
BceAI ACGGC 1 cut(s) 889
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
BfmI CTRYAG 1 cut(s) 816
BglII AGATCT 1 cut(s) 1515
BisI GCNGC 6 cut(s) 153, 320, 867, 1156, 1377, 1414
BlsI GCNGC 6 cut(s) 154, 321, 868, 1157, 1378, 1415
BmcAI AGTACT 1 cut(s) 1006
Bme18I GGWCC 1 cut(s) 1342
BmgT120I GGNCC 1 cut(s) 1342
BmiI GGNNCC 2 cut(s) 804, 921
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 4 cut(s) 1179, 1543, 1606, 1761
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 1304, 1769
BpmI CTGGAG 1 cut(s) 1584
Bpu10I CCTNAGC 1 cut(s) 1662
Bpu14I TTCGAA 1 cut(s) 1636
Bsc4I CCNNNNNNNGG 2 cut(s) 477, 962
Bse1I ACTGG 4 cut(s) 185, 240, 799, 1601
BseGI GGATG 5 cut(s) 11, 62, 1194, 1273, 1558
BseLI CCNNNNNNNGG 2 cut(s) 477, 962
BseMII CTCAG 3 cut(s) 1091, 1211, 1676
BseNI ACTGG 4 cut(s) 185, 240, 799, 1601
BseRI GAGGAG 2 cut(s) 1127, 1718
BseXI GCAGC 6 cut(s) 139, 331, 878, 1142, 1363, 1400
BseYI CCCAGC 2 cut(s) 322, 359
BsgI GTGCAG 1 cut(s) 942
BshFI GGCC 1 cut(s) 904
BsiHKAI GWGCWC 1 cut(s) 28
BslFI GGGAC 2 cut(s) 152, 805
BslI CCNNNNNNNGG 2 cut(s) 477, 962
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 2 cut(s) 152, 805
BsmI GAATGC 1 cut(s) 1633
BsnI GGCC 1 cut(s) 904
Bsp119I TTCGAA 1 cut(s) 1636
Bsp1286I GDGCHC 1 cut(s) 28
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 2 cut(s) 1515, 1798
BspACI CCGC 2 cut(s) 150, 237
BspANI GGCC 1 cut(s) 904
BspCNI CTCAG 3 cut(s) 1092, 1210, 1675
BspLI GGNNCC 2 cut(s) 804, 921
BspMAI CTGCAG 1 cut(s) 820
BspQI GCTCTTC 1 cut(s) 1780
BspT104I TTCGAA 1 cut(s) 1636
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 4 cut(s) 185, 240, 799, 1601
BssMI GATC 2 cut(s) 1515, 1798
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst4CI ACNGT 5 cut(s) 392, 466, 502, 1124, 1751
Bst6I CTCTTC 4 cut(s) 53, 94, 1298, 1780
BstAPI GCANNNNNTGC 2 cut(s) 872, 1248
BstAUI TGTACA 1 cut(s) 609
BstBI TTCGAA 1 cut(s) 1636
BstC8I GCNNGC 3 cut(s) 675, 957, 1244
BstDEI CTNAG 5 cut(s) 418, 1100, 1197, 1441, 1662
BstENI CCTNNNNNAGG 1 cut(s) 475
BstF5I GGATG 5 cut(s) 11, 62, 1194, 1273, 1558
BstHHI GCGC 2 cut(s) 673, 1423
BstKTI GATC 2 cut(s) 1518, 1801
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 2 cut(s) 1515, 1798
BstMWI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
BstNSI RCATGY 1 cut(s) 1246
BstSFI CTRYAG 1 cut(s) 816
BstV1I GCAGC 6 cut(s) 139, 331, 878, 1142, 1363, 1400
BstV2I GAAGAC 2 cut(s) 1304, 1769
BstX2I RGATCY 1 cut(s) 1515
BstYI RGATCY 1 cut(s) 1515
BsuRI GGCC 1 cut(s) 904
BtsCI GGATG 5 cut(s) 11, 62, 1194, 1273, 1558
BtsI GCAGTG 1 cut(s) 1150
BtsIMutI CAGTG 3 cut(s) 247, 1150, 1608
Cac8I GCNNGC 3 cut(s) 675, 957, 1244
CfoI GCGC 2 cut(s) 673, 1423
Cfr13I GGNCC 1 cut(s) 1342
Csp6I GTAC 4 cut(s) 403, 610, 1005, 1326
CviAII CATG 7 cut(s) 20, 406, 445, 932, 1204, 1243, 1346
CviQI GTAC 4 cut(s) 403, 610, 1005, 1326
DdeI CTNAG 5 cut(s) 418, 1100, 1197, 1441, 1662
DpnI GATC 2 cut(s) 1517, 1800
DpnII GATC 2 cut(s) 1515, 1798
DraI TTTAAA 1 cut(s) 1489
Eam1104I CTCTTC 4 cut(s) 53, 94, 1298, 1780
EarI CTCTTC 4 cut(s) 53, 94, 1298, 1780
Ecl136II GAGCTC 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 1342
Eco53kI GAGCTC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 1778
EcoICRI GAGCTC 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 1633
EcoT38I GRGCYC 1 cut(s) 28
FaeI CATG 7 cut(s) 23, 409, 448, 935, 1207, 1246, 1349
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 537, 569
FaqI GGGAC 2 cut(s) 152, 805
FatI CATG 7 cut(s) 19, 405, 444, 931, 1203, 1242, 1345
FauI CCCGC 1 cut(s) 157
FblI GTMKAC 2 cut(s) 8, 1692
Fnu4HI GCNGC 6 cut(s) 153, 320, 867, 1156, 1377, 1414
FokI GGATG 4 cut(s) 49, 1201, 1280, 1565
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 6 cut(s) 153, 320, 867, 1156, 1377, 1414
FspBI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
GlaI GCGC 2 cut(s) 672, 1422
GluI GCNGC 6 cut(s) 153, 320, 867, 1156, 1377, 1414
GsaI CCCAGC 2 cut(s) 326, 363
GsuI CTGGAG 1 cut(s) 1584
HaeIII GGCC 1 cut(s) 904
HhaI GCGC 2 cut(s) 673, 1423
Hin1II CATG 7 cut(s) 23, 409, 448, 935, 1207, 1246, 1349
Hin6I GCGC 2 cut(s) 671, 1421
HinP1I GCGC 2 cut(s) 671, 1421
HincII GTYRAC 2 cut(s) 1218, 1754
HindII GTYRAC 2 cut(s) 1218, 1754
HinfI GANTC 5 cut(s) 165, 206, 575, 644, 695
HphI GGTGA 2 cut(s) 72, 791
Hpy166II GTNNAC 7 cut(s) 9, 593, 610, 1148, 1218, 1693, 1754
Hpy188I TCNGA 6 cut(s) 126, 181, 286, 687, 1022, 1033
Hpy188III TCNNGA 4 cut(s) 228, 1075, 1316, 1664
Hpy8I GTNNAC 7 cut(s) 9, 593, 610, 1148, 1218, 1693, 1754
HpyAV CCTTC 5 cut(s) 321, 634, 1072, 1129, 1366
HpyCH4III ACNGT 5 cut(s) 392, 466, 502, 1124, 1751
HpyCH4IV ACGT 1 cut(s) 1689
HpyF10VI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
HpyF3I CTNAG 5 cut(s) 418, 1100, 1197, 1441, 1662
HpySE526I ACGT 1 cut(s) 1689
Hsp92II CATG 7 cut(s) 23, 409, 448, 935, 1207, 1246, 1349
HspAI GCGC 2 cut(s) 671, 1421
Kzo9I GATC 2 cut(s) 1515, 1798
LguI GCTCTTC 1 cut(s) 1780
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 6 cut(s) 139, 331, 878, 1142, 1363, 1400
LweI GCATC 4 cut(s) 1179, 1543, 1606, 1761
MaeI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
MaeII ACGT 1 cut(s) 1689
MaeIII GTNAC 1 cut(s) 1671
MalI GATC 2 cut(s) 1517, 1800
MboI GATC 2 cut(s) 1515, 1798
MfeI CAATTG 1 cut(s) 1043
MflI RGATCY 1 cut(s) 1515
MhlI GDGCHC 1 cut(s) 28
MlyI GAGTC 2 cut(s) 174, 653
MmeI TCCRAC 2 cut(s) 1045, 1485
Mph1103I ATGCAT 1 cut(s) 1633
MroXI GAANNNNTTC 2 cut(s) 502, 1065
MseI TTAA 8 cut(s) 543, 822, 909, 1287, 1388, 1488, 1623, 1652
MspA1I CMGCKG 3 cut(s) 152, 322, 869
MunI CAATTG 1 cut(s) 1043
Mva1269I GAATGC 1 cut(s) 1633
MwoI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
NdeII GATC 2 cut(s) 1515, 1798
NlaIII CATG 7 cut(s) 23, 409, 448, 935, 1207, 1246, 1349
NlaIV GGNNCC 2 cut(s) 804, 921
NsiI ATGCAT 1 cut(s) 1633
NspI RCATGY 1 cut(s) 1246
NspV TTCGAA 1 cut(s) 1636
PaeI GCATGC 1 cut(s) 1246
PciSI GCTCTTC 1 cut(s) 1780
PctI GAATGC 1 cut(s) 1633
PdmI GAANNNNTTC 2 cut(s) 502, 1065
PfeI GAWTC 3 cut(s) 206, 575, 695
PflFI GACNNNGTC 1 cut(s) 466
PkrI GCNGC 6 cut(s) 154, 321, 868, 1157, 1378, 1415
PleI GAGTC 2 cut(s) 173, 652
PpsI GAGTC 2 cut(s) 173, 652
PsiI TTATAA 3 cut(s) 315, 984, 1227
Psp124BI GAGCTC 1 cut(s) 28
PspFI CCCAGC 2 cut(s) 322, 359
PspN4I GGNNCC 2 cut(s) 804, 921
PspPI GGNCC 1 cut(s) 1342
PstI CTGCAG 1 cut(s) 820
PsuI RGATCY 1 cut(s) 1515
PsyI GACNNNGTC 1 cut(s) 466
PvuII CAGCTG 2 cut(s) 322, 869
RsaI GTAC 4 cut(s) 404, 611, 1006, 1327
RsaNI GTAC 4 cut(s) 403, 610, 1005, 1326
SacI GAGCTC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 1780
SaqAI TTAA 8 cut(s) 543, 822, 909, 1287, 1388, 1488, 1623, 1652
SatI GCNGC 6 cut(s) 153, 320, 867, 1156, 1377, 1414
Sau3AI GATC 2 cut(s) 1515, 1798
Sau96I GGNCC 1 cut(s) 1342
ScaI AGTACT 1 cut(s) 1006
SchI GAGTC 2 cut(s) 174, 653
SduI GDGCHC 1 cut(s) 28
SfaNI GCATC 4 cut(s) 1179, 1543, 1606, 1761
SfcI CTRYAG 1 cut(s) 816
SfuI TTCGAA 1 cut(s) 1636
SinI GGWCC 1 cut(s) 1342
SphI GCATGC 1 cut(s) 1246
SsiI CCGC 2 cut(s) 150, 237
SspMI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
SstI GAGCTC 1 cut(s) 28
TaaI ACNGT 5 cut(s) 392, 466, 502, 1124, 1751
TaiI ACGT 1 cut(s) 1692
TaqI TCGA 5 cut(s) 204, 496, 506, 1543, 1636
TatI WGTACW 4 cut(s) 402, 609, 1004, 1325
TfiI GAWTC 3 cut(s) 206, 575, 695
Tru1I TTAA 8 cut(s) 543, 822, 909, 1287, 1388, 1488, 1623, 1652
Tru9I TTAA 8 cut(s) 543, 822, 909, 1287, 1388, 1488, 1623, 1652
TscAI CASTG 3 cut(s) 247, 1157, 1608
TseI GCWGC 6 cut(s) 152, 319, 866, 1155, 1376, 1413
TspDTI ATGAA 9 cut(s) 323, 461, 948, 1148, 1220, 1310, 1482, 1737, 1827
TspGWI ACGGA 1 cut(s) 1490
TspRI CASTG 3 cut(s) 247, 1157, 1608
Tth111I GACNNNGTC 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 1342
XagI CCTNNNNNAGG 1 cut(s) 475
XapI RAATTY 4 cut(s) 106, 581, 743, 1261
XbaI TCTAGA 1 cut(s) 227
XceI RCATGY 1 cut(s) 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 959
XmiI GTMKAC 2 cut(s) 8, 1692
XmnI GAANNNNTTC 2 cut(s) 502, 1065
XspI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
ZrmI AGTACT 1 cut(s) 1006
Zsp2I ATGCAT 1 cut(s) 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.