Rw4G020500

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008027
Physical Location & Seq
Reverse (-)
44508703 .. 44512103
3401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020500.1

Sequence Viewer

Length: 1416 bp
ATGAGAGTCCATATAGAAGTGGGAAACGGCAATACCGGTAAGAGAGTCATGCTGGGAGAAACTATGATTAAACCTGAAGGAGAACTTAAAGTAAGCTTTGGTTATCAATGCAGTAGTGCAGAGAACAGTAGTTTCTCTTGCTTGTCTGGAGCTGCGTTAAGTGCCAATGCCACTCTGGCAAACACTAATATCTGCAATGGTTTGATAGGGGAAGAAATACTTCCTAGTTGGGACTCCCCTAATTCATTTCGTAAGGTACCCTCTTCGCCAAGGTTGGATATATTATCATCTTCTCTTCAGAGTAGTATGTCAAACTTAAGTTGCAGTCCATCTACACCGGAGTCTGATTCTTACTTGATGAAATCCATGAGTCCTACTTCCAGAGGTGAAGGTTTTCTCAATTCCATGGAAGTACAAGTGGCAGGTGGAGCTGCTGGTGAAGACAGGGTCCAAGCGGTTTGTTCTGAAGAAAATGGGTGGCTGTATTATTGGGAATCAAATCAGGAATCCATCAATGCTTCTAAAGGGCTAAATATTGATGGGTCTCGTCCATTTATTGAAGATGATAGTAATATTGAAAATATCTCCAAGGATGCTTCATCTACTGACGTAGAAATGTCAACTGATTCATTCAGGCATGGGGTACTTGATAACCTACAACGAGCTCTTAGTCAGGCCGAGAATGATTTTCTATACATGGTTGAGCAGGAAATGGAGGATCGTCCTGATTTGGTTTCTGTTGGGTCTTGTGTTTTAGTTGTCCTTCTTCATGGGAATGATTTGTATACACTTAATTTAGGTGACAGCCGAGCTGTGTTGGCAACACTTGGTGATGGTGATTTCATGAATGGGAGTGAGAGACTGAAAGCTATCCAGCTCACTGAAAGTCATACGGTTGATGATGAAGTTGAAAGAATGCGAGTTTTGTGTGATCATCCTGATGACCCCATGTCCATTGTGGCTGGAAAAGTAAAAGGGAAATTGAAGGTCACTCGAGCTTTTGGAGTTGGTTACTTGAAAAAGAAAAAACTGAATGATGCATTGATGGGGATTCTTCAAGTTCGTAATCTAATAAGCCCACCATATGTTTCTACTCAACCATCATTGAGCGTGCGTAGAATATCAAAGTCGGATCGATTTGCCATAGTTGCAAGTGATGGTTTGTTTGACTTTTTTACCAATGACGAGGCAGTAAAGCTTGTCCATTCCTATATCTTGAGCAACCCCTCTGGTGATCCATCTAAGTTTCTGCTGGAGCAGCTTGTAGTGAAAGCAGCCAACTGTGCAGGATTCAGTATGGAAGAACTAATGAATATACCAGCGGGGAGGAGGAGGAAGTATCATGATGATGTAACTGTAATTGTGGTTATTCTTGGTACAAATCAGCGCACCACAAAGGCATCAACTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

471

Amino Acids

51.3

Weight (kDa)

5.0

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 210 - 409 1e-30 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 413
Acc65I GGTACC 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 785
AciI CCGC 2 cut(s) 455, 1322
AclWI GGATC 3 cut(s) 726, 1140, 1229
AcuI CTGAAG 3 cut(s) 96, 281, 486
AdeI CACNNNGTG 1 cut(s) 830
AfaI GTAC 4 cut(s) 258, 414, 645, 1378
AflII CTTAAG 1 cut(s) 316
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 6 cut(s) 560, 578, 911, 985, 1018, 1058
AhdI GACNNNNNGTC 1 cut(s) 949
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 2 cut(s) 549, 853
AlwI GGATC 3 cut(s) 726, 1140, 1229
Ama87I CYCGRG 1 cut(s) 993
AoxI GGCC 1 cut(s) 675
ApeKI GCWGC 4 cut(s) 152, 431, 1258, 1274
AsiGI ACCGGT 1 cut(s) 35
Asp700I GAANNNNTTC 2 cut(s) 219, 393
Asp718I GGTACC 1 cut(s) 256
AspLEI GCGC 1 cut(s) 1389
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 6 cut(s) 398, 449, 812, 842, 848, 1244
AvaI CYCGRG 1 cut(s) 993
AvaII GGWCC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 4 cut(s) 139, 418, 1270, 1286
BccI CCATC 8 cut(s) 337, 518, 533, 827, 1039, 1108, 1151, 1246
BceAI ACGGC 1 cut(s) 43
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 2 cut(s) 549, 853
BfaI CTAG 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 413
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 4 cut(s) 153, 432, 1259, 1275
BlsI GCNGC 4 cut(s) 154, 433, 1260, 1276
Bme18I GGWCC 1 cut(s) 448
BmeRI GACNNNNNGTC 1 cut(s) 949
BmeT110I CYCGRG 1 cut(s) 993
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 2 cut(s) 258, 449
BmsI GCATC 3 cut(s) 583, 1027, 1409
BpiI GAAGAC 1 cut(s) 447
BpmI CTGGAG 2 cut(s) 168, 1274
BpuEI CTTGAG 1 cut(s) 1237
Bsa29I ATCGAT 1 cut(s) 1135
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 3 cut(s) 269, 405, 588
BsaWI WCCGGW 2 cut(s) 35, 337
Bse118I RCCGGY 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 202
BseCI ATCGAT 1 cut(s) 1135
BseDI CCNNGG 3 cut(s) 269, 405, 588
BseGI GGATG 2 cut(s) 598, 934
BseMI GCAATG 1 cut(s) 202
BseRI GAGGAG 2 cut(s) 1342, 1345
BseXI GCAGC 4 cut(s) 139, 418, 1270, 1286
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 2 cut(s) 138, 1305
BshFI GGCC 1 cut(s) 677
BshNI GGYRCC 1 cut(s) 256
BshTI ACCGGT 1 cut(s) 35
BshVI ATCGAT 1 cut(s) 1135
BsiHKAI GWGCWC 1 cut(s) 667
BsiHKCI CYCGRG 1 cut(s) 993
BsiSI CCGG 2 cut(s) 36, 338
BslFI GGGAC 1 cut(s) 245
BsmAI GTCTC 2 cut(s) 549, 853
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 1 cut(s) 677
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 993
Bsp1286I GDGCHC 1 cut(s) 667
Bsp143I GATC 4 cut(s) 718, 931, 1132, 1234
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 2 cut(s) 455, 1322
BspANI GGCC 1 cut(s) 677
BspDI ATCGAT 1 cut(s) 1135
BspHI TCATGA 2 cut(s) 843, 1342
BspLI GGNNCC 2 cut(s) 258, 449
BspMI ACCTGC 1 cut(s) 413
BspPI GGATC 3 cut(s) 726, 1140, 1229
BspT107I GGYRCC 1 cut(s) 256
BspTI CTTAAG 1 cut(s) 316
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 269, 405, 588
BssMI GATC 4 cut(s) 718, 931, 1132, 1234
BssNAI GTATAC 1 cut(s) 786
BssT1I CCWWGG 3 cut(s) 269, 405, 588
Bst1107I GTATAC 1 cut(s) 786
Bst4CI ACNGT 4 cut(s) 128, 895, 1283, 1357
Bst6I CTCTTC 2 cut(s) 268, 300
BstAFI CTTAAG 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 1112
BstDEI CTNAG 2 cut(s) 668, 1242
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 598, 934
BstHHI GCGC 1 cut(s) 1389
BstKTI GATC 4 cut(s) 721, 934, 1135, 1237
BstMAI GTCTC 2 cut(s) 549, 853
BstMBI GATC 4 cut(s) 718, 931, 1132, 1234
BstMWI GCNNNNNNNGC 7 cut(s) 161, 176, 428, 818, 1148, 1258, 1283
BstV1I GCAGC 4 cut(s) 139, 418, 1270, 1286
BstV2I GAAGAC 1 cut(s) 447
BstZ17I GTATAC 1 cut(s) 786
Bsu15I ATCGAT 1 cut(s) 1135
BsuRI GGCC 1 cut(s) 677
BsuTUI ATCGAT 1 cut(s) 1135
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 2 cut(s) 598, 934
BtsIMutI CAGTG 1 cut(s) 879
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 1 cut(s) 1112
CciI TCATGA 2 cut(s) 843, 1342
CfoI GCGC 1 cut(s) 1389
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 448
ClaI ATCGAT 1 cut(s) 1135
Csp6I GTAC 4 cut(s) 257, 413, 644, 1377
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 9 cut(s) 49, 367, 406, 638, 697, 770, 844, 949, 1343
CviQI GTAC 4 cut(s) 257, 413, 644, 1377
DdeI CTNAG 2 cut(s) 668, 1242
DpnI GATC 4 cut(s) 720, 933, 1134, 1236
DpnII GATC 4 cut(s) 718, 931, 1132, 1234
DraIII CACNNNGTG 1 cut(s) 830
DriI GACNNNNNGTC 1 cut(s) 949
Eam1104I CTCTTC 2 cut(s) 268, 300
Eam1105I GACNNNNNGTC 1 cut(s) 949
EarI CTCTTC 2 cut(s) 268, 300
Ecl136II GAGCTC 1 cut(s) 665
Eco130I CCWWGG 3 cut(s) 269, 405, 588
Eco24I GRGCYC 1 cut(s) 667
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 448
Eco53kI GAGCTC 1 cut(s) 665
Eco57I CTGAAG 3 cut(s) 96, 281, 486
Eco88I CYCGRG 1 cut(s) 993
EcoICRI GAGCTC 1 cut(s) 665
EcoT14I CCWWGG 3 cut(s) 269, 405, 588
EcoT22I ATGCAT 1 cut(s) 1042
EcoT38I GRGCYC 1 cut(s) 667
ErhI CCWWGG 3 cut(s) 269, 405, 588
FaeI CATG 9 cut(s) 52, 370, 409, 641, 700, 773, 847, 952, 1346
FalI AAGNNNNNCTT 4 cut(s) 69, 101, 204, 236
FaqI GGGAC 1 cut(s) 245
FatI CATG 9 cut(s) 48, 366, 405, 637, 696, 769, 843, 948, 1342
FauI CCCGC 1 cut(s) 1315
FauNDI CATATG 1 cut(s) 1084
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 1 cut(s) 785
Fnu4HI GCNGC 4 cut(s) 153, 432, 1259, 1275
FokI GGATG 2 cut(s) 605, 921
FriOI GRGCYC 1 cut(s) 667
Fsp4HI GCNGC 4 cut(s) 153, 432, 1259, 1275
FspBI CTAG 1 cut(s) 225
GlaI GCGC 1 cut(s) 1388
GluI GCNGC 4 cut(s) 153, 432, 1259, 1275
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 2 cut(s) 168, 1274
HaeIII GGCC 1 cut(s) 677
HapII CCGG 2 cut(s) 36, 338
HhaI GCGC 1 cut(s) 1389
Hin1II CATG 9 cut(s) 52, 370, 409, 641, 700, 773, 847, 952, 1346
Hin6I GCGC 1 cut(s) 1387
HinP1I GCGC 1 cut(s) 1387
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 2 cut(s) 94, 1196
HpaII CCGG 2 cut(s) 36, 338
HphI GGTGA 6 cut(s) 398, 449, 812, 842, 848, 1244
Hpy166II GTNNAC 2 cut(s) 621, 786
Hpy188I TCNGA 4 cut(s) 300, 346, 466, 1132
Hpy188III TCNNGA 8 cut(s) 147, 381, 503, 725, 844, 938, 1216, 1343
Hpy8I GTNNAC 2 cut(s) 621, 786
HpyAV CCTTC 4 cut(s) 71, 383, 773, 979
HpyCH4III ACNGT 4 cut(s) 128, 895, 1283, 1357
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 7 cut(s) 111, 119, 195, 324, 1040, 1151, 1286
HpyF10VI GCNNNNNNNGC 7 cut(s) 161, 176, 428, 818, 1148, 1258, 1283
HpyF3I CTNAG 2 cut(s) 668, 1242
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 9 cut(s) 52, 370, 409, 641, 700, 773, 847, 952, 1346
HspAI GCGC 1 cut(s) 1387
KpnI GGTACC 1 cut(s) 260
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 4 cut(s) 718, 931, 1132, 1234
LmnI GCTCC 3 cut(s) 149, 428, 1255
Lsp1109I GCAGC 4 cut(s) 139, 418, 1270, 1286
LweI GCATC 3 cut(s) 583, 1027, 1409
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 4 cut(s) 800, 988, 1010, 1351
MalI GATC 4 cut(s) 720, 933, 1134, 1236
MboI GATC 4 cut(s) 718, 931, 1132, 1234
MhlI GDGCHC 1 cut(s) 667
MluCI AATT 5 cut(s) 241, 400, 793, 980, 1359
MlyI GAGTC 5 cut(s) 15, 54, 227, 350, 379
MmeI TCCRAC 2 cut(s) 255, 1110
MnlI CCTC 8 cut(s) 271, 377, 709, 1180, 1237, 1320, 1323, 1326
Mph1103I ATGCAT 1 cut(s) 1042
MroXI GAANNNNTTC 2 cut(s) 219, 393
MseI TTAA 5 cut(s) 69, 87, 158, 317, 792
MslI CAYNNNNRTG 3 cut(s) 774, 939, 1347
MspA1I CMGCKG 1 cut(s) 1322
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 2 cut(s) 36, 338
Mva1269I GAATGC 1 cut(s) 921
MwoI GCNNNNNNNGC 7 cut(s) 161, 176, 428, 818, 1148, 1258, 1283
NcoI CCATGG 1 cut(s) 405
NdeI CATATG 1 cut(s) 1084
NdeII GATC 4 cut(s) 718, 931, 1132, 1234
NlaIII CATG 9 cut(s) 52, 370, 409, 641, 700, 773, 847, 952, 1346
NlaIV GGNNCC 2 cut(s) 258, 449
NmeAIII GCCGAG 2 cut(s) 703, 833
NmuCI GTSAC 2 cut(s) 800, 988
NsiI ATGCAT 1 cut(s) 1042
PaeR7I CTCGAG 1 cut(s) 993
PagI TCATGA 2 cut(s) 843, 1342
PaqCI CACCTGC 1 cut(s) 413
PctI GAATGC 1 cut(s) 921
PdmI GAANNNNTTC 2 cut(s) 219, 393
PfeI GAWTC 6 cut(s) 347, 494, 506, 626, 1051, 1290
PflFI GACNNNGTC 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 4 cut(s) 154, 433, 1260, 1276
PleI GAGTC 5 cut(s) 14, 53, 227, 349, 378
PpsI GAGTC 5 cut(s) 14, 53, 227, 349, 378
Psp124BI GAGCTC 1 cut(s) 667
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 258, 449
PspPI GGNCC 1 cut(s) 448
PspXI VCTCGAGB 1 cut(s) 993
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 4 cut(s) 258, 414, 645, 1378
RsaNI GTAC 4 cut(s) 257, 413, 644, 1377
RseI CAYNNNNRTG 3 cut(s) 774, 939, 1347
SacI GAGCTC 1 cut(s) 667
SaqAI TTAA 5 cut(s) 69, 87, 158, 317, 792
SatI GCNGC 4 cut(s) 153, 432, 1259, 1275
Sau3AI GATC 4 cut(s) 718, 931, 1132, 1234
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 5 cut(s) 15, 54, 227, 350, 379
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 3 cut(s) 583, 1027, 1409
Sfr274I CTCGAG 1 cut(s) 993
SinI GGWCC 1 cut(s) 448
SlaI CTCGAG 1 cut(s) 993
SmiMI CAYNNNNRTG 3 cut(s) 774, 939, 1347
SmlI CTYRAG 3 cut(s) 316, 993, 1216
SmoI CTYRAG 3 cut(s) 316, 993, 1216
Sse9I AATT 5 cut(s) 241, 400, 793, 980, 1359
SsiI CCGC 2 cut(s) 455, 1322
SspI AATATT 2 cut(s) 535, 574
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 667
StyI CCWWGG 3 cut(s) 269, 405, 588
TaaI ACNGT 4 cut(s) 128, 895, 1283, 1357
TaiI ACGT 1 cut(s) 612
TaqI TCGA 2 cut(s) 994, 1135
TasI AATT 5 cut(s) 241, 400, 793, 980, 1359
TatI WGTACW 1 cut(s) 412
TfiI GAWTC 6 cut(s) 347, 494, 506, 626, 1051, 1290
Tru1I TTAA 5 cut(s) 69, 87, 158, 317, 792
Tru9I TTAA 5 cut(s) 69, 87, 158, 317, 792
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 2 cut(s) 800, 988
TseI GCWGC 4 cut(s) 152, 431, 1258, 1274
Tsp45I GTSAC 2 cut(s) 800, 988
TspDTI ATGAA 9 cut(s) 234, 374, 588, 618, 758, 832, 860, 918, 1325
TspRI CASTG 1 cut(s) 886
Tth111I GACNNNGTC 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 2 cut(s) 172, 955
XhoI CTCGAG 1 cut(s) 993
XmiI GTMKAC 1 cut(s) 785
XmnI GAANNNNTTC 2 cut(s) 219, 393
XspI CTAG 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.