Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0011736

Physical Location & Seq

Genomic Coordinates Forward (+)

43841622 .. 43842678

Gene Structure 1057 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ38839

Sequence Viewer

CDS Protein Promoter (2k)

Length: 993 bp

ATGCAAACTCTTTTCTCCATAGCTGCCTGCCAACAAGTAGTTCACATACAGCCGATGGTAAGATTCCAAAACCCGCCAGCCGGACTATCCATTGATCGATTTTTCAGTCGGAATAAGGATAAGGTTGTGATAGTAATGGGAGCCACGGGAACCGGAAAGTCCAGGCTTGCCATAGACCTTGCATTAAAATATTCGGCCGAGATCATAAACTCGGATAAAATGCAAGTCTATAAAGGTTTGGACACCCTCACCAACAAGGTCACTGAGGAGGAGTGCAAAGGCGTACCACATCACCTGTTGGGCACAGTGGATCCTGAGTCGAACTTCACAGTTAATGATTTTAAGCATCATGCGTCTTTGGCCATTGAATCGATCCTGGAGCGTGACCGGTTACCTATCATCGCCGGTGGGTCAAACTCGTACATAGAGGCACTGGTGGACGAGCACCCCGAGTTTCGGAGGAGGTACGAGTGCTGCTTTCTTTGGGTCGACGTAACGCCGCGGGTGCTCAACTCGTTTGTGTCGGAGCGAGTTGATCGGATGGTGGAGGTGGGTGGTCTGGTGGACGAGGTGAGGAGAATGTTCGACCCAACAGCCGACGAGGAGGACTATAGCACCGGGATTAAGAAAGCTATCGGAGTTCCAGAGATGCATCATTACTTGCGCGGCGAAGCGTGTGGCAACGATGAAGAGACTCAAGAAGCGCTGTTGATGGAAGCTATTTCGAACATCAAGAAGAATACTCGCACGTTGGCCCATCGCCAACTGCAAAAGATTCACCGGCTGCGGAGCAAGTGGAACTGGAGCATGCACCGCCTCGACGCCACCGAAGCTTTCTTGAACCGCCGTCGCGGCCGGGAGGGAGACCGAGTGTGGGAGAAGCAAGTTGCGAGACCCGCCACAGTAATCGTCAGTAAGTTCCTCGTAGACCCAATGTCAACCATTCTTCCTCATGACACTACCTTTTGGCTGCTGCAACACGGTAGATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009536 GO:0009824 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016765 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0052381 GO:0052622 GO:0052623 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko00908 ko01100 ko01110 map00908 map01100 map01110

Pfam Domains

Protein Families

IPPT

Protein Analysis

330

Amino Acids

37.74

Weight (kDa)

6.9

Isoelectric Point (pI)

47.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IPT	PF01745	41 - 149	3.5e-09	Isopentenyl transferase
IPPT	PF01715	75 - 149	5.3e-23	IPP transferase
IPPT	PF01715	152 - 257	2e-14	IPP transferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011736)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23630 AT5G19040 AT5G19040 AT5G19040
fragaria_vesca	FvH4_4g14440
malus_domestica	MD13G1182800.v1.1 MD16G1183400.v1.1
prunus_persica	Prupe.1G150800_v2.0.a1
pyrus_communis	pycom13g15770 pycom16g15410
rosa_chinensis	RchiOBHm_Chr4g0418401
rosa_laevigata	RLG00000007870
rosa_multiflora	Rmu_sc0001266.1_g000013
rosa_roxburghii	Rroxscaffold_5G00361580
rosa_rugosa	Rorug04G0152700
rosa_samantha	Rh4BG216100 Rh4CG226100 Rh4DG212800
rosa_wichuraiana	Rw4G018210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	489, 927
AccII	CGCG	3 cut(s)	502, 666, 852
AciI	CCGC	9 cut(s)	74, 500, 502, 666, 787, 814, 844, 852, 897
AclWI	GGATC	3 cut(s)	305, 318, 367
AcoI	YGGCCR	3 cut(s)	195, 360, 853
AcyI	GRCGYC	1 cut(s)	822
AfaI	GTAC	3 cut(s)	285, 422, 467
AfeI	AGCGCT	1 cut(s)	705
AfiI	CCNNNNNNNGG	3 cut(s)	80, 456, 874
AgeI	ACCGGT	1 cut(s)	387
AgsI	TTSAA	2 cut(s)	368, 841
AhdI	GACNNNNNGTC	1 cut(s)	934
AjnI	CCWGG	2 cut(s)	161, 375
AjuI	GAANNNNNNNTTGG	2 cut(s)	24, 56
AluBI	AGCT	4 cut(s)	23, 632, 719, 833
AluI	AGCT	4 cut(s)	23, 632, 719, 833
Alw21I	GWGCWC	2 cut(s)	447, 510
Alw26I	GTCTC	3 cut(s)	686, 858, 886
AlwI	GGATC	3 cut(s)	305, 318, 367
Ama87I	CYCGRG	1 cut(s)	449
Aor51HI	AGCGCT	1 cut(s)	705
AoxI	GGCC	4 cut(s)	195, 360, 753, 853
ApeKI	GCWGC	5 cut(s)	23, 474, 784, 970, 973
AsiGI	ACCGGT	1 cut(s)	387
AspLEI	GCGC	2 cut(s)	666, 706
AspS9I	GGNCC	1 cut(s)	754
AsuC2I	CCSGG	2 cut(s)	619, 857
AsuHPI	GGTGA	4 cut(s)	241, 284, 583, 770
AsuII	TTCGAA	1 cut(s)	725
AvaI	CYCGRG	1 cut(s)	449
BaeGI	GKGCMC	1 cut(s)	305
BalI	TGGCCA	1 cut(s)	362
BamHI	GGATCC	1 cut(s)	310
Bbv12I	GWGCWC	2 cut(s)	447, 510
BbvI	GCAGC	5 cut(s)	10, 461, 771, 957, 960
BccI	CCATC	4 cut(s)	49, 535, 706, 765
BceAI	ACGGC	1 cut(s)	831
BciT130I	CCWGG	2 cut(s)	163, 377
BcnI	CCSGG	2 cut(s)	619, 857
BcoDI	GTCTC	3 cut(s)	686, 858, 886
BfmI	CTRYAG	1 cut(s)	610
BfoI	RGCGCY	1 cut(s)	707
BglI	GCCNNNNNGGC	1 cut(s)	852
BisI	GCNGC	8 cut(s)	24, 475, 500, 667, 785, 853, 971, 974
BlsI	GCNGC	8 cut(s)	25, 476, 501, 668, 786, 854, 972, 975
Bme1390I	CCNGG	4 cut(s)	163, 377, 619, 857
BmeRI	GACNNNNNGTC	1 cut(s)	934
BmeT110I	CYCGRG	1 cut(s)	449
BmgT120I	GGNCC	1 cut(s)	754
BmiI	GGNNCC	3 cut(s)	142, 151, 312
BmrFI	CCNGG	4 cut(s)	163, 377, 619, 857
BmsI	GCATC	3 cut(s)	355, 639, 661
BpmI	CTGGAG	2 cut(s)	398, 823
Bpu14I	TTCGAA	1 cut(s)	725
BpuEI	CTTGAG	1 cut(s)	681
BpuMI	CCSGG	2 cut(s)	619, 857
Bsa29I	ATCGAT	2 cut(s)	97, 371
BsaHI	GRCGYC	1 cut(s)	822
BsaI	GGTCTC	2 cut(s)	858, 886
BsaJI	CCNNGG	2 cut(s)	144, 500
BsaWI	WCCGGW	2 cut(s)	152, 387
BsaXI	ACNNNNNCTCC	2 cut(s)	855, 885
Bsc4I	CCNNNNNNNGG	3 cut(s)	80, 456, 874
Bse118I	RCCGGY	3 cut(s)	387, 404, 780
Bse1I	ACTGG	2 cut(s)	438, 806
BseBI	CCWGG	2 cut(s)	163, 377
BseCI	ATCGAT	2 cut(s)	97, 371
BseDI	CCNNGG	2 cut(s)	144, 500
BseGI	GGATG	1 cut(s)	546
BseLI	CCNNNNNNNGG	3 cut(s)	80, 456, 874
BseMII	CTCAG	2 cut(s)	255, 306
BseNI	ACTGG	2 cut(s)	438, 806
BseRI	GAGGAG	5 cut(s)	281, 284, 475, 589, 617
BseSI	GKGCMC	1 cut(s)	305
BseX3I	CGGCCG	2 cut(s)	195, 853
BseXI	GCAGC	5 cut(s)	10, 461, 771, 957, 960
Bsh1236I	CGCG	3 cut(s)	502, 666, 852
Bsh1285I	CGRYCG	2 cut(s)	198, 856
BshFI	GGCC	4 cut(s)	197, 362, 755, 855
BshTI	ACCGGT	1 cut(s)	387
BshVI	ATCGAT	2 cut(s)	97, 371
BsiEI	CGRYCG	2 cut(s)	198, 856
BsiHKAI	GWGCWC	2 cut(s)	447, 510
BsiHKCI	CYCGRG	1 cut(s)	449
BsiSI	CCGG	7 cut(s)	81, 153, 388, 405, 618, 781, 856
BslI	CCNNNNNNNGG	3 cut(s)	80, 456, 874
BsmAI	GTCTC	3 cut(s)	686, 858, 886
BsnI	GGCC	4 cut(s)	197, 362, 755, 855
Bso31I	GGTCTC	2 cut(s)	858, 886
BsoBI	CYCGRG	1 cut(s)	449
Bsp119I	TTCGAA	1 cut(s)	725
Bsp1286I	GDGCHC	3 cut(s)	305, 447, 510
Bsp143I	GATC	5 cut(s)	94, 201, 310, 372, 535
BspACI	CCGC	9 cut(s)	74, 500, 502, 666, 787, 814, 844, 852, 897
BspANI	GGCC	4 cut(s)	197, 362, 755, 855
BspCNI	CTCAG	2 cut(s)	256, 307
BspDI	ATCGAT	2 cut(s)	97, 371
BspFNI	CGCG	3 cut(s)	502, 666, 852
BspHI	TCATGA	1 cut(s)	952
BspLI	GGNNCC	3 cut(s)	142, 151, 312
BspPI	GGATC	3 cut(s)	305, 318, 367
BspT104I	TTCGAA	1 cut(s)	725
BspTNI	GGTCTC	2 cut(s)	858, 886
BsrFI	RCCGGY	3 cut(s)	387, 404, 780
BsrI	ACTGG	2 cut(s)	438, 806
BssAI	RCCGGY	3 cut(s)	387, 404, 780
BssECI	CCNNGG	2 cut(s)	144, 500
BssMI	GATC	5 cut(s)	94, 201, 310, 372, 535
BssNI	GRCGYC	1 cut(s)	822
Bst2UI	CCWGG	2 cut(s)	163, 377
Bst4CI	ACNGT	4 cut(s)	307, 331, 904, 983
Bst6I	CTCTTC	1 cut(s)	684
BstACI	GRCGYC	1 cut(s)	822
BstBI	TTCGAA	1 cut(s)	725
BstC8I	GCNNGC	4 cut(s)	28, 78, 168, 809
BstDEI	CTNAG	2 cut(s)	264, 315
BstDSI	CCRYGG	2 cut(s)	144, 500
BstEII	GGTNACC	1 cut(s)	390
BstF5I	GGATG	1 cut(s)	546
BstFNI	CGCG	3 cut(s)	502, 666, 852
BstH2I	RGCGCY	1 cut(s)	707
BstHHI	GCGC	2 cut(s)	666, 706
BstKTI	GATC	5 cut(s)	97, 204, 313, 375, 538
BstMAI	GTCTC	3 cut(s)	686, 858, 886
BstMBI	GATC	5 cut(s)	94, 201, 310, 372, 535
BstMCI	CGRYCG	2 cut(s)	198, 856
BstMWI	GCNNNNNNNGC	6 cut(s)	359, 505, 813, 830, 852, 896
BstNI	CCWGG	2 cut(s)	163, 377
BstNSI	RCATGY	1 cut(s)	811
BstPI	GGTNACC	1 cut(s)	390
BstSCI	CCNGG	4 cut(s)	161, 375, 617, 855
BstSFI	CTRYAG	1 cut(s)	610
BstSLI	GKGCMC	1 cut(s)	305
BstUI	CGCG	3 cut(s)	502, 666, 852
BstV1I	GCAGC	5 cut(s)	10, 461, 771, 957, 960
BstX2I	RGATCY	1 cut(s)	310
BstYI	RGATCY	1 cut(s)	310
BstZI	CGGCCG	2 cut(s)	195, 853
Bsu15I	ATCGAT	2 cut(s)	97, 371
BsuRI	GGCC	4 cut(s)	197, 362, 755, 855
BsuTUI	ATCGAT	2 cut(s)	97, 371
BtgI	CCRYGG	2 cut(s)	144, 500
BtgZI	GCGATG	2 cut(s)	385, 743
BtsCI	GGATG	1 cut(s)	546
BtsIMutI	CAGTG	3 cut(s)	261, 312, 431
Cac8I	GCNNGC	4 cut(s)	28, 78, 168, 809
CciI	TCATGA	1 cut(s)	952
CfoI	GCGC	2 cut(s)	666, 706
Cfr10I	RCCGGY	3 cut(s)	387, 404, 780
Cfr13I	GGNCC	1 cut(s)	754
Cfr42I	CCGCGG	1 cut(s)	503
ClaI	ATCGAT	2 cut(s)	97, 371
CseI	GACGC	2 cut(s)	342, 830
Csp6I	GTAC	3 cut(s)	284, 421, 466
CspAI	ACCGGT	1 cut(s)	387
CviAII	CATG	3 cut(s)	350, 808, 953
CviQI	GTAC	3 cut(s)	284, 421, 466
DdeI	CTNAG	2 cut(s)	264, 315
DpnI	GATC	5 cut(s)	96, 203, 312, 374, 537
DpnII	GATC	5 cut(s)	94, 201, 310, 372, 535
DriI	GACNNNNNGTC	1 cut(s)	934
EaeI	YGGCCR	3 cut(s)	195, 360, 853
EagI	CGGCCG	2 cut(s)	195, 853
Eam1104I	CTCTTC	1 cut(s)	684
Eam1105I	GACNNNNNGTC	1 cut(s)	934
EarI	CTCTTC	1 cut(s)	684
EclXI	CGGCCG	2 cut(s)	195, 853
Eco31I	GGTCTC	2 cut(s)	858, 886
Eco47III	AGCGCT	1 cut(s)	705
Eco52I	CGGCCG	2 cut(s)	195, 853
Eco88I	CYCGRG	1 cut(s)	449
Eco91I	GGTNACC	1 cut(s)	390
EcoO65I	GGTNACC	1 cut(s)	390
EcoRII	CCWGG	2 cut(s)	161, 375
EcoT22I	ATGCAT	1 cut(s)	654
FaeI	CATG	3 cut(s)	353, 811, 956
FatI	CATG	3 cut(s)	349, 807, 952
FauI	CCCGC	3 cut(s)	81, 495, 904
FblI	GTMKAC	2 cut(s)	489, 927
Fnu4HI	GCNGC	8 cut(s)	24, 475, 500, 667, 785, 853, 971, 974
FokI	GGATG	1 cut(s)	553
Fsp4HI	GCNGC	8 cut(s)	24, 475, 500, 667, 785, 853, 971, 974
GlaI	GCGC	2 cut(s)	665, 705
GluI	GCNGC	8 cut(s)	24, 475, 500, 667, 785, 853, 971, 974
GsuI	CTGGAG	2 cut(s)	398, 823
HaeII	RGCGCY	1 cut(s)	707
HaeIII	GGCC	4 cut(s)	197, 362, 755, 855
HapII	CCGG	7 cut(s)	81, 153, 388, 405, 618, 781, 856
HgaI	GACGC	2 cut(s)	342, 830
HhaI	GCGC	2 cut(s)	666, 706
Hin1I	GRCGYC	1 cut(s)	822
Hin1II	CATG	3 cut(s)	353, 811, 956
Hin6I	GCGC	2 cut(s)	664, 704
HinP1I	GCGC	2 cut(s)	664, 704
HincII	GTYRAC	2 cut(s)	490, 939
HindII	GTYRAC	2 cut(s)	490, 939
HindIII	AAGCTT	1 cut(s)	831
HinfI	GANTC	5 cut(s)	63, 317, 368, 694, 775
HpaII	CCGG	7 cut(s)	81, 153, 388, 405, 618, 781, 856
HphI	GGTGA	4 cut(s)	241, 284, 583, 770
Hpy166II	GTNNAC	6 cut(s)	43, 439, 490, 565, 928, 939
Hpy188I	TCNGA	6 cut(s)	111, 214, 459, 526, 540, 638
Hpy188III	TCNNGA	6 cut(s)	314, 644, 698, 733, 838, 953
Hpy8I	GTNNAC	6 cut(s)	43, 439, 490, 565, 928, 939
Hpy99I	CGWCG	4 cut(s)	494, 602, 824, 852
HpyCH4III	ACNGT	4 cut(s)	307, 331, 904, 983
HpyCH4IV	ACGT	2 cut(s)	492, 749
HpyCH4V	TGCA	8 cut(s)	4, 182, 223, 276, 652, 769, 811, 976
HpyF10VI	GCNNNNNNNGC	6 cut(s)	359, 505, 813, 830, 852, 896
HpyF3I	CTNAG	2 cut(s)	264, 315
HpySE526I	ACGT	2 cut(s)	492, 749
Hsp92I	GRCGYC	1 cut(s)	822
Hsp92II	CATG	3 cut(s)	353, 811, 956
HspAI	GCGC	2 cut(s)	664, 704
KspI	CCGCGG	1 cut(s)	503
Kzo9I	GATC	5 cut(s)	94, 201, 310, 372, 535
LmnI	GCTCC	5 cut(s)	140, 379, 526, 789, 804
Lsp1109I	GCAGC	5 cut(s)	10, 461, 771, 957, 960
LweI	GCATC	3 cut(s)	355, 639, 661
MaeII	ACGT	2 cut(s)	492, 749
MaeIII	GTNAC	4 cut(s)	259, 383, 390, 493
MalI	GATC	5 cut(s)	96, 203, 312, 374, 537
MboI	GATC	5 cut(s)	94, 201, 310, 372, 535
MboII	GAAGA	3 cut(s)	701, 748, 938
MflI	RGATCY	1 cut(s)	310
MhlI	GDGCHC	3 cut(s)	305, 447, 510
MlsI	TGGCCA	1 cut(s)	362
MluNI	TGGCCA	1 cut(s)	362
MlyI	GAGTC	2 cut(s)	326, 688
MmeI	TCCRAC	2 cut(s)	89, 504
Mox20I	TGGCCA	1 cut(s)	362
Mph1103I	ATGCAT	1 cut(s)	654
MscI	TGGCCA	1 cut(s)	362
MseI	TTAA	4 cut(s)	185, 333, 342, 624
Msp20I	TGGCCA	1 cut(s)	362
MspA1I	CMGCKG	1 cut(s)	502
MspI	CCGG	7 cut(s)	81, 153, 388, 405, 618, 781, 856
MspR9I	CCNGG	4 cut(s)	163, 377, 619, 857
MvaI	CCWGG	2 cut(s)	163, 377
MvnI	CGCG	3 cut(s)	502, 666, 852
MwoI	GCNNNNNNNGC	6 cut(s)	359, 505, 813, 830, 852, 896
NciI	CCSGG	2 cut(s)	619, 857
NdeII	GATC	5 cut(s)	94, 201, 310, 372, 535
NlaIII	CATG	3 cut(s)	353, 811, 956
NlaIV	GGNNCC	3 cut(s)	142, 151, 312
NmeAIII	GCCGAG	1 cut(s)	223
NmuCI	GTSAC	2 cut(s)	259, 383
NsiI	ATGCAT	1 cut(s)	654
NspI	RCATGY	1 cut(s)	811
NspV	TTCGAA	1 cut(s)	725
PaeI	GCATGC	1 cut(s)	811
PagI	TCATGA	1 cut(s)	952
PcsI	WCGNNNNNNNCGW	2 cut(s)	447, 825
PfeI	GAWTC	3 cut(s)	63, 368, 775
PfoI	TCCNGGA	1 cut(s)	375
PinAI	ACCGGT	1 cut(s)	387
PkrI	GCNGC	8 cut(s)	25, 476, 501, 668, 786, 854, 972, 975
PleI	GAGTC	2 cut(s)	325, 688
PpsI	GAGTC	2 cut(s)	325, 688
Psp6I	CCWGG	2 cut(s)	161, 375
PspEI	GGTNACC	1 cut(s)	390
PspGI	CCWGG	2 cut(s)	161, 375
PspN4I	GGNNCC	3 cut(s)	142, 151, 312
PspPI	GGNCC	1 cut(s)	754
PsuI	RGATCY	1 cut(s)	310
RsaI	GTAC	3 cut(s)	285, 422, 467
RsaNI	GTAC	3 cut(s)	284, 421, 466
SacII	CCGCGG	1 cut(s)	503
SalI	GTCGAC	1 cut(s)	488
SaqAI	TTAA	4 cut(s)	185, 333, 342, 624
SatI	GCNGC	8 cut(s)	24, 475, 500, 667, 785, 853, 971, 974
Sau3AI	GATC	5 cut(s)	94, 201, 310, 372, 535
Sau96I	GGNCC	1 cut(s)	754
SchI	GAGTC	2 cut(s)	326, 688
ScrFI	CCNGG	4 cut(s)	163, 377, 619, 857
SduI	GDGCHC	3 cut(s)	305, 447, 510
SfaNI	GCATC	3 cut(s)	355, 639, 661
SfcI	CTRYAG	1 cut(s)	610
Sfr303I	CCGCGG	1 cut(s)	503
SfuI	TTCGAA	1 cut(s)	725
SgrAI	CRCCGGYG	1 cut(s)	404
SgrBI	CCGCGG	1 cut(s)	503
SmlI	CTYRAG	1 cut(s)	696
SmoI	CTYRAG	1 cut(s)	696
SphI	GCATGC	1 cut(s)	811
SsiI	CCGC	9 cut(s)	74, 500, 502, 666, 787, 814, 844, 852, 897
SspI	AATATT	1 cut(s)	191
StyD4I	CCNGG	4 cut(s)	161, 375, 617, 855
TaaI	ACNGT	4 cut(s)	307, 331, 904, 983
TaiI	ACGT	2 cut(s)	495, 752
TaqI	TCGA	7 cut(s)	97, 320, 371, 489, 585, 725, 819
TaqII	GACCGA	1 cut(s)	882
TauI	GCSGC	3 cut(s)	502, 669, 855
TfiI	GAWTC	3 cut(s)	63, 368, 775
Tru1I	TTAA	4 cut(s)	185, 333, 342, 624
Tru9I	TTAA	4 cut(s)	185, 333, 342, 624
TscAI	CASTG	3 cut(s)	268, 312, 438
TseFI	GTSAC	2 cut(s)	259, 383
TseI	GCWGC	5 cut(s)	23, 474, 784, 970, 973
Tsp45I	GTSAC	2 cut(s)	259, 383
TspDTI	ATGAA	1 cut(s)	702
TspRI	CASTG	3 cut(s)	268, 312, 438
XceI	RCATGY	1 cut(s)	811
XmiI	GTMKAC	2 cut(s)	489, 927
Zsp2I	ATGCAT	1 cut(s)	654

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.