MD16G1144600.v1.1

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010445
Physical Location & Seq
Forward (+)
11127039 .. 11127632
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1144600.v1.1.491

Sequence Viewer

Length: 594 bp
ATGAAGAACAAGGCTTCTGTTTTCTTGAAACATATAATCACCTTGCTGAGTTCCATAGCCAAGGCCAAGTCCTTGGCTATCAAGAGCAAAACTAGCGCTGCGAAAGCTCGCCTGATAATGTTCTCATTGGTGAAGAACAAGAAGGTTTTGATGGACACCGTGTCACACAAGATCCACAGTCTCCTAGGCCACGATCACCGCGATCAAGAGGAGGAGGAGGGAGACCAAAGCAAGGCGCTTGTTCTCTACAATGCCATTGCCAATGAGTACTCACATGCAGTTGGTACTTCGATCTGCATGCTTAGGTACAATGGCCAAGATGAGGAGGAGGAGGAGGACGACGACGACGATGATAACTACCCTGATCTCAGGCACTCATTGTTCGATGAAAATGAGAACTTTGATGATGTCAAGGATGGCGGGTCTGTGATCGATCTGGTGAAGAATTCCAAGGTGGAAGGAGAGGATTTCAAGCTGGAAGACGAGATAGATCACGTTGCGGATTTGTTCATTAATAGGTTCCATAAACAAATGCGCTTGCAAAAGCTTCTCTCCTTCAAAAGGTATCAAGAAATGATCAATAGAAGCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

198

Amino Acids

22.63

Weight (kDa)

5.37

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 160 - 195 4.9e-17 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 201
AciI CCGC 3 cut(s) 199, 420, 500
AclWI GGATC 1 cut(s) 166
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 1 cut(s) 445
AfaI GTAC 3 cut(s) 269, 286, 308
AfeI AGCGCT 1 cut(s) 97
AfiI CCNNNNNNNGG 3 cut(s) 232, 322, 561
AgsI TTSAA 3 cut(s) 28, 472, 559
AhdI GACNNNNNGTC 1 cut(s) 160
AluBI AGCT 3 cut(s) 107, 475, 547
AluI AGCT 3 cut(s) 107, 475, 547
Alw26I GTCTC 2 cut(s) 185, 216
AlwI GGATC 1 cut(s) 166
Aor51HI AGCGCT 1 cut(s) 97
AoxI GGCC 3 cut(s) 63, 187, 313
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 445
AseI ATTAAT 1 cut(s) 513
AspA2I CCTAGG 1 cut(s) 184
AspLEI GCGC 3 cut(s) 98, 238, 537
AsuHPI GGTGA 4 cut(s) 31, 142, 188, 451
AvrII CCTAGG 1 cut(s) 184
BalI TGGCCA 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 85
BccI CCATC 2 cut(s) 145, 410
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BclI TGATCA 1 cut(s) 576
BcoDI GTCTC 2 cut(s) 185, 216
BfaI CTAG 2 cut(s) 93, 185
BfoI RGCGCY 2 cut(s) 99, 239
BisI GCNGC 1 cut(s) 99
BlnI CCTAGG 1 cut(s) 184
BlsI GCNGC 1 cut(s) 100
BmcAI AGTACT 1 cut(s) 269
BmeRI GACNNNNNGTC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 302
Bsa29I ATCGAT 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 216
BsaJI CCNNGG 4 cut(s) 60, 72, 184, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 322, 561
Bse3DI GCAATG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 4 cut(s) 60, 72, 184, 450
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 3 cut(s) 232, 322, 561
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 38, 382
BseRI GAGGAG 7 cut(s) 224, 227, 230, 338, 341, 344, 347
BseXI GCAGC 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 3 cut(s) 65, 189, 315
BshVI ATCGAT 1 cut(s) 432
BslI CCNNNNNNNGG 3 cut(s) 232, 322, 561
BsmAI GTCTC 2 cut(s) 185, 216
BsnI GGCC 3 cut(s) 65, 189, 315
Bso31I GGTCTC 1 cut(s) 216
Bsp143I GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
BspACI CCGC 3 cut(s) 199, 420, 500
BspANI GGCC 3 cut(s) 65, 189, 315
BspCNI CTCAG 2 cut(s) 39, 381
BspDI ATCGAT 1 cut(s) 432
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 521
BspPI GGATC 1 cut(s) 166
BspTNI GGTCTC 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 4 cut(s) 60, 72, 184, 450
BssMI GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
BssT1I CCWWGG 4 cut(s) 60, 72, 184, 450
Bst4CI ACNGT 2 cut(s) 160, 179
BstC8I GCNNGC 3 cut(s) 109, 299, 539
BstDEI CTNAG 3 cut(s) 47, 302, 368
BstENI CCTNNNNNAGG 1 cut(s) 559
BstF5I GGATG 1 cut(s) 421
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 2 cut(s) 99, 239
BstHHI GCGC 3 cut(s) 98, 238, 537
BstKTI GATC 9 cut(s) 174, 196, 205, 294, 367, 432, 436, 493, 579
BstMAI GTCTC 2 cut(s) 185, 216
BstMBI GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
BstMWI GCNNNNNNNGC 2 cut(s) 93, 104
BstNSI RCATGY 2 cut(s) 278, 301
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 85
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 432
BsuRI GGCC 3 cut(s) 65, 189, 315
BsuTUI ATCGAT 1 cut(s) 432
BtsCI GGATG 1 cut(s) 421
Cac8I GCNNGC 3 cut(s) 109, 299, 539
CfoI GCGC 3 cut(s) 98, 238, 537
ClaI ATCGAT 1 cut(s) 432
CseI GACGC 1 cut(s) 577
Csp6I GTAC 3 cut(s) 268, 285, 307
CviAII CATG 2 cut(s) 275, 298
CviJI RGCY 9 cut(s) 14, 59, 65, 77, 107, 189, 315, 475, 547
CviKI_1 RGCY 9 cut(s) 14, 59, 65, 77, 107, 189, 315, 475, 547
CviQI GTAC 3 cut(s) 268, 285, 307
DdeI CTNAG 3 cut(s) 47, 302, 368
DpnI GATC 9 cut(s) 173, 195, 204, 293, 366, 431, 435, 492, 578
DpnII GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
DriI GACNNNNNGTC 1 cut(s) 160
EaeI YGGCCR 1 cut(s) 313
Eam1105I GACNNNNNGTC 1 cut(s) 160
Eco130I CCWWGG 4 cut(s) 60, 72, 184, 450
Eco31I GGTCTC 1 cut(s) 216
Eco47III AGCGCT 1 cut(s) 97
EcoNI CCTNNNNNAGG 1 cut(s) 559
EcoRI GAATTC 1 cut(s) 445
EcoT14I CCWWGG 4 cut(s) 60, 72, 184, 450
ErhI CCWWGG 4 cut(s) 60, 72, 184, 450
FaeI CATG 2 cut(s) 278, 301
FaiI YATR 6 cut(s) 33, 35, 56, 276, 299, 525
FatI CATG 2 cut(s) 274, 297
FauI CCCGC 1 cut(s) 413
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 2 cut(s) 93, 185
GlaI GCGC 3 cut(s) 97, 237, 536
GluI GCNGC 1 cut(s) 99
HaeII RGCGCY 2 cut(s) 99, 239
HaeIII GGCC 3 cut(s) 65, 189, 315
HgaI GACGC 1 cut(s) 577
HhaI GCGC 3 cut(s) 98, 238, 537
Hin1II CATG 2 cut(s) 278, 301
Hin6I GCGC 3 cut(s) 96, 236, 535
HinP1I GCGC 3 cut(s) 96, 236, 535
HindIII AAGCTT 1 cut(s) 545
HphI GGTGA 4 cut(s) 31, 142, 188, 451
Hpy188I TCNGA 1 cut(s) 593
Hpy188III TCNNGA 4 cut(s) 25, 82, 206, 569
Hpy99I CGWCG 3 cut(s) 344, 347, 350
HpyAV CCTTC 3 cut(s) 136, 452, 565
HpyCH4III ACNGT 2 cut(s) 160, 179
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 3 cut(s) 278, 297, 541
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 104
HpyF3I CTNAG 3 cut(s) 47, 302, 368
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 2 cut(s) 278, 301
HspAI GCGC 3 cut(s) 96, 236, 535
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
LpnPI CCDG 5 cut(s) 125, 355, 375, 422, 461
Lsp1109I GCAGC 1 cut(s) 85
MaeI CTAG 2 cut(s) 93, 185
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 162
MalI GATC 9 cut(s) 173, 195, 204, 293, 366, 431, 435, 492, 578
MboI GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
MboII GAAGA 4 cut(s) 16, 145, 454, 491
MflI RGATCY 1 cut(s) 171
MlsI TGGCCA 1 cut(s) 315
MluCI AATT 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 315
Mox20I TGGCCA 1 cut(s) 315
MscI TGGCCA 1 cut(s) 315
MseI TTAA 1 cut(s) 513
Msp20I TGGCCA 1 cut(s) 315
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 2 cut(s) 93, 104
NdeII GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
NlaIII CATG 2 cut(s) 278, 301
NlaIV GGNNCC 1 cut(s) 521
NmuCI GTSAC 1 cut(s) 162
NspI RCATGY 2 cut(s) 278, 301
PaeI GCATGC 1 cut(s) 301
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 345
PkrI GCNGC 1 cut(s) 100
PshBI ATTAAT 1 cut(s) 513
PspN4I GGNNCC 1 cut(s) 521
PsuI RGATCY 1 cut(s) 171
RsaI GTAC 3 cut(s) 269, 286, 308
RsaNI GTAC 3 cut(s) 268, 285, 307
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 9 cut(s) 171, 193, 202, 291, 364, 429, 433, 490, 576
ScaI AGTACT 1 cut(s) 269
SphI GCATGC 1 cut(s) 301
Sse9I AATT 1 cut(s) 445
SsiI CCGC 3 cut(s) 199, 420, 500
SspMI CTAG 2 cut(s) 93, 185
StyI CCWWGG 4 cut(s) 60, 72, 184, 450
TaaI ACNGT 2 cut(s) 160, 179
TaiI ACGT 1 cut(s) 498
TaqI TCGA 3 cut(s) 290, 384, 432
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 267
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TseFI GTSAC 1 cut(s) 162
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 162
TspDTI ATGAA 3 cut(s) 17, 402, 499
VspI ATTAAT 1 cut(s) 513
XagI CCTNNNNNAGG 1 cut(s) 559
XapI RAATTY 1 cut(s) 445
XceI RCATGY 2 cut(s) 278, 301
XmaJI CCTAGG 1 cut(s) 184
XspI CTAG 2 cut(s) 93, 185
ZrmI AGTACT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.