FvH4_4g22190

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010445
Physical Location & Seq
Reverse (-)
25096481 .. 25097701
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22190.t1

Sequence Viewer

Length: 558 bp
ATGAAGAACAAGGCTTCAGTTTTCTTGAAGCAGATCATCACCTTGCTGAGCTCCATAGCCAAAGCCAAGACAATGGCGATCAAGAACAAAACCAGTGCGGCCAAGGCTCGCCTCATAATGTTCTCTTTGGTCAAGAACAAGAAGGTCTTGATGGACACAGTCTCCCACAAGATTCATAACCTCCTCGGCCACCACGACAAGGACAGCAACCAAGGCGACGACGACCGGGCCATTGTGCTCTACGCCAACCAGTACTCGCATGCCGCTGGCTCGAGCCGCCTGCACACCATTGAAGACGAGGAGGAGGATGACAATGACAAGTACCCCGATCTGAGGCACTGCCTCTTCGACGAGGACGAAAACTTTGATGACATCAAGGCTGGTGGGTCTGTGGTTGATATTGTCAAGAATTCAAGAGTGCAAGGACAGGATTTCAAGCTGGAAGATGAGATAGATCAAGTTGCGGACTTGTTCATCACGAGGTTTCATAAACAGATCCGCTTGCAGAAGCTTGAGTCCTTCAAGAGGTACAAAGACATGCTCGAGAGAAGCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

186

Amino Acids

21.24

Weight (kDa)

6.71

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 148 - 183 1.2e-15 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 98, 264, 277, 464, 499
AclWI GGATC 1 cut(s) 490
AcoI YGGCCR 2 cut(s) 99, 187
AcsI RAATTY 1 cut(s) 409
AfaI GTAC 3 cut(s) 254, 323, 530
AfiI CCNNNNNNNGG 3 cut(s) 199, 333, 525
AgsI TTSAA 5 cut(s) 28, 293, 414, 436, 523
AluBI AGCT 3 cut(s) 51, 439, 511
AluI AGCT 3 cut(s) 51, 439, 511
Alw21I GWGCWC 2 cut(s) 53, 240
Alw26I GTCTC 1 cut(s) 166
AlwI GGATC 1 cut(s) 490
Ama87I CYCGRG 2 cut(s) 271, 542
AoxI GGCC 3 cut(s) 99, 187, 228
ApoI RAATTY 1 cut(s) 409
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspS9I GGNCC 1 cut(s) 228
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 2 cut(s) 271, 542
BanII GRGCYC 1 cut(s) 53
BauI CACGAG 1 cut(s) 478
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 2 cut(s) 53, 240
BccI CCATC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 166
BfaI CTAG 1 cut(s) 556
BisI GCNGC 3 cut(s) 99, 264, 277
BlpI GCTNAGC 1 cut(s) 47
BlsI GCNGC 3 cut(s) 100, 265, 278
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 1 cut(s) 227
BmeT110I CYCGRG 2 cut(s) 271, 542
BmgT120I GGNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 300
Bpu1102I GCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 102, 184, 211
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 333, 525
Bse1I ACTGG 2 cut(s) 93, 250
BseDI CCNNGG 3 cut(s) 102, 184, 211
BseGI GGATG 1 cut(s) 313
BseLI CCNNNNNNNGG 3 cut(s) 199, 333, 525
BseMII CTCAG 2 cut(s) 38, 323
BseNI ACTGG 2 cut(s) 93, 250
BseRI GAGGAG 3 cut(s) 173, 314, 317
BsgI GTGCAG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 101, 189, 230
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 53, 240
BsiHKCI CYCGRG 2 cut(s) 271, 542
BsiSI CCGG 1 cut(s) 226
BslI CCNNNNNNNGG 3 cut(s) 199, 333, 525
BsmAI GTCTC 1 cut(s) 166
BsnI GGCC 3 cut(s) 101, 189, 230
BsoBI CYCGRG 2 cut(s) 271, 542
Bsp1286I GDGCHC 2 cut(s) 53, 240
Bsp143I GATC 5 cut(s) 33, 78, 328, 454, 495
Bsp1720I GCTNAGC 1 cut(s) 47
BspACI CCGC 5 cut(s) 98, 264, 277, 464, 499
BspANI GGCC 3 cut(s) 101, 189, 230
BspCNI CTCAG 2 cut(s) 39, 324
BspPI GGATC 1 cut(s) 490
BsrI ACTGG 2 cut(s) 93, 250
BssECI CCNNGG 3 cut(s) 102, 184, 211
BssMI GATC 5 cut(s) 33, 78, 328, 454, 495
BssSI CACGAG 1 cut(s) 478
BssT1I CCWWGG 2 cut(s) 102, 211
Bst2BI CACGAG 1 cut(s) 478
Bst4CI ACNGT 1 cut(s) 160
Bst6I CTCTTC 1 cut(s) 350
BstC8I GCNNGC 5 cut(s) 109, 261, 268, 281, 503
BstDEI CTNAG 2 cut(s) 47, 332
BstENI CCTNNNNNAGG 1 cut(s) 523
BstF5I GGATG 1 cut(s) 313
BstKTI GATC 5 cut(s) 36, 81, 331, 457, 498
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 5 cut(s) 33, 78, 328, 454, 495
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 3 cut(s) 104, 213, 276
BstNSI RCATGY 2 cut(s) 263, 541
BstSCI CCNGG 1 cut(s) 225
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 495
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 1 cut(s) 495
BsuRI GGCC 3 cut(s) 101, 189, 230
BtsCI GGATG 1 cut(s) 313
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 100, 337
Cac8I GCNNGC 5 cut(s) 109, 261, 268, 281, 503
Cfr13I GGNCC 1 cut(s) 228
Csp6I GTAC 3 cut(s) 253, 322, 529
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviAII CATG 2 cut(s) 260, 538
CviQI GTAC 3 cut(s) 253, 322, 529
DdeI CTNAG 2 cut(s) 47, 332
DpnI GATC 5 cut(s) 35, 80, 330, 456, 497
DpnII GATC 5 cut(s) 33, 78, 328, 454, 495
EaeI YGGCCR 2 cut(s) 99, 187
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 102, 211
Eco24I GRGCYC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 51
Eco88I CYCGRG 2 cut(s) 271, 542
EcoICRI GAGCTC 1 cut(s) 51
EcoNI CCTNNNNNAGG 1 cut(s) 523
EcoRI GAATTC 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 102, 211
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 102, 211
FaeI CATG 2 cut(s) 263, 541
FaiI YATR 6 cut(s) 56, 116, 177, 261, 489, 539
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FatI CATG 2 cut(s) 259, 537
Fnu4HI GCNGC 3 cut(s) 99, 264, 277
FokI GGATG 1 cut(s) 320
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 3 cut(s) 99, 264, 277
FspBI CTAG 1 cut(s) 556
GluI GCNGC 3 cut(s) 99, 264, 277
HaeIII GGCC 3 cut(s) 101, 189, 230
HapII CCGG 1 cut(s) 226
Hin1II CATG 2 cut(s) 263, 541
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 2 cut(s) 172, 515
HpaII CCGG 1 cut(s) 226
HphI GGTGA 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 333
Hpy188III TCNNGA 9 cut(s) 25, 82, 133, 148, 406, 414, 478, 523, 544
Hpy99I CGWCG 3 cut(s) 221, 224, 353
HpyAV CCTTC 2 cut(s) 136, 529
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 283, 421, 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 213, 276
HpyF3I CTNAG 2 cut(s) 47, 332
Hsp92II CATG 2 cut(s) 263, 541
Kzo9I GATC 5 cut(s) 33, 78, 328, 454, 495
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 8 cut(s) 106, 239, 252, 263, 293, 366, 413, 425
MaeI CTAG 1 cut(s) 556
MalI GATC 5 cut(s) 35, 80, 330, 456, 497
MboI GATC 5 cut(s) 33, 78, 328, 454, 495
MboII GAAGA 4 cut(s) 16, 305, 337, 455
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 2 cut(s) 53, 240
MluCI AATT 1 cut(s) 409
MlyI GAGTC 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 226
MspR9I CCNGG 1 cut(s) 227
MwoI GCNNNNNNNGC 3 cut(s) 104, 213, 276
NciI CCSGG 1 cut(s) 227
NdeII GATC 5 cut(s) 33, 78, 328, 454, 495
NlaIII CATG 2 cut(s) 263, 541
NmeAIII GCCGAG 1 cut(s) 165
NspI RCATGY 2 cut(s) 263, 541
PaeI GCATGC 1 cut(s) 263
PaeR7I CTCGAG 2 cut(s) 271, 542
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 354
PfeI GAWTC 1 cut(s) 172
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 3 cut(s) 100, 265, 278
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
Psp124BI GAGCTC 1 cut(s) 53
PspPI GGNCC 1 cut(s) 228
PspXI VCTCGAGB 1 cut(s) 271
PsuI RGATCY 1 cut(s) 495
PsyI GACNNNGTC 1 cut(s) 158
RsaI GTAC 3 cut(s) 254, 323, 530
RsaNI GTAC 3 cut(s) 253, 322, 529
SacI GAGCTC 1 cut(s) 53
SatI GCNGC 3 cut(s) 99, 264, 277
Sau3AI GATC 5 cut(s) 33, 78, 328, 454, 495
Sau96I GGNCC 1 cut(s) 228
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 2 cut(s) 53, 240
SetI ASST 9 cut(s) 44, 53, 147, 183, 441, 485, 513, 530, 557
Sfr274I CTCGAG 2 cut(s) 271, 542
SlaI CTCGAG 2 cut(s) 271, 542
SmlI CTYRAG 3 cut(s) 271, 512, 542
SmoI CTYRAG 3 cut(s) 271, 512, 542
SphI GCATGC 1 cut(s) 263
Sse9I AATT 1 cut(s) 409
SsiI CCGC 5 cut(s) 98, 264, 277, 464, 499
SspMI CTAG 1 cut(s) 556
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 2 cut(s) 102, 211
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 272, 348, 543
TasI AATT 1 cut(s) 409
TatI WGTACW 1 cut(s) 252
TauI GCSGC 3 cut(s) 101, 266, 279
TfiI GAWTC 1 cut(s) 172
TscAI CASTG 2 cut(s) 100, 344
TspDTI ATGAA 4 cut(s) 17, 164, 463, 476
TspRI CASTG 2 cut(s) 100, 344
Tth111I GACNNNGTC 1 cut(s) 158
XagI CCTNNNNNAGG 1 cut(s) 523
XapI RAATTY 1 cut(s) 409
XceI RCATGY 2 cut(s) 263, 541
XhoI CTCGAG 2 cut(s) 271, 542
XspI CTAG 1 cut(s) 556
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.