Rh4CG251700

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008027
Physical Location & Seq
Reverse (-)
50147024 .. 50153356
6333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG251700.1

Sequence Viewer

Length: 1173 bp
ATGCTGGGAGAAACTATGATTAAACCTGAAGGAGAACTTAAAGTAAGCTTTGGCTATCAATGCAGTAGTGACAGAGTCGATTCTTGTGAGGCTTCCAGTGGATATGAAATCCTGCCTGACCTCCGAAGGAACAGTAGTTTCTCTTGCTTGTCTGGAGCTGCGTTAAGTGCCAATGCCACTCTGGCAAACACTAATATCTGCAATGGTTTGATAGGGGAAGAAATACTTCCTAGTTGGGACTCCCCTAATTCATTTCGTAAGGTACCCTCTTCGCCAAGGTTGGATATATTATCATCTTCTCTTCAGAGTAGTATGTCCAACTTAAGTTGCAGTCCATCTACACCCGAGTCTGATTCTTACTTGATGAAATCCATGAGTCCTACTTCCAGAGGTGAAGGTTTTCTCAATTCCATGGAAGTACAAGTGGCAGGTGGAGCTGCTGGTGAAGACAGGGTCCAAGCGGTTTGTTCTGAAGAAAATGGGTGGCTGTATTGTGCAATATACGATGGCTTTAATGGAAGAGACGCAGCTGATTTTCTGGCTGGGACACTATATGAAACCATTGTATTTTACTTTAATTCATTAGACTGGGAATCAAATCAGGAATCCATCAATGCTTCTAAAGGGCTAAATATTGATGGGTCTCGTCCATTTCTTGAAGATGATAGTAATATTGAAAATATCTCCAAGGATGCTTCATCTACTGATGTAGAAATGTCAACTGATTCATTCAGGCATGGGGTACTTGATAGCCTACAACGAGCTCTTAGTCAGGCCGAGAATGATTTTCTATACATGGTTGAGCAGGAAATGGAGGATCGTCCTGATTTGGTTTCTGTTGGGTCTTGTGTTTTAGTTGTGCTTCTTCATGGGAATGATTTGTATACACTTAATTTAGGTGACAGCCGGGCTGTGTTGGCAACACTTGGTGATGGTGATTTCATGAATGGGAGTGAGAGACTGAAAGCTATCCAGCTCACTGAAAGTCATACAGTTGATGATGAAGTTGAAAGAATGCGAGTTTTGTGTGATCATCCTGATGACCCCATGTCCATTGTGGCTGGAAAAGTAAAAGGGAAATTGAAGGTCACTCGAGCTTTTGGAGTTGGTTACTTGAAAAAGGTGATTGTTCTTCTGTGCTATTTGGCTACTTCATTACCTGATGATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

390

Amino Acids

42.38

Weight (kDa)

4.37

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 243 - 374 5.9e-16 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 419
Acc65I GGTACC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 262
AccI GTMKAC 1 cut(s) 884
AciI CCGC 1 cut(s) 461
AclWI GGATC 1 cut(s) 825
AcuI CTGAAG 3 cut(s) 48, 287, 492
AdeI CACNNNGTG 1 cut(s) 929
AfaI GTAC 3 cut(s) 264, 420, 744
AflII CTTAAG 1 cut(s) 322
AgsI TTSAA 5 cut(s) 659, 677, 1010, 1084, 1117
AhdI GACNNNNNGTC 1 cut(s) 1048
AluBI AGCT 8 cut(s) 48, 158, 437, 530, 764, 968, 976, 1097
AluI AGCT 8 cut(s) 48, 158, 437, 530, 764, 968, 976, 1097
Alw21I GWGCWC 1 cut(s) 766
Alw26I GTCTC 3 cut(s) 516, 648, 952
AlwI GGATC 1 cut(s) 825
Ama87I CYCGRG 2 cut(s) 344, 1092
AoxI GGCC 1 cut(s) 774
ApeKI GCWGC 3 cut(s) 158, 437, 527
Asp700I GAANNNNTTC 2 cut(s) 225, 399
Asp718I GGTACC 1 cut(s) 262
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 1 cut(s) 908
AsuHPI GGTGA 6 cut(s) 404, 455, 911, 941, 947, 1135
AvaI CYCGRG 2 cut(s) 344, 1092
AvaII GGWCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 766
BbvI GCAGC 3 cut(s) 145, 424, 539
BccI CCATC 5 cut(s) 343, 500, 617, 632, 926
BclI TGATCA 1 cut(s) 1030
BcnI CCSGG 1 cut(s) 908
BcoDI GTCTC 3 cut(s) 516, 648, 952
BfaI CTAG 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 322
BfuAI ACCTGC 1 cut(s) 419
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 3 cut(s) 159, 438, 528
BlsI GCNGC 3 cut(s) 160, 439, 529
Bme1390I CCNGG 1 cut(s) 908
Bme18I GGWCC 1 cut(s) 454
BmeRI GACNNNNNGTC 1 cut(s) 1048
BmeT110I CYCGRG 2 cut(s) 344, 1092
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 2 cut(s) 264, 455
BmrFI CCNGG 1 cut(s) 908
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 1 cut(s) 682
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 453
BpmI CTGGAG 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 908
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 275, 411, 687
Bse1I ACTGG 2 cut(s) 96, 593
Bse3DI GCAATG 1 cut(s) 208
BseDI CCNNGG 3 cut(s) 275, 411, 687
BseGI GGATG 2 cut(s) 697, 1033
BseMI GCAATG 1 cut(s) 208
BseNI ACTGG 2 cut(s) 96, 593
BseXI GCAGC 3 cut(s) 145, 424, 539
BseYI CCCAGC 2 cut(s) 4, 542
BshFI GGCC 1 cut(s) 776
BshNI GGYRCC 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 766
BsiHKCI CYCGRG 2 cut(s) 344, 1092
BsiSI CCGG 1 cut(s) 907
BslFI GGGAC 2 cut(s) 251, 559
BsmAI GTCTC 3 cut(s) 516, 648, 952
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 2 cut(s) 251, 559
BsmI GAATGC 1 cut(s) 1020
BsnI GGCC 1 cut(s) 776
Bso31I GGTCTC 1 cut(s) 648
BsoBI CYCGRG 2 cut(s) 344, 1092
Bsp1286I GDGCHC 1 cut(s) 766
Bsp143I GATC 2 cut(s) 817, 1030
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 1 cut(s) 461
BspANI GGCC 1 cut(s) 776
BspHI TCATGA 1 cut(s) 942
BspLI GGNNCC 2 cut(s) 264, 455
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 1 cut(s) 825
BspT107I GGYRCC 1 cut(s) 262
BspTI CTTAAG 1 cut(s) 322
BspTNI GGTCTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 2 cut(s) 96, 593
BssECI CCNNGG 3 cut(s) 275, 411, 687
BssMI GATC 2 cut(s) 817, 1030
BssNAI GTATAC 1 cut(s) 885
BssT1I CCWWGG 3 cut(s) 275, 411, 687
Bst1107I GTATAC 1 cut(s) 885
Bst4CI ACNGT 2 cut(s) 134, 994
Bst6I CTCTTC 3 cut(s) 274, 306, 514
BstAFI CTTAAG 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 767
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 2 cut(s) 697, 1033
BstKTI GATC 2 cut(s) 820, 1033
BstMAI GTCTC 3 cut(s) 516, 648, 952
BstMBI GATC 2 cut(s) 817, 1030
BstMWI GCNNNNNNNGC 5 cut(s) 60, 167, 182, 434, 917
BstSCI CCNGG 1 cut(s) 906
BstV1I GCAGC 3 cut(s) 145, 424, 539
BstV2I GAAGAC 1 cut(s) 453
BstZ17I GTATAC 1 cut(s) 885
BsuRI GGCC 1 cut(s) 776
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 2 cut(s) 697, 1033
BtsIMutI CAGTG 2 cut(s) 103, 978
BveI ACCTGC 1 cut(s) 419
CciI TCATGA 1 cut(s) 942
Cfr13I GGNCC 1 cut(s) 454
CseI GACGC 1 cut(s) 533
Csp6I GTAC 3 cut(s) 263, 419, 743
CviAII CATG 7 cut(s) 373, 412, 737, 796, 869, 943, 1048
CviQI GTAC 3 cut(s) 263, 419, 743
DdeI CTNAG 1 cut(s) 767
DpnI GATC 2 cut(s) 819, 1032
DpnII GATC 2 cut(s) 817, 1030
DraIII CACNNNGTG 1 cut(s) 929
DriI GACNNNNNGTC 1 cut(s) 1048
Eam1104I CTCTTC 3 cut(s) 274, 306, 514
Eam1105I GACNNNNNGTC 1 cut(s) 1048
EarI CTCTTC 3 cut(s) 274, 306, 514
Ecl136II GAGCTC 1 cut(s) 764
Eco130I CCWWGG 3 cut(s) 275, 411, 687
Eco24I GRGCYC 1 cut(s) 766
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 764
Eco57I CTGAAG 3 cut(s) 48, 287, 492
Eco88I CYCGRG 2 cut(s) 344, 1092
EcoICRI GAGCTC 1 cut(s) 764
EcoT14I CCWWGG 3 cut(s) 275, 411, 687
EcoT38I GRGCYC 1 cut(s) 766
ErhI CCWWGG 3 cut(s) 275, 411, 687
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
FalI AAGNNNNNCTT 4 cut(s) 21, 53, 210, 242
FaqI GGGAC 2 cut(s) 251, 559
FatI CATG 7 cut(s) 372, 411, 736, 795, 868, 942, 1047
FbaI TGATCA 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 884
Fnu4HI GCNGC 3 cut(s) 159, 438, 528
FokI GGATG 2 cut(s) 704, 1020
FriOI GRGCYC 1 cut(s) 766
Fsp4HI GCNGC 3 cut(s) 159, 438, 528
FspBI CTAG 1 cut(s) 231
GluI GCNGC 3 cut(s) 159, 438, 528
GsaI CCCAGC 2 cut(s) 8, 546
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 1 cut(s) 776
HapII CCGG 1 cut(s) 907
HgaI GACGC 1 cut(s) 533
Hin1II CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HindIII AAGCTT 1 cut(s) 46
HinfI GANTC 9 cut(s) 75, 80, 239, 347, 353, 376, 593, 605, 725
HpaII CCGG 1 cut(s) 907
HphI GGTGA 6 cut(s) 404, 455, 911, 941, 947, 1135
Hpy166II GTNNAC 2 cut(s) 720, 885
Hpy188I TCNGA 4 cut(s) 125, 306, 352, 472
Hpy188III TCNNGA 7 cut(s) 153, 387, 602, 656, 824, 943, 1037
Hpy8I GTNNAC 2 cut(s) 720, 885
HpyAV CCTTC 4 cut(s) 23, 120, 389, 1078
HpyCH4III ACNGT 2 cut(s) 134, 994
HpyCH4V TGCA 4 cut(s) 63, 201, 330, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 167, 182, 434, 917
HpyF3I CTNAG 1 cut(s) 767
Hsp92II CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
KpnI GGTACC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 1030
Kzo9I GATC 2 cut(s) 817, 1030
LmnI GCTCC 2 cut(s) 155, 434
Lsp1109I GCAGC 3 cut(s) 145, 424, 539
LweI GCATC 1 cut(s) 682
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 4 cut(s) 68, 899, 1087, 1109
MalI GATC 2 cut(s) 819, 1032
MboI GATC 2 cut(s) 817, 1030
MhlI GDGCHC 1 cut(s) 766
MluCI AATT 5 cut(s) 247, 406, 577, 892, 1079
MlyI GAGTC 4 cut(s) 84, 233, 356, 385
MmeI TCCRAC 2 cut(s) 261, 342
MnlI CCTC 5 cut(s) 82, 131, 277, 383, 808
MroXI GAANNNNTTC 2 cut(s) 225, 399
MseI TTAA 7 cut(s) 21, 39, 164, 323, 513, 576, 891
MslI CAYNNNNRTG 2 cut(s) 873, 1038
MspA1I CMGCKG 1 cut(s) 530
MspCI CTTAAG 1 cut(s) 322
MspI CCGG 1 cut(s) 907
MspR9I CCNGG 1 cut(s) 908
Mva1269I GAATGC 1 cut(s) 1020
MwoI GCNNNNNNNGC 5 cut(s) 60, 167, 182, 434, 917
NciI CCSGG 1 cut(s) 908
NcoI CCATGG 1 cut(s) 411
NdeII GATC 2 cut(s) 817, 1030
NlaIII CATG 7 cut(s) 376, 415, 740, 799, 872, 946, 1051
NlaIV GGNNCC 2 cut(s) 264, 455
NmeAIII GCCGAG 1 cut(s) 802
NmuCI GTSAC 3 cut(s) 68, 899, 1087
PaeR7I CTCGAG 1 cut(s) 1092
PagI TCATGA 1 cut(s) 942
PaqCI CACCTGC 1 cut(s) 419
PctI GAATGC 1 cut(s) 1020
PdmI GAANNNNTTC 2 cut(s) 225, 399
PfeI GAWTC 5 cut(s) 80, 353, 593, 605, 725
PflFI GACNNNGTC 2 cut(s) 74, 452
PkrI GCNGC 3 cut(s) 160, 439, 529
PleI GAGTC 4 cut(s) 83, 233, 355, 384
PpsI GAGTC 4 cut(s) 83, 233, 355, 384
Psp124BI GAGCTC 1 cut(s) 766
PspFI CCCAGC 2 cut(s) 4, 542
PspN4I GGNNCC 2 cut(s) 264, 455
PspPI GGNCC 1 cut(s) 454
PspXI VCTCGAGB 1 cut(s) 1092
PsyI GACNNNGTC 2 cut(s) 74, 452
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 264, 420, 744
RsaNI GTAC 3 cut(s) 263, 419, 743
RseI CAYNNNNRTG 2 cut(s) 873, 1038
SacI GAGCTC 1 cut(s) 766
SaqAI TTAA 7 cut(s) 21, 39, 164, 323, 513, 576, 891
SatI GCNGC 3 cut(s) 159, 438, 528
Sau3AI GATC 2 cut(s) 817, 1030
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 4 cut(s) 84, 233, 356, 385
ScrFI CCNGG 1 cut(s) 908
SduI GDGCHC 1 cut(s) 766
SfaNI GCATC 1 cut(s) 682
Sfr274I CTCGAG 1 cut(s) 1092
SinI GGWCC 1 cut(s) 454
SlaI CTCGAG 1 cut(s) 1092
SmiMI CAYNNNNRTG 2 cut(s) 873, 1038
SmlI CTYRAG 2 cut(s) 322, 1092
SmoI CTYRAG 2 cut(s) 322, 1092
Sse9I AATT 5 cut(s) 247, 406, 577, 892, 1079
SsiI CCGC 1 cut(s) 461
SspI AATATT 2 cut(s) 634, 673
SspMI CTAG 1 cut(s) 231
SstI GAGCTC 1 cut(s) 766
StyD4I CCNGG 1 cut(s) 906
StyI CCWWGG 3 cut(s) 275, 411, 687
TaaI ACNGT 2 cut(s) 134, 994
TaqI TCGA 2 cut(s) 78, 1093
TasI AATT 5 cut(s) 247, 406, 577, 892, 1079
TatI WGTACW 1 cut(s) 418
TfiI GAWTC 5 cut(s) 80, 353, 593, 605, 725
Tru1I TTAA 7 cut(s) 21, 39, 164, 323, 513, 576, 891
Tru9I TTAA 7 cut(s) 21, 39, 164, 323, 513, 576, 891
TscAI CASTG 2 cut(s) 103, 985
TseFI GTSAC 3 cut(s) 68, 899, 1087
TseI GCWGC 3 cut(s) 158, 437, 527
Tsp45I GTSAC 3 cut(s) 68, 899, 1087
TspRI CASTG 2 cut(s) 103, 985
Tth111I GACNNNGTC 2 cut(s) 74, 452
Vha464I CTTAAG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 454
XcmI CCANNNNNNNNNTGG 2 cut(s) 178, 1054
XhoI CTCGAG 1 cut(s) 1092
XmiI GTMKAC 1 cut(s) 884
XmnI GAANNNNTTC 2 cut(s) 225, 399
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.