Rw4G012180

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012605
Physical Location & Seq
Reverse (-)
28630466 .. 28635396
4931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012180.1

Sequence Viewer

Length: 1728 bp
ATGTTGTCTACATCTAAACATGGAGCTCTCACTCTTTCTTCAATTCTCTTCAAACATCTCACATCCCTTGCAGCATTTTCACCATTCTCTTCAAAACCCAATTCAAATTTCACAACCCCCAATCAGATTTCCACAATCCCAAAACAACCCGCTGCAAGAGCAAGAGTCCCACAGGTTTCAGACCCAGTAAGTGCTTCTGTTGTCGATTCTGTATGTTCTTTACTTTCTAGAGAAACCGCCAGTGTTGATAGTTTGTTGAGTGGTTTCAAACACGAGTTGAGTTCAGAAGTGGTGCTTCAAGTTCTGATGAATTATAAGCAGCTGGGAAGGAGAAAGACATTGGACTTCTTTTCTTGGGCTGGGTTTCAAATGCATTTTCAGTTTGATGACTGTGTGGTTGAGTACATGGCTGATTTCTTAGGTAGGAGGAAGCTATTTGATGACATGAAGTGTCTTTTATTGACTGTGTCCTTGCACAAGGGTCGGGTTTCGTGTCGAACTGTTTCGACTTGTATTAGGTTTCTGGGTAGGCAGGGGAGGATTAAAGAAGCAGTTTGCGTGTTTGAAGAAATGGAATCTAAATTTGGGTGTAAACCTGATAATCTTGTGTACAATAATGTGCTTTACGTGCTTTGTAAGAAGGAGTCATCTGGGCATTTGATTGATTTTGCGCTTGCAGTTTTTCGGAGAATTGAGTCCCCTGATATGTATTCGTATAGTAACATGCTTGTTGGTTTGTGTAAATTTGGTAGGTTTGAGACTGCTGTTGAAGTTTTTGGTGAAATGTGTGGGACTGGTTTGGTTCCTACTAGGTCTGCAGTTAATGTTCTTATTGGAGAGTTGTGTTTATTAAGTGCGAAAGAAGCAGCTGTTGCTAAAGTAAGAGTGAAAAATGTTCGTAGGCCGTTTAAGATTTTGGTTCCAAATGTGCATGAAAAGAGCGGTGCTATGCAGCCTGCACTTGGAGTGTTTTGGGAAGTTTATAAACTAGGCTTATTACCTAGTACTTTTGTCATAATCCGACTTCTATCAGAGCTTTGTCAACTGGGAAAAATGGAAGAGGCTGTTCGGGTCTTGAAGGCTGTTGTGGGTAGGAAGCTGAGTTGCGTTGAGGAGGGCTACTGTATTGTGATGAAGGGATTGTGTGAACACTGCTGTGTTGAGGAGGCTAGTCATTTGTATGGGAGGATGCTCTCTCAGGGCATGAAGCCAAAGTTGAGTATTTATAATTCTATTATTTGCATGCTATGCAAACTAGGAAATTTGGATGGTGCTGAAAGTGTCTTTAAGATAATGAACAAGAAGAGATGTCTTCCGGATAGTTGTACTTATTCTGCATTGGTCCATGCGTATTGTGAAGCTGGAAAATGGAAAGCTGCTTATGACTTAATGATAGAAATGCTCGGTTTGGGCTGCTCTGTGCCATTTCACACTTATAGCTTAGTGGATAAACTTTTGAGAGAAAATGAACAAACGGATTTGTGTTTTAAATTAGAAAGGAAGTTGGAAACCCAGATCTTGGAGAAGCTATGCAAAGATGGTCGATTAGAGGATGCGTATGAGAAGCTAAAGTCAATGATTGAAAAGAGGTTTTACCCACCAGTTTATGCGAGAGATGCTTTTAAGAATGCATTTCGGAAGTATGGAAAATTAAAAATAGCTCAGGATTTGTTACAGACGATAGATGACTTAGACAACAATGAGGAGAAACAACTTAGCTGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

575

Amino Acids

64.72

Weight (kDa)

8.8

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 213 1.1e-06 PPR repeat family
PPR_1 PF12854 233 - 262 7.2e-06 PPR repeat
PPR_2 PF13041 234 - 282 1.9e-07 PPR repeat family
PPR_long PF17177 387 - 472 4.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 397 - 451 4.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 401 - 432 3.8e-06 PPR repeat
PPR_2 PF13041 406 - 452 5e-13 PPR repeat family
PPR PF01535 408 - 437 9.1e-06 PPR repeat
PPR_1 PF12854 436 - 467 1.7e-08 PPR repeat
PPR_2 PF13041 442 - 480 1.5e-06 PPR repeat family
PPR PF01535 443 - 472 6.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 315, 984, 1227
AccB7I CCANNNNNTGG 1 cut(s) 1519
AccBSI CCGCTC 1 cut(s) 942
AccI GTMKAC 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 1315
AciI CCGC 3 cut(s) 150, 237, 942
AcsI RAATTY 4 cut(s) 106, 581, 743, 1261
AfaI GTAC 4 cut(s) 404, 611, 1006, 1327
AfiI CCNNNNNNNGG 2 cut(s) 962, 1519
AjuI GAANNNNNNNTTGG 4 cut(s) 92, 124, 567, 599
AleI CACNNNNGTG 1 cut(s) 1155
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 1315
AoxI GGCC 1 cut(s) 902
ApeKI GCWGC 7 cut(s) 71, 152, 319, 866, 952, 1376, 1413
ApoI RAATTY 4 cut(s) 106, 581, 743, 1261
Asp700I GAANNNNTTC 1 cut(s) 502
AspLEI GCGC 1 cut(s) 673
AspS9I GGNCC 1 cut(s) 1342
AsuHPI GGTGA 2 cut(s) 72, 791
AvaII GGWCC 1 cut(s) 1342
BanII GRGCYC 1 cut(s) 28
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 1304
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 7 cut(s) 83, 139, 331, 878, 964, 1363, 1400
BccI CCATC 2 cut(s) 1262, 1532
BceAI ACGGC 1 cut(s) 889
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
BfmI CTRYAG 1 cut(s) 816
BglII AGATCT 1 cut(s) 1515
BisI GCNGC 7 cut(s) 72, 153, 320, 867, 953, 1377, 1414
BlsI GCNGC 7 cut(s) 73, 154, 321, 868, 954, 1378, 1415
BmcAI AGTACT 1 cut(s) 1006
Bme18I GGWCC 1 cut(s) 1342
BmgT120I GGNCC 1 cut(s) 1342
BmiI GGNNCC 2 cut(s) 804, 921
BmrI ACTGGG 2 cut(s) 179, 1055
BmsI GCATC 3 cut(s) 1179, 1543, 1606
BmuI ACTGGG 2 cut(s) 179, 1055
BpiI GAAGAC 1 cut(s) 1304
Bpu10I CCTNAGC 1 cut(s) 1662
BsaAI YACGTR 1 cut(s) 628
BsaWI WCCGGW 1 cut(s) 1315
Bsc4I CCNNNNNNNGG 2 cut(s) 962, 1519
Bse1I ACTGG 5 cut(s) 185, 240, 799, 1050, 1601
BseAI TCCGGA 1 cut(s) 1315
BseGI GGATG 4 cut(s) 62, 1194, 1273, 1558
BseLI CCNNNNNNNGG 2 cut(s) 962, 1519
BseMII CTCAG 3 cut(s) 1091, 1211, 1676
BseNI ACTGG 5 cut(s) 185, 240, 799, 1050, 1601
BseRI GAGGAG 3 cut(s) 1127, 1178, 1718
BseXI GCAGC 7 cut(s) 83, 139, 331, 878, 964, 1363, 1400
BseYI CCCAGC 2 cut(s) 322, 359
BsgI GTGCAG 1 cut(s) 942
BshFI GGCC 1 cut(s) 904
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 1316
BslFI GGGAC 3 cut(s) 152, 682, 805
BslI CCNNNNNNNGG 2 cut(s) 962, 1519
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 3 cut(s) 152, 682, 805
BsmI GAATGC 1 cut(s) 1633
BsnI GGCC 1 cut(s) 904
Bsp1286I GDGCHC 1 cut(s) 28
Bsp13I TCCGGA 1 cut(s) 1315
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 1 cut(s) 1515
BspACI CCGC 3 cut(s) 150, 237, 942
BspANI GGCC 1 cut(s) 904
BspCNI CTCAG 3 cut(s) 1092, 1210, 1675
BspEI TCCGGA 1 cut(s) 1315
BspLI GGNNCC 2 cut(s) 804, 921
BspMAI CTGCAG 1 cut(s) 820
BsrBI CCGCTC 1 cut(s) 942
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 5 cut(s) 185, 240, 799, 1050, 1601
BssMI GATC 1 cut(s) 1515
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 392, 466, 502, 1124
Bst6I CTCTTC 4 cut(s) 53, 94, 1053, 1298
BstAPI GCANNNNNTGC 2 cut(s) 872, 1248
BstAUI TGTACA 1 cut(s) 609
BstBAI YACGTR 1 cut(s) 628
BstC8I GCNNGC 3 cut(s) 675, 957, 1244
BstDEI CTNAG 7 cut(s) 418, 1100, 1197, 1441, 1662, 1690, 1715
BstF5I GGATG 4 cut(s) 62, 1194, 1273, 1558
BstHHI GCGC 1 cut(s) 673
BstKTI GATC 1 cut(s) 1518
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 1 cut(s) 1515
BstMWI GCNNNNNNNGC 6 cut(s) 158, 628, 863, 872, 1248, 1616
BstNSI RCATGY 2 cut(s) 727, 1246
BstSFI CTRYAG 1 cut(s) 816
BstV1I GCAGC 7 cut(s) 83, 139, 331, 878, 964, 1363, 1400
BstV2I GAAGAC 1 cut(s) 1304
BstX2I RGATCY 1 cut(s) 1515
BstYI RGATCY 1 cut(s) 1515
BsuRI GGCC 1 cut(s) 904
BtsCI GGATG 4 cut(s) 62, 1194, 1273, 1558
BtsI GCAGTG 1 cut(s) 1150
BtsIMutI CAGTG 2 cut(s) 247, 1150
Cac8I GCNNGC 3 cut(s) 675, 957, 1244
CfoI GCGC 1 cut(s) 673
Cfr13I GGNCC 1 cut(s) 1342
Csp6I GTAC 4 cut(s) 403, 610, 1005, 1326
CviAII CATG 8 cut(s) 20, 406, 445, 724, 932, 1204, 1243, 1346
CviQI GTAC 4 cut(s) 403, 610, 1005, 1326
DdeI CTNAG 7 cut(s) 418, 1100, 1197, 1441, 1662, 1690, 1715
DpnI GATC 1 cut(s) 1517
DpnII GATC 1 cut(s) 1515
DraI TTTAAA 1 cut(s) 1489
Eam1104I CTCTTC 4 cut(s) 53, 94, 1053, 1298
EarI CTCTTC 4 cut(s) 53, 94, 1053, 1298
Ecl136II GAGCTC 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 1342
Eco53kI GAGCTC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 26
EcoT22I ATGCAT 2 cut(s) 375, 1633
EcoT38I GRGCYC 1 cut(s) 28
FaeI CATG 8 cut(s) 23, 409, 448, 727, 935, 1207, 1246, 1349
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 3 cut(s) 152, 682, 805
FatI CATG 8 cut(s) 19, 405, 444, 723, 931, 1203, 1242, 1345
FauI CCCGC 1 cut(s) 157
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 7 cut(s) 72, 153, 320, 867, 953, 1377, 1414
FokI GGATG 4 cut(s) 49, 1201, 1280, 1565
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 7 cut(s) 72, 153, 320, 867, 953, 1377, 1414
FspBI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
GlaI GCGC 1 cut(s) 672
GluI GCNGC 7 cut(s) 72, 153, 320, 867, 953, 1377, 1414
GsaI CCCAGC 2 cut(s) 326, 363
HaeIII GGCC 1 cut(s) 904
HapII CCGG 1 cut(s) 1316
HhaI GCGC 1 cut(s) 673
Hin1II CATG 8 cut(s) 23, 409, 448, 727, 935, 1207, 1246, 1349
Hin6I GCGC 1 cut(s) 671
HinP1I GCGC 1 cut(s) 671
HincII GTYRAC 1 cut(s) 1043
HindII GTYRAC 1 cut(s) 1043
HinfI GANTC 5 cut(s) 165, 206, 575, 644, 695
HpaII CCGG 1 cut(s) 1316
HphI GGTGA 2 cut(s) 72, 791
Hpy166II GTNNAC 5 cut(s) 9, 593, 610, 1043, 1148
Hpy188I TCNGA 8 cut(s) 126, 181, 286, 306, 687, 1022, 1033, 1638
Hpy188III TCNNGA 4 cut(s) 228, 1075, 1316, 1664
Hpy8I GTNNAC 5 cut(s) 9, 593, 610, 1043, 1148
HpyAV CCTTC 4 cut(s) 321, 634, 1072, 1129
HpyCH4III ACNGT 4 cut(s) 392, 466, 502, 1124
HpyCH4IV ACGT 1 cut(s) 627
HpyF10VI GCNNNNNNNGC 6 cut(s) 158, 628, 863, 872, 1248, 1616
HpyF3I CTNAG 7 cut(s) 418, 1100, 1197, 1441, 1662, 1690, 1715
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 8 cut(s) 23, 409, 448, 727, 935, 1207, 1246, 1349
HspAI GCGC 1 cut(s) 671
Kpn2I TCCGGA 1 cut(s) 1315
Kzo9I GATC 1 cut(s) 1515
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 7 cut(s) 83, 139, 331, 878, 964, 1363, 1400
LweI GCATC 3 cut(s) 1179, 1543, 1606
MaeI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 2 cut(s) 719, 1671
MalI GATC 1 cut(s) 1517
MbiI CCGCTC 1 cut(s) 942
MboI GATC 1 cut(s) 1515
MboII GAAGA 7 cut(s) 30, 40, 81, 578, 1070, 1304, 1315
MflI RGATCY 1 cut(s) 1515
MhlI GDGCHC 1 cut(s) 28
MlyI GAGTC 3 cut(s) 174, 653, 704
MmeI TCCRAC 2 cut(s) 1045, 1485
Mph1103I ATGCAT 2 cut(s) 375, 1633
MroI TCCGGA 1 cut(s) 1315
MroXI GAANNNNTTC 1 cut(s) 502
MseI TTAA 9 cut(s) 543, 822, 851, 909, 1287, 1388, 1488, 1623, 1652
MslI CAYNNNNRTG 2 cut(s) 1155, 1179
MspA1I CMGCKG 3 cut(s) 152, 322, 869
MspI CCGG 1 cut(s) 1316
Mva1269I GAATGC 1 cut(s) 1633
MwoI GCNNNNNNNGC 6 cut(s) 158, 628, 863, 872, 1248, 1616
NdeII GATC 1 cut(s) 1515
NlaIII CATG 8 cut(s) 23, 409, 448, 727, 935, 1207, 1246, 1349
NlaIV GGNNCC 2 cut(s) 804, 921
NsiI ATGCAT 2 cut(s) 375, 1633
NspI RCATGY 2 cut(s) 727, 1246
OliI CACNNNNGTG 1 cut(s) 1155
PaeI GCATGC 1 cut(s) 1246
PctI GAATGC 1 cut(s) 1633
PdmI GAANNNNTTC 1 cut(s) 502
PfeI GAWTC 2 cut(s) 206, 575
PflFI GACNNNGTC 1 cut(s) 466
PflMI CCANNNNNTGG 1 cut(s) 1519
PkrI GCNGC 7 cut(s) 73, 154, 321, 868, 954, 1378, 1415
PleI GAGTC 3 cut(s) 173, 652, 703
PpsI GAGTC 3 cut(s) 173, 652, 703
Ppu21I YACGTR 1 cut(s) 628
PsiI TTATAA 3 cut(s) 315, 984, 1227
Psp124BI GAGCTC 1 cut(s) 28
PspFI CCCAGC 2 cut(s) 322, 359
PspN4I GGNNCC 2 cut(s) 804, 921
PspPI GGNCC 1 cut(s) 1342
PstI CTGCAG 1 cut(s) 820
PsuI RGATCY 1 cut(s) 1515
PsyI GACNNNGTC 1 cut(s) 466
PvuII CAGCTG 2 cut(s) 322, 869
RsaI GTAC 4 cut(s) 404, 611, 1006, 1327
RsaNI GTAC 4 cut(s) 403, 610, 1005, 1326
RseI CAYNNNNRTG 2 cut(s) 1155, 1179
SacI GAGCTC 1 cut(s) 28
SaqAI TTAA 9 cut(s) 543, 822, 851, 909, 1287, 1388, 1488, 1623, 1652
SatI GCNGC 7 cut(s) 72, 153, 320, 867, 953, 1377, 1414
Sau3AI GATC 1 cut(s) 1515
Sau96I GGNCC 1 cut(s) 1342
ScaI AGTACT 1 cut(s) 1006
SchI GAGTC 3 cut(s) 174, 653, 704
SduI GDGCHC 1 cut(s) 28
SfaNI GCATC 3 cut(s) 1179, 1543, 1606
SfcI CTRYAG 1 cut(s) 816
SinI GGWCC 1 cut(s) 1342
SmiMI CAYNNNNRTG 2 cut(s) 1155, 1179
SphI GCATGC 1 cut(s) 1246
SsiI CCGC 3 cut(s) 150, 237, 942
SspMI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
SstI GAGCTC 1 cut(s) 28
TaaI ACNGT 4 cut(s) 392, 466, 502, 1124
TaiI ACGT 1 cut(s) 630
TaqI TCGA 4 cut(s) 204, 496, 506, 1543
TatI WGTACW 4 cut(s) 402, 609, 1004, 1325
TfiI GAWTC 2 cut(s) 206, 575
Tru1I TTAA 9 cut(s) 543, 822, 851, 909, 1287, 1388, 1488, 1623, 1652
Tru9I TTAA 9 cut(s) 543, 822, 851, 909, 1287, 1388, 1488, 1623, 1652
TscAI CASTG 2 cut(s) 247, 1157
TseI GCWGC 7 cut(s) 71, 152, 319, 866, 952, 1376, 1413
TspDTI ATGAA 7 cut(s) 323, 461, 948, 1148, 1220, 1310, 1482
TspGWI ACGGA 1 cut(s) 1490
TspRI CASTG 2 cut(s) 247, 1157
Tth111I GACNNNGTC 1 cut(s) 466
Van91I CCANNNNNTGG 1 cut(s) 1519
VpaK11BI GGWCC 1 cut(s) 1342
XapI RAATTY 4 cut(s) 106, 581, 743, 1261
XbaI TCTAGA 1 cut(s) 227
XceI RCATGY 2 cut(s) 727, 1246
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 502
XspI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
ZrmI AGTACT 1 cut(s) 1006
Zsp2I ATGCAT 2 cut(s) 375, 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.