pycom13g28900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012605
Physical Location & Seq
Reverse (-)
25135382 .. 25137112
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g28900.1

Sequence Viewer

Length: 1731 bp
ATGCTCTCTCGCAGTAGAACCAAACATGGAGGACCTCTCATCCATTCGTCACTTCTCTTCAAATTCGTCACACATTTTTCTCCATTTTCTTCCAAACCCAATTCAAATTTCGCAACCCAGAATCAAATCGCTACAATCCCAAAAAAATTCACCACAAGAAGATCAATTCCACTCCCTGCAACAGTCCCACAAGCTCCAGACGCAGTAAGTTCTTCAATTGTCAGCTCAGTATGTTCTTTGCTCTCTAAAGATACCAAAGAAACCACAAGTATTGATATTTTGTTGAAAAAATTAAAAAAAAAGTTGAGTTCTGAACTTGTGCTGCAAATTTTGATGAATTATAAGCAATTGGGTAGGAGCAAGACATTGGATTTCTTCTCTTGGGCTGGATTTCAGATGGGTTTTCGGTTCGATGACTGTGTGGTTGAGTACATGGCTGATTTCTTAGGTAGGAGGAAGCTTTTTGATGATATGAAGTGTCTTCTGTTGACTGTGTCCTCCCACAAGGGTCGAGTTTCGTGTCGAGCTGTGTCCATTTGCATTAGGTTTCTGGGTAGGCAGGGGAGGATTAGAGAAGCCCTTTGTATGTTTGAAGAAATGGAATCAAAATTTGGTTGTAAACCTGATAATCTTGTGTACAATAATGTGCTCTACGTGCTTTGTAAGAAGGAATCGTCTGGCCATTTAATTGACTTTGCGCTTGCAGTTTTTCGGACAATTGAATCGCCTGATACTTATTCGTATAGTAATATTCTTGTTGGTTTATGTAAGTTTGGTAGATTTGAGACTGCCATTGAAGTTTTTGGTGATATGTGTAAGTCTGGTTTGGTTCCTACTCGGTCTGCAGTGAATGCTTTTATTGGAGAGTTGTGTTCATTCAGTGCGAAAGAAGGAGCTGTTAGGAAAGTAAGAGTGAGAAATTATTGTAGAACCTTTACGATTTTAGTTCCAAAAGTGGGTGGAAACAGTGATGCTATACAGCCTGCAGTTAGAGTTTTTTGGGCAGTGTATAAAGTGGGATTATTACCCAGTACATTTGCCATAATCCGGCTTCTTTCGGGGCTTTGTCAACTGGGAAAAGTGGAAGAGGCTGTTGAGATTTTGAAGGCTGTTGAGGGTAAGAAGCTGAGCTGTGTCGAAGAGGGCTACTGTATTGTGATGAAGGCTTTGTGTGAACACGGTCACGTTGAGGAAACTAGCCATATGTTTGGGAGGATGCTATCCCAAGGCATGAAACCAAAGTTGGCTGTTTATAATTCAATTATATGCATGCTTTGTAAACTAGGGAATTTGGATGATGCCGGAAGTGTCTTTAAGATGATGAACAAGAATAGATGTCTTCCGGATAGTATGACATATTCTGCGTTAATCCATGCTTATGGTGAAGCTAAAAATTGGGAAGCTTCTTATGGCTTATTGATAGAAATGCTTGGGTTGGGCTACTCTCCACATTTTCATACTTATAGATTAGTGGACAAAATTTTGAGGGAAAACGGGCAGATGGATATGTGTCTTAAGTTGGAAAGGAAGTTGGAAACACAGACGTTGCAGAAGCTCTGTAAAGATGACCAGTTAGAGGCTTCATATGAGAAGCTAAAATCAATGATTGGAAAGGGGTTTCACCCACCAGCATACGTGAGAGATTCTTTTGAAACTGCGTTCCGGAGGTATGGAAAATTGAAAATAGCTCAGGAATTGCTGCGGACGACAAGTGAAACTGACAGAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

577

Amino Acids

64.81

Weight (kDa)

9.27

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 180 - 222 3e-06 PPR repeat family
PPR_2 PF13041 243 - 291 3.5e-08 PPR repeat family
PPR_3 PF13812 406 - 462 4.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 410 - 441 7.2e-06 PPR repeat
PPR_2 PF13041 414 - 460 6.3e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 342, 1254
AccIII TCCGGA 2 cut(s) 1342, 1662
AciI CCGC 1 cut(s) 1702
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 7 cut(s) 62, 106, 146, 327, 608, 1288, 1479
AfaI GTAC 3 cut(s) 431, 638, 1033
AfiI CCNNNNNNNGG 3 cut(s) 956, 1047, 1576
AflII CTTAAG 1 cut(s) 1514
AjuI GAANNNNNNNTTGG 6 cut(s) 92, 124, 594, 626, 1226, 1258
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 1 cut(s) 779
Aor13HI TCCGGA 2 cut(s) 1342, 1662
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 2 cut(s) 322, 1699
ApoI RAATTY 7 cut(s) 62, 106, 146, 327, 608, 1288, 1479
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 1 cut(s) 32
AsuHPI GGTGA 4 cut(s) 142, 818, 1394, 1613
AvaII GGWCC 1 cut(s) 32
BalI TGGCCA 1 cut(s) 681
BbsI GAAGAC 2 cut(s) 473, 1331
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 2 cut(s) 309, 1686
BccI CCATC 2 cut(s) 391, 1495
BcoDI GTCTC 1 cut(s) 779
BfaI CTAG 2 cut(s) 1197, 1283
BfmI CTRYAG 2 cut(s) 843, 984
BfrI CTTAAG 1 cut(s) 1514
BisI GCNGC 2 cut(s) 323, 1700
BlpI GCTNAGC 1 cut(s) 1127
BlsI GCNGC 2 cut(s) 324, 1701
Bme18I GGWCC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 831
BmrI ACTGGG 2 cut(s) 1023, 1082
BmsI GCATC 3 cut(s) 961, 1206, 1288
BmuI ACTGGG 2 cut(s) 1023, 1082
BpiI GAAGAC 2 cut(s) 473, 1331
BplI GAGNNNNNCTC 2 cut(s) 21, 53
BpmI CTGGAG 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 1689
Bpu1102I GCTNAGC 1 cut(s) 1127
BsaAI YACGTR 2 cut(s) 655, 1636
BsaJI CCNNGG 1 cut(s) 1225
BsaWI WCCGGW 2 cut(s) 1342, 1662
Bsc4I CCNNNNNNNGG 3 cut(s) 956, 1047, 1576
Bse1I ACTGG 3 cut(s) 1029, 1077, 1570
BseAI TCCGGA 2 cut(s) 1342, 1662
BseDI CCNNGG 1 cut(s) 1225
BseGI GGATG 3 cut(s) 39, 1221, 1300
BseLI CCNNNNNNNGG 3 cut(s) 956, 1047, 1576
BseMII CTCAG 3 cut(s) 240, 1118, 1703
BseNI ACTGG 3 cut(s) 1029, 1077, 1570
BseXI GCAGC 2 cut(s) 309, 1686
BshFI GGCC 1 cut(s) 681
BsiHKAI GWGCWC 1 cut(s) 651
BsiSI CCGG 4 cut(s) 1048, 1302, 1343, 1663
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 956, 1047, 1576
BsmAI GTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 651
Bsp13I TCCGGA 2 cut(s) 1342, 1662
Bsp1407I TGTACA 1 cut(s) 636
Bsp143I GATC 1 cut(s) 161
Bsp1720I GCTNAGC 1 cut(s) 1127
BspACI CCGC 1 cut(s) 1702
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 3 cut(s) 239, 1119, 1702
BspEI TCCGGA 2 cut(s) 1342, 1662
BspLI GGNNCC 1 cut(s) 831
BspMAI CTGCAG 2 cut(s) 847, 988
BspTI CTTAAG 1 cut(s) 1514
BsrGI TGTACA 1 cut(s) 636
BsrI ACTGG 3 cut(s) 1029, 1077, 1570
BssECI CCNNGG 1 cut(s) 1225
BssMI GATC 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 1225
Bst4CI ACNGT 6 cut(s) 184, 419, 493, 968, 1151, 1181
Bst6I CTCTTC 3 cut(s) 62, 1080, 1134
BstAFI CTTAAG 1 cut(s) 1514
BstAPI GCANNNNNTGC 1 cut(s) 851
BstAUI TGTACA 1 cut(s) 636
BstBAI YACGTR 2 cut(s) 655, 1636
BstC8I GCNNGC 3 cut(s) 702, 984, 1271
BstDEI CTNAG 5 cut(s) 226, 445, 1127, 1689, 1728
BstF5I GGATG 3 cut(s) 39, 1221, 1300
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 1 cut(s) 164
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 1 cut(s) 161
BstMWI GCNNNNNNNGC 3 cut(s) 200, 655, 851
BstNSI RCATGY 1 cut(s) 1273
BstSFI CTRYAG 2 cut(s) 843, 984
BstV1I GCAGC 2 cut(s) 309, 1686
BstV2I GAAGAC 2 cut(s) 473, 1331
BstXI CCANNNNNNTGG 2 cut(s) 1208, 1379
BsuRI GGCC 1 cut(s) 681
BtsCI GGATG 3 cut(s) 39, 1221, 1300
BtsI GCAGTG 2 cut(s) 852, 1011
BtsIMutI CAGTG 4 cut(s) 852, 886, 973, 1011
Cac8I GCNNGC 3 cut(s) 702, 984, 1271
CfoI GCGC 1 cut(s) 700
Cfr13I GGNCC 1 cut(s) 32
CseI GACGC 1 cut(s) 209
Csp6I GTAC 3 cut(s) 430, 637, 1032
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 5 cut(s) 26, 433, 1231, 1270, 1373
CviQI GTAC 3 cut(s) 430, 637, 1032
DdeI CTNAG 5 cut(s) 226, 445, 1127, 1689, 1728
DpnI GATC 1 cut(s) 163
DpnII GATC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 3 cut(s) 62, 1080, 1134
EarI CTCTTC 3 cut(s) 62, 1080, 1134
Eco130I CCWWGG 1 cut(s) 1225
Eco47I GGWCC 1 cut(s) 32
EcoO109I RGGNCCY 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 1225
EcoT22I ATGCAT 1 cut(s) 1271
ErhI CCWWGG 1 cut(s) 1225
FaeI CATG 5 cut(s) 29, 436, 1234, 1273, 1376
FaqI GGGAC 1 cut(s) 170
FatI CATG 5 cut(s) 25, 432, 1230, 1269, 1372
FauNDI CATATG 2 cut(s) 1203, 1585
Fnu4HI GCNGC 2 cut(s) 323, 1700
FokI GGATG 3 cut(s) 26, 1228, 1307
Fsp4HI GCNGC 2 cut(s) 323, 1700
FspBI CTAG 2 cut(s) 1197, 1283
GlaI GCGC 1 cut(s) 699
GluI GCNGC 2 cut(s) 323, 1700
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 1 cut(s) 681
HapII CCGG 4 cut(s) 1048, 1302, 1343, 1663
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 700
Hin1II CATG 5 cut(s) 29, 436, 1234, 1273, 1376
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HincII GTYRAC 2 cut(s) 489, 1070
HindII GTYRAC 2 cut(s) 489, 1070
HindIII AAGCTT 2 cut(s) 458, 1401
HinfI GANTC 5 cut(s) 121, 602, 671, 722, 1643
HpaII CCGG 4 cut(s) 1048, 1302, 1343, 1663
HphI GGTGA 4 cut(s) 142, 818, 1394, 1613
Hpy166II GTNNAC 7 cut(s) 489, 620, 637, 1070, 1175, 1280, 1474
Hpy188I TCNGA 3 cut(s) 313, 396, 714
Hpy188III TCNNGA 4 cut(s) 197, 1343, 1663, 1691
Hpy8I GTNNAC 7 cut(s) 489, 620, 637, 1070, 1175, 1280, 1474
HpyAV CCTTC 4 cut(s) 661, 884, 1099, 1156
HpyCH4III ACNGT 6 cut(s) 184, 419, 493, 968, 1151, 1181
HpyCH4IV ACGT 4 cut(s) 654, 1185, 1544, 1635
HpyCH4V TGCA 8 cut(s) 179, 325, 540, 704, 845, 986, 1269, 1549
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 655, 851
HpyF3I CTNAG 5 cut(s) 226, 445, 1127, 1689, 1728
HpySE526I ACGT 4 cut(s) 654, 1185, 1544, 1635
Hsp92II CATG 5 cut(s) 29, 436, 1234, 1273, 1376
HspAI GCGC 1 cut(s) 698
Kpn2I TCCGGA 2 cut(s) 1342, 1662
Kzo9I GATC 1 cut(s) 161
LmnI GCTCC 3 cut(s) 199, 357, 893
Lsp1109I GCAGC 2 cut(s) 309, 1686
LweI GCATC 3 cut(s) 961, 1206, 1288
MaeI CTAG 2 cut(s) 1197, 1283
MaeII ACGT 4 cut(s) 654, 1185, 1544, 1635
MaeIII GTNAC 3 cut(s) 48, 67, 1181
MalI GATC 1 cut(s) 163
MboI GATC 1 cut(s) 161
MfeI CAATTG 3 cut(s) 216, 347, 717
MhlI GDGCHC 1 cut(s) 651
MlsI TGGCCA 1 cut(s) 681
MluNI TGGCCA 1 cut(s) 681
MmeI TCCRAC 2 cut(s) 1500, 1512
Mox20I TGGCCA 1 cut(s) 681
Mph1103I ATGCAT 1 cut(s) 1271
MroI TCCGGA 2 cut(s) 1342, 1662
MscI TGGCCA 1 cut(s) 681
MseI TTAA 5 cut(s) 293, 686, 1314, 1367, 1515
MslI CAYNNNNRTG 1 cut(s) 1377
Msp20I TGGCCA 1 cut(s) 681
MspCI CTTAAG 1 cut(s) 1514
MspI CCGG 4 cut(s) 1048, 1302, 1343, 1663
MunI CAATTG 3 cut(s) 216, 347, 717
Mva1269I GAATGC 1 cut(s) 856
MwoI GCNNNNNNNGC 3 cut(s) 200, 655, 851
NdeI CATATG 2 cut(s) 1203, 1585
NdeII GATC 1 cut(s) 161
NlaIII CATG 5 cut(s) 29, 436, 1234, 1273, 1376
NlaIV GGNNCC 1 cut(s) 831
NmuCI GTSAC 3 cut(s) 48, 67, 1181
NsiI ATGCAT 1 cut(s) 1271
NspI RCATGY 1 cut(s) 1273
PaeI GCATGC 1 cut(s) 1273
PctI GAATGC 1 cut(s) 856
PfeI GAWTC 5 cut(s) 121, 602, 671, 722, 1643
PflFI GACNNNGTC 1 cut(s) 493
PkrI GCNGC 2 cut(s) 324, 1701
Ppu21I YACGTR 2 cut(s) 655, 1636
PpuMI RGGWCCY 1 cut(s) 32
PsiI TTATAA 2 cut(s) 342, 1254
Psp5II RGGWCCY 1 cut(s) 32
PspN4I GGNNCC 1 cut(s) 831
PspPI GGNCC 1 cut(s) 32
PspPPI RGGWCCY 1 cut(s) 32
PstI CTGCAG 2 cut(s) 847, 988
PsyI GACNNNGTC 1 cut(s) 493
RsaI GTAC 3 cut(s) 431, 638, 1033
RsaNI GTAC 3 cut(s) 430, 637, 1032
RseI CAYNNNNRTG 1 cut(s) 1377
SaqAI TTAA 5 cut(s) 293, 686, 1314, 1367, 1515
SatI GCNGC 2 cut(s) 323, 1700
Sau3AI GATC 1 cut(s) 161
Sau96I GGNCC 1 cut(s) 32
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 3 cut(s) 961, 1206, 1288
SfcI CTRYAG 2 cut(s) 843, 984
SinI GGWCC 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 1377
SmlI CTYRAG 1 cut(s) 1514
SmoI CTYRAG 1 cut(s) 1514
SphI GCATGC 1 cut(s) 1273
SsiI CCGC 1 cut(s) 1702
SspI AATATT 1 cut(s) 751
SspMI CTAG 2 cut(s) 1197, 1283
StyI CCWWGG 1 cut(s) 1225
TaaI ACNGT 6 cut(s) 184, 419, 493, 968, 1151, 1181
TaiI ACGT 4 cut(s) 657, 1188, 1547, 1638
TaqI TCGA 4 cut(s) 411, 511, 523, 1137
TaqII GACCGA 1 cut(s) 828
TatI WGTACW 3 cut(s) 429, 636, 1031
TfiI GAWTC 5 cut(s) 121, 602, 671, 722, 1643
Tru1I TTAA 5 cut(s) 293, 686, 1314, 1367, 1515
Tru9I TTAA 5 cut(s) 293, 686, 1314, 1367, 1515
TscAI CASTG 4 cut(s) 852, 886, 973, 1011
TseFI GTSAC 3 cut(s) 48, 67, 1181
TseI GCWGC 2 cut(s) 322, 1699
Tsp45I GTSAC 3 cut(s) 48, 67, 1181
TspDTI ATGAA 8 cut(s) 350, 488, 864, 1175, 1247, 1337, 1445, 1572
TspRI CASTG 4 cut(s) 852, 886, 973, 1011
Tth111I GACNNNGTC 1 cut(s) 493
Vha464I CTTAAG 1 cut(s) 1514
VpaK11BI GGWCC 1 cut(s) 32
XapI RAATTY 7 cut(s) 62, 106, 146, 327, 608, 1288, 1479
XceI RCATGY 1 cut(s) 1273
XspI CTAG 2 cut(s) 1197, 1283
Zsp2I ATGCAT 1 cut(s) 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.