RLG00000007870

Adenylate isopentenyltransferase 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011736
Physical Location & Seq
Reverse (-)
22227315 .. 22228355
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007870

Sequence Viewer

Length: 1041 bp
ATGGCTGCCTGCCAACAAGTAGTTCACATACAGCCGACAGTAAGATTCCAAAACCCCCCAGCCGGACTATCCATTGATCGATTTTTCAGTCGGAATAAGGATAAGGTTGTGATAGTAATGGGAGCCACGGGAACCGGTAAGTCCAGGCTTGCCATAGACCTTGCATTAAAATATTCGGCCGAGATCATAAACTCGGATAAAATGCAAGTCTACAAAGGTTTGGACACCCTCACGAACAAGGTCACTGAGGAGGAGTGCAAAGGCGTACCACATCACCTGTTGGGCACAGTGGATCCGGAGTCGAACTTCACAGCTAATGATTTTAAGCATCATGCGTCTTTGGCCATTGAATCGATCCTGGAGCGTGACCGGTTACCTATCATCGCCGGTGGGTCAAACTCGTACATAGAGGCACTGGTGGACGAGCACCCCGAGTTTCGGAGGAGGTACGAGTGCTGCTTTCTGTGGGTCGACGTAACGCCGCGGGTGCTCAACTCGTTTGTGTCGGAGCGAGTTGATCGGATGGTGGAGGTGGGTGGTCTGGTGGACGAGGTGAGGAGAATGTTCGACCCAACAGCCGACGAGGAGGACTATAGCACCGGGATTAAGAAAGCTATCGGAGTTCCAGAGATGCATCATTACTTGCGCGGCGAAGCGTGTGGCAACGATGAAGAGACTCAAGAAGCGCTGTTGTTGGAAGCCATTTCGAACATCAAGAAGAATACTCGCACGTTGGCCCATCGCCAACTGCAAAAGATTCACCGGCTGCAGAGCAAGTGGAACTGGAGCATGCACCGCCTCGACGCCACCGAAGCTTTCTTGAACCGCCGTCGAGGCCGGGAGGGAGACAGAGTGTGGGAGAAGCACGTTGCGAGACCCGCCACAGGAATCGTCAGTAAGTTCCTCGTAGACCCAATGTCAACCATTCTTCCTCATGACACTGCCTTTGTAGCTGTAGCGGCTACGGCCGGCAGCACCAGCACCACCAGTGTCAATTCTCTTGCGGTGTCCATGCCGGCCGTGGCTGCTGCAACACGGTAG

Protein Analysis

347

Amino Acids

38.62

Weight (kDa)

6.68

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPT PF01745 35 - 143 4.9e-10 Isopentenyl transferase
IPPT PF01715 69 - 144 9.4e-23 IPP transferase
IPPT PF01715 146 - 251 3.2e-14 IPP transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011736)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23630 AT5G19040 AT5G19040 AT5G19040
fragaria_vesca FvH4_4g14440
malus_domestica MD13G1182800.v1.1 MD16G1183400.v1.1
prunus_persica Prupe.1G150800_v2.0.a1
pyrus_communis pycom13g15770 pycom16g15410
rosa_chinensis RchiOBHm_Chr4g0418401
rosa_laevigata RLG00000007870
rosa_multiflora Rmu_sc0001266.1_g000013
rosa_roxburghii Rroxscaffold_5G00361580
rosa_rugosa Rorug04G0152700
rosa_samantha Rh4BG216100 Rh4CG226100 Rh4DG212800
rosa_wichuraiana Rw4G018210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 210, 471, 909
AccII CGCG 2 cut(s) 484, 648
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 8 cut(s) 482, 484, 648, 796, 826, 879, 959, 1004
AclWI GGATC 3 cut(s) 287, 300, 349
AcoI YGGCCR 4 cut(s) 177, 342, 966, 1017
AcyI GRCGYC 1 cut(s) 804
AfaI GTAC 3 cut(s) 267, 404, 449
AfeI AGCGCT 1 cut(s) 687
AfiI CCNNNNNNNGG 3 cut(s) 62, 438, 884
AgeI ACCGGT 2 cut(s) 134, 369
AgsI TTSAA 2 cut(s) 350, 823
AhdI GACNNNNNGTC 1 cut(s) 916
AjnI CCWGG 2 cut(s) 143, 357
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 4 cut(s) 314, 614, 815, 953
AluI AGCT 4 cut(s) 314, 614, 815, 953
Alw21I GWGCWC 2 cut(s) 429, 492
Alw26I GTCTC 3 cut(s) 668, 840, 868
AlwI GGATC 3 cut(s) 287, 300, 349
Ama87I CYCGRG 1 cut(s) 431
Aor13HI TCCGGA 1 cut(s) 295
Aor51HI AGCGCT 1 cut(s) 687
AoxI GGCC 6 cut(s) 177, 342, 735, 835, 966, 1017
ApeKI GCWGC 6 cut(s) 5, 456, 766, 972, 1025, 1028
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
AsiGI ACCGGT 2 cut(s) 134, 369
AspLEI GCGC 2 cut(s) 648, 688
AspS9I GGNCC 1 cut(s) 736
AsuC2I CCSGG 2 cut(s) 601, 839
AsuHPI GGTGA 3 cut(s) 266, 565, 752
AsuII TTCGAA 1 cut(s) 707
AvaI CYCGRG 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 344
BamHI GGATCC 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 429, 492
BbvI GCAGC 5 cut(s) 443, 753, 984, 1012, 1015
BccI CCATC 2 cut(s) 517, 747
BceAI ACGGC 3 cut(s) 813, 981, 1004
BciT130I CCWGG 2 cut(s) 145, 359
BcnI CCSGG 2 cut(s) 601, 839
BcoDI GTCTC 3 cut(s) 668, 840, 868
BfmI CTRYAG 3 cut(s) 592, 767, 954
BfoI RGCGCY 1 cut(s) 689
BglI GCCNNNNNGGC 1 cut(s) 834
BisI GCNGC 9 cut(s) 6, 457, 482, 649, 767, 960, 973, 1026, 1029
BlsI GCNGC 9 cut(s) 7, 458, 483, 650, 768, 961, 974, 1027, 1030
Bme1390I CCNGG 4 cut(s) 145, 359, 601, 839
BmeRI GACNNNNNGTC 1 cut(s) 916
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 1 cut(s) 736
BmiI GGNNCC 3 cut(s) 124, 133, 294
BmrFI CCNGG 4 cut(s) 145, 359, 601, 839
BmsI GCATC 3 cut(s) 337, 621, 643
BpmI CTGGAG 2 cut(s) 380, 805
Bpu14I TTCGAA 1 cut(s) 707
BpuEI CTTGAG 1 cut(s) 663
BpuMI CCSGG 2 cut(s) 601, 839
Bsa29I ATCGAT 2 cut(s) 79, 353
BsaHI GRCGYC 1 cut(s) 804
BsaI GGTCTC 1 cut(s) 868
BsaJI CCNNGG 3 cut(s) 126, 482, 1020
BsaWI WCCGGW 3 cut(s) 134, 295, 369
BsaXI ACNNNNNCTCC 2 cut(s) 837, 867
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 438, 884
Bse118I RCCGGY 6 cut(s) 134, 369, 386, 762, 968, 1015
Bse1I ACTGG 3 cut(s) 420, 788, 987
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 2 cut(s) 145, 359
BseCI ATCGAT 2 cut(s) 79, 353
BseDI CCNNGG 3 cut(s) 126, 482, 1020
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 3 cut(s) 62, 438, 884
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 420, 788, 987
BseRI GAGGAG 5 cut(s) 263, 266, 457, 571, 599
BseSI GKGCMC 1 cut(s) 287
BseX3I CGGCCG 3 cut(s) 177, 966, 1017
BseXI GCAGC 5 cut(s) 443, 753, 984, 1012, 1015
BseYI CCCAGC 1 cut(s) 58
Bsh1236I CGCG 2 cut(s) 484, 648
Bsh1285I CGRYCG 3 cut(s) 180, 969, 1020
BshFI GGCC 6 cut(s) 179, 344, 737, 837, 968, 1019
BshTI ACCGGT 2 cut(s) 134, 369
BshVI ATCGAT 2 cut(s) 79, 353
BsiEI CGRYCG 3 cut(s) 180, 969, 1020
BsiHKAI GWGCWC 2 cut(s) 429, 492
BsiHKCI CYCGRG 1 cut(s) 431
BslI CCNNNNNNNGG 3 cut(s) 62, 438, 884
BsmAI GTCTC 3 cut(s) 668, 840, 868
BsnI GGCC 6 cut(s) 179, 344, 737, 837, 968, 1019
Bso31I GGTCTC 1 cut(s) 868
BsoBI CYCGRG 1 cut(s) 431
Bsp119I TTCGAA 1 cut(s) 707
Bsp1286I GDGCHC 3 cut(s) 287, 429, 492
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 5 cut(s) 76, 183, 292, 354, 517
BspACI CCGC 8 cut(s) 482, 484, 648, 796, 826, 879, 959, 1004
BspANI GGCC 6 cut(s) 179, 344, 737, 837, 968, 1019
BspCNI CTCAG 1 cut(s) 238
BspDI ATCGAT 2 cut(s) 79, 353
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 2 cut(s) 484, 648
BspHI TCATGA 1 cut(s) 934
BspLI GGNNCC 3 cut(s) 124, 133, 294
BspMAI CTGCAG 1 cut(s) 771
BspPI GGATC 3 cut(s) 287, 300, 349
BspT104I TTCGAA 1 cut(s) 707
BspTNI GGTCTC 1 cut(s) 868
BsrFI RCCGGY 6 cut(s) 134, 369, 386, 762, 968, 1015
BsrI ACTGG 3 cut(s) 420, 788, 987
BssAI RCCGGY 6 cut(s) 134, 369, 386, 762, 968, 1015
BssECI CCNNGG 3 cut(s) 126, 482, 1020
BssMI GATC 5 cut(s) 76, 183, 292, 354, 517
BssNI GRCGYC 1 cut(s) 804
Bst2UI CCWGG 2 cut(s) 145, 359
Bst4CI ACNGT 3 cut(s) 40, 289, 1038
Bst6I CTCTTC 1 cut(s) 666
BstACI GRCGYC 1 cut(s) 804
BstBI TTCGAA 1 cut(s) 707
BstC8I GCNNGC 5 cut(s) 10, 150, 791, 970, 1017
BstDEI CTNAG 1 cut(s) 246
BstDSI CCRYGG 3 cut(s) 126, 482, 1020
BstEII GGTNACC 1 cut(s) 372
BstF5I GGATG 1 cut(s) 528
BstFNI CGCG 2 cut(s) 484, 648
BstH2I RGCGCY 1 cut(s) 689
BstHHI GCGC 2 cut(s) 648, 688
BstKTI GATC 5 cut(s) 79, 186, 295, 357, 520
BstMAI GTCTC 3 cut(s) 668, 840, 868
BstMBI GATC 5 cut(s) 76, 183, 292, 354, 517
BstMCI CGRYCG 3 cut(s) 180, 969, 1020
BstNI CCWGG 2 cut(s) 145, 359
BstNSI RCATGY 1 cut(s) 793
BstPI GGTNACC 1 cut(s) 372
BstSCI CCNGG 4 cut(s) 143, 357, 599, 837
BstSFI CTRYAG 3 cut(s) 592, 767, 954
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 2 cut(s) 484, 648
BstV1I GCAGC 5 cut(s) 443, 753, 984, 1012, 1015
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BstZI CGGCCG 3 cut(s) 177, 966, 1017
Bsu15I ATCGAT 2 cut(s) 79, 353
BsuRI GGCC 6 cut(s) 179, 344, 737, 837, 968, 1019
BsuTUI ATCGAT 2 cut(s) 79, 353
BtgI CCRYGG 3 cut(s) 126, 482, 1020
BtgZI GCGATG 2 cut(s) 367, 725
BtsCI GGATG 1 cut(s) 528
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 5 cut(s) 243, 294, 413, 939, 994
Cac8I GCNNGC 5 cut(s) 10, 150, 791, 970, 1017
CciI TCATGA 1 cut(s) 934
CfoI GCGC 2 cut(s) 648, 688
Cfr10I RCCGGY 6 cut(s) 134, 369, 386, 762, 968, 1015
Cfr13I GGNCC 1 cut(s) 736
Cfr42I CCGCGG 1 cut(s) 485
ClaI ATCGAT 2 cut(s) 79, 353
CseI GACGC 2 cut(s) 324, 812
Csp6I GTAC 3 cut(s) 266, 403, 448
CspAI ACCGGT 2 cut(s) 134, 369
CviAII CATG 4 cut(s) 332, 790, 935, 1012
CviQI GTAC 3 cut(s) 266, 403, 448
DdeI CTNAG 1 cut(s) 246
DpnI GATC 5 cut(s) 78, 185, 294, 356, 519
DpnII GATC 5 cut(s) 76, 183, 292, 354, 517
DriI GACNNNNNGTC 1 cut(s) 916
EaeI YGGCCR 4 cut(s) 177, 342, 966, 1017
EagI CGGCCG 3 cut(s) 177, 966, 1017
Eam1104I CTCTTC 1 cut(s) 666
Eam1105I GACNNNNNGTC 1 cut(s) 916
EarI CTCTTC 1 cut(s) 666
EclXI CGGCCG 3 cut(s) 177, 966, 1017
Eco31I GGTCTC 1 cut(s) 868
Eco47III AGCGCT 1 cut(s) 687
Eco52I CGGCCG 3 cut(s) 177, 966, 1017
Eco88I CYCGRG 1 cut(s) 431
Eco91I GGTNACC 1 cut(s) 372
EcoO65I GGTNACC 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 143, 357
EcoT22I ATGCAT 1 cut(s) 636
FaeI CATG 4 cut(s) 335, 793, 938, 1015
FaiI YATR 9 cut(s) 29, 155, 188, 333, 407, 594, 791, 936, 1013
FatI CATG 4 cut(s) 331, 789, 934, 1011
FauI CCCGC 2 cut(s) 477, 886
FblI GTMKAC 3 cut(s) 210, 471, 909
Fnu4HI GCNGC 9 cut(s) 6, 457, 482, 649, 767, 960, 973, 1026, 1029
FokI GGATG 1 cut(s) 535
Fsp4HI GCNGC 9 cut(s) 6, 457, 482, 649, 767, 960, 973, 1026, 1029
GlaI GCGC 2 cut(s) 647, 687
GluI GCNGC 9 cut(s) 6, 457, 482, 649, 767, 960, 973, 1026, 1029
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 2 cut(s) 380, 805
HaeII RGCGCY 1 cut(s) 689
HaeIII GGCC 6 cut(s) 179, 344, 737, 837, 968, 1019
HgaI GACGC 2 cut(s) 324, 812
HhaI GCGC 2 cut(s) 648, 688
Hin1I GRCGYC 1 cut(s) 804
Hin1II CATG 4 cut(s) 335, 793, 938, 1015
Hin6I GCGC 2 cut(s) 646, 686
HinP1I GCGC 2 cut(s) 646, 686
HincII GTYRAC 2 cut(s) 472, 921
HindII GTYRAC 2 cut(s) 472, 921
HindIII AAGCTT 1 cut(s) 813
HinfI GANTC 6 cut(s) 45, 299, 350, 676, 757, 888
HphI GGTGA 3 cut(s) 266, 565, 752
Hpy166II GTNNAC 7 cut(s) 25, 211, 421, 472, 547, 910, 921
Hpy188I TCNGA 6 cut(s) 93, 196, 441, 508, 522, 620
Hpy188III TCNNGA 7 cut(s) 232, 296, 626, 680, 715, 820, 935
Hpy8I GTNNAC 7 cut(s) 25, 211, 421, 472, 547, 910, 921
Hpy99I CGWCG 4 cut(s) 476, 584, 806, 834
HpyCH4III ACNGT 3 cut(s) 40, 289, 1038
HpyCH4IV ACGT 3 cut(s) 474, 731, 867
HpyCH4V TGCA 8 cut(s) 164, 205, 258, 634, 751, 769, 793, 1031
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 3 cut(s) 474, 731, 867
Hsp92I GRCGYC 1 cut(s) 804
Hsp92II CATG 4 cut(s) 335, 793, 938, 1015
HspAI GCGC 2 cut(s) 646, 686
Kpn2I TCCGGA 1 cut(s) 295
KroI GCCGGC 2 cut(s) 968, 1015
KroNI GCCGGC 2 cut(s) 970, 1017
KspI CCGCGG 1 cut(s) 485
Kzo9I GATC 5 cut(s) 76, 183, 292, 354, 517
LmnI GCTCC 4 cut(s) 122, 361, 508, 786
Lsp1109I GCAGC 5 cut(s) 443, 753, 984, 1012, 1015
LweI GCATC 3 cut(s) 337, 621, 643
MaeII ACGT 3 cut(s) 474, 731, 867
MaeIII GTNAC 4 cut(s) 241, 365, 372, 475
MalI GATC 5 cut(s) 78, 185, 294, 356, 519
MboI GATC 5 cut(s) 76, 183, 292, 354, 517
MboII GAAGA 3 cut(s) 683, 730, 920
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 3 cut(s) 287, 429, 492
MlsI TGGCCA 1 cut(s) 344
MluCI AATT 1 cut(s) 994
MluNI TGGCCA 1 cut(s) 344
MlyI GAGTC 2 cut(s) 308, 670
MmeI TCCRAC 3 cut(s) 71, 486, 675
Mox20I TGGCCA 1 cut(s) 344
Mph1103I ATGCAT 1 cut(s) 636
MroI TCCGGA 1 cut(s) 295
MroNI GCCGGC 2 cut(s) 968, 1015
MscI TGGCCA 1 cut(s) 344
MseI TTAA 3 cut(s) 167, 324, 606
Msp20I TGGCCA 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 484
MspR9I CCNGG 4 cut(s) 145, 359, 601, 839
MvaI CCWGG 2 cut(s) 145, 359
MvnI CGCG 2 cut(s) 484, 648
NaeI GCCGGC 2 cut(s) 970, 1017
NciI CCSGG 2 cut(s) 601, 839
NdeII GATC 5 cut(s) 76, 183, 292, 354, 517
NgoMIV GCCGGC 2 cut(s) 968, 1015
NlaIII CATG 4 cut(s) 335, 793, 938, 1015
NlaIV GGNNCC 3 cut(s) 124, 133, 294
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 241, 365
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 793
NspV TTCGAA 1 cut(s) 707
PaeI GCATGC 1 cut(s) 793
PagI TCATGA 1 cut(s) 934
PcsI WCGNNNNNNNCGW 2 cut(s) 429, 807
PdiI GCCGGC 2 cut(s) 970, 1017
PfeI GAWTC 4 cut(s) 45, 350, 757, 888
PfoI TCCNGGA 1 cut(s) 357
PinAI ACCGGT 2 cut(s) 134, 369
PkrI GCNGC 9 cut(s) 7, 458, 483, 650, 768, 961, 974, 1027, 1030
PleI GAGTC 2 cut(s) 307, 670
PpsI GAGTC 2 cut(s) 307, 670
Psp6I CCWGG 2 cut(s) 143, 357
PspEI GGTNACC 1 cut(s) 372
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 2 cut(s) 143, 357
PspN4I GGNNCC 3 cut(s) 124, 133, 294
PspPI GGNCC 1 cut(s) 736
PstI CTGCAG 1 cut(s) 771
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 3 cut(s) 267, 404, 449
RsaNI GTAC 3 cut(s) 266, 403, 448
SacII CCGCGG 1 cut(s) 485
SalI GTCGAC 1 cut(s) 470
SaqAI TTAA 3 cut(s) 167, 324, 606
SatI GCNGC 9 cut(s) 6, 457, 482, 649, 767, 960, 973, 1026, 1029
Sau3AI GATC 5 cut(s) 76, 183, 292, 354, 517
Sau96I GGNCC 1 cut(s) 736
SchI GAGTC 2 cut(s) 308, 670
ScrFI CCNGG 4 cut(s) 145, 359, 601, 839
SduI GDGCHC 3 cut(s) 287, 429, 492
SfaNI GCATC 3 cut(s) 337, 621, 643
SfcI CTRYAG 3 cut(s) 592, 767, 954
Sfr303I CCGCGG 1 cut(s) 485
SfuI TTCGAA 1 cut(s) 707
SgrAI CRCCGGYG 1 cut(s) 386
SgrBI CCGCGG 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
SphI GCATGC 1 cut(s) 793
Sse9I AATT 1 cut(s) 994
SsiI CCGC 8 cut(s) 482, 484, 648, 796, 826, 879, 959, 1004
SspI AATATT 1 cut(s) 173
StyD4I CCNGG 4 cut(s) 143, 357, 599, 837
TaaI ACNGT 3 cut(s) 40, 289, 1038
TaiI ACGT 3 cut(s) 477, 734, 870
TaqI TCGA 8 cut(s) 79, 302, 353, 471, 567, 707, 801, 832
TasI AATT 1 cut(s) 994
TauI GCSGC 3 cut(s) 484, 651, 962
TfiI GAWTC 4 cut(s) 45, 350, 757, 888
Tru1I TTAA 3 cut(s) 167, 324, 606
Tru9I TTAA 3 cut(s) 167, 324, 606
TscAI CASTG 5 cut(s) 250, 294, 420, 946, 994
TseFI GTSAC 2 cut(s) 241, 365
TseI GCWGC 6 cut(s) 5, 456, 766, 972, 1025, 1028
Tsp45I GTSAC 2 cut(s) 241, 365
TspDTI ATGAA 1 cut(s) 684
TspRI CASTG 5 cut(s) 250, 294, 420, 946, 994
XceI RCATGY 1 cut(s) 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 1018
XmiI GTMKAC 3 cut(s) 210, 471, 909
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.