pycom13g15770

Adenylate isopentenyltransferase 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011736
Physical Location & Seq
Reverse (-)
11533775 .. 11534575
801 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g15770.1

Sequence Viewer

Length: 801 bp
ATGCAAGTCTACAAAGGCTTGGACATCGTCACCAACAAAGTCACTGAGGACGAGTGTAAAGGCATACCTCACCATTTGATAGGCAAAGTCGATCCCGACTCAGATTTTACTGCCAATGATTTCAAGCACCATGCGTCGTTGGCCATCGAATCGATCTTGGAGCGTGACAGATTGCCAATCATTGCCGGGGGTTCAAATTCGTATATTGAAGCCTTGGTGGATGAACACTCGGAGTTTCGAATGAAGTACGAGTGTTGCTTTCTTTGGGTTGATGTGGCCATCCCCGTGCTCCACTCTTTCGTGTCAGAGAGAGTGAACCGGATGGTGAAGATGGGTTTGGTGGAAGAGGTGAGAAGAATGTTTGACTCTACAACTGAGAAAGAATATACGCGCGGAATCAGGCGAGCCATAGGCGTGCCTGAAATGGATGAGTATTTACAAGGCGAAGCAAGTGGTAATAACGATGATGAAACTCAAGAGAGGCTTCTTCAAGCAGCAATTTCTAAAATCAAAGAAAATACTTGCATTTTGGCGTATCGCCAATTGCAAAAGATTCGCCGGCTGCAAGCTCGGCGGGGTTGGAACATGCACCGCCTTGATGCCACGGAGGCATTCTTAAAGCACCACGCGGAAACGAATCAAGCGTGGGAAAAACTCGTGGCAAGACCTGCCACGCGGATAGTGCGTCAGTTCCTTTTGGATCCAATTCCCTCCCTTCTTCCGACAGAGAACATCACCGCCACTACCACGGCTGCGGTTCTTTCCACGTCTATGCCGACAGTGGCCACTGCAACACGATAG

Protein Analysis

267

Amino Acids

30.26

Weight (kDa)

6.16

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPPT PF01715 2 - 79 3.6e-24 IPP transferase
IPPT PF01715 80 - 186 1.9e-14 IPP transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011736)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23630 AT5G19040 AT5G19040 AT5G19040
fragaria_vesca FvH4_4g14440
malus_domestica MD13G1182800.v1.1 MD16G1183400.v1.1
prunus_persica Prupe.1G150800_v2.0.a1
pyrus_communis pycom13g15770 pycom16g15410
rosa_chinensis RchiOBHm_Chr4g0418401
rosa_laevigata RLG00000007870
rosa_multiflora Rmu_sc0001266.1_g000013
rosa_roxburghii Rroxscaffold_5G00361580
rosa_rugosa Rorug04G0152700
rosa_samantha Rh4BG216100 Rh4CG226100 Rh4DG212800
rosa_wichuraiana Rw4G018210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AccI GTMKAC 1 cut(s) 9
AccII CGCG 4 cut(s) 391, 393, 629, 676
AciI CCGC 7 cut(s) 393, 574, 592, 629, 676, 738, 755
AclWI GGATC 3 cut(s) 86, 695, 708
AcoI YGGCCR 3 cut(s) 141, 276, 783
AcsI RAATTY 1 cut(s) 196
AfaI GTAC 1 cut(s) 248
AgsI TTSAA 4 cut(s) 124, 195, 209, 491
AjiI CACGTC 1 cut(s) 768
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 1 cut(s) 569
AluI AGCT 1 cut(s) 569
Alw21I GWGCWC 1 cut(s) 291
AlwI GGATC 3 cut(s) 86, 695, 708
AoxI GGCC 3 cut(s) 141, 276, 783
ApeKI GCWGC 3 cut(s) 494, 562, 752
ApoI RAATTY 1 cut(s) 196
AspLEI GCGC 1 cut(s) 393
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 5 cut(s) 22, 62, 337, 361, 727
AsuII TTCGAA 1 cut(s) 238
BalI TGGCCA 3 cut(s) 143, 278, 785
BamHI GGATCC 1 cut(s) 700
BauI CACGAG 1 cut(s) 656
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 3 cut(s) 506, 549, 739
BccI CCATC 4 cut(s) 152, 287, 316, 325
BceAI ACGGC 1 cut(s) 765
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BcnI CCSGG 1 cut(s) 187
BfuAI ACCTGC 1 cut(s) 676
BglI GCCNNNNNGGC 1 cut(s) 608
BisI GCNGC 3 cut(s) 495, 563, 753
BlsI GCNGC 3 cut(s) 496, 564, 754
Bme1390I CCNGG 1 cut(s) 187
BmgBI CACGTC 1 cut(s) 768
BmiI GGNNCC 1 cut(s) 702
BmrFI CCNGG 1 cut(s) 187
BmsI GCATC 1 cut(s) 589
Bpu14I TTCGAA 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 187
Bsa29I ATCGAT 1 cut(s) 152
BsaJI CCNNGG 4 cut(s) 186, 213, 603, 747
BsaWI WCCGGW 1 cut(s) 318
Bse118I RCCGGY 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 180
BseCI ATCGAT 1 cut(s) 152
BseDI CCNNGG 4 cut(s) 186, 213, 603, 747
BseGI GGATG 4 cut(s) 226, 279, 327, 433
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 3 cut(s) 36, 114, 366
BseXI GCAGC 3 cut(s) 506, 549, 739
Bsh1236I CGCG 4 cut(s) 391, 393, 629, 676
BshFI GGCC 3 cut(s) 143, 278, 785
BshVI ATCGAT 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 3 cut(s) 186, 319, 559
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 3 cut(s) 143, 278, 785
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 3 cut(s) 91, 153, 700
BspACI CCGC 7 cut(s) 393, 574, 592, 629, 676, 738, 755
BspANI GGCC 3 cut(s) 143, 278, 785
BspCNI CTCAG 3 cut(s) 37, 113, 367
BspDI ATCGAT 1 cut(s) 152
BspFNI CGCG 4 cut(s) 391, 393, 629, 676
BspLI GGNNCC 1 cut(s) 702
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 86, 695, 708
BspT104I TTCGAA 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 4 cut(s) 186, 213, 603, 747
BssMI GATC 3 cut(s) 91, 153, 700
BssSI CACGAG 1 cut(s) 656
BssT1I CCWWGG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 656
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 668
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 405, 416, 560, 567
BstDEI CTNAG 3 cut(s) 45, 100, 375
BstDSI CCRYGG 2 cut(s) 603, 747
BstF5I GGATG 4 cut(s) 226, 279, 327, 433
BstFNI CGCG 4 cut(s) 391, 393, 629, 676
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 3 cut(s) 94, 156, 703
BstMBI GATC 3 cut(s) 91, 153, 700
BstMWI GCNNNNNNNGC 5 cut(s) 140, 571, 608, 668, 682
BstNSI RCATGY 1 cut(s) 589
BstSCI CCNGG 1 cut(s) 185
BstUI CGCG 4 cut(s) 391, 393, 629, 676
BstV1I GCAGC 3 cut(s) 506, 549, 739
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
Bsu15I ATCGAT 1 cut(s) 152
BsuRI GGCC 3 cut(s) 143, 278, 785
BsuTUI ATCGAT 1 cut(s) 152
BtgI CCRYGG 2 cut(s) 603, 747
BtrI CACGTC 1 cut(s) 768
BtsCI GGATG 4 cut(s) 226, 279, 327, 433
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 3 cut(s) 42, 786, 786
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 4 cut(s) 405, 416, 560, 567
CfoI GCGC 1 cut(s) 393
Cfr10I RCCGGY 1 cut(s) 558
ClaI ATCGAT 1 cut(s) 152
CseI GACGC 2 cut(s) 123, 674
Csp6I GTAC 1 cut(s) 247
CviAII CATG 2 cut(s) 131, 586
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 3 cut(s) 45, 100, 375
DpnI GATC 3 cut(s) 93, 155, 702
DpnII GATC 3 cut(s) 91, 153, 700
EaeI YGGCCR 3 cut(s) 141, 276, 783
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 2 cut(s) 134, 589
FaiI YATR 7 cut(s) 65, 132, 204, 387, 410, 587, 773
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FatI CATG 2 cut(s) 130, 585
FauI CCCGC 1 cut(s) 567
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 3 cut(s) 495, 563, 753
FokI GGATG 4 cut(s) 233, 266, 334, 440
Fsp4HI GCNGC 3 cut(s) 495, 563, 753
GlaI GCGC 1 cut(s) 392
GluI GCNGC 3 cut(s) 495, 563, 753
HaeIII GGCC 3 cut(s) 143, 278, 785
HapII CCGG 3 cut(s) 186, 319, 559
HgaI GACGC 2 cut(s) 123, 674
HhaI GCGC 1 cut(s) 393
Hin1II CATG 2 cut(s) 134, 589
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HinfI GANTC 6 cut(s) 98, 149, 365, 396, 553, 637
HpaII CCGG 3 cut(s) 186, 319, 559
HphI GGTGA 5 cut(s) 22, 62, 337, 361, 727
Hpy166II GTNNAC 2 cut(s) 10, 316
Hpy188I TCNGA 4 cut(s) 103, 232, 307, 723
Hpy188III TCNNGA 2 cut(s) 95, 476
Hpy8I GTNNAC 2 cut(s) 10, 316
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 1 cut(s) 725
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 1 cut(s) 767
HpyCH4V TGCA 6 cut(s) 4, 525, 547, 565, 589, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 571, 608, 668, 682
HpyF3I CTNAG 3 cut(s) 45, 100, 375
HpySE526I ACGT 1 cut(s) 767
Hsp92II CATG 2 cut(s) 134, 589
HspAI GCGC 1 cut(s) 391
KroI GCCGGC 1 cut(s) 558
KroNI GCCGGC 1 cut(s) 560
Kzo9I GATC 3 cut(s) 91, 153, 700
LmnI GCTCC 2 cut(s) 160, 294
LpnPI CCDG 6 cut(s) 199, 332, 385, 432, 572, 681
Lsp1109I GCAGC 3 cut(s) 506, 549, 739
LweI GCATC 1 cut(s) 589
MaeII ACGT 1 cut(s) 767
MaeIII GTNAC 3 cut(s) 28, 40, 164
MalI GATC 3 cut(s) 93, 155, 702
MboI GATC 3 cut(s) 91, 153, 700
MboII GAAGA 5 cut(s) 340, 356, 366, 479, 710
MfeI CAATTG 1 cut(s) 542
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 3 cut(s) 143, 278, 785
MluCI AATT 4 cut(s) 196, 498, 542, 705
MluNI TGGCCA 3 cut(s) 143, 278, 785
MlyI GAGTC 2 cut(s) 92, 359
MmeI TCCRAC 2 cut(s) 560, 746
MnlI CCTC 6 cut(s) 40, 78, 340, 474, 601, 721
Mox20I TGGCCA 3 cut(s) 143, 278, 785
MroNI GCCGGC 1 cut(s) 558
MscI TGGCCA 3 cut(s) 143, 278, 785
MseI TTAA 1 cut(s) 617
MslI CAYNNNNRTG 3 cut(s) 284, 413, 770
Msp20I TGGCCA 3 cut(s) 143, 278, 785
MspI CCGG 3 cut(s) 186, 319, 559
MspR9I CCNGG 1 cut(s) 187
MunI CAATTG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 611
MvnI CGCG 4 cut(s) 391, 393, 629, 676
MwoI GCNNNNNNNGC 5 cut(s) 140, 571, 608, 668, 682
NaeI GCCGGC 1 cut(s) 560
NciI CCSGG 1 cut(s) 187
NdeII GATC 3 cut(s) 91, 153, 700
NgoMIV GCCGGC 1 cut(s) 558
NlaIII CATG 2 cut(s) 134, 589
NlaIV GGNNCC 1 cut(s) 702
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 3 cut(s) 28, 40, 164
NspI RCATGY 1 cut(s) 589
NspV TTCGAA 1 cut(s) 238
PcsI WCGNNNNNNNCGW 2 cut(s) 641, 773
PctI GAATGC 1 cut(s) 611
PdiI GCCGGC 1 cut(s) 560
PfeI GAWTC 4 cut(s) 149, 396, 553, 637
PflFI GACNNNGTC 1 cut(s) 26
PkrI GCNGC 3 cut(s) 496, 564, 754
PleI GAGTC 2 cut(s) 92, 359
PpsI GAGTC 2 cut(s) 92, 359
PspN4I GGNNCC 1 cut(s) 702
PsuI RGATCY 1 cut(s) 700
PsyI GACNNNGTC 1 cut(s) 26
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 3 cut(s) 284, 413, 770
SaqAI TTAA 1 cut(s) 617
SatI GCNGC 3 cut(s) 495, 563, 753
Sau3AI GATC 3 cut(s) 91, 153, 700
SchI GAGTC 2 cut(s) 92, 359
ScrFI CCNGG 1 cut(s) 187
SduI GDGCHC 1 cut(s) 291
SetI ASST 5 cut(s) 70, 351, 571, 670, 770
SfaNI GCATC 1 cut(s) 589
SfuI TTCGAA 1 cut(s) 238
SmiMI CAYNNNNRTG 3 cut(s) 284, 413, 770
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
Sse9I AATT 4 cut(s) 196, 498, 542, 705
SsiI CCGC 7 cut(s) 393, 574, 592, 629, 676, 738, 755
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 1 cut(s) 770
TaqI TCGA 4 cut(s) 90, 147, 152, 238
TasI AATT 4 cut(s) 196, 498, 542, 705
TfiI GAWTC 4 cut(s) 149, 396, 553, 637
Tru1I TTAA 1 cut(s) 617
Tru9I TTAA 1 cut(s) 617
TscAI CASTG 3 cut(s) 49, 786, 793
TseFI GTSAC 3 cut(s) 28, 40, 164
TseI GCWGC 3 cut(s) 494, 562, 752
Tsp45I GTSAC 3 cut(s) 28, 40, 164
TspDTI ATGAA 3 cut(s) 237, 257, 483
TspGWI ACGGA 1 cut(s) 620
TspRI CASTG 3 cut(s) 49, 786, 793
Tth111I GACNNNGTC 1 cut(s) 26
XapI RAATTY 1 cut(s) 196
XceI RCATGY 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.