MD16G1170400.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008027
Physical Location & Seq
Forward (+)
14174567 .. 14178467
3901 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1170400.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGCCCTTCATCATGCTAGGAGAAACTATGGTTAAACCTGAAGGAGAGCTAAAAGTAAGCTTTGGCTATCAATGCAATAGCAACAGAGGCAGTTTTTGTGAGGATTCCGGTGAATATGAAACCCTGCCTGAATTCCGAAGGAACAGTAGTTTCTCTTGCTTGTCTGGTGCTGCCTTAAGTGCAAATGCCACTCTGGCAAACACAAATATCTGCAATGGTTTGATAGGGGCAGAAATACTTCCTAGTTGGGACTCTCCTAATTCATTTCGTAGAGTTCCCTCTTCACCGTCTATGTCAAGGTTGGATATATTATCATCTTCTTTTCAGAGTAGTATATCGAACTTAAGTTGCAGTCCATCTACGCCTGATTCTGATTCTTACTTAATGAAATCCACGAGTCCTTCCAGAAATGATAGTTTTCTTAGTGGCATGGAAGTGCAAGTGGCAGGTGGACCTGCTGGTGAAGACAGGGTCCAAGCTGTTTGTTCTGAAGAAAATGGGTGGCTCTTTTGTGCAATTTATGACGGTTTCAATGGAAGAGACGCAGCTGATTTTCTGGCTGGGACACTGTATGAAACCATTGTATTTCACTTTAATTCATTAGACTGGGAATCAAGTCAGGAATCCAACAAAGCTTCTAATGATCTAGATATAGATGAGTCCCTTCCATACATAGGAGATGATACTACTATTAGCGGGGAAAGATTTTCATCCAGAATTTATGCACACAAGGATTCAAGTTTTGACAATACCTCTAAGAATGCTCCATGTGCTAAAGTAAAAACATTAACTGATTCGTTTAGGCATGGGGTGCTTGATAGCCTCCAACGAGCTCTTAGTCAGGCTGAGAATGACTTTCTATGCATGGTTGACCAGGAAATGGTGGATCGCCCAGATTTAGTTTCTGTTGGGTGTTGTGTTTTAGTTGCACTTCTACATGGGAAGGATTTGTATACACTTAATTTAGGTGACAGCCGAGCTGTATTGGCAACATATGGTGATGGTTATTTAATGAGTGGGAGTGATAGGTTAAAAGCTATCCAGCTCACTGATAGTCATACAGTTGATGACGAAAGTGAGAGAATGAGAGTTTTGTGTGAACATCCTGACGATCCCATGACCGTCGTGGCTGGAAAAGTAAAGGGGAAATTGAAGGTCACTCGAGCTTTTGGAGTTGGTTACTTGAAAAAGAAAAAACTGAATGATGCATTGATGGGAATTCTTCAAGTTCGTAACCTCATAAGCCCACCATACGTTTCCACTCAACCAGCGTTGAATGTGCACAGAATATCAAAATCAGATTGCTTTGTAGTACTTGGTAGTGATGGCTTGTTTGACTTTTTTACGAATGACGAGGCAGTAAAGCTTGTCCATTCCTATATCTTGAGCAACCCCTCCGGCGACCCTGCTAAGTTTCTGCTCGAGCAGCTTGTAGTGAGAGCAGCCAATTCTGCAGGTCTCAGCATGGAAGAACTCATGAACATTCCGGCAGGAAGGCGGAGGAAGTATCACGATGATGTAACAGTAATTGTGATTATGCTGGGTACAAATCAGCGCACGACAAAGGCATCAACTTGCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

528

Amino Acids

57.55

Weight (kDa)

5.0

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 264 - 465 2.4e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 437
Acc36I ACCTGC 3 cut(s) 437, 463, 1445
AccB7I CCANNNNNTGG 1 cut(s) 880
AccI GTMKAC 1 cut(s) 953
AciI CCGC 2 cut(s) 696, 1498
AclWI GGATC 2 cut(s) 894, 1106
AcsI RAATTY 3 cut(s) 131, 717, 1218
AcuI CTGAAG 2 cut(s) 60, 510
AfaI GTAC 2 cut(s) 1314, 1546
AfiI CCNNNNNNNGG 2 cut(s) 674, 880
AflII CTTAAG 2 cut(s) 175, 343
AgsI TTSAA 6 cut(s) 532, 738, 1153, 1186, 1226, 1276
AjnI CCWGG 1 cut(s) 873
Alw21I GWGCWC 2 cut(s) 835, 1284
Alw26I GTCTC 2 cut(s) 534, 1463
Alw44I GTGCAC 1 cut(s) 1280
AlwI GGATC 2 cut(s) 894, 1106
Ama87I CYCGRG 2 cut(s) 1161, 1421
ApaLI GTGCAC 1 cut(s) 1280
ApeKI GCWGC 4 cut(s) 170, 545, 1426, 1442
ApoI RAATTY 3 cut(s) 131, 717, 1218
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 1557
AspS9I GGNCC 2 cut(s) 452, 472
AsuHPI GGTGA 5 cut(s) 122, 276, 473, 980, 1010
AvaI CYCGRG 2 cut(s) 1161, 1421
AvaII GGWCC 2 cut(s) 452, 472
BaeGI GKGCMC 1 cut(s) 1284
BanII GRGCYC 1 cut(s) 835
BauI CACGAG 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 471
Bbv12I GWGCWC 2 cut(s) 835, 1284
BbvI GCAGC 4 cut(s) 157, 557, 1438, 1454
BccI CCATC 4 cut(s) 364, 995, 1207, 1319
BciT130I CCWGG 1 cut(s) 875
BcoDI GTCTC 2 cut(s) 534, 1463
BfaI CTAG 3 cut(s) 17, 243, 647
BfmI CTRYAG 1 cut(s) 1452
BfrI CTTAAG 2 cut(s) 175, 343
BfuAI ACCTGC 3 cut(s) 437, 463, 1445
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 4 cut(s) 171, 546, 1427, 1443
BlsI GCNGC 4 cut(s) 172, 547, 1428, 1444
BmcAI AGTACT 1 cut(s) 1314
Bme1390I CCNGG 1 cut(s) 875
Bme18I GGWCC 2 cut(s) 452, 472
BmeT110I CYCGRG 2 cut(s) 1161, 1421
BmgT120I GGNCC 2 cut(s) 452, 472
BmiI GGNNCC 1 cut(s) 473
BmrFI CCNGG 1 cut(s) 875
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 2 cut(s) 1195, 1577
BmuI ACTGGG 1 cut(s) 616
BpiI GAAGAC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 1405
BsaBI GATNNNNATC 1 cut(s) 709
BsaI GGTCTC 1 cut(s) 1463
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 2 cut(s) 674, 880
Bse1I ACTGG 1 cut(s) 611
Bse3DI GCAATG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 709
BseBI CCWGG 1 cut(s) 875
BseGI GGATG 2 cut(s) 710, 1102
BseJI GATNNNNATC 1 cut(s) 709
BseLI CCNNNNNNNGG 2 cut(s) 674, 880
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 2 cut(s) 837, 1474
BseNI ACTGG 1 cut(s) 611
BseSI GKGCMC 1 cut(s) 1284
BseXI GCAGC 4 cut(s) 157, 557, 1438, 1454
BseYI CCCAGC 2 cut(s) 560, 1540
BsiHKAI GWGCWC 2 cut(s) 835, 1284
BsiHKCI CYCGRG 2 cut(s) 1161, 1421
BsiSI CCGG 3 cut(s) 108, 1398, 1487
BslFI GGGAC 3 cut(s) 263, 577, 646
BslI CCNNNNNNNGG 2 cut(s) 674, 880
BsmAI GTCTC 2 cut(s) 534, 1463
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 3 cut(s) 263, 577, 646
BsmI GAATGC 1 cut(s) 766
Bso31I GGTCTC 1 cut(s) 1463
BsoBI CYCGRG 2 cut(s) 1161, 1421
Bsp1286I GDGCHC 2 cut(s) 835, 1284
Bsp143I GATC 3 cut(s) 643, 886, 1111
BspACI CCGC 2 cut(s) 696, 1498
BspCNI CTCAG 2 cut(s) 838, 1473
BspHI TCATGA 1 cut(s) 1476
BspLI GGNNCC 1 cut(s) 473
BspMAI CTGCAG 1 cut(s) 1456
BspMI ACCTGC 3 cut(s) 437, 463, 1445
BspPI GGATC 2 cut(s) 894, 1106
BspTI CTTAAG 2 cut(s) 175, 343
BspTNI GGTCTC 1 cut(s) 1463
BsrDI GCAATG 1 cut(s) 220
BsrI ACTGG 1 cut(s) 611
BssMI GATC 3 cut(s) 643, 886, 1111
BssNAI GTATAC 1 cut(s) 954
BssSI CACGAG 1 cut(s) 394
Bst1107I GTATAC 1 cut(s) 954
Bst2BI CACGAG 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 875
Bst4CI ACNGT 7 cut(s) 146, 288, 527, 570, 1063, 1123, 1525
Bst6I CTCTTC 2 cut(s) 286, 532
BstAFI CTTAAG 2 cut(s) 175, 343
BstAPI GCANNNNNTGC 1 cut(s) 811
BstDEI CTNAG 6 cut(s) 422, 756, 836, 846, 1410, 1460
BstF5I GGATG 2 cut(s) 710, 1102
BstHHI GCGC 1 cut(s) 1557
BstKTI GATC 3 cut(s) 646, 889, 1114
BstMAI GTCTC 2 cut(s) 534, 1463
BstMBI GATC 3 cut(s) 643, 886, 1111
BstMWI GCNNNNNNNGC 9 cut(s) 72, 87, 179, 194, 770, 811, 986, 1426, 1451
BstNI CCWGG 1 cut(s) 875
BstSCI CCNGG 1 cut(s) 873
BstSFI CTRYAG 1 cut(s) 1452
BstSLI GKGCMC 1 cut(s) 1284
BstV1I GCAGC 4 cut(s) 157, 557, 1438, 1454
BstV2I GAAGAC 1 cut(s) 471
BstZ17I GTATAC 1 cut(s) 954
BtsCI GGATG 2 cut(s) 710, 1102
BtsIMutI CAGTG 2 cut(s) 566, 1047
BveI ACCTGC 3 cut(s) 437, 463, 1445
CciI TCATGA 1 cut(s) 1476
CfoI GCGC 1 cut(s) 1557
Cfr13I GGNCC 2 cut(s) 452, 472
CseI GACGC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 1313, 1545
CviAII CATG 9 cut(s) 13, 430, 768, 806, 865, 938, 1117, 1465, 1477
CviQI GTAC 2 cut(s) 1313, 1545
DdeI CTNAG 6 cut(s) 422, 756, 836, 846, 1410, 1460
DpnI GATC 3 cut(s) 645, 888, 1113
DpnII GATC 3 cut(s) 643, 886, 1111
Eam1104I CTCTTC 2 cut(s) 286, 532
EarI CTCTTC 2 cut(s) 286, 532
EciI GGCGGA 1 cut(s) 1513
Ecl136II GAGCTC 1 cut(s) 833
Eco24I GRGCYC 1 cut(s) 835
Eco31I GGTCTC 1 cut(s) 1463
Eco47I GGWCC 2 cut(s) 452, 472
Eco53kI GAGCTC 1 cut(s) 833
Eco57I CTGAAG 2 cut(s) 60, 510
Eco88I CYCGRG 2 cut(s) 1161, 1421
EcoICRI GAGCTC 1 cut(s) 833
EcoRI GAATTC 2 cut(s) 131, 1218
EcoRII CCWGG 1 cut(s) 873
EcoT22I ATGCAT 2 cut(s) 866, 1210
EcoT38I GRGCYC 1 cut(s) 835
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 9 cut(s) 16, 433, 771, 809, 868, 941, 1120, 1468, 1480
FaqI GGGAC 3 cut(s) 263, 577, 646
FatI CATG 9 cut(s) 12, 429, 767, 805, 864, 937, 1116, 1464, 1476
FauI CCCGC 1 cut(s) 689
FauNDI CATATG 1 cut(s) 994
FblI GTMKAC 1 cut(s) 953
Fnu4HI GCNGC 4 cut(s) 171, 546, 1427, 1443
FokI GGATG 2 cut(s) 697, 1089
FriOI GRGCYC 1 cut(s) 835
Fsp4HI GCNGC 4 cut(s) 171, 546, 1427, 1443
FspBI CTAG 3 cut(s) 17, 243, 647
GlaI GCGC 1 cut(s) 1556
GluI GCNGC 4 cut(s) 171, 546, 1427, 1443
GsaI CCCAGC 2 cut(s) 564, 1544
HapII CCGG 3 cut(s) 108, 1398, 1487
HgaI GACGC 1 cut(s) 551
HhaI GCGC 1 cut(s) 1557
Hin1II CATG 9 cut(s) 16, 433, 771, 809, 868, 941, 1120, 1468, 1480
Hin6I GCGC 1 cut(s) 1555
HinP1I GCGC 1 cut(s) 1555
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 3 cut(s) 58, 633, 1364
HpaII CCGG 3 cut(s) 108, 1398, 1487
HphI GGTGA 5 cut(s) 122, 276, 473, 980, 1010
Hpy166II GTNNAC 5 cut(s) 452, 871, 954, 1100, 1282
Hpy188I TCNGA 5 cut(s) 137, 327, 373, 490, 1300
Hpy188III TCNNGA 8 cut(s) 405, 620, 647, 714, 1106, 1384, 1477, 1511
Hpy8I GTNNAC 5 cut(s) 452, 871, 954, 1100, 1282
Hpy99I CGWCG 1 cut(s) 1127
HpyAV CCTTC 8 cut(s) 16, 35, 132, 411, 674, 937, 1147, 1488
HpyCH4III ACNGT 7 cut(s) 146, 288, 527, 570, 1063, 1123, 1525
HpyCH4IV ACGT 1 cut(s) 1254
HpyF10VI GCNNNNNNNGC 9 cut(s) 72, 87, 179, 194, 770, 811, 986, 1426, 1451
HpyF3I CTNAG 6 cut(s) 422, 756, 836, 846, 1410, 1460
HpySE526I ACGT 1 cut(s) 1254
Hsp92II CATG 9 cut(s) 16, 433, 771, 809, 868, 941, 1120, 1468, 1480
HspAI GCGC 1 cut(s) 1555
Kzo9I GATC 3 cut(s) 643, 886, 1111
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 4 cut(s) 157, 557, 1438, 1454
LweI GCATC 2 cut(s) 1195, 1577
MaeI CTAG 3 cut(s) 17, 243, 647
MaeII ACGT 1 cut(s) 1254
MaeIII GTNAC 5 cut(s) 968, 1156, 1178, 1232, 1519
MalI GATC 3 cut(s) 645, 888, 1113
MboI GATC 3 cut(s) 643, 886, 1111
MboII GAAGA 7 cut(s) 273, 309, 476, 503, 549, 1214, 1481
MhlI GDGCHC 2 cut(s) 835, 1284
MlyI GAGTC 3 cut(s) 245, 406, 668
MmeI TCCRAC 3 cut(s) 282, 651, 850
MnlI CCTC 9 cut(s) 80, 94, 289, 763, 833, 1247, 1348, 1405, 1494
Mph1103I ATGCAT 2 cut(s) 866, 1210
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 9 cut(s) 33, 176, 344, 383, 594, 788, 960, 1010, 1031
MslI CAYNNNNRTG 2 cut(s) 434, 1515
MspA1I CMGCKG 1 cut(s) 548
MspCI CTTAAG 2 cut(s) 175, 343
MspI CCGG 3 cut(s) 108, 1398, 1487
MspR9I CCNGG 1 cut(s) 875
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 1 cut(s) 875
MwoI GCNNNNNNNGC 9 cut(s) 72, 87, 179, 194, 770, 811, 986, 1426, 1451
NdeI CATATG 1 cut(s) 994
NdeII GATC 3 cut(s) 643, 886, 1111
NlaIII CATG 9 cut(s) 16, 433, 771, 809, 868, 941, 1120, 1468, 1480
NlaIV GGNNCC 1 cut(s) 473
NmeAIII GCCGAG 1 cut(s) 1001
NmuCI GTSAC 2 cut(s) 968, 1156
NsiI ATGCAT 2 cut(s) 866, 1210
PaeR7I CTCGAG 2 cut(s) 1161, 1421
PagI TCATGA 1 cut(s) 1476
PaqCI CACCTGC 1 cut(s) 437
PctI GAATGC 1 cut(s) 766
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 7 cut(s) 104, 368, 374, 611, 623, 734, 794
PflFI GACNNNGTC 1 cut(s) 470
PflMI CCANNNNNTGG 1 cut(s) 880
PkrI GCNGC 4 cut(s) 172, 547, 1428, 1444
PleI GAGTC 3 cut(s) 245, 405, 667
PpsI GAGTC 3 cut(s) 245, 405, 667
Psp124BI GAGCTC 1 cut(s) 835
Psp6I CCWGG 1 cut(s) 873
PspFI CCCAGC 2 cut(s) 560, 1540
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 1 cut(s) 473
PspPI GGNCC 2 cut(s) 452, 472
PspXI VCTCGAGB 2 cut(s) 1161, 1421
PstI CTGCAG 1 cut(s) 1456
PsyI GACNNNGTC 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 2 cut(s) 1314, 1546
RsaNI GTAC 2 cut(s) 1313, 1545
RseI CAYNNNNRTG 2 cut(s) 434, 1515
SacI GAGCTC 1 cut(s) 835
SaqAI TTAA 9 cut(s) 33, 176, 344, 383, 594, 788, 960, 1010, 1031
SatI GCNGC 4 cut(s) 171, 546, 1427, 1443
Sau3AI GATC 3 cut(s) 643, 886, 1111
Sau96I GGNCC 2 cut(s) 452, 472
ScaI AGTACT 1 cut(s) 1314
SchI GAGTC 3 cut(s) 245, 406, 668
ScrFI CCNGG 1 cut(s) 875
SduI GDGCHC 2 cut(s) 835, 1284
SfaNI GCATC 2 cut(s) 1195, 1577
SfcI CTRYAG 1 cut(s) 1452
Sfr274I CTCGAG 2 cut(s) 1161, 1421
SinI GGWCC 2 cut(s) 452, 472
SlaI CTCGAG 2 cut(s) 1161, 1421
SmiMI CAYNNNNRTG 2 cut(s) 434, 1515
SmlI CTYRAG 5 cut(s) 175, 343, 1161, 1384, 1421
SmoI CTYRAG 5 cut(s) 175, 343, 1161, 1384, 1421
SsiI CCGC 2 cut(s) 696, 1498
SspMI CTAG 3 cut(s) 17, 243, 647
SstI GAGCTC 1 cut(s) 835
StyD4I CCNGG 1 cut(s) 873
TaaI ACNGT 7 cut(s) 146, 288, 527, 570, 1063, 1123, 1525
TaiI ACGT 1 cut(s) 1257
TaqI TCGA 3 cut(s) 338, 1162, 1422
TatI WGTACW 1 cut(s) 1312
TfiI GAWTC 7 cut(s) 104, 368, 374, 611, 623, 734, 794
Tru1I TTAA 9 cut(s) 33, 176, 344, 383, 594, 788, 960, 1010, 1031
Tru9I TTAA 9 cut(s) 33, 176, 344, 383, 594, 788, 960, 1010, 1031
TscAI CASTG 2 cut(s) 573, 1054
TseFI GTSAC 2 cut(s) 968, 1156
TseI GCWGC 4 cut(s) 170, 545, 1426, 1442
Tsp45I GTSAC 2 cut(s) 968, 1156
TspDTI ATGAA 7 cut(s) 132, 252, 401, 588, 588, 699, 1493
TspRI CASTG 2 cut(s) 573, 1054
Tth111I GACNNNGTC 1 cut(s) 470
Van91I CCANNNNNTGG 1 cut(s) 880
Vha464I CTTAAG 2 cut(s) 175, 343
VneI GTGCAC 1 cut(s) 1280
VpaK11BI GGWCC 2 cut(s) 452, 472
XapI RAATTY 3 cut(s) 131, 717, 1218
XbaI TCTAGA 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 1123
XhoI CTCGAG 2 cut(s) 1161, 1421
XmiI GTMKAC 1 cut(s) 953
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 3 cut(s) 17, 243, 647
ZrmI AGTACT 1 cut(s) 1314
Zsp2I ATGCAT 2 cut(s) 866, 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.