RchiOBHm_Chr4g0408211

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012605
Physical Location & Seq
Reverse (-)
30834650 .. 30839773
5124 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37938

Sequence Viewer

Length: 1827 bp
ATGTTGTCTACATCCAAACATGGCGCTCTCACTCTTTCTTCAATTCTCTTCAAGCATCTCACACCCCTTACAGCATTTTCACCATTCTCTTCAAAACCCAATTCAAATTTCACAACCCCAAATCAGATTTCCACAATCCCAAAACAACCCGCTGCAAGAACAAGAGTCCCACAGGCTTCAGACCCAGTAAGTGCTTCTGTTGTCAATTCAGTATGTTCTTTACTTTCTAGAGAAACCGCCCATGTTGATAGTTTGTTGAGTGGTTTCAAACACGAGTTGAGTTCAGAAGTGGTGCTTCAAGTTTTGATGAATTATAAGCAGCTGGGAAGGAGAAAGACATTGGACTTCTTTTCTTGGGCTGGGTTTCAAATGCGTTTTCAGTTTGATGACTGTGTGGTTGAGTACATGGCTGATTTCTTAGGTAGGAGGAAGCTATTTGATGACATCAAGTGTCTTTTATTGACAGTGTCCTTGCACAAGGGTCGGGTTTCGTGTCGAACAGTTTCGACTTGTATTAGGTTTCTGGGTAGGCAGGGGAGGATTAAAGAAGCACTTTGCGTGTTTGAAGAAATGGAATCTAAATTTGGGTGTAAACCTGATAATCTTGTGTACAATAATGTGCTTTATGTGCTTTGTAAGAAGGAGTCATCTGGGCATTTGATTGATTTTGCGCTTGCAGTTTTTCGGAGAATTGAGTCCCCTGATATGTATTCGTATAGTAATATGCTTGTTGGTTTGTGTAAATTTGGTAGATTTGAGACTGCTGTTGAAGTTTTTGGTGAAATGTGTGGGACTGGTTTGGTTCCTACTAGGTCTGCGGTGAATGTGCTTATTGGAGAGTTGTGTTTATTGAGTGCGAAAGAAGCAGCTGTTGCTAAAGTAAGAGTGAAACATGTTCGTAGGCCGTTTAAGATTTTGGTTCCAAATGTGCATGAAAAGAGCGGTGCTATCCAGCCTGCACTTGGAGTGTTTTGGGAAGTTTATAAACTAGGCTTATTACCTAGTACTTTTGTCATAATCCGACTTCTATCAGAGCTTTGTCAACTGGGAAAAATGGAAGAGGCTGTTCGGGTCTTGAAGGCTGTTGAGGGTAGGAAGCAGAGTTGCATTGAGGAGGGCTTCTGTATTGTGATGAAGGGATTGTGTGAACACTGCTGTGTTGAGGAGGCTAGTCATTTGTATGGGAGGATGCTCTCTCAGGGCATGAAGCCAAAGTTGAGTATTTATAATTCTATTATTTGCATGCTATGCAAACTAGGAAATTTGGATGGTGCTGAAAGTGTCTTTAAGATAATGAACAAGAAGAGATGTCTTCCGGATAGTTGTACTTATTCTGCATTGGTCCATGCGTATTGTGAAGCTGGAAAATGGAAAGCTGCTTATGACTTAATGATAGAAATGCTCGGTTTGGGCTGCTCTGCGCCATTTCACACTTATAGCTTAGTGGATAAACTTTTGAGAGAAAATGAACAAATGGATTTGTGTTTTAAATTAGAAAGGAAGTTGGAAACCCAGATCTTGGAGAAGCTATGCAAAGATGGTCGATTAGAGGATGCGTATGAGAAGCTAAAGTCAATGATTGAAAAGGGGTTTTACCCACCAGTTTATGCGAGAGATGCTTTTAAGAATGCATTTCGGAAGTATGGAAAATTAAAAATAGCTCAGGATTTGTTACAGACGATAGATGACATAGACAACAATGAGGAGAAACAACTTACCAATGAACCTATTGACAGAAGTATTTTCCACCGTCAACTAAAGAAGACAATGATGCAGACAAATGGAAGAGCGAGGAAAGATCAAACCAGCAATGAAGTTGTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

608

Amino Acids

68.68

Weight (kDa)

8.94

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 213 9.9e-07 PPR repeat family
PPR_1 PF12854 233 - 262 7.7e-06 PPR repeat
PPR_2 PF13041 234 - 282 2e-07 PPR repeat family
PPR_long PF17177 387 - 469 7.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 397 - 451 4.7e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 401 - 432 4.1e-06 PPR repeat
PPR_2 PF13041 406 - 452 5.5e-13 PPR repeat family
PPR PF01535 408 - 437 9.8e-06 PPR repeat
PPR_1 PF12854 436 - 467 1.8e-08 PPR repeat
PPR_2 PF13041 442 - 480 3.3e-06 PPR repeat family
PPR PF01535 443 - 472 6.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 315, 984, 1227
AccB7I CCANNNNNTGG 1 cut(s) 1519
AccBSI CCGCTC 1 cut(s) 942
AccI GTMKAC 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 1315
AciI CCGC 4 cut(s) 150, 237, 818, 942
AcsI RAATTY 4 cut(s) 106, 581, 743, 1261
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 4 cut(s) 404, 611, 1006, 1327
AfiI CCNNNNNNNGG 2 cut(s) 962, 1519
AflIII ACRYGT 1 cut(s) 892
AjuI GAANNNNNNNTTGG 6 cut(s) 92, 112, 124, 144, 567, 599
AleI CACNNNNGTG 1 cut(s) 1155
Alw26I GTCTC 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 1315
AoxI GGCC 1 cut(s) 902
ApeKI GCWGC 5 cut(s) 152, 319, 866, 1376, 1413
ApoI RAATTY 4 cut(s) 106, 581, 743, 1261
Asp700I GAANNNNTTC 1 cut(s) 502
AspLEI GCGC 3 cut(s) 26, 673, 1423
AspS9I GGNCC 1 cut(s) 1342
AsuHPI GGTGA 3 cut(s) 72, 791, 832
AvaII GGWCC 1 cut(s) 1342
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 1304, 1769
BbvI GCAGC 5 cut(s) 139, 331, 878, 1363, 1400
BccI CCATC 2 cut(s) 1262, 1532
BceAI ACGGC 1 cut(s) 889
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
BfoI RGCGCY 1 cut(s) 27
BglII AGATCT 1 cut(s) 1515
BisI GCNGC 5 cut(s) 153, 320, 867, 1377, 1414
BlsI GCNGC 5 cut(s) 154, 321, 868, 1378, 1415
BmcAI AGTACT 1 cut(s) 1006
Bme18I GGWCC 1 cut(s) 1342
BmgT120I GGNCC 1 cut(s) 1342
BmiI GGNNCC 2 cut(s) 804, 921
BmrI ACTGGG 2 cut(s) 179, 1055
BmsI GCATC 5 cut(s) 64, 1179, 1543, 1606, 1761
BmuI ACTGGG 2 cut(s) 179, 1055
BpiI GAAGAC 2 cut(s) 1304, 1769
Bpu10I CCTNAGC 1 cut(s) 1662
BsaWI WCCGGW 1 cut(s) 1315
Bsc4I CCNNNNNNNGG 2 cut(s) 962, 1519
Bse1I ACTGG 4 cut(s) 185, 799, 1050, 1601
Bse3DI GCAATG 1 cut(s) 1816
BseAI TCCGGA 1 cut(s) 1315
BseGI GGATG 4 cut(s) 11, 1194, 1273, 1558
BseLI CCNNNNNNNGG 2 cut(s) 962, 1519
BseMI GCAATG 1 cut(s) 1816
BseMII CTCAG 2 cut(s) 1211, 1676
BseNI ACTGG 4 cut(s) 185, 799, 1050, 1601
BseRI GAGGAG 3 cut(s) 1127, 1178, 1718
BseXI GCAGC 5 cut(s) 139, 331, 878, 1363, 1400
BseYI CCCAGC 2 cut(s) 322, 359
BsgI GTGCAG 1 cut(s) 942
BshFI GGCC 1 cut(s) 904
BsiSI CCGG 1 cut(s) 1316
BslFI GGGAC 3 cut(s) 152, 682, 805
BslI CCNNNNNNNGG 2 cut(s) 962, 1519
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 3 cut(s) 152, 682, 805
BsmI GAATGC 1 cut(s) 1633
BsnI GGCC 1 cut(s) 904
Bsp13I TCCGGA 1 cut(s) 1315
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 2 cut(s) 1515, 1798
BspACI CCGC 4 cut(s) 150, 237, 818, 942
BspANI GGCC 1 cut(s) 904
BspCNI CTCAG 2 cut(s) 1210, 1675
BspEI TCCGGA 1 cut(s) 1315
BspLI GGNNCC 2 cut(s) 804, 921
BspQI GCTCTTC 1 cut(s) 1780
BsrBI CCGCTC 1 cut(s) 942
BsrDI GCAATG 1 cut(s) 1816
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 4 cut(s) 185, 799, 1050, 1601
BssMI GATC 2 cut(s) 1515, 1798
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 392, 466, 502, 1751
Bst6I CTCTTC 5 cut(s) 53, 94, 1053, 1298, 1780
BstAPI GCANNNNNTGC 2 cut(s) 872, 1248
BstAUI TGTACA 1 cut(s) 609
BstC8I GCNNGC 3 cut(s) 675, 957, 1244
BstDEI CTNAG 4 cut(s) 418, 1197, 1441, 1662
BstF5I GGATG 4 cut(s) 11, 1194, 1273, 1558
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 3 cut(s) 26, 673, 1423
BstKTI GATC 2 cut(s) 1518, 1801
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 2 cut(s) 1515, 1798
BstMWI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
BstNSI RCATGY 2 cut(s) 896, 1246
BstV1I GCAGC 5 cut(s) 139, 331, 878, 1363, 1400
BstV2I GAAGAC 2 cut(s) 1304, 1769
BstX2I RGATCY 1 cut(s) 1515
BstYI RGATCY 1 cut(s) 1515
BsuRI GGCC 1 cut(s) 904
BtsCI GGATG 4 cut(s) 11, 1194, 1273, 1558
BtsI GCAGTG 1 cut(s) 1150
BtsIMutI CAGTG 2 cut(s) 471, 1150
Cac8I GCNNGC 3 cut(s) 675, 957, 1244
CfoI GCGC 3 cut(s) 26, 673, 1423
Cfr13I GGNCC 1 cut(s) 1342
Csp6I GTAC 4 cut(s) 403, 610, 1005, 1326
CviAII CATG 8 cut(s) 20, 242, 406, 893, 932, 1204, 1243, 1346
CviQI GTAC 4 cut(s) 403, 610, 1005, 1326
DdeI CTNAG 4 cut(s) 418, 1197, 1441, 1662
DpnI GATC 2 cut(s) 1517, 1800
DpnII GATC 2 cut(s) 1515, 1798
DraI TTTAAA 1 cut(s) 1489
Eam1104I CTCTTC 5 cut(s) 53, 94, 1053, 1298, 1780
EarI CTCTTC 5 cut(s) 53, 94, 1053, 1298, 1780
Eco47I GGWCC 1 cut(s) 1342
Eco57I CTGAAG 1 cut(s) 162
EcoT22I ATGCAT 1 cut(s) 1633
FaeI CATG 8 cut(s) 23, 245, 409, 896, 935, 1207, 1246, 1349
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 537, 569
FaqI GGGAC 3 cut(s) 152, 682, 805
FatI CATG 8 cut(s) 19, 241, 405, 892, 931, 1203, 1242, 1345
FauI CCCGC 1 cut(s) 157
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 5 cut(s) 153, 320, 867, 1377, 1414
FokI GGATG 3 cut(s) 1201, 1280, 1565
Fsp4HI GCNGC 5 cut(s) 153, 320, 867, 1377, 1414
FspBI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
GlaI GCGC 3 cut(s) 25, 672, 1422
GluI GCNGC 5 cut(s) 153, 320, 867, 1377, 1414
GsaI CCCAGC 2 cut(s) 326, 363
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 1 cut(s) 904
HapII CCGG 1 cut(s) 1316
HhaI GCGC 3 cut(s) 26, 673, 1423
Hin1II CATG 8 cut(s) 23, 245, 409, 896, 935, 1207, 1246, 1349
Hin6I GCGC 3 cut(s) 24, 671, 1421
HinP1I GCGC 3 cut(s) 24, 671, 1421
HincII GTYRAC 2 cut(s) 1043, 1754
HindII GTYRAC 2 cut(s) 1043, 1754
HinfI GANTC 4 cut(s) 165, 575, 644, 695
HpaII CCGG 1 cut(s) 1316
HphI GGTGA 3 cut(s) 72, 791, 832
Hpy166II GTNNAC 6 cut(s) 9, 593, 610, 1043, 1148, 1754
Hpy188I TCNGA 7 cut(s) 126, 181, 286, 687, 1022, 1033, 1638
Hpy188III TCNNGA 4 cut(s) 228, 1075, 1316, 1664
Hpy8I GTNNAC 6 cut(s) 9, 593, 610, 1043, 1148, 1754
HpyAV CCTTC 4 cut(s) 321, 634, 1072, 1129
HpyCH4III ACNGT 4 cut(s) 392, 466, 502, 1751
HpyF10VI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
HpyF3I CTNAG 4 cut(s) 418, 1197, 1441, 1662
Hsp92II CATG 8 cut(s) 23, 245, 409, 896, 935, 1207, 1246, 1349
HspAI GCGC 3 cut(s) 24, 671, 1421
Kpn2I TCCGGA 1 cut(s) 1315
Kzo9I GATC 2 cut(s) 1515, 1798
LguI GCTCTTC 1 cut(s) 1780
Lsp1109I GCAGC 5 cut(s) 139, 331, 878, 1363, 1400
LweI GCATC 5 cut(s) 64, 1179, 1543, 1606, 1761
MaeI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
MaeIII GTNAC 1 cut(s) 1671
MalI GATC 2 cut(s) 1517, 1800
MbiI CCGCTC 1 cut(s) 942
MboI GATC 2 cut(s) 1515, 1798
MboII GAAGA 9 cut(s) 30, 40, 81, 578, 1070, 1304, 1315, 1774, 1797
MflI RGATCY 1 cut(s) 1515
MlyI GAGTC 3 cut(s) 174, 653, 704
MmeI TCCRAC 2 cut(s) 1045, 1485
Mph1103I ATGCAT 1 cut(s) 1633
MroI TCCGGA 1 cut(s) 1315
MroXI GAANNNNTTC 1 cut(s) 502
MseI TTAA 7 cut(s) 543, 909, 1287, 1388, 1488, 1623, 1652
MslI CAYNNNNRTG 2 cut(s) 1155, 1179
MspA1I CMGCKG 3 cut(s) 152, 322, 869
MspI CCGG 1 cut(s) 1316
Mva1269I GAATGC 1 cut(s) 1633
MwoI GCNNNNNNNGC 5 cut(s) 628, 863, 872, 1248, 1616
NdeII GATC 2 cut(s) 1515, 1798
NlaIII CATG 8 cut(s) 23, 245, 409, 896, 935, 1207, 1246, 1349
NlaIV GGNNCC 2 cut(s) 804, 921
NsiI ATGCAT 1 cut(s) 1633
NspI RCATGY 2 cut(s) 896, 1246
OliI CACNNNNGTG 1 cut(s) 1155
PaeI GCATGC 1 cut(s) 1246
PciI ACATGT 1 cut(s) 892
PciSI GCTCTTC 1 cut(s) 1780
PctI GAATGC 1 cut(s) 1633
PdmI GAANNNNTTC 1 cut(s) 502
PfeI GAWTC 1 cut(s) 575
PflFI GACNNNGTC 1 cut(s) 466
PflMI CCANNNNNTGG 1 cut(s) 1519
PkrI GCNGC 5 cut(s) 154, 321, 868, 1378, 1415
PleI GAGTC 3 cut(s) 173, 652, 703
PpsI GAGTC 3 cut(s) 173, 652, 703
PscI ACATGT 1 cut(s) 892
PsiI TTATAA 3 cut(s) 315, 984, 1227
PspFI CCCAGC 2 cut(s) 322, 359
PspN4I GGNNCC 2 cut(s) 804, 921
PspPI GGNCC 1 cut(s) 1342
PsuI RGATCY 1 cut(s) 1515
PsyI GACNNNGTC 1 cut(s) 466
PvuII CAGCTG 2 cut(s) 322, 869
RsaI GTAC 4 cut(s) 404, 611, 1006, 1327
RsaNI GTAC 4 cut(s) 403, 610, 1005, 1326
RseI CAYNNNNRTG 2 cut(s) 1155, 1179
SapI GCTCTTC 1 cut(s) 1780
SaqAI TTAA 7 cut(s) 543, 909, 1287, 1388, 1488, 1623, 1652
SatI GCNGC 5 cut(s) 153, 320, 867, 1377, 1414
Sau3AI GATC 2 cut(s) 1515, 1798
Sau96I GGNCC 1 cut(s) 1342
ScaI AGTACT 1 cut(s) 1006
SchI GAGTC 3 cut(s) 174, 653, 704
SfaNI GCATC 5 cut(s) 64, 1179, 1543, 1606, 1761
SinI GGWCC 1 cut(s) 1342
SmiMI CAYNNNNRTG 2 cut(s) 1155, 1179
SphI GCATGC 1 cut(s) 1246
SsiI CCGC 4 cut(s) 150, 237, 818, 942
SspMI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
TaaI ACNGT 4 cut(s) 392, 466, 502, 1751
TaqI TCGA 3 cut(s) 496, 506, 1543
TatI WGTACW 4 cut(s) 402, 609, 1004, 1325
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 7 cut(s) 543, 909, 1287, 1388, 1488, 1623, 1652
Tru9I TTAA 7 cut(s) 543, 909, 1287, 1388, 1488, 1623, 1652
TscAI CASTG 2 cut(s) 471, 1157
TseI GCWGC 5 cut(s) 152, 319, 866, 1376, 1413
TspDTI ATGAA 8 cut(s) 323, 948, 1148, 1220, 1310, 1482, 1737, 1827
TspRI CASTG 2 cut(s) 471, 1157
Tth111I GACNNNGTC 1 cut(s) 466
Van91I CCANNNNNTGG 1 cut(s) 1519
VpaK11BI GGWCC 1 cut(s) 1342
XapI RAATTY 4 cut(s) 106, 581, 743, 1261
XbaI TCTAGA 1 cut(s) 227
XceI RCATGY 2 cut(s) 896, 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 959
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 502
XspI CTAG 6 cut(s) 228, 810, 989, 1002, 1170, 1256
ZrmI AGTACT 1 cut(s) 1006
Zsp2I ATGCAT 1 cut(s) 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.