Prupe.1G150800_v2.0.a1

Adenylate isopentenyltransferase 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011736
Physical Location & Seq
Reverse (-)
11919572 .. 11921953
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G150800.1

Sequence Viewer

Length: 1017 bp
ATGAGAATTTTCATGCCTGCCTGCAAGCAAGGACAGCCTATGGTGAGCTTCCAAGGCCCTCCTGGCTTGCCCAGCATCAACATGGATCCTTTTTTCCGGCGAAAAGATAAGGTTGTTTTTGTAATGGGAGCCACCGGGACTGGCAAGTCCAGGCTTGCCATAGACCTCGCAACCTTTCACCGTGCTGAGATTATAAACTCGGACAAAATGCAAGTCTACAAGGGCTTGGACATCGTCACCAACAAAGTCACTGAAGAGGAGTGTAGAGGCATACCTCATCACTTGCTTGGACAAATAGATCCAAACTTCAACTTCACAGCCAATGATTTCAAGCAGCATGCATCCTTGGCTATCGAATCGATCTTGGAGCGCGACAGGCTCCCCATCATCGCCGGTGGCTCGAATTCCTACATCGAAGCTCTGGTGGACGACCACCCCGAGTTCAGAATGAAGTACGAGTGTTGCTTTATATGGGTAGATGTGGCGCTGCCGGTGCTCAACTCTTTTGTATCGGAGAGAGTGGATCGGATGGTGAAGGCGGGTTTGGTGGATGAGGTGAGGAGAATGTTCGATTCCACTGCTGAAGCAGAATATACTCACGGAATCAGGAAGGCTATAGGCATGCCTGAATTGGATGAGTATTTGCGGAGCGAGGCTTGTGGTATCAACGATGAGGAAACGCAAGAGAGGCTTCTTCAAACAGCTATTTCTAAAATTAAAGAAAACACTTGCATTTTGGCACACCGCCAATTGAAAAAGATTCGTCGGCTCTGCAGCCGACGAAACTGGAACATGCATCGCCTCGACGCCACAGAAGTATTCTTAAAGCACCGCGTGGAGGCTGATCAAGCATGGGAAAAACACGTGGCAAGACCCGCCAAGAGGATGGTTGGTAACTTCCTTGTAGACCCAATGCCCACAATTCTTCCATCCGACACTCTTGCCACCGCTACCACCACTACCACCACTACCGTTCTTGCCACATCTATGCCCGCCGTCGCCACCGCAACCCGTTAG

Protein Analysis

339

Amino Acids

38.19

Weight (kDa)

7.66

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011736)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23630 AT5G19040 AT5G19040 AT5G19040
fragaria_vesca FvH4_4g14440
malus_domestica MD13G1182800.v1.1 MD16G1183400.v1.1
prunus_persica Prupe.1G150800_v2.0.a1
pyrus_communis pycom13g15770 pycom16g15410
rosa_chinensis RchiOBHm_Chr4g0418401
rosa_laevigata RLG00000007870
rosa_multiflora Rmu_sc0001266.1_g000013
rosa_roxburghii Rroxscaffold_5G00361580
rosa_rugosa Rorug04G0152700
rosa_samantha Rh4BG216100 Rh4CG226100 Rh4DG212800
rosa_wichuraiana Rw4G018210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 194
AasI GACNNNNNNGTC 1 cut(s) 145
AccI GTMKAC 2 cut(s) 216, 906
AccII CGCG 2 cut(s) 372, 834
AciI CCGC 8 cut(s) 539, 646, 745, 832, 876, 948, 993, 1005
AclWI GGATC 4 cut(s) 80, 93, 293, 531
AcsI RAATTY 2 cut(s) 6, 403
AcuI CTGAAG 2 cut(s) 273, 603
AcvI CACGTG 1 cut(s) 865
AcyI GRCGYC 1 cut(s) 807
AdeI CACNNNGTG 1 cut(s) 835
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 838, 882
AflIII ACRYGT 1 cut(s) 862
AgsI TTSAA 4 cut(s) 310, 331, 698, 754
AjnI CCWGG 2 cut(s) 61, 149
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AluBI AGCT 3 cut(s) 48, 419, 704
AluI AGCT 3 cut(s) 48, 419, 704
Alw21I GWGCWC 1 cut(s) 498
AlwI GGATC 4 cut(s) 80, 93, 293, 531
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 3 cut(s) 334, 487, 774
ApoI RAATTY 2 cut(s) 6, 403
ArsI GACNNNNNNTTYG 2 cut(s) 198, 230
AspLEI GCGC 2 cut(s) 372, 487
AspS9I GGNCC 1 cut(s) 56
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 5 cut(s) 55, 170, 229, 544, 568
AvaI CYCGRG 1 cut(s) 437
BamHI GGATCC 1 cut(s) 85
BbrPI CACGTG 1 cut(s) 865
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 3 cut(s) 346, 474, 786
BccI CCATC 4 cut(s) 392, 523, 880, 937
BceAI ACGGC 1 cut(s) 980
BcgI CGANNNNNNTGC 4 cut(s) 560, 594, 923, 957
BciT130I CCWGG 2 cut(s) 63, 151
BclI TGATCA 1 cut(s) 844
BcnI CCSGG 1 cut(s) 136
BfmI CTRYAG 2 cut(s) 615, 772
BfoI RGCGCY 1 cut(s) 488
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 3 cut(s) 335, 488, 775
BlsI GCNGC 3 cut(s) 336, 489, 776
Bme1390I CCNGG 3 cut(s) 63, 136, 151
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 3 cut(s) 87, 130, 380
BmrFI CCNGG 3 cut(s) 63, 136, 151
BmsI GCATC 3 cut(s) 84, 350, 805
BpuMI CCSGG 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 359
BsaAI YACGTR 1 cut(s) 865
BsaHI GRCGYC 1 cut(s) 807
BsaJI CCNNGG 2 cut(s) 52, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 838, 882
Bse118I RCCGGY 2 cut(s) 392, 490
Bse1I ACTGG 2 cut(s) 145, 791
BseBI CCWGG 2 cut(s) 63, 151
BseCI ATCGAT 1 cut(s) 359
BseDI CCNNGG 2 cut(s) 52, 345
BseGI GGATG 6 cut(s) 341, 534, 556, 640, 891, 929
BseLI CCNNNNNNNGG 2 cut(s) 838, 882
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 2 cut(s) 145, 791
BseRI GAGGAG 2 cut(s) 272, 574
BseXI GCAGC 3 cut(s) 346, 474, 786
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 2 cut(s) 372, 834
BshFI GGCC 1 cut(s) 57
BshVI ATCGAT 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 498
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 4 cut(s) 97, 135, 393, 491
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 838, 882
BsmFI GGGAC 1 cut(s) 151
BsnI GGCC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 5 cut(s) 85, 298, 360, 523, 844
BspACI CCGC 8 cut(s) 539, 646, 745, 832, 876, 948, 993, 1005
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 178
BspDI ATCGAT 1 cut(s) 359
BspFNI CGCG 2 cut(s) 372, 834
BspLI GGNNCC 3 cut(s) 87, 130, 380
BspMAI CTGCAG 1 cut(s) 776
BspPI GGATC 4 cut(s) 80, 93, 293, 531
BsrFI RCCGGY 2 cut(s) 392, 490
BsrI ACTGG 2 cut(s) 145, 791
BssAI RCCGGY 2 cut(s) 392, 490
BssECI CCNNGG 2 cut(s) 52, 345
BssMI GATC 5 cut(s) 85, 298, 360, 523, 844
BssNI GRCGYC 1 cut(s) 807
BssT1I CCWWGG 2 cut(s) 52, 345
Bst2UI CCWGG 2 cut(s) 63, 151
Bst4CI ACNGT 2 cut(s) 182, 973
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 807
BstBAI YACGTR 1 cut(s) 865
BstC8I GCNNGC 8 cut(s) 18, 22, 26, 68, 156, 339, 623, 993
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 6 cut(s) 341, 534, 556, 640, 891, 929
BstFNI CGCG 2 cut(s) 372, 834
BstH2I RGCGCY 1 cut(s) 488
BstHHI GCGC 2 cut(s) 372, 487
BstKTI GATC 5 cut(s) 88, 301, 363, 526, 847
BstMBI GATC 5 cut(s) 85, 298, 360, 523, 844
BstNI CCWGG 2 cut(s) 63, 151
BstNSI RCATGY 3 cut(s) 341, 625, 796
BstSCI CCNGG 3 cut(s) 61, 134, 149
BstSFI CTRYAG 2 cut(s) 615, 772
BstUI CGCG 2 cut(s) 372, 834
BstV1I GCAGC 3 cut(s) 346, 474, 786
BstX2I RGATCY 2 cut(s) 85, 298
BstXI CCANNNNNNTGG 1 cut(s) 886
BstYI RGATCY 2 cut(s) 85, 298
Bsu15I ATCGAT 1 cut(s) 359
BsuRI GGCC 1 cut(s) 57
BsuTUI ATCGAT 1 cut(s) 359
BtgZI GCGATG 2 cut(s) 373, 782
BtsCI GGATG 6 cut(s) 341, 534, 556, 640, 891, 929
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 2 cut(s) 249, 576
Cac8I GCNNGC 8 cut(s) 18, 22, 26, 68, 156, 339, 623, 993
CfoI GCGC 2 cut(s) 372, 487
Cfr10I RCCGGY 2 cut(s) 392, 490
Cfr13I GGNCC 1 cut(s) 56
ClaI ATCGAT 1 cut(s) 359
CseI GACGC 1 cut(s) 815
Csp6I GTAC 1 cut(s) 454
CviAII CATG 6 cut(s) 13, 82, 338, 622, 793, 852
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 1 cut(s) 186
DpnI GATC 5 cut(s) 87, 300, 362, 525, 846
DpnII GATC 5 cut(s) 85, 298, 360, 523, 844
DraIII CACNNNGTG 1 cut(s) 835
DrdI GACNNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 52, 345
Eco57I CTGAAG 2 cut(s) 273, 603
Eco72I CACGTG 1 cut(s) 865
Eco88I CYCGRG 1 cut(s) 437
EcoO109I RGGNCCY 1 cut(s) 56
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 2 cut(s) 61, 149
EcoT14I CCWWGG 2 cut(s) 52, 345
EcoT22I ATGCAT 2 cut(s) 343, 798
ErhI CCWWGG 2 cut(s) 52, 345
FaeI CATG 6 cut(s) 16, 85, 341, 625, 796, 855
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FaqI GGGAC 1 cut(s) 151
FatI CATG 6 cut(s) 12, 81, 337, 621, 792, 851
FauI CCCGC 3 cut(s) 532, 883, 1000
FbaI TGATCA 1 cut(s) 844
FblI GTMKAC 2 cut(s) 216, 906
Fnu4HI GCNGC 3 cut(s) 335, 488, 775
FokI GGATG 6 cut(s) 328, 541, 563, 647, 898, 916
Fsp4HI GCNGC 3 cut(s) 335, 488, 775
GlaI GCGC 2 cut(s) 371, 486
GluI GCNGC 3 cut(s) 335, 488, 775
GsaI CCCAGC 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 488
HaeIII GGCC 1 cut(s) 57
HapII CCGG 4 cut(s) 97, 135, 393, 491
HgaI GACGC 1 cut(s) 815
HhaI GCGC 2 cut(s) 372, 487
Hin1I GRCGYC 1 cut(s) 807
Hin1II CATG 6 cut(s) 16, 85, 341, 625, 796, 855
Hin6I GCGC 2 cut(s) 370, 485
HinP1I GCGC 2 cut(s) 370, 485
HinfI GANTC 4 cut(s) 356, 572, 603, 760
HpaII CCGG 4 cut(s) 97, 135, 393, 491
HphI GGTGA 5 cut(s) 55, 170, 229, 544, 568
Hpy166II GTNNAC 3 cut(s) 217, 427, 907
Hpy188I TCNGA 5 cut(s) 202, 446, 514, 528, 934
Hpy188III TCNNGA 1 cut(s) 607
Hpy8I GTNNAC 3 cut(s) 217, 427, 907
Hpy99I CGWCG 4 cut(s) 768, 783, 809, 1001
HpyAV CCTTC 2 cut(s) 529, 604
HpyCH4III ACNGT 2 cut(s) 182, 973
HpyCH4IV ACGT 1 cut(s) 864
HpyCH4V TGCA 6 cut(s) 24, 211, 341, 732, 774, 796
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 864
Hsp92I GRCGYC 1 cut(s) 807
Hsp92II CATG 6 cut(s) 16, 85, 341, 625, 796, 855
HspAI GCGC 2 cut(s) 370, 485
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 5 cut(s) 85, 298, 360, 523, 844
LmnI GCTCC 4 cut(s) 128, 367, 384, 648
Lsp1109I GCAGC 3 cut(s) 346, 474, 786
LweI GCATC 3 cut(s) 84, 350, 805
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 3 cut(s) 235, 247, 893
MalI GATC 5 cut(s) 87, 300, 362, 525, 846
MboI GATC 5 cut(s) 85, 298, 360, 523, 844
MboII GAAGA 3 cut(s) 266, 686, 917
MfeI CAATTG 1 cut(s) 749
MflI RGATCY 2 cut(s) 85, 298
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 6 cut(s) 6, 403, 629, 714, 749, 921
MmeI TCCRAC 1 cut(s) 957
Mph1103I ATGCAT 2 cut(s) 343, 798
MseI TTAA 2 cut(s) 717, 824
MslI CAYNNNNRTG 2 cut(s) 80, 986
MspI CCGG 4 cut(s) 97, 135, 393, 491
MspR9I CCNGG 3 cut(s) 63, 136, 151
MunI CAATTG 1 cut(s) 749
MvaI CCWGG 2 cut(s) 63, 151
MvnI CGCG 2 cut(s) 372, 834
NciI CCSGG 1 cut(s) 136
NdeII GATC 5 cut(s) 85, 298, 360, 523, 844
NlaIII CATG 6 cut(s) 16, 85, 341, 625, 796, 855
NlaIV GGNNCC 3 cut(s) 87, 130, 380
NmuCI GTSAC 2 cut(s) 235, 247
NsiI ATGCAT 2 cut(s) 343, 798
NspI RCATGY 3 cut(s) 341, 625, 796
PaeI GCATGC 2 cut(s) 341, 625
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PfeI GAWTC 4 cut(s) 356, 572, 603, 760
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 3 cut(s) 336, 489, 776
PmaCI CACGTG 1 cut(s) 865
PmlI CACGTG 1 cut(s) 865
Ppu21I YACGTR 1 cut(s) 865
PsiI TTATAA 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 61, 149
PspCI CACGTG 1 cut(s) 865
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 61, 149
PspN4I GGNNCC 3 cut(s) 87, 130, 380
PspPI GGNCC 1 cut(s) 56
PstI CTGCAG 1 cut(s) 776
PsuI RGATCY 2 cut(s) 85, 298
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 2 cut(s) 80, 986
SaqAI TTAA 2 cut(s) 717, 824
SatI GCNGC 3 cut(s) 335, 488, 775
Sau3AI GATC 5 cut(s) 85, 298, 360, 523, 844
Sau96I GGNCC 1 cut(s) 56
ScrFI CCNGG 3 cut(s) 63, 136, 151
SduI GDGCHC 1 cut(s) 498
SetI ASST 9 cut(s) 50, 114, 168, 176, 277, 421, 558, 706, 867
SfaNI GCATC 3 cut(s) 84, 350, 805
SfcI CTRYAG 2 cut(s) 615, 772
SgrAI CRCCGGYG 1 cut(s) 392
SmiMI CAYNNNNRTG 2 cut(s) 80, 986
SphI GCATGC 2 cut(s) 341, 625
Sse9I AATT 6 cut(s) 6, 403, 629, 714, 749, 921
SsiI CCGC 8 cut(s) 539, 646, 745, 832, 876, 948, 993, 1005
StyD4I CCNGG 3 cut(s) 61, 134, 149
StyI CCWWGG 2 cut(s) 52, 345
TaaI ACNGT 2 cut(s) 182, 973
TaiI ACGT 1 cut(s) 867
TaqI TCGA 6 cut(s) 354, 359, 401, 414, 570, 804
TasI AATT 6 cut(s) 6, 403, 629, 714, 749, 921
TfiI GAWTC 4 cut(s) 356, 572, 603, 760
Tru1I TTAA 2 cut(s) 717, 824
Tru9I TTAA 2 cut(s) 717, 824
TscAI CASTG 2 cut(s) 256, 583
TseFI GTSAC 2 cut(s) 235, 247
TseI GCWGC 3 cut(s) 334, 487, 774
Tsp45I GTSAC 2 cut(s) 235, 247
TspDTI ATGAA 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 615
TspRI CASTG 2 cut(s) 256, 583
Tth111I GACNNNGTC 1 cut(s) 233
XapI RAATTY 2 cut(s) 6, 403
XceI RCATGY 3 cut(s) 341, 625, 796
XcmI CCANNNNNNNNNTGG 2 cut(s) 59, 79
XmiI GTMKAC 2 cut(s) 216, 906
Zsp2I ATGCAT 2 cut(s) 343, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.