RLG00000007634

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008027
Physical Location & Seq
Forward (+)
19251221 .. 19254911
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007634

Sequence Viewer

Length: 1515 bp
ATGCTGGGAGAAACTATGATTAAACCTGAAGGAGAACTTAAAGTAAGCTTTGGCTATCAATGCAGTAGTGACAGAGTCGATTCTTGTGAGGCTTCCAGTGGATATGAAATCCTGCCTGACCTCCGAAGGAACAGTAGTTTCTCTTGCTTGTCTGGAGCTGCGTTAAGTGCCAATGCCACTCTGGCAAACACTAATATCTGCAATGGTTTGATAGGGGAAGAAATACTTCCTAGTTGGGACTCCCCTAATTCATTTCGTAAGGTACCCTCTTCGCCAAGGTTGGATATATTATCATCTTCTCTTCAGAGTAGTATGTCAAACTTAAGTTGCAGTCCATCTACACCCGAGTCTGATTCTTACTTGATGAAATCCATGAGTCCTACTTCCAGAGGTGAAGGTTTTCTCAATTCCATGGAAGTACAAGTGGCAGGTGGAGCTGCTGGTGAAGACAGGGTCCAAGCGGTTTGTTCTGAAGAAAATGGGTGGCTGTATTGTGCAATATACGATGGCTTTAATGGAAGAGACGCAGCTGATTTTCTGGCTGGGACACTGTATGAAACCATTGTATTTTACTTTAATTCATTAGACTGGGAATCAAATCAGGAATCCATCAATGCTTCTAAAGGGCTAAATATTGATGGGTCTCGTCCATTTATTGAAGATGATAGTAATATTGAAAATATCTCCAAGGATACTTCATCTACTGAAGTAGAAATGTCAATTGATTCATTCAGGCATGGGGTACTTGATAGCCTACAACGAGCTCTTAGTCAGGCCGAGAATGATTTTCTATACATGGTTGAGCAGGAAATGGAGGATCGTCCTGATTTGGTTTCTGTTGGGTCTTGTGTTTTAGTTGTGCTTCTTCATGGGAATGATTTGTATACACTGAATTTAGGTGACAGCCGGGCTGTGTTGGCAACACTTGGTGATGGTGATTTCATGAATGGGAGTGAGAGACTGAAAGCTATCCAGCTCACTGAAAATCATACGGTTGATGAGGAAGTTGAAAGAACGAGAGTTTTGTGTGATCATCCTGATGACCCCATGTCCATTGTGGCTGGAAAAGTAAAAGGGAAATTGAAGGTCACTCGAGCTTTTGGAGTTGGTTACTTGAAAAAGAAAAAACTGAATGATGCATTGATGGGGATTCTTCAAGTTCGTAATCTAATAAGCCCACCTTATGTTTCTACTCAACCATCATTGAGCGTGCGTAGAATATCAAAGTCGGATCGATTTGTCATAGTTGCAAGTGATGGTTTGTTTGACTTTTTTACCAATGACGAGGCAGTAAAGCTTGTCCATTCCTATATCTTGAGCAACCCCTCTGGTGATCCATCCAAGTTTCTGTTGGAGCAGCTTGTAGTGAAAGCAGCCAACCGTGCAGGATTCAGTATGGAAGAACTAATGAATATACCAGCGGGGAGGAGGAGGAAGTATCATGATGATGTAACTGTAATTGTGGTTATTCTTGGTACAAATCAGCGCACCACAAAGGCATCAACTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

505

Amino Acids

55.14

Weight (kDa)

4.78

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 243 - 445 3.1e-31 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 419
Acc65I GGTACC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 262
AccI GTMKAC 1 cut(s) 884
AciI CCGC 2 cut(s) 461, 1421
AclWI GGATC 3 cut(s) 825, 1239, 1328
AcsI RAATTY 1 cut(s) 892
AcuI CTGAAG 4 cut(s) 48, 287, 492, 726
AdeI CACNNNGTG 1 cut(s) 929
AfaI GTAC 4 cut(s) 264, 420, 744, 1477
AflII CTTAAG 1 cut(s) 322
AgsI TTSAA 6 cut(s) 659, 677, 1010, 1084, 1117, 1157
AhdI GACNNNNNGTC 1 cut(s) 1048
Alw21I GWGCWC 1 cut(s) 766
Alw26I GTCTC 3 cut(s) 516, 648, 952
AlwI GGATC 3 cut(s) 825, 1239, 1328
Ama87I CYCGRG 2 cut(s) 344, 1092
AoxI GGCC 1 cut(s) 774
ApeKI GCWGC 5 cut(s) 158, 437, 527, 1357, 1373
ApoI RAATTY 1 cut(s) 892
Asp700I GAANNNNTTC 2 cut(s) 225, 399
Asp718I GGTACC 1 cut(s) 262
AspLEI GCGC 1 cut(s) 1488
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 1 cut(s) 908
AsuHPI GGTGA 6 cut(s) 404, 455, 911, 941, 947, 1343
AvaI CYCGRG 2 cut(s) 344, 1092
AvaII GGWCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 766
BbvI GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
BccI CCATC 9 cut(s) 343, 500, 617, 632, 926, 1138, 1207, 1250, 1345
BciVI GTATCC 1 cut(s) 685
BclI TGATCA 1 cut(s) 1030
BcnI CCSGG 1 cut(s) 908
BcoDI GTCTC 3 cut(s) 516, 648, 952
BfaI CTAG 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 322
BfuAI ACCTGC 1 cut(s) 419
BfuI GTATCC 1 cut(s) 685
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
BlsI GCNGC 5 cut(s) 160, 439, 529, 1359, 1375
Bme1390I CCNGG 1 cut(s) 908
Bme18I GGWCC 1 cut(s) 454
BmeRI GACNNNNNGTC 1 cut(s) 1048
BmeT110I CYCGRG 2 cut(s) 344, 1092
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 2 cut(s) 264, 455
BmrFI CCNGG 1 cut(s) 908
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 2 cut(s) 1126, 1508
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 453
BpmI CTGGAG 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 1336
BpuMI CCSGG 1 cut(s) 908
Bsa29I ATCGAT 1 cut(s) 1234
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 275, 411, 687
Bse1I ACTGG 2 cut(s) 96, 593
Bse3DI GCAATG 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 1234
BseDI CCNNGG 3 cut(s) 275, 411, 687
BseGI GGATG 2 cut(s) 1033, 1337
BseMI GCAATG 1 cut(s) 208
BseNI ACTGG 2 cut(s) 96, 593
BseRI GAGGAG 2 cut(s) 1441, 1444
BseXI GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
BseYI CCCAGC 2 cut(s) 4, 542
BsgI GTGCAG 1 cut(s) 1404
BshFI GGCC 1 cut(s) 776
BshNI GGYRCC 1 cut(s) 262
BshVI ATCGAT 1 cut(s) 1234
BsiHKAI GWGCWC 1 cut(s) 766
BsiHKCI CYCGRG 2 cut(s) 344, 1092
BsiSI CCGG 1 cut(s) 907
BslFI GGGAC 2 cut(s) 251, 559
BsmAI GTCTC 3 cut(s) 516, 648, 952
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 2 cut(s) 251, 559
BsnI GGCC 1 cut(s) 776
Bso31I GGTCTC 1 cut(s) 648
BsoBI CYCGRG 2 cut(s) 344, 1092
Bsp1286I GDGCHC 1 cut(s) 766
Bsp143I GATC 4 cut(s) 817, 1030, 1231, 1333
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 2 cut(s) 461, 1421
BspANI GGCC 1 cut(s) 776
BspDI ATCGAT 1 cut(s) 1234
BspHI TCATGA 2 cut(s) 942, 1441
BspLI GGNNCC 2 cut(s) 264, 455
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 3 cut(s) 825, 1239, 1328
BspT107I GGYRCC 1 cut(s) 262
BspTI CTTAAG 1 cut(s) 322
BspTNI GGTCTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 2 cut(s) 96, 593
BssECI CCNNGG 3 cut(s) 275, 411, 687
BssMI GATC 4 cut(s) 817, 1030, 1231, 1333
BssNAI GTATAC 1 cut(s) 885
BssT1I CCWWGG 3 cut(s) 275, 411, 687
Bst1107I GTATAC 1 cut(s) 885
Bst4CI ACNGT 5 cut(s) 134, 552, 994, 1382, 1456
Bst6I CTCTTC 3 cut(s) 274, 306, 514
BstAFI CTTAAG 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 1211
BstDEI CTNAG 1 cut(s) 767
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 2 cut(s) 1033, 1337
BstHHI GCGC 1 cut(s) 1488
BstKTI GATC 4 cut(s) 820, 1033, 1234, 1336
BstMAI GTCTC 3 cut(s) 516, 648, 952
BstMBI GATC 4 cut(s) 817, 1030, 1231, 1333
BstMWI GCNNNNNNNGC 6 cut(s) 60, 167, 182, 434, 917, 1382
BstSCI CCNGG 1 cut(s) 906
BstV1I GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
BstV2I GAAGAC 1 cut(s) 453
BstZ17I GTATAC 1 cut(s) 885
Bsu15I ATCGAT 1 cut(s) 1234
BsuI GTATCC 1 cut(s) 685
BsuRI GGCC 1 cut(s) 776
BsuTUI ATCGAT 1 cut(s) 1234
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 2 cut(s) 1033, 1337
BtsIMutI CAGTG 4 cut(s) 103, 548, 887, 978
BveI ACCTGC 1 cut(s) 419
Cac8I GCNNGC 1 cut(s) 1211
CciI TCATGA 2 cut(s) 942, 1441
CfoI GCGC 1 cut(s) 1488
Cfr13I GGNCC 1 cut(s) 454
ClaI ATCGAT 1 cut(s) 1234
CseI GACGC 1 cut(s) 533
Csp6I GTAC 4 cut(s) 263, 419, 743, 1476
CviAII CATG 8 cut(s) 373, 412, 737, 796, 869, 943, 1048, 1442
CviQI GTAC 4 cut(s) 263, 419, 743, 1476
DdeI CTNAG 1 cut(s) 767
DpnI GATC 4 cut(s) 819, 1032, 1233, 1335
DpnII GATC 4 cut(s) 817, 1030, 1231, 1333
DraIII CACNNNGTG 1 cut(s) 929
DriI GACNNNNNGTC 1 cut(s) 1048
Eam1104I CTCTTC 3 cut(s) 274, 306, 514
Eam1105I GACNNNNNGTC 1 cut(s) 1048
EarI CTCTTC 3 cut(s) 274, 306, 514
Ecl136II GAGCTC 1 cut(s) 764
Eco130I CCWWGG 3 cut(s) 275, 411, 687
Eco24I GRGCYC 1 cut(s) 766
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 764
Eco57I CTGAAG 4 cut(s) 48, 287, 492, 726
Eco88I CYCGRG 2 cut(s) 344, 1092
EcoICRI GAGCTC 1 cut(s) 764
EcoT14I CCWWGG 3 cut(s) 275, 411, 687
EcoT22I ATGCAT 1 cut(s) 1141
EcoT38I GRGCYC 1 cut(s) 766
ErhI CCWWGG 3 cut(s) 275, 411, 687
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 8 cut(s) 376, 415, 740, 799, 872, 946, 1051, 1445
FalI AAGNNNNNCTT 6 cut(s) 21, 53, 210, 242, 1165, 1197
FaqI GGGAC 2 cut(s) 251, 559
FatI CATG 8 cut(s) 372, 411, 736, 795, 868, 942, 1047, 1441
FauI CCCGC 1 cut(s) 1414
FbaI TGATCA 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 884
Fnu4HI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
FokI GGATG 2 cut(s) 1020, 1324
FriOI GRGCYC 1 cut(s) 766
Fsp4HI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 1487
GluI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
GsaI CCCAGC 2 cut(s) 8, 546
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 1 cut(s) 776
HapII CCGG 1 cut(s) 907
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 1488
Hin1II CATG 8 cut(s) 376, 415, 740, 799, 872, 946, 1051, 1445
Hin6I GCGC 1 cut(s) 1486
HinP1I GCGC 1 cut(s) 1486
HindIII AAGCTT 2 cut(s) 46, 1295
HpaII CCGG 1 cut(s) 907
HphI GGTGA 6 cut(s) 404, 455, 911, 941, 947, 1343
Hpy166II GTNNAC 1 cut(s) 885
Hpy188I TCNGA 5 cut(s) 125, 306, 352, 472, 1231
Hpy188III TCNNGA 8 cut(s) 153, 387, 602, 824, 943, 1037, 1315, 1442
Hpy8I GTNNAC 1 cut(s) 885
HpyAV CCTTC 4 cut(s) 23, 120, 389, 1078
HpyCH4III ACNGT 5 cut(s) 134, 552, 994, 1382, 1456
HpyCH4V TGCA 7 cut(s) 63, 201, 330, 497, 1139, 1250, 1385
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 167, 182, 434, 917, 1382
HpyF3I CTNAG 1 cut(s) 767
Hsp92II CATG 8 cut(s) 376, 415, 740, 799, 872, 946, 1051, 1445
HspAI GCGC 1 cut(s) 1486
KpnI GGTACC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 1030
Kzo9I GATC 4 cut(s) 817, 1030, 1231, 1333
LmnI GCTCC 3 cut(s) 155, 434, 1354
Lsp1109I GCAGC 5 cut(s) 145, 424, 539, 1369, 1385
LweI GCATC 2 cut(s) 1126, 1508
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 5 cut(s) 68, 899, 1087, 1109, 1450
MalI GATC 4 cut(s) 819, 1032, 1233, 1335
MboI GATC 4 cut(s) 817, 1030, 1231, 1333
MfeI CAATTG 1 cut(s) 720
MhlI GDGCHC 1 cut(s) 766
MluCI AATT 7 cut(s) 247, 406, 577, 720, 892, 1079, 1458
MlyI GAGTC 4 cut(s) 84, 233, 356, 385
MmeI TCCRAC 3 cut(s) 261, 1209, 1332
Mph1103I ATGCAT 1 cut(s) 1141
MroXI GAANNNNTTC 2 cut(s) 225, 399
MseI TTAA 6 cut(s) 21, 39, 164, 323, 513, 576
MslI CAYNNNNRTG 3 cut(s) 873, 1038, 1446
MspA1I CMGCKG 2 cut(s) 530, 1421
MspCI CTTAAG 1 cut(s) 322
MspI CCGG 1 cut(s) 907
MspR9I CCNGG 1 cut(s) 908
MunI CAATTG 1 cut(s) 720
MwoI GCNNNNNNNGC 6 cut(s) 60, 167, 182, 434, 917, 1382
NciI CCSGG 1 cut(s) 908
NcoI CCATGG 1 cut(s) 411
NdeII GATC 4 cut(s) 817, 1030, 1231, 1333
NlaIII CATG 8 cut(s) 376, 415, 740, 799, 872, 946, 1051, 1445
NlaIV GGNNCC 2 cut(s) 264, 455
NmeAIII GCCGAG 1 cut(s) 802
NmuCI GTSAC 3 cut(s) 68, 899, 1087
NsiI ATGCAT 1 cut(s) 1141
PaeR7I CTCGAG 1 cut(s) 1092
PagI TCATGA 2 cut(s) 942, 1441
PaqCI CACCTGC 1 cut(s) 419
PdmI GAANNNNTTC 2 cut(s) 225, 399
PfeI GAWTC 7 cut(s) 80, 353, 593, 605, 725, 1150, 1389
PflFI GACNNNGTC 2 cut(s) 74, 452
PkrI GCNGC 5 cut(s) 160, 439, 529, 1359, 1375
PleI GAGTC 4 cut(s) 83, 233, 355, 384
PpsI GAGTC 4 cut(s) 83, 233, 355, 384
Psp124BI GAGCTC 1 cut(s) 766
PspFI CCCAGC 2 cut(s) 4, 542
PspN4I GGNNCC 2 cut(s) 264, 455
PspPI GGNCC 1 cut(s) 454
PspXI VCTCGAGB 1 cut(s) 1092
PsyI GACNNNGTC 2 cut(s) 74, 452
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 4 cut(s) 264, 420, 744, 1477
RsaNI GTAC 4 cut(s) 263, 419, 743, 1476
RseI CAYNNNNRTG 3 cut(s) 873, 1038, 1446
SacI GAGCTC 1 cut(s) 766
SaqAI TTAA 6 cut(s) 21, 39, 164, 323, 513, 576
SatI GCNGC 5 cut(s) 159, 438, 528, 1358, 1374
Sau3AI GATC 4 cut(s) 817, 1030, 1231, 1333
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 4 cut(s) 84, 233, 356, 385
ScrFI CCNGG 1 cut(s) 908
SduI GDGCHC 1 cut(s) 766
SfaNI GCATC 2 cut(s) 1126, 1508
Sfr274I CTCGAG 1 cut(s) 1092
SinI GGWCC 1 cut(s) 454
SlaI CTCGAG 1 cut(s) 1092
SmiMI CAYNNNNRTG 3 cut(s) 873, 1038, 1446
SmlI CTYRAG 3 cut(s) 322, 1092, 1315
SmoI CTYRAG 3 cut(s) 322, 1092, 1315
Sse9I AATT 7 cut(s) 247, 406, 577, 720, 892, 1079, 1458
SsiI CCGC 2 cut(s) 461, 1421
SspI AATATT 2 cut(s) 634, 673
SspMI CTAG 1 cut(s) 231
SstI GAGCTC 1 cut(s) 766
StyD4I CCNGG 1 cut(s) 906
StyI CCWWGG 3 cut(s) 275, 411, 687
TaaI ACNGT 5 cut(s) 134, 552, 994, 1382, 1456
TaqI TCGA 3 cut(s) 78, 1093, 1234
TasI AATT 7 cut(s) 247, 406, 577, 720, 892, 1079, 1458
TatI WGTACW 1 cut(s) 418
TfiI GAWTC 7 cut(s) 80, 353, 593, 605, 725, 1150, 1389
Tru1I TTAA 6 cut(s) 21, 39, 164, 323, 513, 576
Tru9I TTAA 6 cut(s) 21, 39, 164, 323, 513, 576
TscAI CASTG 4 cut(s) 103, 555, 894, 985
TseFI GTSAC 3 cut(s) 68, 899, 1087
TseI GCWGC 5 cut(s) 158, 437, 527, 1357, 1373
Tsp45I GTSAC 3 cut(s) 68, 899, 1087
TspRI CASTG 4 cut(s) 103, 555, 894, 985
Tth111I GACNNNGTC 2 cut(s) 74, 452
Vha464I CTTAAG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 1 cut(s) 892
XcmI CCANNNNNNNNNTGG 3 cut(s) 178, 1054, 1348
XhoI CTCGAG 1 cut(s) 1092
XmiI GTMKAC 1 cut(s) 884
XmnI GAANNNNTTC 2 cut(s) 225, 399
XspI CTAG 1 cut(s) 231
Zsp2I ATGCAT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.