Rh4AG237000

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008027
Physical Location & Seq
Reverse (-)
54179271 .. 54184422
5152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG237000.1

Sequence Viewer

Length: 1509 bp
ATGCTGGGAGAAACTATGATTAAACCTGAAGGAGAACTTAAAGTAAGCTTTGGTTATCAATGCAGTAGTGACAGAGTTGATTCTTGTGAGGCTTCCAGTGGATATGAAATCCTGCCTGACCTCCGAAGGAACAGTAGTTTCTCTTGCTTGTCTGGAGCTGCGTTAAGTGCCAATGCCACTCTGGCAAACACTAATATCTGCAATGGTTTGATAGGGGAAGAAATACTTCCTAGTTGGGACTCCCCTAATTCATTTCGTAAGGTACCCTCTTCGCCAAGGTTGGATATATTATCATCTTCTCTTCAGAGTAGTATGTCAAACTTAAGTTGCAGTCCATCTACACCCGAGTCTGATTCTTACTTGATGAAATCCATGAGTCCCACTTCCAGAGGTGAAGGTTTTCTCAATTCCATGGAAGTACAAGTGGCAGGTGGAGCTGCTGGTGAAGACAGGGTCCAAGCGGTTTGTTCTGAAGAAAATGGGTGGCTGTATTGTGCAATATACGATGGCTTTAATGGAAGAGATGCAGCTGATTTTCTGGCTGGGACACTATATGAAACCATTGTATTTTACTTTAATTCATTAGACTGGGAATCAAATCAGGAATCCATCAATGCTTCTAAAGGGCTAAATATTGATGGGTCTCGTCCATTTATTGAAGATGATAGTAATATTGAAAATATCTCCAAGGATGCTTCATCTACTGACGTAGAAATGTCAACTGATTCATTCAGGCATGGGGTACTTGATAGCCTACAACGAGCTCTTAGTCAGGCCGAGAATGATTTTCTATACATGGTTGAGCAGGAAATGGAGGATCGTCCTGATTTGGTTTCTGTTGGGTCTTGTGTTTTAGTTGTGCTTCTTCATGGGAATGATTTGTATACACTTAATTTAGGTGACAGCCGGGCTGTGTTGGCAACACTTGGTGATGGTGATTTCATGAATGGGAGTGAGAGACTGAAAGCTATCCAGCTCACTGAAAGTCATACGGTTGATGATGAAGTTGAAAGAATGCGAGTTTTGTGCGATCATCCTGATGACCCCATGTCCATTGTGGCTGGAAAAGTAAAAGGGAAATTGAAGGTCACTCGAGCTTTTGGAGTTGGTTACTTGAAAAAGAAAAAACTGAATGACGCATTGATGGGGATTCTTCAAGTTCGTAATCTAATAAGCCCACCATATGTTTCTACTCAACCATCATTGAGTGTGCGTAGAATATCAAAGTCGGATCGATTTGTCATAGTTGCAAGTGATGGTTTGTTTGACTTTTTTACCAATGACGAGGCAGTAAAGCTTGTCCATTCCTATATCTTGAGCAACCCCTCTGGTGATCCATCCAAGTTTCTGCTGGAGCAGCTTGTAGTGAGAGCAGCCAACTGTGCAGGTAGACTTTTTAAGATGTCATTATGTAGATTTGTTGTCATTTCATTCAAATCACCAACAGGTGGCTCCTTGTATCCTTTTGGGTGTTTATTACACAAGAAGCATTTACAAGTAAGACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

502

Amino Acids

54.91

Weight (kDa)

4.92

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 243 - 442 3.4e-31 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 419
Acc36I ACCTGC 2 cut(s) 419, 1376
Acc65I GGTACC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 262
AccB7I CCANNNNNTGG 1 cut(s) 1448
AccI GTMKAC 2 cut(s) 884, 1390
AciI CCGC 1 cut(s) 461
AclWI GGATC 3 cut(s) 825, 1239, 1328
AcuI CTGAAG 3 cut(s) 48, 287, 492
AdeI CACNNNGTG 1 cut(s) 929
AfaI GTAC 3 cut(s) 264, 420, 744
AfiI CCNNNNNNNGG 1 cut(s) 1448
AflII CTTAAG 1 cut(s) 322
AgsI TTSAA 7 cut(s) 659, 677, 1010, 1084, 1117, 1157, 1435
AhdI GACNNNNNGTC 1 cut(s) 1048
Alw21I GWGCWC 1 cut(s) 766
Alw26I GTCTC 2 cut(s) 648, 952
AlwI GGATC 3 cut(s) 825, 1239, 1328
Ama87I CYCGRG 2 cut(s) 344, 1092
AoxI GGCC 1 cut(s) 774
ApeKI GCWGC 5 cut(s) 158, 437, 527, 1357, 1373
Asp700I GAANNNNTTC 2 cut(s) 225, 399
Asp718I GGTACC 1 cut(s) 262
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 1 cut(s) 908
AsuHPI GGTGA 7 cut(s) 404, 455, 911, 941, 947, 1343, 1431
AvaI CYCGRG 2 cut(s) 344, 1092
AvaII GGWCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 766
BbvI GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
BccI CCATC 9 cut(s) 343, 500, 617, 632, 926, 1138, 1207, 1250, 1345
BcgI CGANNNNNNTGC 2 cut(s) 1008, 1042
BciVI GTATCC 1 cut(s) 1470
BcnI CCSGG 1 cut(s) 908
BcoDI GTCTC 2 cut(s) 648, 952
BfaI CTAG 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 322
BfuAI ACCTGC 2 cut(s) 419, 1376
BfuI GTATCC 1 cut(s) 1470
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
BlsI GCNGC 5 cut(s) 160, 439, 529, 1359, 1375
Bme1390I CCNGG 1 cut(s) 908
Bme18I GGWCC 1 cut(s) 454
BmeRI GACNNNNNGTC 1 cut(s) 1048
BmeT110I CYCGRG 2 cut(s) 344, 1092
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 3 cut(s) 264, 455, 1453
BmrFI CCNGG 1 cut(s) 908
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 2 cut(s) 514, 682
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 453
BpmI CTGGAG 2 cut(s) 174, 1373
BpuEI CTTGAG 1 cut(s) 1336
BpuMI CCSGG 1 cut(s) 908
Bsa29I ATCGAT 1 cut(s) 1234
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 275, 411, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 1448
Bse1I ACTGG 2 cut(s) 96, 593
Bse3DI GCAATG 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 1234
BseDI CCNNGG 3 cut(s) 275, 411, 687
BseGI GGATG 3 cut(s) 697, 1033, 1337
BseLI CCNNNNNNNGG 1 cut(s) 1448
BseMI GCAATG 1 cut(s) 208
BseNI ACTGG 2 cut(s) 96, 593
BseXI GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
BseYI CCCAGC 2 cut(s) 4, 542
BsgI GTGCAG 1 cut(s) 1404
BshFI GGCC 1 cut(s) 776
BshNI GGYRCC 1 cut(s) 262
BshVI ATCGAT 1 cut(s) 1234
BsiHKAI GWGCWC 1 cut(s) 766
BsiHKCI CYCGRG 2 cut(s) 344, 1092
BsiSI CCGG 1 cut(s) 907
BslFI GGGAC 3 cut(s) 251, 363, 559
BslI CCNNNNNNNGG 1 cut(s) 1448
BsmAI GTCTC 2 cut(s) 648, 952
BsmFI GGGAC 3 cut(s) 251, 363, 559
BsmI GAATGC 1 cut(s) 1020
BsnI GGCC 1 cut(s) 776
Bso31I GGTCTC 1 cut(s) 648
BsoBI CYCGRG 2 cut(s) 344, 1092
Bsp1286I GDGCHC 1 cut(s) 766
Bsp143I GATC 4 cut(s) 817, 1030, 1231, 1333
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 1 cut(s) 461
BspANI GGCC 1 cut(s) 776
BspDI ATCGAT 1 cut(s) 1234
BspHI TCATGA 1 cut(s) 942
BspLI GGNNCC 3 cut(s) 264, 455, 1453
BspMI ACCTGC 2 cut(s) 419, 1376
BspPI GGATC 3 cut(s) 825, 1239, 1328
BspT107I GGYRCC 1 cut(s) 262
BspTI CTTAAG 1 cut(s) 322
BspTNI GGTCTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 2 cut(s) 96, 593
BssECI CCNNGG 3 cut(s) 275, 411, 687
BssMI GATC 4 cut(s) 817, 1030, 1231, 1333
BssNAI GTATAC 1 cut(s) 885
BssT1I CCWWGG 3 cut(s) 275, 411, 687
Bst1107I GTATAC 1 cut(s) 885
Bst4CI ACNGT 3 cut(s) 134, 994, 1382
Bst6I CTCTTC 3 cut(s) 274, 306, 514
BstAFI CTTAAG 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 767
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 3 cut(s) 697, 1033, 1337
BstKTI GATC 4 cut(s) 820, 1033, 1234, 1336
BstMAI GTCTC 2 cut(s) 648, 952
BstMBI GATC 4 cut(s) 817, 1030, 1231, 1333
BstMWI GCNNNNNNNGC 6 cut(s) 167, 182, 434, 917, 1357, 1382
BstSCI CCNGG 1 cut(s) 906
BstV1I GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
BstV2I GAAGAC 1 cut(s) 453
BstZ17I GTATAC 1 cut(s) 885
Bsu15I ATCGAT 1 cut(s) 1234
BsuI GTATCC 1 cut(s) 1470
BsuRI GGCC 1 cut(s) 776
BsuTUI ATCGAT 1 cut(s) 1234
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 3 cut(s) 697, 1033, 1337
BtsIMutI CAGTG 2 cut(s) 103, 978
BveI ACCTGC 2 cut(s) 419, 1376
CciI TCATGA 1 cut(s) 942
Cfr13I GGNCC 1 cut(s) 454
ClaI ATCGAT 1 cut(s) 1234
CseI GACGC 1 cut(s) 1145
Csp6I GTAC 3 cut(s) 263, 419, 743
CviAII CATG 7 cut(s) 373, 412, 737, 796, 869, 943, 1048
CviQI GTAC 3 cut(s) 263, 419, 743
DdeI CTNAG 1 cut(s) 767
DpnI GATC 4 cut(s) 819, 1032, 1233, 1335
DpnII GATC 4 cut(s) 817, 1030, 1231, 1333
DraIII CACNNNGTG 1 cut(s) 929
DriI GACNNNNNGTC 1 cut(s) 1048
Eam1104I CTCTTC 3 cut(s) 274, 306, 514
Eam1105I GACNNNNNGTC 1 cut(s) 1048
EarI CTCTTC 3 cut(s) 274, 306, 514
Ecl136II GAGCTC 1 cut(s) 764
Eco130I CCWWGG 3 cut(s) 275, 411, 687
Eco24I GRGCYC 1 cut(s) 766
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 764
Eco57I CTGAAG 3 cut(s) 48, 287, 492
Eco88I CYCGRG 2 cut(s) 344, 1092
EcoICRI GAGCTC 1 cut(s) 764
EcoT14I CCWWGG 3 cut(s) 275, 411, 687
EcoT38I GRGCYC 1 cut(s) 766
ErhI CCWWGG 3 cut(s) 275, 411, 687
FaeI CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
FalI AAGNNNNNCTT 5 cut(s) 21, 53, 210, 242, 1488
FaqI GGGAC 3 cut(s) 251, 363, 559
FatI CATG 7 cut(s) 372, 411, 736, 795, 868, 942, 1047
FauNDI CATATG 1 cut(s) 1183
FblI GTMKAC 2 cut(s) 884, 1390
Fnu4HI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
FokI GGATG 3 cut(s) 704, 1020, 1324
FriOI GRGCYC 1 cut(s) 766
Fsp4HI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
FspBI CTAG 1 cut(s) 231
GluI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
GsaI CCCAGC 2 cut(s) 8, 546
GsuI CTGGAG 2 cut(s) 174, 1373
HaeIII GGCC 1 cut(s) 776
HapII CCGG 1 cut(s) 907
HgaI GACGC 1 cut(s) 1145
Hin1II CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HindIII AAGCTT 2 cut(s) 46, 1295
HinfI GANTC 9 cut(s) 80, 239, 347, 353, 376, 593, 605, 725, 1150
HpaII CCGG 1 cut(s) 907
HphI GGTGA 7 cut(s) 404, 455, 911, 941, 947, 1343, 1431
Hpy166II GTNNAC 3 cut(s) 720, 885, 1391
Hpy188I TCNGA 5 cut(s) 125, 306, 352, 472, 1231
Hpy188III TCNNGA 7 cut(s) 153, 387, 602, 824, 943, 1037, 1315
Hpy8I GTNNAC 3 cut(s) 720, 885, 1391
HpyAV CCTTC 4 cut(s) 23, 120, 389, 1078
HpyCH4III ACNGT 3 cut(s) 134, 994, 1382
HpyCH4IV ACGT 1 cut(s) 708
HpyCH4V TGCA 7 cut(s) 63, 201, 330, 497, 527, 1250, 1385
HpyF10VI GCNNNNNNNGC 6 cut(s) 167, 182, 434, 917, 1357, 1382
HpyF3I CTNAG 1 cut(s) 767
HpySE526I ACGT 1 cut(s) 708
Hsp92II CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
KpnI GGTACC 1 cut(s) 266
Kzo9I GATC 4 cut(s) 817, 1030, 1231, 1333
LmnI GCTCC 4 cut(s) 155, 434, 1354, 1457
Lsp1109I GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
LweI GCATC 2 cut(s) 514, 682
MaeI CTAG 1 cut(s) 231
MaeII ACGT 1 cut(s) 708
MaeIII GTNAC 4 cut(s) 68, 899, 1087, 1109
MalI GATC 4 cut(s) 819, 1032, 1233, 1335
MboI GATC 4 cut(s) 817, 1030, 1231, 1333
MhlI GDGCHC 1 cut(s) 766
MluCI AATT 5 cut(s) 247, 406, 577, 892, 1079
MlyI GAGTC 3 cut(s) 233, 356, 385
MmeI TCCRAC 2 cut(s) 261, 1209
MnlI CCTC 7 cut(s) 82, 131, 277, 383, 808, 1279, 1336
MroXI GAANNNNTTC 2 cut(s) 225, 399
MseI TTAA 9 cut(s) 21, 39, 164, 323, 513, 576, 891, 1398, 1507
MslI CAYNNNNRTG 2 cut(s) 873, 1038
MspA1I CMGCKG 1 cut(s) 530
MspCI CTTAAG 1 cut(s) 322
MspI CCGG 1 cut(s) 907
MspR9I CCNGG 1 cut(s) 908
Mva1269I GAATGC 1 cut(s) 1020
MwoI GCNNNNNNNGC 6 cut(s) 167, 182, 434, 917, 1357, 1382
NciI CCSGG 1 cut(s) 908
NcoI CCATGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 1183
NdeII GATC 4 cut(s) 817, 1030, 1231, 1333
NlaIII CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
NlaIV GGNNCC 3 cut(s) 264, 455, 1453
NmeAIII GCCGAG 1 cut(s) 802
NmuCI GTSAC 3 cut(s) 68, 899, 1087
PaeR7I CTCGAG 1 cut(s) 1092
PagI TCATGA 1 cut(s) 942
PaqCI CACCTGC 1 cut(s) 419
PctI GAATGC 1 cut(s) 1020
PdmI GAANNNNTTC 2 cut(s) 225, 399
PfeI GAWTC 6 cut(s) 80, 353, 593, 605, 725, 1150
PflFI GACNNNGTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 1448
PkrI GCNGC 5 cut(s) 160, 439, 529, 1359, 1375
PleI GAGTC 3 cut(s) 233, 355, 384
PpsI GAGTC 3 cut(s) 233, 355, 384
Psp124BI GAGCTC 1 cut(s) 766
PspFI CCCAGC 2 cut(s) 4, 542
PspN4I GGNNCC 3 cut(s) 264, 455, 1453
PspPI GGNCC 1 cut(s) 454
PspXI VCTCGAGB 1 cut(s) 1092
PsyI GACNNNGTC 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 264, 420, 744
RsaNI GTAC 3 cut(s) 263, 419, 743
RseI CAYNNNNRTG 2 cut(s) 873, 1038
SacI GAGCTC 1 cut(s) 766
SaqAI TTAA 9 cut(s) 21, 39, 164, 323, 513, 576, 891, 1398, 1507
SatI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
Sau3AI GATC 4 cut(s) 817, 1030, 1231, 1333
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 3 cut(s) 233, 356, 385
ScrFI CCNGG 1 cut(s) 908
SduI GDGCHC 1 cut(s) 766
SfaNI GCATC 2 cut(s) 514, 682
Sfr274I CTCGAG 1 cut(s) 1092
SinI GGWCC 1 cut(s) 454
SlaI CTCGAG 1 cut(s) 1092
SmiMI CAYNNNNRTG 2 cut(s) 873, 1038
SmlI CTYRAG 3 cut(s) 322, 1092, 1315
SmoI CTYRAG 3 cut(s) 322, 1092, 1315
Sse9I AATT 5 cut(s) 247, 406, 577, 892, 1079
SsiI CCGC 1 cut(s) 461
SspI AATATT 2 cut(s) 634, 673
SspMI CTAG 1 cut(s) 231
SstI GAGCTC 1 cut(s) 766
StyD4I CCNGG 1 cut(s) 906
StyI CCWWGG 3 cut(s) 275, 411, 687
TaaI ACNGT 3 cut(s) 134, 994, 1382
TaiI ACGT 1 cut(s) 711
TaqI TCGA 2 cut(s) 1093, 1234
TasI AATT 5 cut(s) 247, 406, 577, 892, 1079
TatI WGTACW 1 cut(s) 418
TfiI GAWTC 6 cut(s) 80, 353, 593, 605, 725, 1150
Tru1I TTAA 9 cut(s) 21, 39, 164, 323, 513, 576, 891, 1398, 1507
Tru9I TTAA 9 cut(s) 21, 39, 164, 323, 513, 576, 891, 1398, 1507
TscAI CASTG 2 cut(s) 103, 985
TseFI GTSAC 3 cut(s) 68, 899, 1087
TseI GCWGC 5 cut(s) 158, 437, 527, 1357, 1373
Tsp45I GTSAC 3 cut(s) 68, 899, 1087
TspRI CASTG 2 cut(s) 103, 985
Tth111I GACNNNGTC 1 cut(s) 452
Van91I CCANNNNNTGG 1 cut(s) 1448
Vha464I CTTAAG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 454
XcmI CCANNNNNNNNNTGG 3 cut(s) 178, 1054, 1348
XhoI CTCGAG 1 cut(s) 1092
XmiI GTMKAC 2 cut(s) 884, 1390
XmnI GAANNNNTTC 2 cut(s) 225, 399
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.