FvH4_4g14440

Adenylate isopentenyltransferase 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011736
Physical Location & Seq
Reverse (-)
18112967 .. 18115648
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14440.t1

Sequence Viewer

Length: 1062 bp
ATGCAAACTCTTTTCTCCATGTCTGCCTGCCAACAAGTAGTTCAAATACAGCCGATGATAAGATTCCAAGGGCCGCCAGCCGGACTATCCATCGATCGGTTTTTCAGTCGGAATAAGGATAAGGTTGTGATAGTAATCGGAGCTACCGGAACCGGTAAGTCCAGGCTTGCCATAGACCTTGCCTTAAAATATTCGGCCGAGATCATAAACTCAGATAAAATGCAAGTCTATAAAGGCTTGGACACCCTCACCAACAAGGTCACTGAGGAGGAGTGCAAAGGTGTACCACATCATTTGTTGGGCACAGTGGATCCTGACTCGAACTTCACTGCTACTGATTTCAAGCATCATGCTTCTTTGGCCATTGAATCGATATTGGAGCGTGACCGGTTACCGATCATCGCCGGTGGGTCAAACTCTTACATAGAGGCACTGGTGGACGAGCACGCCGAGTTCCGGAGGAGGTACGAGTGTTGCTTTCTGTGGGTGGACGTGGCGCCGCGTGTGCTCAACTCGTTTGTGTCGGAGCGAGTTGATCGGATGGTGGAGGTGGGTGGTCTGGTGGACGAGGTGAGGAGAATGTTCGACCCTACTACTGCCGACGAGGAGGACTACAGCACAGGGATCAGAAAAGCCATCGGTGTTCCGGAGATGCATCATTACTTGTGTGGCGAAGCATGTGGCAACGATAAAGAAACCCAAGAGGCGCTTCTGTTGGAAGCCATTTCCAACATCAAGAAGAATACTTGCACGTTGGCTCATCGACAGTTGCAAAAGATTCACCGGCTGCGGAGCAAGTGGAACTGGAGCATGCACTGCCTCGACGCCACCGAAGCTTTCTTGCACCGCCGCCGCGGCCGGGAGGGAGAACTGGTGTGGGACAAGCAAGTCTCTAGACCCGCTACAGGAATTGTCAGTAAGTTCCTCGTAGACGACCCAATGTCAACTACAATTCTTCCTCATGACACCGCCTTTGTGGCTGCAACGGCCACGCCCGCCACCACCGCCAGCGTTAACTCTCTAACGGTGTCCATGTCGGCCGTGGCCGCTGCAACACGGTAG

Protein Analysis

354

Amino Acids

39.28

Weight (kDa)

6.32

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPT PF01745 41 - 151 8e-10 Isopentenyl transferase
IPPT PF01715 75 - 151 6.9e-23 IPP transferase
IPPT PF01715 152 - 258 9.9e-13 IPP transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011736)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23630 AT5G19040 AT5G19040 AT5G19040
fragaria_vesca FvH4_4g14440
malus_domestica MD13G1182800.v1.1 MD16G1183400.v1.1
prunus_persica Prupe.1G150800_v2.0.a1
pyrus_communis pycom13g15770 pycom16g15410
rosa_chinensis RchiOBHm_Chr4g0418401
rosa_laevigata RLG00000007870
rosa_multiflora Rmu_sc0001266.1_g000013
rosa_roxburghii Rroxscaffold_5G00361580
rosa_rugosa Rorug04G0152700
rosa_samantha Rh4BG216100 Rh4CG226100 Rh4DG212800
rosa_wichuraiana Rw4G018210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 887
AccB1I GGYRCC 1 cut(s) 496
AccI GTMKAC 1 cut(s) 930
AccII CGCG 2 cut(s) 502, 855
AccIII TCCGGA 2 cut(s) 456, 646
AclWI GGATC 3 cut(s) 305, 318, 632
AcoI YGGCCR 6 cut(s) 195, 360, 856, 987, 1038, 1044
AcyI GRCGYC 2 cut(s) 497, 825
AfaI GTAC 2 cut(s) 285, 467
AfiI CCNNNNNNNGG 5 cut(s) 80, 96, 456, 859, 905
AgeI ACCGGT 2 cut(s) 152, 387
AgsI TTSAA 3 cut(s) 44, 343, 368
AhdI GACNNNNNGTC 1 cut(s) 940
AjiI CACGTC 1 cut(s) 493
AjnI CCWGG 1 cut(s) 161
AjuI GAANNNNNNNTTGG 4 cut(s) 24, 56, 693, 725
AluBI AGCT 2 cut(s) 143, 836
AluI AGCT 2 cut(s) 143, 836
Alw21I GWGCWC 2 cut(s) 447, 510
Alw26I GTCTC 1 cut(s) 895
AlwI GGATC 3 cut(s) 305, 318, 632
Aor13HI TCCGGA 2 cut(s) 456, 646
AoxI GGCC 7 cut(s) 71, 195, 360, 856, 987, 1038, 1044
ApeKI GCWGC 3 cut(s) 787, 980, 1049
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
AsiGI ACCGGT 2 cut(s) 152, 387
AspLEI GCGC 2 cut(s) 499, 709
AspS9I GGNCC 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 860
AsuHPI GGTGA 3 cut(s) 241, 583, 773
BaeGI GKGCMC 1 cut(s) 305
BaeI ACNNNNGTAYC 2 cut(s) 457, 490
BalI TGGCCA 1 cut(s) 362
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 496
Bbv12I GWGCWC 2 cut(s) 447, 510
BbvI GCAGC 3 cut(s) 774, 967, 1036
BccI CCATC 3 cut(s) 98, 535, 644
BceAI ACGGC 2 cut(s) 1002, 1025
BciT130I CCWGG 1 cut(s) 163
BcnI CCSGG 1 cut(s) 860
BcoDI GTCTC 1 cut(s) 895
BfaI CTAG 1 cut(s) 894
BfmI CTRYAG 2 cut(s) 613, 903
BfoI RGCGCY 2 cut(s) 500, 710
BglI GCCNNNNNGGC 2 cut(s) 855, 977
BisI GCNGC 9 cut(s) 74, 500, 788, 850, 853, 856, 981, 1047, 1050
BlsI GCNGC 9 cut(s) 75, 501, 789, 851, 854, 857, 982, 1048, 1051
Bme1390I CCNGG 2 cut(s) 163, 860
BmeRI GACNNNNNGTC 1 cut(s) 940
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 3 cut(s) 151, 312, 498
BmrFI CCNGG 2 cut(s) 163, 860
BmsI GCATC 3 cut(s) 355, 642, 664
BpmI CTGGAG 1 cut(s) 826
BpuMI CCSGG 1 cut(s) 860
Bsa29I ATCGAT 2 cut(s) 93, 371
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 2 cut(s) 497, 825
BsaJI CCNNGG 3 cut(s) 67, 853, 1041
BsaWI WCCGGW 5 cut(s) 146, 152, 387, 456, 646
BsaXI ACNNNNNCTCC 2 cut(s) 858, 888
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 96, 456, 859, 905
Bse118I RCCGGY 4 cut(s) 152, 387, 404, 783
Bse1I ACTGG 3 cut(s) 438, 809, 876
Bse8I GATNNNNATC 1 cut(s) 134
BseAI TCCGGA 2 cut(s) 456, 646
BseBI CCWGG 1 cut(s) 163
BseCI ATCGAT 2 cut(s) 93, 371
BseDI CCNNGG 3 cut(s) 67, 853, 1041
BseGI GGATG 1 cut(s) 546
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 5 cut(s) 80, 96, 456, 859, 905
BseMII CTCAG 2 cut(s) 225, 255
BseNI ACTGG 3 cut(s) 438, 809, 876
BseRI GAGGAG 5 cut(s) 281, 284, 475, 589, 620
BseSI GKGCMC 1 cut(s) 305
BseX3I CGGCCG 3 cut(s) 195, 856, 1038
BseXI GCAGC 3 cut(s) 774, 967, 1036
Bsh1236I CGCG 2 cut(s) 502, 855
Bsh1285I CGRYCG 4 cut(s) 97, 198, 859, 1041
BshFI GGCC 7 cut(s) 73, 197, 362, 858, 989, 1040, 1046
BshNI GGYRCC 1 cut(s) 496
BshTI ACCGGT 2 cut(s) 152, 387
BshVI ATCGAT 2 cut(s) 93, 371
BsiEI CGRYCG 4 cut(s) 97, 198, 859, 1041
BsiHKAI GWGCWC 2 cut(s) 447, 510
BsiSI CCGG 9 cut(s) 81, 147, 153, 388, 405, 457, 647, 784, 859
BslFI GGGAC 1 cut(s) 893
BslI CCNNNNNNNGG 5 cut(s) 80, 96, 456, 859, 905
BsmAI GTCTC 1 cut(s) 895
BsmFI GGGAC 1 cut(s) 893
BsnI GGCC 7 cut(s) 73, 197, 362, 858, 989, 1040, 1046
Bsp1286I GDGCHC 3 cut(s) 305, 447, 510
Bsp13I TCCGGA 2 cut(s) 456, 646
Bsp143I GATC 6 cut(s) 94, 201, 310, 396, 535, 624
BspANI GGCC 7 cut(s) 73, 197, 362, 858, 989, 1040, 1046
BspCNI CTCAG 2 cut(s) 224, 256
BspDI ATCGAT 2 cut(s) 93, 371
BspEI TCCGGA 2 cut(s) 456, 646
BspFNI CGCG 2 cut(s) 502, 855
BspHI TCATGA 1 cut(s) 961
BspLI GGNNCC 3 cut(s) 151, 312, 498
BspPI GGATC 3 cut(s) 305, 318, 632
BspT107I GGYRCC 1 cut(s) 496
BsrFI RCCGGY 4 cut(s) 152, 387, 404, 783
BsrI ACTGG 3 cut(s) 438, 809, 876
BssAI RCCGGY 4 cut(s) 152, 387, 404, 783
BssECI CCNNGG 3 cut(s) 67, 853, 1041
BssMI GATC 6 cut(s) 94, 201, 310, 396, 535, 624
BssNI GRCGYC 2 cut(s) 497, 825
BssT1I CCWWGG 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 4 cut(s) 307, 768, 1027, 1059
BstACI GRCGYC 2 cut(s) 497, 825
BstAPI GCANNNNNTGC 1 cut(s) 816
BstC8I GCNNGC 7 cut(s) 28, 78, 168, 447, 812, 996, 1009
BstDEI CTNAG 2 cut(s) 211, 264
BstDSI CCRYGG 2 cut(s) 853, 1041
BstEII GGTNACC 1 cut(s) 390
BstF5I GGATG 1 cut(s) 546
BstFNI CGCG 2 cut(s) 502, 855
BstH2I RGCGCY 2 cut(s) 500, 710
BstHHI GCGC 2 cut(s) 499, 709
BstKTI GATC 6 cut(s) 97, 204, 313, 399, 538, 627
BstMAI GTCTC 1 cut(s) 895
BstMBI GATC 6 cut(s) 94, 201, 310, 396, 535, 624
BstMCI CGRYCG 4 cut(s) 97, 198, 859, 1041
BstNI CCWGG 1 cut(s) 163
BstNSI RCATGY 2 cut(s) 681, 814
BstPI GGTNACC 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 161, 858
BstSFI CTRYAG 2 cut(s) 613, 903
BstSLI GKGCMC 1 cut(s) 305
BstUI CGCG 2 cut(s) 502, 855
BstV1I GCAGC 3 cut(s) 774, 967, 1036
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZI CGGCCG 3 cut(s) 195, 856, 1038
Bsu15I ATCGAT 2 cut(s) 93, 371
BsuRI GGCC 7 cut(s) 73, 197, 362, 858, 989, 1040, 1046
BsuTUI ATCGAT 2 cut(s) 93, 371
BtgI CCRYGG 2 cut(s) 853, 1041
BtgZI GCGATG 1 cut(s) 385
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 1 cut(s) 546
BtsI GCAGTG 2 cut(s) 327, 814
BtsIMutI CAGTG 5 cut(s) 261, 312, 327, 431, 814
Cac8I GCNNGC 7 cut(s) 28, 78, 168, 447, 812, 996, 1009
CciI TCATGA 1 cut(s) 961
CfoI GCGC 2 cut(s) 499, 709
Cfr10I RCCGGY 4 cut(s) 152, 387, 404, 783
Cfr13I GGNCC 1 cut(s) 71
Cfr42I CCGCGG 1 cut(s) 856
ClaI ATCGAT 2 cut(s) 93, 371
CseI GACGC 1 cut(s) 833
Csp6I GTAC 2 cut(s) 284, 466
CspAI ACCGGT 2 cut(s) 152, 387
CspCI CAANNNNNGTGG 2 cut(s) 276, 311
CviAII CATG 6 cut(s) 19, 350, 678, 811, 962, 1033
CviQI GTAC 2 cut(s) 284, 466
DdeI CTNAG 2 cut(s) 211, 264
DinI GGCGCC 1 cut(s) 498
DpnI GATC 6 cut(s) 96, 203, 312, 398, 537, 626
DpnII GATC 6 cut(s) 94, 201, 310, 396, 535, 624
DrdI GACNNNNNNGTC 1 cut(s) 887
DriI GACNNNNNGTC 1 cut(s) 940
DseDI GACNNNNNNGTC 1 cut(s) 887
EaeI YGGCCR 6 cut(s) 195, 360, 856, 987, 1038, 1044
EagI CGGCCG 3 cut(s) 195, 856, 1038
Eam1105I GACNNNNNGTC 1 cut(s) 940
EclXI CGGCCG 3 cut(s) 195, 856, 1038
Eco130I CCWWGG 1 cut(s) 67
Eco52I CGGCCG 3 cut(s) 195, 856, 1038
Eco91I GGTNACC 1 cut(s) 390
EcoO65I GGTNACC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 657
EgeI GGCGCC 1 cut(s) 498
EheI GGCGCC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 6 cut(s) 22, 353, 681, 814, 965, 1036
FalI AAGNNNNNCTT 2 cut(s) 693, 725
FaqI GGGAC 1 cut(s) 893
FatI CATG 6 cut(s) 18, 349, 677, 810, 961, 1032
FauI CCCGC 2 cut(s) 907, 1003
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 9 cut(s) 74, 500, 788, 850, 853, 856, 981, 1047, 1050
FokI GGATG 1 cut(s) 553
Fsp4HI GCNGC 9 cut(s) 74, 500, 788, 850, 853, 856, 981, 1047, 1050
FspBI CTAG 1 cut(s) 894
GlaI GCGC 2 cut(s) 498, 708
GluI GCNGC 9 cut(s) 74, 500, 788, 850, 853, 856, 981, 1047, 1050
GsuI CTGGAG 1 cut(s) 826
HaeII RGCGCY 2 cut(s) 500, 710
HaeIII GGCC 7 cut(s) 73, 197, 362, 858, 989, 1040, 1046
HapII CCGG 9 cut(s) 81, 147, 153, 388, 405, 457, 647, 784, 859
HgaI GACGC 1 cut(s) 833
HhaI GCGC 2 cut(s) 499, 709
Hin1I GRCGYC 2 cut(s) 497, 825
Hin1II CATG 6 cut(s) 22, 353, 681, 814, 965, 1036
Hin6I GCGC 2 cut(s) 497, 707
HinP1I GCGC 2 cut(s) 497, 707
HincII GTYRAC 2 cut(s) 945, 1015
HindII GTYRAC 2 cut(s) 945, 1015
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 4 cut(s) 63, 317, 368, 778
HpaI GTTAAC 1 cut(s) 1015
HpaII CCGG 9 cut(s) 81, 147, 153, 388, 405, 457, 647, 784, 859
HphI GGTGA 3 cut(s) 241, 583, 773
Hpy166II GTNNAC 7 cut(s) 284, 439, 490, 565, 931, 945, 1015
Hpy188I TCNGA 6 cut(s) 111, 140, 214, 526, 540, 629
Hpy188III TCNNGA 6 cut(s) 314, 457, 647, 736, 894, 962
Hpy8I GTNNAC 7 cut(s) 284, 439, 490, 565, 931, 945, 1015
Hpy99I CGWCG 2 cut(s) 605, 827
HpyCH4III ACNGT 4 cut(s) 307, 768, 1027, 1059
HpyCH4IV ACGT 2 cut(s) 492, 752
HpyF3I CTNAG 2 cut(s) 211, 264
HpySE526I ACGT 2 cut(s) 492, 752
Hsp92I GRCGYC 2 cut(s) 497, 825
Hsp92II CATG 6 cut(s) 22, 353, 681, 814, 965, 1036
HspAI GCGC 2 cut(s) 497, 707
KasI GGCGCC 1 cut(s) 496
Kpn2I TCCGGA 2 cut(s) 456, 646
KspAI GTTAAC 1 cut(s) 1015
KspI CCGCGG 1 cut(s) 856
Kzo9I GATC 6 cut(s) 94, 201, 310, 396, 535, 624
LmnI GCTCC 5 cut(s) 140, 379, 526, 792, 807
Lsp1109I GCAGC 3 cut(s) 774, 967, 1036
LweI GCATC 3 cut(s) 355, 642, 664
MaeI CTAG 1 cut(s) 894
MaeII ACGT 2 cut(s) 492, 752
MaeIII GTNAC 3 cut(s) 259, 383, 390
MalI GATC 6 cut(s) 96, 203, 312, 398, 537, 626
MboI GATC 6 cut(s) 94, 201, 310, 396, 535, 624
MboII GAAGA 2 cut(s) 751, 947
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 3 cut(s) 305, 447, 510
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 2 cut(s) 909, 951
MluNI TGGCCA 1 cut(s) 362
Mly113I GGCGCC 1 cut(s) 497
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 4 cut(s) 89, 504, 696, 753
Mox20I TGGCCA 1 cut(s) 362
Mph1103I ATGCAT 1 cut(s) 657
MroI TCCGGA 2 cut(s) 456, 646
MscI TGGCCA 1 cut(s) 362
MseI TTAA 2 cut(s) 185, 1014
Msp20I TGGCCA 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 855, 1049
MspI CCGG 9 cut(s) 81, 147, 153, 388, 405, 457, 647, 784, 859
MspR9I CCNGG 2 cut(s) 163, 860
MvaI CCWGG 1 cut(s) 163
MvnI CGCG 2 cut(s) 502, 855
NarI GGCGCC 1 cut(s) 497
NciI CCSGG 1 cut(s) 860
NdeII GATC 6 cut(s) 94, 201, 310, 396, 535, 624
NlaIII CATG 6 cut(s) 22, 353, 681, 814, 965, 1036
NlaIV GGNNCC 3 cut(s) 151, 312, 498
NmeAIII GCCGAG 2 cut(s) 223, 475
NmuCI GTSAC 2 cut(s) 259, 383
NsiI ATGCAT 1 cut(s) 657
NspI RCATGY 2 cut(s) 681, 814
PaeI GCATGC 1 cut(s) 814
PagI TCATGA 1 cut(s) 961
PcsI WCGNNNNNNNCGW 2 cut(s) 447, 828
PfeI GAWTC 3 cut(s) 63, 368, 778
PinAI ACCGGT 2 cut(s) 152, 387
PkrI GCNGC 9 cut(s) 75, 501, 789, 851, 854, 857, 982, 1048, 1051
Ple19I CGATCG 1 cut(s) 97
PleI GAGTC 1 cut(s) 311
PluTI GGCGCC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 161
PspEI GGTNACC 1 cut(s) 390
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 3 cut(s) 151, 312, 498
PspPI GGNCC 1 cut(s) 71
PsuI RGATCY 1 cut(s) 310
PvuI CGATCG 1 cut(s) 97
RsaI GTAC 2 cut(s) 285, 467
RsaNI GTAC 2 cut(s) 284, 466
SacII CCGCGG 1 cut(s) 856
SaqAI TTAA 2 cut(s) 185, 1014
SatI GCNGC 9 cut(s) 74, 500, 788, 850, 853, 856, 981, 1047, 1050
Sau3AI GATC 6 cut(s) 94, 201, 310, 396, 535, 624
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 163, 860
SduI GDGCHC 3 cut(s) 305, 447, 510
SfaNI GCATC 3 cut(s) 355, 642, 664
SfcI CTRYAG 2 cut(s) 613, 903
SfoI GGCGCC 1 cut(s) 498
Sfr303I CCGCGG 1 cut(s) 856
SgrAI CRCCGGYG 1 cut(s) 404
SgrBI CCGCGG 1 cut(s) 856
SphI GCATGC 1 cut(s) 814
Sse9I AATT 2 cut(s) 909, 951
SspDI GGCGCC 1 cut(s) 496
SspI AATATT 1 cut(s) 191
SspMI CTAG 1 cut(s) 894
StyD4I CCNGG 2 cut(s) 161, 858
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 4 cut(s) 307, 768, 1027, 1059
TaiI ACGT 2 cut(s) 495, 755
TaqI TCGA 6 cut(s) 93, 320, 371, 585, 763, 822
TasI AATT 2 cut(s) 909, 951
TauI GCSGC 6 cut(s) 76, 502, 852, 855, 858, 1049
TfiI GAWTC 3 cut(s) 63, 368, 778
Tru1I TTAA 2 cut(s) 185, 1014
Tru9I TTAA 2 cut(s) 185, 1014
TscAI CASTG 5 cut(s) 268, 312, 334, 438, 821
TseFI GTSAC 2 cut(s) 259, 383
TseI GCWGC 3 cut(s) 787, 980, 1049
Tsp45I GTSAC 2 cut(s) 259, 383
TspRI CASTG 5 cut(s) 268, 312, 334, 438, 821
XbaI TCTAGA 1 cut(s) 893
XceI RCATGY 2 cut(s) 681, 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 1 cut(s) 894
Zsp2I ATGCAT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.