RLG00000008662

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012605
Physical Location & Seq
Forward (+)
34526073 .. 34528729
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008662

Sequence Viewer

Length: 1731 bp
ATGTTGTCTACATCTAAACATGGAGCTCTCACTCTTTCTTCAATTCTCTTCAAACATCTCACACCCCTTACAGCATTTTCACCATTCTCTTCAAAACCCAATTCAAATTTCACAACCACAAATCAGATTGCCACAATCCCAAAACAACCCGCTGCAAGAACAAGAGTCCCACAGGTTTCAGACCCAGTAAGTGCTTCTGTTGTCAATTCAGTATGTTCTTTACTTTCCAAAGAAACCGCCAGTGTTGATAGTTTGTTGAGTAGTTTCAAACACATGTTGAGTTCAGAAGTGGTGCTTCAAGTTCTGATGAATTATAAGGAGCTGGGAAGGAGAAAGACATTGGACTTCTTTTCTTGGGCTGGGTTTCAAATGCATTTTCAGTTTGATGACTGTGTGGTTGAGTACATGGCTGATTTCTTAGGTAGGAGGAAGCTCTTTGATGACATGAAATGTCTTTTATTGACTGTGTCCTTGCACAAGGGTCGGGTTTCGTGTCGAACAGTTTCGACTTGTATTAGGTTTCTAGGTAGGCAGGGGAGGATTAAAGAAGCACTTTGCGTGTTTGAAGAAATGGAATCTAAATTTGGGTGTAAACCTGATAATCTTGTGTACAATAATGTGCTTTACGTGCTTTGTAAGAAGGAGTCATCTGGGCATTTGATTGATTTTGCGCTTGCAGTTTTTCGGAGAATTGAATCGCCTGATATGTATTCGTATAGTAATATGCTTGTTGGTTTGTGTAAATTTGGTAGATTTGAGACTGCTGTTGAAGTTTTTGGTGAAATGTGTGGGACTGGTTTGGTTCCTACTAGGTCTGCGGTGAATGTTCTTATTGGAGAGTTGTGTTTATTGAGTGCGAAAGAAGCAGCTGTTGCTAAAGTAAGAGTGAAAAATGTTCGTAGGCCGTTTAAGATTTTGGTTCCAAATGTGCATGAAAAGAGTGGTGCTATCCAGCCTGCACTTGGAGTGTTTTGGGAAGTTTATAAACTAGGCTTATTACCTAGTACTTTTGTCATAATCCGACTTCTATCAGAGCTTTGTCAACTGGGAAGAATGGAAGAGGCTGTTCGGGTCTTGAAGGCTGTTGAGGGTAGGAAGCAGAGTTGCGTTGAGGAGGGCTACTGTATTGTGATGAAGGGATTGTGTGAACACTGCTGTGTTGAGGAAGCTAGTCATTTGTTTGGGAGGATGCTCTCTCAGGGCATGAAGCCAAAGTTGACTATTTATTATTCTATTATTTGCATGCTATGCAAACTAGGGAATTTGGATGGTGCTGAAAGTGTCTTTAAGATGATGAACAAGAAGAGATGTCTTCCGGATAGTTGTACTTATTCTGCATTGGTCCACGCTTATTGTAAAGCTGGAAAATGGAAAGCTGCTTATGACTTGGTGATAGAAATGCTCGGATTGGGCTGCTCTCCGCCTTTTCACACTTATAGCTTAGTGGATAAACTTTTGAGAGAAAATGAACAAACAGATTTGTGTTTTAAATTGGAAAGGAAGTTGGAAACCCAGATCTTGGAGAAGCTATGCAAAGATGGTCGATTAGAGGATGCGTATGAGAAGCTAAAGTCAATGATTGAAAAGGGGTTTTACCCACCAGTTTATGCGAGAGATGCTTTTAAGAATGCATTTCGGAAAAGTACTTTCCACCGTCAACTGAAGAAGACAATGATGCAGACAAATGGAAGAGCGAGGAAAGATCAAACCAGCAATGAAGTTGTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

577

Amino Acids

64.95

Weight (kDa)

9.13

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 213 9.2e-07 PPR repeat family
PPR_1 PF12854 233 - 262 7.2e-06 PPR repeat
PPR_2 PF13041 234 - 282 1.9e-07 PPR repeat family
PPR_long PF17177 387 - 469 6.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 395 - 451 1.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 407 - 453 2.9e-11 PPR repeat family
PPR_1 PF12854 436 - 467 1.4e-09 PPR repeat
PPR_2 PF13041 442 - 480 4.9e-08 PPR repeat family
PPR PF01535 443 - 472 9.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 315, 984
AccB7I CCANNNNNTGG 1 cut(s) 1519
AccI GTMKAC 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 1315
AciI CCGC 4 cut(s) 150, 237, 818, 1421
AcsI RAATTY 4 cut(s) 106, 581, 743, 1261
AcuI CTGAAG 1 cut(s) 1682
AfaI GTAC 5 cut(s) 404, 611, 1006, 1327, 1645
AfiI CCNNNNNNNGG 2 cut(s) 962, 1519
AflIII ACRYGT 1 cut(s) 273
AjuI GAANNNNNNNTTGG 4 cut(s) 92, 124, 567, 599
AleI CACNNNNGTG 1 cut(s) 1155
Alw21I GWGCWC 2 cut(s) 28, 1728
Alw26I GTCTC 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 1315
AoxI GGCC 1 cut(s) 902
ApeKI GCWGC 4 cut(s) 152, 866, 1376, 1413
ApoI RAATTY 4 cut(s) 106, 581, 743, 1261
Asp700I GAANNNNTTC 1 cut(s) 502
AspLEI GCGC 1 cut(s) 673
AspS9I GGNCC 1 cut(s) 1342
AsuHPI GGTGA 4 cut(s) 72, 791, 832, 1402
AvaII GGWCC 1 cut(s) 1342
BanII GRGCYC 1 cut(s) 28
BbsI GAAGAC 2 cut(s) 1304, 1673
Bbv12I GWGCWC 2 cut(s) 28, 1728
BbvI GCAGC 4 cut(s) 139, 878, 1363, 1400
BccI CCATC 2 cut(s) 1262, 1532
BceAI ACGGC 1 cut(s) 889
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 6 cut(s) 524, 810, 989, 1002, 1170, 1256
BglII AGATCT 1 cut(s) 1515
BisI GCNGC 4 cut(s) 153, 867, 1377, 1414
BlsI GCNGC 4 cut(s) 154, 868, 1378, 1415
BmcAI AGTACT 2 cut(s) 1006, 1645
Bme18I GGWCC 1 cut(s) 1342
BmgT120I GGNCC 1 cut(s) 1342
BmiI GGNNCC 2 cut(s) 804, 921
BmrI ACTGGG 2 cut(s) 179, 1055
BmsI GCATC 4 cut(s) 1179, 1543, 1606, 1665
BmuI ACTGGG 2 cut(s) 179, 1055
BpiI GAAGAC 2 cut(s) 1304, 1673
BsaAI YACGTR 1 cut(s) 628
BsaWI WCCGGW 1 cut(s) 1315
Bsc4I CCNNNNNNNGG 2 cut(s) 962, 1519
Bse1I ACTGG 5 cut(s) 185, 240, 799, 1050, 1601
Bse3DI GCAATG 1 cut(s) 1720
BseAI TCCGGA 1 cut(s) 1315
BseGI GGATG 3 cut(s) 1194, 1273, 1558
BseLI CCNNNNNNNGG 2 cut(s) 962, 1519
BseMI GCAATG 1 cut(s) 1720
BseMII CTCAG 1 cut(s) 1211
BseNI ACTGG 5 cut(s) 185, 240, 799, 1050, 1601
BseRI GAGGAG 1 cut(s) 1127
BseXI GCAGC 4 cut(s) 139, 878, 1363, 1400
BseYI CCCAGC 2 cut(s) 322, 359
BsgI GTGCAG 1 cut(s) 942
BshFI GGCC 1 cut(s) 904
BsiHKAI GWGCWC 2 cut(s) 28, 1728
BsiSI CCGG 1 cut(s) 1316
BslFI GGGAC 2 cut(s) 152, 805
BslI CCNNNNNNNGG 2 cut(s) 962, 1519
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 2 cut(s) 152, 805
BsmI GAATGC 1 cut(s) 1633
BsnI GGCC 1 cut(s) 904
Bsp1286I GDGCHC 2 cut(s) 28, 1728
Bsp13I TCCGGA 1 cut(s) 1315
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 2 cut(s) 1515, 1702
BspACI CCGC 4 cut(s) 150, 237, 818, 1421
BspANI GGCC 1 cut(s) 904
BspCNI CTCAG 1 cut(s) 1210
BspEI TCCGGA 1 cut(s) 1315
BspLI GGNNCC 2 cut(s) 804, 921
BspQI GCTCTTC 1 cut(s) 1684
BsrDI GCAATG 1 cut(s) 1720
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 5 cut(s) 185, 240, 799, 1050, 1601
BssMI GATC 2 cut(s) 1515, 1702
Bst4CI ACNGT 5 cut(s) 392, 466, 502, 1124, 1655
Bst6I CTCTTC 5 cut(s) 53, 94, 1053, 1298, 1684
BstAPI GCANNNNNTGC 2 cut(s) 872, 1248
BstAUI TGTACA 1 cut(s) 609
BstBAI YACGTR 1 cut(s) 628
BstC8I GCNNGC 3 cut(s) 675, 957, 1244
BstDEI CTNAG 3 cut(s) 418, 1197, 1441
BstF5I GGATG 3 cut(s) 1194, 1273, 1558
BstHHI GCGC 1 cut(s) 673
BstKTI GATC 2 cut(s) 1518, 1705
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 2 cut(s) 1515, 1702
BstMWI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
BstNSI RCATGY 2 cut(s) 277, 1246
BstV1I GCAGC 4 cut(s) 139, 878, 1363, 1400
BstV2I GAAGAC 2 cut(s) 1304, 1673
BstX2I RGATCY 1 cut(s) 1515
BstYI RGATCY 1 cut(s) 1515
BsuRI GGCC 1 cut(s) 904
BtsCI GGATG 3 cut(s) 1194, 1273, 1558
BtsI GCAGTG 1 cut(s) 1150
BtsIMutI CAGTG 2 cut(s) 247, 1150
Cac8I GCNNGC 3 cut(s) 675, 957, 1244
CfoI GCGC 1 cut(s) 673
Cfr13I GGNCC 1 cut(s) 1342
Csp6I GTAC 5 cut(s) 403, 610, 1005, 1326, 1644
CspCI CAANNNNNGTGG 2 cut(s) 1334, 1369
CviAII CATG 7 cut(s) 20, 274, 406, 445, 932, 1204, 1243
CviQI GTAC 5 cut(s) 403, 610, 1005, 1326, 1644
DdeI CTNAG 3 cut(s) 418, 1197, 1441
DpnI GATC 2 cut(s) 1517, 1704
DpnII GATC 2 cut(s) 1515, 1702
DraI TTTAAA 1 cut(s) 1489
Eam1104I CTCTTC 5 cut(s) 53, 94, 1053, 1298, 1684
EarI CTCTTC 5 cut(s) 53, 94, 1053, 1298, 1684
EciI GGCGGA 1 cut(s) 1410
Ecl136II GAGCTC 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 1342
Eco53kI GAGCTC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 1682
EcoICRI GAGCTC 1 cut(s) 26
EcoT22I ATGCAT 2 cut(s) 375, 1633
EcoT38I GRGCYC 1 cut(s) 28
FaeI CATG 7 cut(s) 23, 277, 409, 448, 935, 1207, 1246
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 537, 569
FaqI GGGAC 2 cut(s) 152, 805
FatI CATG 7 cut(s) 19, 273, 405, 444, 931, 1203, 1242
FauI CCCGC 1 cut(s) 157
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 4 cut(s) 153, 867, 1377, 1414
FokI GGATG 3 cut(s) 1201, 1280, 1565
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 4 cut(s) 153, 867, 1377, 1414
FspBI CTAG 6 cut(s) 524, 810, 989, 1002, 1170, 1256
GlaI GCGC 1 cut(s) 672
GluI GCNGC 4 cut(s) 153, 867, 1377, 1414
GsaI CCCAGC 2 cut(s) 326, 363
HaeIII GGCC 1 cut(s) 904
HapII CCGG 1 cut(s) 1316
HhaI GCGC 1 cut(s) 673
Hin1II CATG 7 cut(s) 23, 277, 409, 448, 935, 1207, 1246
Hin6I GCGC 1 cut(s) 671
HinP1I GCGC 1 cut(s) 671
HincII GTYRAC 3 cut(s) 1043, 1218, 1658
HindII GTYRAC 3 cut(s) 1043, 1218, 1658
HinfI GANTC 4 cut(s) 165, 575, 644, 695
HpaII CCGG 1 cut(s) 1316
HphI GGTGA 4 cut(s) 72, 791, 832, 1402
Hpy166II GTNNAC 8 cut(s) 9, 593, 610, 1043, 1148, 1218, 1345, 1658
Hpy188III TCNNGA 2 cut(s) 1075, 1316
Hpy8I GTNNAC 8 cut(s) 9, 593, 610, 1043, 1148, 1218, 1345, 1658
HpyAV CCTTC 4 cut(s) 321, 634, 1072, 1129
HpyCH4III ACNGT 5 cut(s) 392, 466, 502, 1124, 1655
HpyCH4IV ACGT 1 cut(s) 627
HpyF10VI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
HpyF3I CTNAG 3 cut(s) 418, 1197, 1441
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 7 cut(s) 23, 277, 409, 448, 935, 1207, 1246
HspAI GCGC 1 cut(s) 671
Kpn2I TCCGGA 1 cut(s) 1315
Kzo9I GATC 2 cut(s) 1515, 1702
LguI GCTCTTC 1 cut(s) 1684
LmnI GCTCC 2 cut(s) 23, 319
Lsp1109I GCAGC 4 cut(s) 139, 878, 1363, 1400
LweI GCATC 4 cut(s) 1179, 1543, 1606, 1665
MaeI CTAG 6 cut(s) 524, 810, 989, 1002, 1170, 1256
MaeII ACGT 1 cut(s) 627
MalI GATC 2 cut(s) 1517, 1704
MboI GATC 2 cut(s) 1515, 1702
MflI RGATCY 1 cut(s) 1515
MhlI GDGCHC 2 cut(s) 28, 1728
MlyI GAGTC 2 cut(s) 174, 653
MmeI TCCRAC 2 cut(s) 1045, 1485
Mph1103I ATGCAT 2 cut(s) 375, 1633
MroI TCCGGA 1 cut(s) 1315
MroXI GAANNNNTTC 1 cut(s) 502
MseI TTAA 5 cut(s) 543, 909, 1287, 1488, 1623
MslI CAYNNNNRTG 1 cut(s) 1155
MspA1I CMGCKG 2 cut(s) 152, 869
MspI CCGG 1 cut(s) 1316
Mva1269I GAATGC 1 cut(s) 1633
MwoI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
NdeII GATC 2 cut(s) 1515, 1702
NlaIII CATG 7 cut(s) 23, 277, 409, 448, 935, 1207, 1246
NlaIV GGNNCC 2 cut(s) 804, 921
NsiI ATGCAT 2 cut(s) 375, 1633
NspI RCATGY 2 cut(s) 277, 1246
OliI CACNNNNGTG 1 cut(s) 1155
PaeI GCATGC 1 cut(s) 1246
PciI ACATGT 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 1684
PctI GAATGC 1 cut(s) 1633
PdmI GAANNNNTTC 1 cut(s) 502
PfeI GAWTC 2 cut(s) 575, 695
PflFI GACNNNGTC 1 cut(s) 466
PflMI CCANNNNNTGG 1 cut(s) 1519
PkrI GCNGC 4 cut(s) 154, 868, 1378, 1415
PleI GAGTC 2 cut(s) 173, 652
PpsI GAGTC 2 cut(s) 173, 652
Ppu21I YACGTR 1 cut(s) 628
PscI ACATGT 1 cut(s) 273
PsiI TTATAA 2 cut(s) 315, 984
Psp124BI GAGCTC 1 cut(s) 28
PspFI CCCAGC 2 cut(s) 322, 359
PspN4I GGNNCC 2 cut(s) 804, 921
PspPI GGNCC 1 cut(s) 1342
PsuI RGATCY 1 cut(s) 1515
PsyI GACNNNGTC 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 5 cut(s) 404, 611, 1006, 1327, 1645
RsaNI GTAC 5 cut(s) 403, 610, 1005, 1326, 1644
RseI CAYNNNNRTG 1 cut(s) 1155
SacI GAGCTC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 1684
SaqAI TTAA 5 cut(s) 543, 909, 1287, 1488, 1623
SatI GCNGC 4 cut(s) 153, 867, 1377, 1414
Sau3AI GATC 2 cut(s) 1515, 1702
Sau96I GGNCC 1 cut(s) 1342
ScaI AGTACT 2 cut(s) 1006, 1645
SchI GAGTC 2 cut(s) 174, 653
SduI GDGCHC 2 cut(s) 28, 1728
SfaNI GCATC 4 cut(s) 1179, 1543, 1606, 1665
SinI GGWCC 1 cut(s) 1342
SmiMI CAYNNNNRTG 1 cut(s) 1155
SphI GCATGC 1 cut(s) 1246
SsiI CCGC 4 cut(s) 150, 237, 818, 1421
SspMI CTAG 6 cut(s) 524, 810, 989, 1002, 1170, 1256
SstI GAGCTC 1 cut(s) 28
TaaI ACNGT 5 cut(s) 392, 466, 502, 1124, 1655
TaiI ACGT 1 cut(s) 630
TaqI TCGA 3 cut(s) 496, 506, 1543
TatI WGTACW 5 cut(s) 402, 609, 1004, 1325, 1643
TfiI GAWTC 2 cut(s) 575, 695
Tru1I TTAA 5 cut(s) 543, 909, 1287, 1488, 1623
Tru9I TTAA 5 cut(s) 543, 909, 1287, 1488, 1623
TscAI CASTG 2 cut(s) 247, 1157
TseI GCWGC 4 cut(s) 152, 866, 1376, 1413
TspDTI ATGAA 8 cut(s) 323, 461, 948, 1148, 1220, 1310, 1482, 1731
TspRI CASTG 2 cut(s) 247, 1157
Tth111I GACNNNGTC 1 cut(s) 466
Van91I CCANNNNNTGG 1 cut(s) 1519
VpaK11BI GGWCC 1 cut(s) 1342
XapI RAATTY 4 cut(s) 106, 581, 743, 1261
XceI RCATGY 2 cut(s) 277, 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 959
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 502
XspI CTAG 6 cut(s) 524, 810, 989, 1002, 1170, 1256
ZrmI AGTACT 2 cut(s) 1006, 1645
Zsp2I ATGCAT 2 cut(s) 375, 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.