FvH4_4g17950

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008027
Physical Location & Seq
Reverse (-)
21924128 .. 21927673
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17950.t4

Sequence Viewer

Length: 1203 bp
ATGTCAAACTTAAGTTGCAGTCCTTCTACGCCCGAGTCTGATTCTTACTTGATGAAATCTATGAGTCCTACATCGAGAGGTGAAGGTTTTCTCAATTCCATGGAAGTACAAGTGGCAGGTGGAGCTGCTGGTGAAGACAGGGTCCAAGCGGTTTGTTCTGAAGAAAATGGGTGGCTGTATTGTGCAATATATGATGGCTTTAATGGAAGAGACGCAGCTGATTTCCTGGCTGGGACACTGTATGAAACCATTGTTTTTTACTTCAATTCATTGGATTGGGAATCAAATCAGGAATCCATCAATGCTTCTAAAGGGATAAATATTGATGGGTCTCTTCCATTTGTTAAAGATGATAGTGATATTGAAAATATCTCCAAGGATGCTTCATCTACTGAAATAGAAATGTCAACTGATACATTCAGGAATGGGGTCCTTGATAGCCTACAACAAGCTCTTAGTCAGGCCGAGAATGATTTTCTATACATGGTTGAGCAGGAAATGGAGGATCGCCCTGATTTGGTCTCTGTTGGGTCTTGTGTTTTGGTTGTGCTTCTTCATGGGAATGATTTGTACACACTTAATTTAGGTGACAGCCGGGCTGTGTTGGCAACACATGGTGATGGCGATTTCATGAATGGGAGTGAGAGACTGAAAGCTATTCAGCTCACAGGAAGTCATACAGTTGATAACGAAGTTGAAAGAACGCGAGTTTTGTGTGAGCATCCTGATGACCCCATGTCCATTGTGGCTGGAAAAGTAAAAGGGAAATTGAAAGTCACTCGAGCTTTTGGAGTTGGTTATTTGAAAAAGAAAAAACTGAATGATGCATTGATGGGGATTCTTCGAGTTCGTAATCTAATAAGCCCACCGTATGTTTCTACTCAACCATCAGTGGATGTGCGTAGAATATCAAAGTCGGATCGCTTTGTTATAGTTGCAAGTGATGGTTTGTTCGACTTCTTTACCAATGATGAGGCGGTAAAGCTTGTCCATTCCTATATCTCGAGTAACCCCTCGGGTGATCCATCCAAGTTTTTGCTGGAGCAGCTTGTAGTGAGAGCAGCCAACTCTGCAGGATTCAGTATGGAAGAGTTGATGAATATACCAGCGGGGAGGAGGAGGAAGTATCATGATGATGTAACTGTAATTGTGGTTATTCTTGGTACAAATCACCGTACCACAAAGGCATCAACTTGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

401

Amino Acids

43.81

Weight (kDa)

4.84

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 246 - 441 7.7e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 107
Acc36I ACCTGC 1 cut(s) 107
AccII CGCG 1 cut(s) 706
AciI CCGC 3 cut(s) 149, 977, 1109
AclWI GGATC 3 cut(s) 513, 927, 1016
AcuI CTGAAG 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 617
AfaI GTAC 4 cut(s) 108, 572, 1165, 1177
AfiI CCNNNNNNNGG 1 cut(s) 517
AflII CTTAAG 1 cut(s) 10
AgsI TTSAA 5 cut(s) 265, 365, 698, 772, 805
AhdI GACNNNNNGTC 1 cut(s) 736
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 8 cut(s) 125, 218, 452, 656, 664, 785, 985, 1048
AluI AGCT 8 cut(s) 125, 218, 452, 656, 664, 785, 985, 1048
Alw26I GTCTC 4 cut(s) 204, 336, 526, 640
AlwI GGATC 3 cut(s) 513, 927, 1016
Ama87I CYCGRG 4 cut(s) 32, 780, 1003, 1015
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 4 cut(s) 125, 215, 1045, 1061
Asp700I GAANNNNTTC 1 cut(s) 87
AspS9I GGNCC 2 cut(s) 142, 430
AsuC2I CCSGG 1 cut(s) 596
AsuHPI GGTGA 6 cut(s) 92, 143, 599, 629, 1031, 1163
AvaI CYCGRG 4 cut(s) 32, 780, 1003, 1015
AvaII GGWCC 2 cut(s) 142, 430
BbsI GAAGAC 1 cut(s) 141
BbvI GCAGC 4 cut(s) 112, 227, 1057, 1073
BccI CCATC 8 cut(s) 188, 305, 320, 614, 826, 895, 938, 1033
BciT130I CCWGG 1 cut(s) 227
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 4 cut(s) 204, 336, 526, 640
BfmI CTRYAG 1 cut(s) 1071
BfrI CTTAAG 1 cut(s) 10
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 4 cut(s) 126, 216, 1046, 1062
BlsI GCNGC 4 cut(s) 127, 217, 1047, 1063
Bme1390I CCNGG 2 cut(s) 227, 596
Bme18I GGWCC 2 cut(s) 142, 430
BmeRI GACNNNNNGTC 1 cut(s) 736
BmeT110I CYCGRG 4 cut(s) 32, 780, 1003, 1015
BmgT120I GGNCC 2 cut(s) 142, 430
BmiI GGNNCC 2 cut(s) 143, 431
BmrFI CCNGG 2 cut(s) 227, 596
BmsI GCATC 4 cut(s) 370, 730, 814, 1196
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 1061
BpuMI CCSGG 1 cut(s) 596
BsaI GGTCTC 2 cut(s) 336, 526
BsaJI CCNNGG 3 cut(s) 99, 375, 1014
Bsc4I CCNNNNNNNGG 1 cut(s) 517
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 3 cut(s) 99, 375, 1014
BseGI GGATG 4 cut(s) 385, 721, 901, 1025
BseLI CCNNNNNNNGG 1 cut(s) 517
BseRI GAGGAG 2 cut(s) 1129, 1132
BseXI GCAGC 4 cut(s) 112, 227, 1057, 1073
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 706
BshFI GGCC 1 cut(s) 464
BsiHKCI CYCGRG 4 cut(s) 32, 780, 1003, 1015
BsiSI CCGG 1 cut(s) 595
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 517
BsmAI GTCTC 4 cut(s) 204, 336, 526, 640
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 1 cut(s) 464
Bso31I GGTCTC 2 cut(s) 336, 526
BsoBI CYCGRG 4 cut(s) 32, 780, 1003, 1015
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 3 cut(s) 505, 919, 1021
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 3 cut(s) 149, 977, 1109
BspANI GGCC 1 cut(s) 464
BspFNI CGCG 1 cut(s) 706
BspHI TCATGA 2 cut(s) 630, 1129
BspLI GGNNCC 2 cut(s) 143, 431
BspMAI CTGCAG 1 cut(s) 1075
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 3 cut(s) 513, 927, 1016
BspTI CTTAAG 1 cut(s) 10
BspTNI GGTCTC 2 cut(s) 336, 526
BsrGI TGTACA 1 cut(s) 570
BssECI CCNNGG 3 cut(s) 99, 375, 1014
BssMI GATC 3 cut(s) 505, 919, 1021
BssT1I CCWWGG 2 cut(s) 99, 375
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 5 cut(s) 240, 682, 870, 1144, 1175
Bst6I CTCTTC 3 cut(s) 202, 339, 1083
BstAFI CTTAAG 1 cut(s) 10
BstAUI TGTACA 1 cut(s) 570
BstDEI CTNAG 1 cut(s) 455
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 4 cut(s) 385, 721, 901, 1025
BstFNI CGCG 1 cut(s) 706
BstKTI GATC 3 cut(s) 508, 922, 1024
BstMAI GTCTC 4 cut(s) 204, 336, 526, 640
BstMBI GATC 3 cut(s) 505, 919, 1021
BstMWI GCNNNNNNNGC 4 cut(s) 122, 605, 1045, 1070
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 225, 594
BstSFI CTRYAG 1 cut(s) 1071
BstUI CGCG 1 cut(s) 706
BstV1I GCAGC 4 cut(s) 112, 227, 1057, 1073
BstV2I GAAGAC 1 cut(s) 141
BsuRI GGCC 1 cut(s) 464
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 4 cut(s) 385, 721, 901, 1025
BtsIMutI CAGTG 2 cut(s) 236, 897
BveI ACCTGC 1 cut(s) 107
CciI TCATGA 2 cut(s) 630, 1129
Cfr13I GGNCC 2 cut(s) 142, 430
CseI GACGC 1 cut(s) 221
Csp6I GTAC 4 cut(s) 107, 571, 1164, 1176
CviAII CATG 7 cut(s) 100, 484, 557, 614, 631, 736, 1130
CviQI GTAC 4 cut(s) 107, 571, 1164, 1176
DdeI CTNAG 1 cut(s) 455
DpnI GATC 3 cut(s) 507, 921, 1023
DpnII GATC 3 cut(s) 505, 919, 1021
DraIII CACNNNGTG 1 cut(s) 617
DriI GACNNNNNGTC 1 cut(s) 736
Eam1104I CTCTTC 3 cut(s) 202, 339, 1083
Eam1105I GACNNNNNGTC 1 cut(s) 736
EarI CTCTTC 3 cut(s) 202, 339, 1083
Eco130I CCWWGG 2 cut(s) 99, 375
Eco31I GGTCTC 2 cut(s) 336, 526
Eco47I GGWCC 2 cut(s) 142, 430
Eco57I CTGAAG 1 cut(s) 180
Eco88I CYCGRG 4 cut(s) 32, 780, 1003, 1015
EcoO109I RGGNCCY 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 2 cut(s) 99, 375
EcoT22I ATGCAT 1 cut(s) 829
ErhI CCWWGG 2 cut(s) 99, 375
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 7 cut(s) 103, 487, 560, 617, 634, 739, 1133
FaqI GGGAC 1 cut(s) 247
FatI CATG 7 cut(s) 99, 483, 556, 613, 630, 735, 1129
FauI CCCGC 1 cut(s) 1102
Fnu4HI GCNGC 4 cut(s) 126, 216, 1046, 1062
FokI GGATG 4 cut(s) 392, 708, 908, 1012
Fsp4HI GCNGC 4 cut(s) 126, 216, 1046, 1062
GluI GCNGC 4 cut(s) 126, 216, 1046, 1062
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 1061
HaeIII GGCC 1 cut(s) 464
HapII CCGG 1 cut(s) 595
HgaI GACGC 1 cut(s) 221
Hin1II CATG 7 cut(s) 103, 487, 560, 617, 634, 739, 1133
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 7 cut(s) 35, 41, 64, 281, 293, 838, 1077
HpaII CCGG 1 cut(s) 595
HphI GGTGA 6 cut(s) 92, 143, 599, 629, 1031, 1163
Hpy166II GTNNAC 2 cut(s) 408, 573
Hpy188I TCNGA 3 cut(s) 40, 160, 919
Hpy188III TCNNGA 7 cut(s) 75, 290, 421, 631, 725, 1003, 1130
Hpy8I GTNNAC 2 cut(s) 408, 573
HpyAV CCTTC 2 cut(s) 33, 77
HpyCH4III ACNGT 5 cut(s) 240, 682, 870, 1144, 1175
HpyCH4V TGCA 5 cut(s) 18, 185, 827, 938, 1073
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 605, 1045, 1070
HpyF3I CTNAG 1 cut(s) 455
Hsp92II CATG 7 cut(s) 103, 487, 560, 617, 634, 739, 1133
Kzo9I GATC 3 cut(s) 505, 919, 1021
LmnI GCTCC 2 cut(s) 122, 1042
Lsp1109I GCAGC 4 cut(s) 112, 227, 1057, 1073
LweI GCATC 4 cut(s) 370, 730, 814, 1196
MaeIII GTNAC 4 cut(s) 587, 775, 1007, 1138
MalI GATC 3 cut(s) 507, 921, 1023
MboI GATC 3 cut(s) 505, 919, 1021
MboII GAAGA 7 cut(s) 146, 173, 219, 326, 545, 833, 1100
MluCI AATT 5 cut(s) 94, 265, 580, 767, 1146
MlyI GAGTC 2 cut(s) 44, 73
MmeI TCCRAC 1 cut(s) 897
MnlI CCTC 7 cut(s) 71, 496, 967, 1024, 1107, 1110, 1113
Mph1103I ATGCAT 1 cut(s) 829
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 5 cut(s) 11, 201, 345, 579, 1201
MslI CAYNNNNRTG 4 cut(s) 561, 618, 726, 1134
MspA1I CMGCKG 2 cut(s) 218, 1109
MspCI CTTAAG 1 cut(s) 10
MspI CCGG 1 cut(s) 595
MspR9I CCNGG 2 cut(s) 227, 596
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 706
MwoI GCNNNNNNNGC 4 cut(s) 122, 605, 1045, 1070
NciI CCSGG 1 cut(s) 596
NcoI CCATGG 1 cut(s) 99
NdeII GATC 3 cut(s) 505, 919, 1021
NlaIII CATG 7 cut(s) 103, 487, 560, 617, 634, 739, 1133
NlaIV GGNNCC 2 cut(s) 143, 431
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 2 cut(s) 587, 775
NsiI ATGCAT 1 cut(s) 829
PaeR7I CTCGAG 2 cut(s) 780, 1003
PagI TCATGA 2 cut(s) 630, 1129
PaqCI CACCTGC 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 5 cut(s) 41, 281, 293, 838, 1077
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 4 cut(s) 127, 217, 1047, 1063
PleI GAGTC 2 cut(s) 43, 72
PpsI GAGTC 2 cut(s) 43, 72
PpuMI RGGWCCY 1 cut(s) 430
Psp5II RGGWCCY 1 cut(s) 430
Psp6I CCWGG 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 143, 431
PspPI GGNCC 2 cut(s) 142, 430
PspPPI RGGWCCY 1 cut(s) 430
PspXI VCTCGAGB 1 cut(s) 780
PstI CTGCAG 1 cut(s) 1075
PsyI GACNNNGTC 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 4 cut(s) 108, 572, 1165, 1177
RsaNI GTAC 4 cut(s) 107, 571, 1164, 1176
RseI CAYNNNNRTG 4 cut(s) 561, 618, 726, 1134
SaqAI TTAA 5 cut(s) 11, 201, 345, 579, 1201
SatI GCNGC 4 cut(s) 126, 216, 1046, 1062
Sau3AI GATC 3 cut(s) 505, 919, 1021
Sau96I GGNCC 2 cut(s) 142, 430
SchI GAGTC 2 cut(s) 44, 73
ScrFI CCNGG 2 cut(s) 227, 596
SfaNI GCATC 4 cut(s) 370, 730, 814, 1196
SfcI CTRYAG 1 cut(s) 1071
Sfr274I CTCGAG 2 cut(s) 780, 1003
SinI GGWCC 2 cut(s) 142, 430
SlaI CTCGAG 2 cut(s) 780, 1003
SmiMI CAYNNNNRTG 4 cut(s) 561, 618, 726, 1134
SmlI CTYRAG 3 cut(s) 10, 780, 1003
SmoI CTYRAG 3 cut(s) 10, 780, 1003
Sse9I AATT 5 cut(s) 94, 265, 580, 767, 1146
SsiI CCGC 3 cut(s) 149, 977, 1109
SspI AATATT 1 cut(s) 322
StyD4I CCNGG 2 cut(s) 225, 594
StyI CCWWGG 2 cut(s) 99, 375
TaaI ACNGT 5 cut(s) 240, 682, 870, 1144, 1175
TaqI TCGA 5 cut(s) 74, 781, 844, 954, 1004
TasI AATT 5 cut(s) 94, 265, 580, 767, 1146
TatI WGTACW 2 cut(s) 106, 570
TfiI GAWTC 5 cut(s) 41, 281, 293, 838, 1077
Tru1I TTAA 5 cut(s) 11, 201, 345, 579, 1201
Tru9I TTAA 5 cut(s) 11, 201, 345, 579, 1201
TscAI CASTG 2 cut(s) 243, 897
TseFI GTSAC 2 cut(s) 587, 775
TseI GCWGC 4 cut(s) 125, 215, 1045, 1061
Tsp45I GTSAC 2 cut(s) 587, 775
TspDTI ATGAA 8 cut(s) 68, 258, 258, 375, 545, 619, 647, 1112
TspRI CASTG 2 cut(s) 243, 897
Tth111I GACNNNGTC 1 cut(s) 140
Vha464I CTTAAG 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 142, 430
XcmI CCANNNNNNNNNTGG 2 cut(s) 742, 1036
XhoI CTCGAG 2 cut(s) 780, 1003
XmnI GAANNNNTTC 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.