MD13G1134000.v1.1

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010445
Physical Location & Seq
Forward (+)
10265171 .. 10265758
588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1134000.v1.1.491

Sequence Viewer

Length: 588 bp
ATGAAGAATAAGGCTTCTGTTTTCTTGAAGCAGATAATCACATTGTTGAGCTCAATAGCCAAAGCCAAGTCCCTCGCCATCAAGAGCAAAACCAGCGCCGCTAAAGCTCGCCTGATAATGTTTTCATTGGTGAAGAACAAGAAGGTTTTGATGGACACTGTGTCGCACAAGATCCATAGCCTTTTAGGTCACGATCACCGTGATCAAGGGGAGGAGGAGGAAGGAGACCAAAACAAGGCGCTTGTTCTCTACAATGCCATGGCCAATGAGTACTCGCATACAGCTGGTACTTCAAGCTACATGCGAAGGTACAATGGTGAAGATGAGGAGGAGGACGATGACGATGATAAGTACCCTGATCTCACTCACTCGTTGTTCGATGAAAATGAGAACTTTGACGATGTCAAAGCTGGCGGGTCTGTGATCGATCTGGTGAAGAATTCCAAAGAGGAAGGACAGGATTTCAAGCTGGAAGACGAGATAGACCACGTTGCGGATTTGTTCATTAACAGGTTTCACAAACAAATGCGCTTGCAAAAGCTTCTATCCTTCAAAAGGCATCAAGAAATGCTCGACAGAAGCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

196

Amino Acids

22.35

Weight (kDa)

5.72

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 158 - 193 1.7e-15 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
AciI CCGC 3 cut(s) 99, 414, 494
AclWI GGATC 1 cut(s) 166
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 4 cut(s) 272, 289, 311, 353
AfiI CCNNNNNNNGG 3 cut(s) 235, 493, 555
AgsI TTSAA 4 cut(s) 28, 294, 466, 553
AhdI GACNNNNNGTC 1 cut(s) 160
AluBI AGCT 7 cut(s) 51, 107, 284, 297, 410, 469, 541
AluI AGCT 7 cut(s) 51, 107, 284, 297, 410, 469, 541
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 1 cut(s) 219
AlwI GGATC 1 cut(s) 166
AoxI GGCC 1 cut(s) 261
ApoI RAATTY 1 cut(s) 439
AspLEI GCGC 3 cut(s) 98, 241, 531
AsuHPI GGTGA 4 cut(s) 142, 188, 329, 445
BaeI ACNNNNGTAYC 2 cut(s) 301, 334
BalI TGGCCA 1 cut(s) 263
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 53
BccI CCATC 2 cut(s) 86, 145
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 219
BfoI RGCGCY 2 cut(s) 99, 242
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
BmcAI AGTACT 1 cut(s) 272
BmeRI GACNNNNNGTC 1 cut(s) 160
BmsI GCATC 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 480
Bsa29I ATCGAT 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 219
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 493, 555
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 235, 493, 555
BseRI GAGGAG 4 cut(s) 227, 230, 341, 344
BshFI GGCC 1 cut(s) 263
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 53
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 3 cut(s) 235, 493, 555
BsmAI GTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 55
BsnI GGCC 1 cut(s) 263
Bso31I GGTCTC 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 6 cut(s) 171, 193, 202, 358, 423, 427
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 3 cut(s) 99, 414, 494
BspANI GGCC 1 cut(s) 263
BspDI ATCGAT 1 cut(s) 426
BspPI GGATC 1 cut(s) 166
BspTNI GGTCTC 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 6 cut(s) 171, 193, 202, 358, 423, 427
BssT1I CCWWGG 1 cut(s) 258
Bst4CI ACNGT 2 cut(s) 160, 200
BstC8I GCNNGC 3 cut(s) 109, 412, 533
BstDSI CCRYGG 1 cut(s) 258
BstENI CCTNNNNNAGG 1 cut(s) 553
BstH2I RGCGCY 2 cut(s) 99, 242
BstHHI GCGC 3 cut(s) 98, 241, 531
BstKTI GATC 6 cut(s) 174, 196, 205, 361, 426, 430
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 6 cut(s) 171, 193, 202, 358, 423, 427
BstMWI GCNNNNNNNGC 2 cut(s) 93, 104
BstNSI RCATGY 1 cut(s) 304
BstV2I GAAGAC 1 cut(s) 480
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 1 cut(s) 263
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 3 cut(s) 109, 412, 533
CfoI GCGC 3 cut(s) 98, 241, 531
ClaI ATCGAT 1 cut(s) 426
CseI GACGC 1 cut(s) 571
Csp6I GTAC 4 cut(s) 271, 288, 310, 352
CviAII CATG 2 cut(s) 259, 301
CviQI GTAC 4 cut(s) 271, 288, 310, 352
DpnI GATC 6 cut(s) 173, 195, 204, 360, 425, 429
DpnII GATC 6 cut(s) 171, 193, 202, 358, 423, 427
DrdI GACNNNNNNGTC 1 cut(s) 581
DriI GACNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 160
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 51
EcoNI CCTNNNNNAGG 1 cut(s) 553
EcoRI GAATTC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 2 cut(s) 262, 304
FaiI YATR 4 cut(s) 177, 260, 279, 302
FaqI GGGAC 1 cut(s) 55
FatI CATG 2 cut(s) 258, 300
FauI CCCGC 1 cut(s) 407
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 1 cut(s) 99
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 99
GlaI GCGC 3 cut(s) 97, 240, 530
GluI GCNGC 1 cut(s) 99
HaeII RGCGCY 2 cut(s) 99, 242
HaeIII GGCC 1 cut(s) 263
HgaI GACGC 1 cut(s) 571
HhaI GCGC 3 cut(s) 98, 241, 531
Hin1II CATG 2 cut(s) 262, 304
Hin6I GCGC 3 cut(s) 96, 239, 529
HinP1I GCGC 3 cut(s) 96, 239, 529
HindIII AAGCTT 1 cut(s) 539
HphI GGTGA 4 cut(s) 142, 188, 329, 445
Hpy188I TCNGA 1 cut(s) 587
Hpy188III TCNNGA 4 cut(s) 25, 82, 191, 563
HpyAV CCTTC 5 cut(s) 136, 215, 300, 446, 559
HpyCH4III ACNGT 2 cut(s) 160, 200
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 1 cut(s) 535
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 104
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 2 cut(s) 262, 304
HspAI GCGC 3 cut(s) 96, 239, 529
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 6 cut(s) 171, 193, 202, 358, 423, 427
LpnPI CCDG 9 cut(s) 106, 125, 270, 369, 396, 416, 443, 455, 496
LweI GCATC 1 cut(s) 568
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 1 cut(s) 188
MalI GATC 6 cut(s) 173, 195, 204, 360, 425, 429
MboI GATC 6 cut(s) 171, 193, 202, 358, 423, 427
MboII GAAGA 5 cut(s) 16, 145, 332, 448, 485
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 1 cut(s) 53
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 1 cut(s) 439
MluNI TGGCCA 1 cut(s) 263
MnlI CCTC 8 cut(s) 83, 205, 208, 211, 319, 322, 325, 442
Mox20I TGGCCA 1 cut(s) 263
MscI TGGCCA 1 cut(s) 263
MseI TTAA 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 284
MwoI GCNNNNNNNGC 2 cut(s) 93, 104
NcoI CCATGG 1 cut(s) 258
NdeII GATC 6 cut(s) 171, 193, 202, 358, 423, 427
NlaIII CATG 2 cut(s) 262, 304
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 579
PflFI GACNNNGTC 1 cut(s) 401
PkrI GCNGC 1 cut(s) 100
Psp124BI GAGCTC 1 cut(s) 53
PsuI RGATCY 1 cut(s) 171
PsyI GACNNNGTC 1 cut(s) 401
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 4 cut(s) 272, 289, 311, 353
RsaNI GTAC 4 cut(s) 271, 288, 310, 352
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 6 cut(s) 171, 193, 202, 358, 423, 427
ScaI AGTACT 1 cut(s) 272
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 1 cut(s) 568
Sse9I AATT 1 cut(s) 439
SsiI CCGC 3 cut(s) 99, 414, 494
SstI GAGCTC 1 cut(s) 53
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 2 cut(s) 160, 200
TaiI ACGT 1 cut(s) 492
TaqI TCGA 3 cut(s) 378, 426, 573
TasI AATT 1 cut(s) 439
TatI WGTACW 1 cut(s) 270
TauI GCSGC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 4 cut(s) 17, 114, 396, 493
TspRI CASTG 1 cut(s) 163
Tth111I GACNNNGTC 1 cut(s) 401
XagI CCTNNNNNAGG 1 cut(s) 553
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 304
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.